@article{KlampCampsNietoetal.2013,
  author    = {Klamp, Tobias and Camps, Marta and Nieto, Benjamin and Guasch, Francesc and Ranasinghe, Rohan T. and Wiedemann, Jens and Petr{\´a}šek, Zdeněk and Schwille, Petra and Klenerman, David and Sauer, Markus},
  title     = {Highly Rapid Amplification-Free and Quantitative DNA Imaging Assay},
  series = {Scientific Reports},
  volume    = {3},
  journal   = {Scientific Reports},
  number    = {1852},
  doi       = {10.1038/srep01852},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-130500},
  year      = {2013},
  abstract  = {There is an urgent need for rapid and highly sensitive detection of pathogen-derivedDNAin a point-of-care (POC) device for diagnostics in hospitals and clinics. This device needs to work in a 'sample-in-result-out' mode with minimum number of steps so that it can be completely integrated into a cheap and simple instrument. We have developed a method that directly detects unamplified DNA, and demonstrate its sensitivity on realistically sized 5 kbp targetDNA fragments of Micrococcus luteus in small sample volumes of 20 mL. The assay consists of capturing and accumulating of target DNA on magnetic beads with specific capture oligonucleotides, hybridization of complementary fluorescently labeled detection oligonucleotides, and fluorescence imaging on a miniaturized wide-field fluorescence microscope. Our simple method delivers results in less than 20 minutes with a limit of detection (LOD) of,5 pMand a linear detection range spanning three orders of magnitude.},
  language  = {en}
}