@article{KreftFunkeHaasetal.1989, author = {Kreft, J{\"u}rgen and Funke, Dorothee and Haas, Albert and Lottspeich, Friedrich and Goebel, Werner}, title = {Production, purification and characterization of hemolysins from Listeria ivanovii and Listeria monocytogenes Sv4b.}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-60545}, year = {1989}, abstract = {In culture supematants of both Listeria ivanovii and Listeria monocytogenes Sv4b, for the first time a hemolysin of molecular weight 58 kDa was identified, which had all the characteristics of an SH-activated cytolysin, and which was therefore identified as Iisteriolysin 0 (LLO). In the case of L. ivanovii a second major supematant protein of molecular weight 24 kDa co-purified with LLO. However, the function of this protein has to be determined. In culture supematants of L. ivanovii a sphingomyelinase and a Iecithinase activity could be detected, both enzymatic activities together contributing to the pronounced hemolysis caused by L. ivanovii. The N-tenninal amino acid sequences of LLO and the 24 kDa from L. ivanovii are shown.}, subject = {Biologie}, language = {en} }