@article{HornKellerHildebrandtetal.2016,
  author    = {Horn, Hannes and Keller, Alexander and Hildebrandt, Ulrich and K{\"a}mpfer, Peter and Riederer, Markus and Hentschel, Ute},
  title     = {Draft genome of the \(Arabidopsis\) \(thaliana\) phyllosphere bacterium, \(Williamsia\) sp. ARP1},
  series = {Standards in Genomic Sciences},
  volume    = {11},
  journal   = {Standards in Genomic Sciences},
  number    = {8},
  doi       = {10.1186/s40793-015-0122-x},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-146008},
  year      = {2016},
  abstract  = {The Gram-positive actinomycete \(Williamsia\) sp. ARP1 was originally isolated from the \(Arabidopsis\) \(thaliana\) phyllosphere. Here we describe the general physiological features of this microorganism together with the draft genome sequence and annotation. The 4,745,080 bp long genome contains 4434 protein-coding genes and 70 RNA genes. To our knowledge, this is only the second reported genome from the genus \(Williamsia\) and the first sequenced strain from the phyllosphere. The presented genomic information is interpreted in the context of an adaptation to the phyllosphere habitat.},
  language  = {en}
}
@article{HornHentschelRamadanAbdelmohsen2015,
  author    = {Horn, Hannes and Hentschel, Ute and Ramadan Abdelmohsen, Usama},
  title     = {Mining Genomes of Three Marine Sponge-Associated Actinobacterial Isolates for Secondary Metabolism},
  series = {Genome Announcements},
  volume    = {3},
  journal   = {Genome Announcements},
  number    = {5},
  doi       = {10.1128/genomeA.01106-15},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-124887},
  pages     = {e01106-15},
  year      = {2015},
  abstract  = {Here, we report the draft genome sequences of three actinobacterial isolates, Micromonospora sp. RV43, Rubrobacter sp. RV113, and Nocardiopsis sp. RV163 that had previously been isolated from Mediterranean sponges. The draft genomes were analyzed for the presence of gene clusters indicative of secondary metabolism using antiSMASH 3.0 and NapDos pipelines. Our findings demonstrated the chemical richness of sponge-associated actinomycetes and the efficacy of genome mining in exploring the genomic potential of sponge-derived actinomycetes.},
  language  = {en}
}
@article{HarjesRyuAbdelmohsenetal.2014,
  author    = {Harjes, Janno and Ryu, Taewoo and Abdelmohsen, Usama Ramadan and Moitinho-Silva, Lucas and Horn, Hannes and Ravasi, Timothy and Hentschel, Ute},
  title     = {Draft Genome Sequence of the Antitrypanosomally Active Sponge-Associated Bacterium Actinokineospora sp. Strain EG49},
  doi       = {10.1128/genomeA.00160-14},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-112776},
  year      = {2014},
  abstract  = {The marine sponge-associated bacterium Actinokineospora sp. strain EG49 produces the antitrypanosomal angucycline-like compound actinosporin A. The draft genome of Actinokineospora sp. EG49 has a size of 7.5 megabases and a GC content of 72.8\% and contains 6,629 protein-coding sequences (CDS). antiSMASH predicted 996 genes residing in 36 secondary metabolite gene clusters.},
  subject      = {Strahlenpilze},
  language  = {en}
}
@article{ChengMacIntyreRamadanAbdelmohsenetal.2015,
  author    = {Cheng, Cheng and MacIntyre, Lynsey and Ramadan Abdelmohsen, Usama and Horn, Hannes and Polymenakou, Paraskevi N. and Edrada-Ebel, RuAngelie and Hentschel, Ute},
  title     = {Biodiversity, Anti-Trypanosomal Activity Screening, and Metabolomic Profiling of Actinomycetes Isolated from Mediterranean Sponges},
  series = {PLoS One},
  volume    = {10},
  journal   = {PLoS One},
  number    = {9},
  doi       = {10.1371/journal.pone.0138528},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-125138},
  pages     = {e0138528},
  year      = {2015},
  abstract  = {Marine sponge-associated actinomycetes are considered as promising sources for the discovery of novel biologically active compounds. In the present study, a total of 64 actinomycetes were isolated from 12 different marine sponge species that had been collected offshore the islands of Milos and Crete, Greece, eastern Mediterranean. The isolates were affiliated to 23 genera representing 8 different suborders based on nearly full length 16S rRNA gene sequencing. Four putatively novel species belonging to genera Geodermatophilus, Microlunatus, Rhodococcus and Actinomycetospora were identified based on a 16S rRNA gene sequence similarity of < 98.5\% to currently described strains. Eight actinomycete isolates showed bioactivities against Trypanosma brucei brucei TC221 with half maximal inhibitory concentration (IC50) values <20 μg/mL. Thirty four isolates from the Milos collection and 12 isolates from the Crete collection were subjected to metabolomic analysis using high resolution LC-MS and NMR for dereplication purposes. Two isolates belonging to the genera Streptomyces (SBT348) and Micromonospora (SBT687) were prioritized based on their distinct chemistry profiles as well as their anti-trypanosomal activities. These findings demonstrated the feasibility and efficacy of utilizing metabolomics tools to prioritize chemically unique strains from microorganism collections and further highlight sponges as rich source for novel and bioactive actinomycetes.},
  language  = {en}
}
@article{AbdelmohsenYangHornetal.2014,
  author    = {Abdelmohsen, Usama Ramadan and Yang, Chen and Horn, Hannes and Hajjar, Dina and Ravasi, Timothy and Hentschel, Ute},
  title     = {Actinomycetes from Red Sea Sponges: Sources for Chemical and Phylogenetic Diversity},
  doi       = {10.3390/md12052771},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-112882},
  year      = {2014},
  abstract  = {The diversity of actinomycetes associated with marine sponges collected off Fsar Reef (Saudi Arabia) was investigated in the present study. Forty-seven actinomycetes were cultivated and phylogenetically identified based on 16S rRNA gene sequencing and were assigned to 10 different actinomycete genera. Eight putatively novel species belonging to genera Kocuria, Mycobacterium, Nocardia, and Rhodococcus were identified based on sequence similarity values below 98.2\% to other 16S rRNA gene sequences available in the NCBI database. PCR-based screening for biosynthetic genes including type I and type II polyketide synthases (PKS-I, PKS-II) as well as nonribosomal peptide synthetases (NRPS) showed that 20 actinomycete isolates encoded each at least one type of biosynthetic gene. The organic extracts of nine isolates displayed bioactivity against at least one of the test pathogens, which were Gram-positive and Gram-negative bacteria, fungi, human parasites, as well as in a West Nile Virus protease enzymatic assay. These results emphasize that marine sponges are a prolific resource for novel bioactive actinomycetes with potential for drug discovery.},
  subject      = {Meeresschw{\"a}mme},
  language  = {en}
}