@article{SebaldBuecherOlbrichetal.1968, author = {Sebald, Walter and B{\"u}cher, T. and Olbrich, B. and Kaudewitz, F.}, title = {Electrophoretic pattern of and amino acid incorporation in vitro into the insoluble mitochondrial protein of neurospora crassa wild type and mi-1 mutant}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62926}, year = {1968}, abstract = {No abstract available}, subject = {Biochemie}, language = {en} } @article{NeupertSebaldSchwabetal.1969, author = {Neupert, W. and Sebald, Walter and Schwab, A. J. and Pfaller, A. and B{\"u}cher, T.}, title = {Puromycin sensitivity of ribosomal label after incorporation of \(^{14}\)C-labelled amino acids into isolated mitochondria from Neurospora crassa}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62899}, year = {1969}, abstract = {Radioactive amino acids were incorporated into isolated mitochondria from Neurospora crassa. Then the mitochondrial ribosomes were isolated and submitted to density gradient centrifugation. A preferential labelling of polysomes was observed. However, when the mitochondrial suspension was treated with puromycin after amino acid incorporation, no radioactivity could be detected in either the monosomes or the polysomes. The conclusion is drawn that isolated mitochondria under these conditions do not incorporate significant amounts of amino acids into proteins of their ribosomes.}, subject = {Biochemie}, language = {en} } @article{SebaldSchwabBuecher1969, author = {Sebald, Walter and Schwab, A. J. and B{\"u}cher, T.}, title = {Cycloheximide resistant amino acid incorporation into mitochondrial protein from Neurospora crassa in vivo}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62900}, year = {1969}, abstract = {No abstract available}, subject = {Biochemie}, language = {en} } @article{SebaldHofstoetterHackeretal.1969, author = {Sebald, Walter and Hofst{\"o}tter, T. and Hacker, D. and B{\"u}cher, T.}, title = {Incorporation of amino acids into mitochondrial protein of the flight muscle of Locusta migratoria in vitro and in vivo in the presence of cycloheximide}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62919}, year = {1969}, abstract = {No abstract available}, subject = {Biochemie}, language = {en} } @article{NeupertSebaldSchwabetal.1969, author = {Neupert, W. and Sebald, Walter and Schwab, A. J. and Massinger, P. and B{\"u}cher, T.}, title = {Incorporation in vivo of \(^{14}\)C-labelled amino acids into the proteins of mitochondrial ribosomes from Neurospora crassa sensitive to cycloheximide and insensitive to Chloramphenicol}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62884}, year = {1969}, abstract = {Radioactive amino acids were incorporated in vivo into N eurospora crassa cells, and the mitochondrial ribosomes were isolated. The incorporation of radioactivity into the proteins of these ribosomes was inhibited by cycloheximide, but not by chloramphenicol. It is therefore concluded that these proteins are synthesized on the cycloheximide sensitive and chloramphenicol insensitive cytoplasmic ribosomes.}, subject = {Biochemie}, language = {en} } @article{ScheerFranke1969, author = {Scheer, Ulrich and Franke, Werner W.}, title = {Negative staining and adenosine triphosphatase activity of annulate lamellae of newt oocytes}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32087}, year = {1969}, abstract = {Semi -iso la ted annul a te lamellae were prepared from single newt oocy tes (Triturus alpestris) by a modified Call a n-T omlin technique. Such preparations were examined with the electron mi croscope, and the negative sta ining a ppearance of th e a nnulate lamellae is described . The annul a te lamellae can be de tected either adhering to the nuclear envelope or being detached from it. Sometimes they a re obse rved to be connected with slender tubular-like structures interpreted as pa rts of the endoplasmic reti culum. The results obta ined from negativ e sta ining a re combined with those from sections. Especially, the structural data on th e a nnula te lamellae and the nuclear envelope of the very same cell were compa red . Evidence is presented th a t in the oocytes studied the two kinds of porous cisternae, n amely a nnul a te lamellae and nuclear envelope, a re markedly distinguished in that the annul a te lamellae ex hibit a much higher pore frequency (generally about twice tha t found for the corresponding nuclear envelope) and have al so a rela tive pore area occupying as much as 32 \% to 55 \% of th e cistern al surface (compa red with 13 \% to 22 \% in the nuclear envelopes). T he pore di ame ter a nd all other ultras tructural details of the pore complexes, however, a re equi valent in both kinds of porous cisternae. Like the annuli of the nuclear pore complexes of various a nimal and pl ant cells, the a nnuli of the a nnula te lamellae pores reveal al so an eightfold symmetry of their subunits in negatively stained as well as in ectioned ma teria l. Furthermore, th e a nnul a te lamellae a re shown to be a site of activity of the Mg-Na-Kstimul a ted ATPase.}, language = {en} } @article{SebaldNeupertBirkmayer1970, author = {Sebald, Walter and Neupert, W. and Birkmayer, G. D.}, title = {Internal and external contributions to the biogenesis of mitochondrial proteins}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62876}, year = {1970}, abstract = {No abstract available}, subject = {Biochemie}, language = {en} } @article{Scheer1970, author = {Scheer, Ulrich}, title = {The ultrastructure of the nuclear envelope of amphibian oocytes: a reinvestigation. III. Actinomycin D-induced decrease in central granules within the pores.}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32110}, year = {1970}, abstract = {No abstract available}, language = {en} } @article{FrankeScheer1970, author = {Franke, Werner W. and Scheer, Ulrich}, title = {The ultrastructure of the nuclear envelope of amphibian oocytes: a reinvestigation. II. The immature oocyte and dynamic aspects}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32102}, year = {1970}, abstract = {No abstract available}, language = {en} } @article{BeckerNath1970, author = {Becker, Charles R. and Nath, G.}, title = {Optical properties of LiIO\(_3\) in the far infrared}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-37905}, year = {1970}, abstract = {No abstract available}, language = {en} } @article{FrankeScheer1970, author = {Franke, Werner W. and Scheer, Ulrich}, title = {The ultrastructure of the nuclear envelope of amphibian oocytes: a reinvestigation. I. The mature oocyte}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32098}, year = {1970}, abstract = {1 n order to review the contradictory statements on the ultrast ructure of the nuclear envelope, a study was undertaken combining section and negat ive stai ning electron microscopy on manually isolated oocyte nuclei and nuclear envelopes from six amphibian species including Anura as well as Urodela. The a ppeara nce of the negatively stained iso lated nuclear envelopes is described in deta il and the dependence on the preparation co nditions used is emphas ized . Pore complex structures such as pore perimeter, central granule, an nul ar components, interna l fibrils, and annu lus-attached fibrils could be identified by both techniques, negat ive staining and sect ions. Comparative studies show that no marked diffe rences ex ist in the structural data of the nuclear envelope among the investigated amphibians and the significance of the structural components is discussed. A model of the nuclea r pore complex based on the findings of the present investigation is prese nted.}, language = {en} } @article{Becker1970, author = {Becker, Charles R.}, title = {Impurity-induced absorption in or near the phonon gap of KI}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-30757}, year = {1970}, abstract = {In or near the phonon gap of KI a single weak line due to OW (OD\(^-\)) at 69.7 (69.3) cm\(^{-1}\) has been observed. A second and much stronger line at 94.1 cm\(^{-1}\) was shown not to be related to OH\(^-\), but instead is thought to be due to CO\(_3\).}, language = {en} } @article{WeissSebaldBuecher1971, author = {Weiss, H. and Sebald, Walter and B{\"u}cher, T.}, title = {Cycloheximide resistant incorporation of amino acids into a polypeptide of the cytochrome oxidase of Neurospora crassa}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62866}, year = {1971}, abstract = {Radioaetive leueine was ineorporated by N eurospora crassa mitoehondria in vivo in the presence of cyeloheximide. When the membrane protein of these mitochondria was ehromatographieally separated on oleyl polymethaerylie aeid resin, \& nurober of fraetions were obtained whieh differ with respeet to their eontents of radioaetivity and eytoehromes. The highest speeifie radioaetivity was found in the fraction eontaining eytoehrome aa3• This fraetion proved to be a pure and enzymatically aetive cytoehrome oxidase. Its ratio of absorbanee at 280 nm (ox)/ 443 nm (red.) was 2.1. By means of sodium dodeeylsulfate gel-electrophoresis, this enzymewas separated into five polypeptides with molecular weights of 30000, 20000, 13000, 10000, and 8000. Only the polypeptide with the molecular weight 20000 displayed a high specific radioaetivity.}, subject = {Biochemie}, language = {en} } @article{ChristlReichRoberts1971, author = {Christl, Manfred and Reich, H. J. and Roberts, J. D.}, title = {Nuclear Magnetic Resonance Spectroscopy. Carbon-13 Chemical Shifts of Methylcyclopentanes, Cyclopentanols, and Cyclopentyl Acetates}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-57862}, year = {1971}, abstract = {No abstract available}, subject = {Organische Chemie}, language = {en} } @article{LutzWinklerDunitz1971, author = {Lutz, Werner K. and Winkler, F. K. and Dunitz, J. D.}, title = {Crystal structure of the antibiotic monensin similarities and differences betweeen free acid and metal complex}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-61228}, year = {1971}, abstract = {The structure of monensin, C36H620 11 , has been deterrnined by X-ray analysis of its crystalline monohydrate (orthorhombic, a = 15.15, b = 23.61, c = 10.65 A, Z = 4, space group P212121). Phases were assigned by direct methods, malring use of the 'tangent formula'. Although the conformation of the free acid resembles that of the silver salt in being cyclic, there are differences in the hydrogen bonding pattern. These featurcs are discussed in relation to the cornplexation of metal ions by m.onensin.}, subject = {Toxikologie}, language = {en} } @article{LutzFruehSimon1971, author = {Lutz, Werner K. and Fr{\"u}h, P. U. and Simon, W.}, title = {Microcalorimetric determination of ΔH0, ΔG0 and ΔS0 for the interaction of the carrier antibiotics nigericin and monensin with sodium and potassium ions}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-61218}, year = {1971}, abstract = {The thermodynainic parameters ΔH0, ΔG0 and ΔS0 - and thereby the equilibrium constants - for the complexation of the carrier antibiotics nigericin and monensin with sodium and potassium ions in methanol at 25°C have been determined by microcalorimetry. Tbc results are discussed in terms of the nature of the interaction between ligands and cations.}, subject = {Toxikologie}, language = {en} } @book{KartenbeckZentgrafScheeretal.1971, author = {Kartenbeck, J. and Zentgraf, H. and Scheer, Ulrich and Franke, Werner W.}, title = {The nuclear envelope in freeze-etching}, isbn = {3-540-05538-X}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-40534}, publisher = {Universit{\"a}t W{\"u}rzburg}, year = {1971}, abstract = {No abstract available}, subject = {Anatomie}, language = {en} } @article{FrankeKartenbeckZentgrafetal.1971, author = {Franke, Werner W. and Kartenbeck, J{\"u}rgen and Zentgraf, Hanswalter and Scheer, Ulrich and Falk, Heinz}, title = {Membrane-to-membrane cross-bridges. A means to orientation and interaction of membrane faces}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32122}, year = {1971}, abstract = {No abstract available}, language = {en} } @article{Becker1971, author = {Becker, Charles R.}, title = {Evidence for a quasi-phonon gap in CsCl}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-30767}, year = {1971}, abstract = {The observed impurity induced far-infrared absorption in CsCl : Rb\(^+\) and CsCl : K\(^+\) is compared with a calculated density of acoustic phonon states in CsCl. The absorption due to CsCl : Rb\(^+\) displays a minimum between the acoustic and optic phonon bands. A narrow line is observed in CsCl: K\(^+\) at 85.8 cm\(^{-1}\) which falls in this quasi-phonon gap.}, language = {en} } @article{FrankeScheer1971, author = {Franke, Werner W. and Scheer, Ulrich}, title = {Some structural differentiations in the HeLa cell: heavy bodies, annulate lamellae and cotte de maillet endoplasmic reticulum}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-40614}, year = {1971}, abstract = {A small fraction of HeLa cells within an exponentially growing culture showed cisternal differentiations, such as cytoplasmic as well as intranuclear annulate lamellae and special smooth surfaced endoplasmic reticulum aggregates with a typical "Cotte de maillet" appearance. Additionally, clusters of dense granules were observed in the cytoplasm which were often associated with polysomes and strongly resembled the so-called "heavy bodies" known in particular in diverse oocytes. The functional meaning of these structures is discussed. Moreover, it is deduced from the ultrastructural identity of the pore complexes in the nuclear envelope and the cytoplasmic and intranuclear annulate lamellae that the pore complex material with its highly ordered arrangement is not a structure characteristic for nucleocytoplasmically migrating material, but rather is a general structural expression of a tight binding of ribonucleoprotein (RNP) to cisternal membranes. The pore complexes are thought of as representing sites of a RNP-storage. A similar functioning is hypothesized for the "heavy body"like aggregates. To the current hypotheses on the formation of annulate lamellae and the nuclear envelope, which are based on the concept of membrane continuities and constancies, the alternative view of a self assembly mechanism of membrane constituents on nucleoprotein structures is added.}, subject = {Cytologie}, language = {en} } @article{SchwabSebaldWeiss1972, author = {Schwab, A. J. and Sebald, Walter and Weiss, H.}, title = {Different pool sizes of the precursor polypeptides of cytochrome oxidase from Neurospora crassa.}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62841}, year = {1972}, abstract = {Pulse-labelling experiments with growing Neurospora crassa revealed that the polypeptides composing the protein moiety of a cytochrome oxidase preparation are derived from at least four independent pools of precursor polypeptides. The pool sizes range from 2 ° f 0 to 25 °/0 of the amount of the corresponding polypeptide present in cytochrome oxidase. The smallest pool is assigned to a polypeptide of mitochondrial origm. Serial pools were found for one of the polypeptides.}, subject = {Biochemie}, language = {en} } @article{SebaldWeissJackl1972, author = {Sebald, Walter and Weiss, H. and Jackl, G.}, title = {Inhibition of the assembly of cytochrome oxidase in Neurospora crassa by chloramphenicol}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62852}, year = {1972}, abstract = {Cytochrome oxidasewas prepared from Neurospora crassa by chromatography on oleyl polymethacrylic acid resin and separated into seven polypeptides by polyacrylamide gel electrophoresis in the presence of sodium dodecylsulfate. Incorporation oflabelled amino acids into the single polypeptideswas investigated after a pulse labelling in the absence and presence of chloramphenicol, and afterwashing out the inhibitor. Chloramphenicol (4 mg/ml) inhibited amino acid incorporation into all polypeptides 90-95\%• while labeHing of the whole membrane protein was inhibited only 30\%• Mter washing out the inhibitor and further growth of the cells. the four smaller polypeptides were highly labelled, whereas the other polypeptides showed only a. small increase in radioactivity. It is concluded that the four small-sized polypeptides of cytochrome oxidase are synthesized but not integrated into the functional enzyme under the action of chloramphenicol.}, subject = {Biochemie}, language = {en} } @article{ChristlRoberts1972, author = {Christl, Manfred and Roberts, J. D.}, title = {Nuclear Magnetic Resonance Spectroscopy : Carbon-13 Chemical Shifts of Small Peptides as a Function of pH}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-57885}, year = {1972}, abstract = {No abstract available}, subject = {Organische Chemie}, language = {en} } @article{DeciusBeckerFredericks1972, author = {Decius, J. C. and Becker, Charles R. and Fredericks, W. J.}, title = {Force constants of the metaborate ion in alkali halides}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-50935}, year = {1972}, abstract = {No abstract available}, subject = {Physik}, language = {en} } @article{GoebelKreft1972, author = {Goebel, Werner and Kreft, J{\"u}rgen}, title = {Accumulation of replicative intermediates and catenated forms of the colicinogenic factor E\(_1\) in E. coli during the replication at elevated temperatures}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-60625}, year = {1972}, abstract = {No abstract available}, subject = {Biologie}, language = {en} } @article{ChristlRoberts1972, author = {Christl, Manfred and Roberts, John D.}, title = {Carbon-13 Nuclear Magnetic Resonance Spectroscopy : Conformational Analysis of Methyl-Substituted Cycloheptanes, Cycloheptanols, and Cycloheptanones}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-41663}, year = {1972}, abstract = {The 130 chemical shifts were determined of the carbons in 12 cycloheptanes, 21 cycloheptanols, and 8 cycloheptanones. In some cyc1oheptanols and cyc1oheptanones, the assignments have been obtained unambiguously by the synthesis of deuterated derivatives and the use of paramagnetic-shift reagents. Substituent effects for the different types of groups have been calculated. The most informative data about the cyc10heptane conformations were provided by the relatively well understood I' effects. The results are generally in,good agreement with predictions based on the twist-chair form, which has been predicted by Hendrickson to be the most stable conformation. Pairs of cis-trans isomers are found to have rather characteristic differences in their 130 spectra. This fact was used to assign the resonances found for cis-trans mixtures of methyl-substituted cyc1oheptanols to specific isomers.}, language = {en} } @article{FrankeScheerFritsch1972, author = {Franke, Werner W. and Scheer, Ulrich and Fritsch, Hansj{\"o}rg}, title = {Intranuclear and cytoplasmic annulate lamellae in plant cells}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32148}, year = {1972}, abstract = {No abstract available}, language = {en} } @article{ScheerFranke1972, author = {Scheer, Ulrich and Franke, Werner W.}, title = {Annulate lamellae in plant cells: formation during microsporogenesis and pollen development in Canna generalis Bailey}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32160}, year = {1972}, abstract = {The occurrence of stacked annulate tamellae is documented for a plant cell system, namely for pollen mother cells and developing pollen grains of Canna generalis. Their structural subarchiteeture and relationship to endoplasmie reticulum (ER) and nuclear envelope cisternae is described in detail. The results demonstrate structural homology between plant and animal annulate lamellae and are compatible with, though do not prove, the view that annulate lamcllar cisternae may originate as a degenerative form of endoplasmic retieulum.}, language = {en} } @article{FrankeScheer1972, author = {Franke, Werner W. and Scheer, Ulrich}, title = {Structural details of dictyosomal pores}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32155}, year = {1972}, abstract = {Structural details of the dictyosomal pores in several plant cell types are described from tangential and cross sections of Golgi cisternae. Frequency distributions of the sizes of such Golgi pores are given and compared with the corresponding values of nuclear pores in the same cells. Golgi pore inner diameters are less homogeneously distributed and can be as small as 100 A or less. They are not simply cisterna I holes, but are often associated with centrally located electron dense granules or rods and with inner pore filaments. This organization, which is very common in dictyosomal pores in plant and animal cells, has some similarities with the structural architecture of nuclear envelope and annulate lamellar pore complexes. The particulate material associated with the dictyosomal pores shows spatial and structural relationship to cytoplasmic ribosomes. Possible modes of Golgi pore formation and some consequences of these observations for interpretation of nuclear pore structures are discussed.}, language = {en} } @article{FrankeKartenbeckKrienetal.1972, author = {Franke, Werner W. and Kartenbeck, J{\"u}rgen and Krien, S. and VanderWoude, W. J. and Scheer, Ulrich and Morr{\´e}, D. J.}, title = {Inter- and intracisternal elements of the Golgi apparatus: A system of membrane-to-membrane cross-links}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-39514}, year = {1972}, abstract = {Electron opaque cross-bridge structures span the inter- and intracisternal spaces and provide membrane-to-membrane connections between adjacent cisternae of dictyosomes of pollen tubes of Clivia and Lilium. Additionally, the classic intercisternal rods, characteristic of intercisternal regions near the maturing face of dictyosomes, are connected with the adjacent membranes through similar cross-bridge elements. We suggest that these structural links are responsible for maintaining the flattened appearance of the central parts of Golgi apparatus cisternae as well as for the coherence of cisternae within the stack. Observations on other plant (e.g. microsporocytes of Canna) and animal cells (e.g. rodent liver and hepatoma cells, newt spermatocytes) show that such an array of membrane cross-links is a universal feature of Golgi apparatus architecture. The cross-bridges appear as part of the complex "zone of exclusion" which surrounds dictyosomes, entire Golgi apparatus and Golgi apparatus equivalents in a variety of cell types.}, language = {en} } @article{Scheer1972, author = {Scheer, Ulrich}, title = {The ultrastructure of the nuclear envelope of amphibian ooctyes: IV. On the chemical nature of the nuclear pore complex material}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-39500}, year = {1972}, abstract = {In order to investigate the chemical composition of the nuclear pore complexes isolated nuclei from mature Xenopus laevis oocytes were manually fractioned into nucleo· plasmic aggregates and the nuclear envelopes. The whole isolation procedure takes no more than 60- 90 sec, and the pore complexes of the isolated envelopes are well preserved as demonstrated by electron microscopy. Minor nucleoplasmic and cytoplasmic contaminations associated with the isolated nuclear envelopes were determined with electron microscopic morphometry and were found to be quantitatively negligible as far as their mass and nucleic acid content is concerned. The RNA content of the fractions was determined by direct phosphorus analysis after differential alkaline hydrolysis. Approximately 9\% of the total nuclear RNA of the mature Xenopus egg was found to be attached to the nuclear envelope. The nonmembranous elements of one pore complex contain 0.41 X 10- 16 g RNA. This value agrees well with the content estimated from morphometric data. The RNA package density in the pore complexes (270 X 10- 15 g/fJ-3) is compared with the nucleolar, nucleoplasmic and cytoplasmic RNA concentration and is discussed in context with the importance of the pore complexes for the nucleo-cytoplasmic transport of RNA-containing macromolecules. Additionally, the results of the chemical analyses as well as of the 3H-actinomycin D autoradiography and of the nucleoprotein staining method of Bernhard (1969) speak against the occurence of considerable amounts of DNA in the nuclear pore complex structures.}, language = {en} } @article{BeckerMartin1972, author = {Becker, Charles R. and Martin, T. P.}, title = {Infrared absorption by Impurity-pair resonant modes in NaCl:F}, isbn = {1098-0121}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-37840}, year = {1972}, abstract = {New resonant-mode infrared absorption lines have been observed in NaCl with high concentrations of fluorine impurities. The quadratic concentration dependence of the strength of these lines indicates that they are due to pairs of fluorine impurities. At the resonant frequencies, the motion of some host ions appears to be as important as the motion of the impurities themselves.}, subject = {Festk{\"o}rperphysik}, language = {en} } @misc{IshigamaBeckerMartinetal.1972, author = {Ishigama, M. and Becker, Charles R. and Martin, T. P. and Prettl, W.}, title = {Impurity-pair mode in NaCl:KF}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-31255}, year = {1972}, abstract = {No abstract available}, language = {en} } @article{Linsenmair1972, author = {Linsenmair, Karl Eduard}, title = {Anemomenotactic orientation in beetles and scorpions}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-78118}, year = {1972}, abstract = {Scorpions, living in North African semideserts are - in spite of disrupting experimental interferences - able to maintain a certain direction in their natural environment in the dark on a plane surface. Under comparable laboratory conditions, excluding the possibility of light or gravity orientation, they can orient themselves if a directed air current passes over the "arena." In most cases the scorpions do not run necessarily with or against the wind, but rather maintain constant angles to the air current for anywhere from minutes to many hours. They are running anemomenotactically (ref. 1). Under identical conditions many species of beetles also orient themselves to air currents (refs. 2 to 4). The main problems to be solved in the study of anemomenotactic orientation are: (1) Which physical qualities of the air current have an influence on the anemomenotaxis? (2) With which sense organs do beetles and scorpions perceive wind directions? (3) Which physiological mechanism is the basis of anemomenotactic orientation? (4) What is the biological significance of anemomenotaxis in beetles and scorpions? With respect to these problems, more study has been done on beetles than on scorpions. Therefore, due to lack of space, I shall discuss mainly some of the results obtained in experiments with dung beetles (Geotrupes silvaticus, G. ,Stercorarius, G. armifrons, G. niger, Scarabaeus variolosus) and tenebrionid beetles (Tenebrio molitor, Pimelia grossa, P. tenuicomis, Scaurus dubius).}, subject = {Biologie}, language = {en} } @article{EckertFrankeScheer1972, author = {Eckert, W. A. and Franke, Werner W. and Scheer, Ulrich}, title = {Actinomycin D and the central granules in the nuclear pore complex: thin sectioning versus negative staining}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-40636}, year = {1972}, abstract = {Thin section electron microscopy of Actinomycin D treated Tetrahymena cells and amphibian oocytes (Xenopus laevis, Triturus aZpestris) reveal no reduction in the central granules in the nuclear pore complexes. Possible reasons for the diversity between these results and earlier observations using negatively stained isolated nuclear envelopes from the same objects are discussed. The results clearly show that the presence of central granules within the nuclear pores does neither depend on nuclear RNA synthesis nor does indicate nucleocytoplasmic RNA transport. This conclusion leads to a reconsideration of the nature of the central granule. The functioning of the central granule of the nuclear pore complexes is further discussed in connection with recent studies on the ultrastructure of various types of cisternal pores.}, language = {en} } @article{SebaldMachleidtOtto1973, author = {Sebald, Walter and Machleidt, W. and Otto, J.}, title = {Products of mitochondrial protein synthesis in Neurospora crassa. Determination of equimolar amounts of three products in cytochrome oxidase on the basis of amino-acid analysis}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62827}, year = {1973}, abstract = {Cytochrome oxidase isolated from N eurospora crassa was resolved into seven protein eomponents by eleetrophoresis in polyaerylamide gels eontaining sodium dodeeylsulfate. The apparent molecular weights were determined tobe 41000, 28500, 21000, 16000, 14000, 11500 and 10000 for the eomponents 1, 2, 3, 4, 5, 6, and 7, respectively. The components 1, 2 and 3 are synthesized on mitochondrial ribosomes as shown by the incorporation of radioactive amino aeids in the presenee of cyeloheximide. Amino-acidanalysis of the isolated components 1, 2 and 3 revealed a high content of apolar amino acids and a low eontent of basic amino aeids compared to an average amino-aeid eomposition of components 4-7. Components 1, 2 and 3 eontribute 27.9°/0, 18°/0 and 14.2°/0 to the whole eytoehrome oxidase protein. This was calculated from the contributions of the single eomponents to the totalleueine eontent of the enzyme and the leueine eontents (nmol leueine per mg protein) of the single eomponents as determined by amino-aeid analysis. Equimolar relations of the components 1, 2 and 3 are found by dividing the amounts of protein by their apparent molecular weights. A stoichiometry of 1:1:1 results assuming a minimal molecular weight of 150000 for the whole cytochrome oxidase protein. On the basis of the heme a content a molecular weight of about 70000 per heme group was determined, using an absorption coeffieient L1e605 (redueed minus oxidized) of 12 mM-1 cm-1• It is concluded that the smallest structural unit of eytochrome oxidase contains two heme groups.}, subject = {Biochemie}, language = {en} } @article{WeissSebaldSchwabetal.1973, author = {Weiss, H. and Sebald, Walter and Schwab, A. J. and Kleinow, W. and Lorenz, B.}, title = {Contribution of mitochondrial and cytoplasmic protein synthesis to the formation of cytochrome b and cytochrome aa\(_3\)}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62835}, year = {1973}, abstract = {A cytochrome b preparation from Neurospora crassa mitochondria is found to consist of three polypeptides (apparent molecular weight 10 000, 11 000 and 32 000), a cytochrome aa3 preparation of six to seven polypeptides (apparent molecular weight 8 000, 11 000, 13 000, 18 000, 28 000 and 36 000). Selective incorporation of radioactive amino acids by eilher mitochondrial protein synthesis when the cytoplasmic one is blocked or by the cytoplasmic protein synthesis, when the mitochondrial one is blocked, indicates that one cytochrome b polypeptide (mw 32 000) and one to three cytochrome aa3 polypeptides (mw 36 000, 28 000 and 18 000) are mitochondrial translation products, the other cytochrome b and cytochrome aa3 polypeptides cytoplasmic translation products. The delayed appearance of labeling in the cytochrome b and cytochrome aa3 polypeptides compared to the average cell protein after a pulse of <~H leueine revealed that these polypeptides are derived from separate pools of precursor polypeptides. The pool sizes range from 2 p. cent to 25 p. cent of the amount of the corresponding polypeptide present in the cytochromes. The 32 000 molecular weight polypeptide of cytochrome band at least the 18 000 molecular weight polypeptide of cytochrome aa\(_3\) are mitochondrial translation products as well in the fungus Neurospora crassa as in the insect Locusta migratoria. So, despite the fact that the size of mitochondrial DNA and mitochondrial ribosomes is reduced in insects, the products have maintained their characteristics.}, subject = {Biochemie}, language = {en} } @article{ChristlWarrenHawkinsetal.1973, author = {Christl, Manfred and Warren, J. D. and Hawkins, B. L. and Roberts, J. D.}, title = {\(^{13}\)C and \(^{15}\)N Nuclear Magnetic Resonance Spectroscopy of Nitrile Oxides and Related Reaction Products : Unexpected \(^{13}\)C and \(^{15}\)N Nuclear Magnetic Resonance Parameters of 2,4,6-Trimethylbenzonitrile Oxide}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-57894}, year = {1973}, abstract = {No abstract available}, subject = {Organische Chemie}, language = {en} } @article{DerksenTrendelenburgScheeretal.1973, author = {Derksen, J. and Trendelenburg, Michael F. and Scheer, Ulrich and Franke, Werner W.}, title = {Spread chromosomal nucleoli of Chironomus salivary glands}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32209}, year = {1973}, abstract = {No abstract available}, language = {en} } @article{FrankeTrendelenburgScheer1973, author = {Franke, Werner W. and Trendelenburg, Michael F. and Scheer, Ulrich}, title = {Natural segregation of nucleolar components in the course of plant cell differentiation}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32182}, year = {1973}, abstract = {Segregation of the nucleolar components is described in the differentiated nucleus of the generative cell in the growing Clivia and Lilium pollen tubes. This finding of a natural nucleolar segregation is discussed against the background of current views of the correlations of nucleolar morphology and transcriptional activity.}, language = {en} } @article{ScheerTrendelenburgFranke1973, author = {Scheer, Ulrich and Trendelenburg, Michael F. and Franke, Werner W.}, title = {Transcription of ribosomal RNA cistrons: Correlation of morphological and biochemical data}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32195}, year = {1973}, abstract = {Electron microscopic spread preparations of oocyte nucleoli (lampbrush stage) of various amphibians are quantitatively evaluated and the length distributions of repeat-, matrix-, and spacer-units along the rRNA cistron containing axes are given. The correlation of the matrix unit data with the gel electrophoretic pattern of labelled nuclear RNA from the same oocytes is examined. The mean value of the matrix unit corresponds fairly well to a 2.6 million D peak of pre-rRNA but the distribution of both matrix units and labelled pre-rRNAs shows an asymmetrical heterogeneity indicating the existence of some larger primary transcription products of rDNA. Novel structural aspects are described in the spacer regions which suggest that transcription does also take place in DNP regions between the matrix units. A special "prelude piece" coding for approx. 0.5 million D of RNA is frequently visualized in the spacer segments at the beginning of a matrix unit. Possible artifacts resulting from the preparation, the relative congruence between the data obtained using both methods, and the functional meaning of the findings are discussed against the background of current concepts of structural organization and transcription products of nucleolar DNA.}, language = {en} } @article{Scheer1973, author = {Scheer, Ulrich}, title = {Nuclear pore flow rate of ribosomal RNA and chain growth rate of its precursor during oogenesis of Xenopus laevis}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32178}, year = {1973}, abstract = {The number of ribosomal RNA molecules which are transferred through an average nuclear pore complex per minute into the cytoplasm (nuclear pore flow rate, NPFR) during oocyte growth of Xenopus laevis is estimated. The NPFR calculations are based on determinations of the increase of cytoplasmic rRNA content during defined time intervals and of the total number of pore complexes in the respective oogenesis stages. In the mid-la mpbrush stage (500:"700 I'm oocyte diameter) the NPFR is maximal with 2.62 rRNA molecules/ pore/ minute. Then it decreases to zero at the end of oogenesis. The nucleocytoplasmic RNA f10w rates determined are compared with corresponding values of other cell types. The molecular weight of the rRNA precursor transcribed in the extrachromosomal nucleoli of Xenopus lampbrush stage oocytes is determined by acrylamide gel electrophoresis to be 2.5 x 10· daltons. From the temporal increase of cytoplasmic rRNA (3.8 I'g per oocyte in 38 days) and the known number of simultaneously growing precursor molecules in the nucleus the chain growth rate of the 40 S precursor RNA is estimated to be 34 nucleotides per second.}, language = {en} } @article{TrendelenburgScheerFranke1973, author = {Trendelenburg, Michael F. and Scheer, Ulrich and Franke, W. W.}, title = {Structural organization of the transcription of ribosomal DNA in oocytes of the house cricket}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-33113}, year = {1973}, abstract = {No abstract available}, language = {en} } @misc{DeciusBeckerFredericks1973, author = {Decius, J. C. and Becker, Charles R. and Fredericks, W. J.}, title = {Erratum: Force constants of the metaborate ion in alkali halides}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-37992}, year = {1973}, abstract = {Erratum for the article: Decius, J. C. ; Becker, Charles R. ; Fredericks, W. J. : Force constants of the metaborate ion in alkali halides, In: THE JOURNAL OF CHEMICAL PHYSICS (1972), 56, 10, 5189-5190.}, language = {en} } @article{BruentrupChristl1973, author = {Br{\"u}ntrup, Gisela and Christl, Manfred}, title = {Darstellung und Thermolyse der Hexamethyldewarbenzol-Cycloaddukte aromatischer Nitriloxide und des Diphenylnitrilimins}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-30257}, year = {1973}, abstract = {No abstract available}, language = {en} } @misc{UnkelbachBeckerKoehleretal.1973, author = {Unkelbach, K. H. and Becker, Charles R. and K{\"o}hler, H. and Middendorff, A. V.}, title = {Optical Phonons of Bi\(_2\)Te\(_3\)}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-30809}, year = {1973}, abstract = {No abstract available}, language = {en} } @article{FrankeZentgrafScheer1973, author = {Franke, Werner W. and Zentgraf, Hanswalter and Scheer, Ulrich}, title = {Membrane linkages at the nuclear envelope}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-40596}, year = {1973}, abstract = {Electron-opaque material is shown in the perinuclear cisternae of various cell types to connect the inner and outer nuclear membrane faces. Similar bridges were observed between the outer nuclear membrane and the outer mitochondrial membrane. The intracisternal bridges of the nuclear envelope appear to be important for the structural stability of the perinuclear cisterna. Stable structural linkage of mitochondria to the outer nuclear membrane might be relevant to the understanding of the characteristic juxtanuclear accumulation of mitochondria and also provide arguments for the discussions of certain biochemical activities found in nuclear and nuclear membrane fractions.}, subject = {Cytologie}, language = {en} } @incollection{CranachEllgring1973, author = {Cranach, Mario von and Ellgring, Johann Heinrich}, title = {Problems in the recognition of gaze direction}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-42147}, publisher = {Universit{\"a}t W{\"u}rzburg}, year = {1973}, abstract = {No abstract available}, language = {en} } @article{ChristlBruentrup1974, author = {Christl, Manfred and Br{\"u}ntrup, Gisela}, title = {Diimine Reduction and Ozonolysis of Benzvalene}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-57953}, year = {1974}, abstract = {Via reduction of benzvalene (1) with diirnine tricyclo[3.1.0.02•6]hexane is obtained in good yield. The procedure renders 3, which has already been synthesized by Lemal and Shim, accessible much easier and in larger quantities. IH and 13C n.m.r. spectroscopic data are discussed. Both the thermal and the AgBF4-catalyzed rearrangernent of 3 yield 1,3-cyclohexadiene (8). - The ozonolysis of 1 with subsequent LiAIH4-reduction results in cis-I,3- bis(hydroxyrnethyl)cyclobutane (13a).}, subject = {Organische Chemie}, language = {en} } @article{KreftGoebel1974, author = {Kreft, J{\"u}rgen and Goebel, Werner}, title = {Complex Co1E1 DNA in Escherichia coli and Proteus mirabilis}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-47044}, year = {1974}, abstract = {Incubation of the colicinogenic Escherichia coli strain JC 411 (ColE1) at elevated temperatures (47-49°) leads to the accumulation of catenated molecules and replicative intermediates of this plasmid. Mature supercoiled OolE1 DNA molecules synthesized under these conditions have an increased number of tertiary turns as shown by electron microscopy. The monomeric tightly supercoiled molecules possess a slightly slower sedimentation rate and a higher binding capacity for ethidium bromide than supercoiJed monomers synthesized at lower temperatures. Recombination deficient mutants of E. coli recA, recB and recC, which carry the ColE1 plasmid, form about the same amount of catenated molecules at the elevated temperature as a rec+ strain. In addition, we have observed by electron microscopy a small percentage (.--.5\% of the circular DNA molecules) of minicircular DNA molecules in all preparations of JC 411 (CoIE1). They are homogenous in size, with a molecular weight of 1.4 X 106 daltons. Addition of chloramphenicol to a culture of Proteus mirabilis (ColE1) leads to an increased amount of higher multiple circular oligomers and to a stimulated accumulation of catenated OolE1 DNA molecules of varying sizes. ColE1 DNA synthesis is more thermosensitive than chromosomal DNA replication in P. mirabili8. Plasmid replication stops completely at temperatures above 43°C.}, language = {en} }