@article{SebaldBuecherOlbrichetal.1968, author = {Sebald, Walter and B{\"u}cher, T. and Olbrich, B. and Kaudewitz, F.}, title = {Electrophoretic pattern of and amino acid incorporation in vitro into the insoluble mitochondrial protein of neurospora crassa wild type and mi-1 mutant}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62926}, year = {1968}, abstract = {No abstract available}, subject = {Biochemie}, language = {en} } @article{NeupertSebaldSchwabetal.1969, author = {Neupert, W. and Sebald, Walter and Schwab, A. J. and Pfaller, A. and B{\"u}cher, T.}, title = {Puromycin sensitivity of ribosomal label after incorporation of \(^{14}\)C-labelled amino acids into isolated mitochondria from Neurospora crassa}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62899}, year = {1969}, abstract = {Radioactive amino acids were incorporated into isolated mitochondria from Neurospora crassa. Then the mitochondrial ribosomes were isolated and submitted to density gradient centrifugation. A preferential labelling of polysomes was observed. However, when the mitochondrial suspension was treated with puromycin after amino acid incorporation, no radioactivity could be detected in either the monosomes or the polysomes. The conclusion is drawn that isolated mitochondria under these conditions do not incorporate significant amounts of amino acids into proteins of their ribosomes.}, subject = {Biochemie}, language = {en} } @article{SebaldSchwabBuecher1969, author = {Sebald, Walter and Schwab, A. J. and B{\"u}cher, T.}, title = {Cycloheximide resistant amino acid incorporation into mitochondrial protein from Neurospora crassa in vivo}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62900}, year = {1969}, abstract = {No abstract available}, subject = {Biochemie}, language = {en} } @article{SebaldHofstoetterHackeretal.1969, author = {Sebald, Walter and Hofst{\"o}tter, T. and Hacker, D. and B{\"u}cher, T.}, title = {Incorporation of amino acids into mitochondrial protein of the flight muscle of Locusta migratoria in vitro and in vivo in the presence of cycloheximide}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62919}, year = {1969}, abstract = {No abstract available}, subject = {Biochemie}, language = {en} } @article{NeupertSebaldSchwabetal.1969, author = {Neupert, W. and Sebald, Walter and Schwab, A. J. and Massinger, P. and B{\"u}cher, T.}, title = {Incorporation in vivo of \(^{14}\)C-labelled amino acids into the proteins of mitochondrial ribosomes from Neurospora crassa sensitive to cycloheximide and insensitive to Chloramphenicol}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62884}, year = {1969}, abstract = {Radioactive amino acids were incorporated in vivo into N eurospora crassa cells, and the mitochondrial ribosomes were isolated. The incorporation of radioactivity into the proteins of these ribosomes was inhibited by cycloheximide, but not by chloramphenicol. It is therefore concluded that these proteins are synthesized on the cycloheximide sensitive and chloramphenicol insensitive cytoplasmic ribosomes.}, subject = {Biochemie}, language = {en} } @article{ScheerFranke1969, author = {Scheer, Ulrich and Franke, Werner W.}, title = {Negative staining and adenosine triphosphatase activity of annulate lamellae of newt oocytes}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32087}, year = {1969}, abstract = {Semi -iso la ted annul a te lamellae were prepared from single newt oocy tes (Triturus alpestris) by a modified Call a n-T omlin technique. Such preparations were examined with the electron mi croscope, and the negative sta ining a ppearance of th e a nnulate lamellae is described . The annul a te lamellae can be de tected either adhering to the nuclear envelope or being detached from it. Sometimes they a re obse rved to be connected with slender tubular-like structures interpreted as pa rts of the endoplasmic reti culum. The results obta ined from negativ e sta ining a re combined with those from sections. Especially, the structural data on th e a nnula te lamellae and the nuclear envelope of the very same cell were compa red . Evidence is presented th a t in the oocytes studied the two kinds of porous cisternae, n amely a nnul a te lamellae and nuclear envelope, a re markedly distinguished in that the annul a te lamellae ex hibit a much higher pore frequency (generally about twice tha t found for the corresponding nuclear envelope) and have al so a rela tive pore area occupying as much as 32 \% to 55 \% of th e cistern al surface (compa red with 13 \% to 22 \% in the nuclear envelopes). T he pore di ame ter a nd all other ultras tructural details of the pore complexes, however, a re equi valent in both kinds of porous cisternae. Like the annuli of the nuclear pore complexes of various a nimal and pl ant cells, the a nnuli of the a nnula te lamellae pores reveal al so an eightfold symmetry of their subunits in negatively stained as well as in ectioned ma teria l. Furthermore, th e a nnul a te lamellae a re shown to be a site of activity of the Mg-Na-Kstimul a ted ATPase.}, language = {en} } @article{SebaldNeupertBirkmayer1970, author = {Sebald, Walter and Neupert, W. and Birkmayer, G. D.}, title = {Internal and external contributions to the biogenesis of mitochondrial proteins}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62876}, year = {1970}, abstract = {No abstract available}, subject = {Biochemie}, language = {en} } @article{Scheer1970, author = {Scheer, Ulrich}, title = {The ultrastructure of the nuclear envelope of amphibian oocytes: a reinvestigation. III. Actinomycin D-induced decrease in central granules within the pores.}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32110}, year = {1970}, abstract = {No abstract available}, language = {en} } @article{FrankeScheer1970, author = {Franke, Werner W. and Scheer, Ulrich}, title = {The ultrastructure of the nuclear envelope of amphibian oocytes: a reinvestigation. II. The immature oocyte and dynamic aspects}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32102}, year = {1970}, abstract = {No abstract available}, language = {en} } @article{FrankeScheer1970, author = {Franke, Werner W. and Scheer, Ulrich}, title = {The ultrastructure of the nuclear envelope of amphibian oocytes: a reinvestigation. I. The mature oocyte}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32098}, year = {1970}, abstract = {1 n order to review the contradictory statements on the ultrast ructure of the nuclear envelope, a study was undertaken combining section and negat ive stai ning electron microscopy on manually isolated oocyte nuclei and nuclear envelopes from six amphibian species including Anura as well as Urodela. The a ppeara nce of the negatively stained iso lated nuclear envelopes is described in deta il and the dependence on the preparation co nditions used is emphas ized . Pore complex structures such as pore perimeter, central granule, an nul ar components, interna l fibrils, and annu lus-attached fibrils could be identified by both techniques, negat ive staining and sect ions. Comparative studies show that no marked diffe rences ex ist in the structural data of the nuclear envelope among the investigated amphibians and the significance of the structural components is discussed. A model of the nuclea r pore complex based on the findings of the present investigation is prese nted.}, language = {en} }