@article{GrimmKleinKopeketal.2016,
  author    = {Grimm, Jonathan B. and Klein, Teresa and Kopek, Benjamin G. and Shtengel, Gleb and Hess, Harald F. and Sauer, Markus and Lavis, Luke D.},
  title     = {Synthesis of a far-red photoactivatable silicon-containing rhodamine for super-resolution microscopy},
  series = {Angewandte Chemie International Edition},
  volume    = {55},
  journal   = {Angewandte Chemie International Edition},
  number    = {5},
  doi       = {10.1002/anie.201509649},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-191069},
  pages     = {1723-1727},
  year      = {2016},
  abstract  = {The rhodamine system is a flexible framework for building small-molecule fluorescent probes. Changing N-substitution patterns and replacing the xanthene oxygen with a dimethylsilicon moiety can shift the absorption and fluorescence emission maxima of rhodamine dyes to longer wavelengths. Acylation of the rhodamine nitrogen atoms forces the molecule to adopt a nonfluorescent lactone form, providing a convenient method to make fluorogenic compounds. Herein, we take advantage of all of these structural manipulations and describe a novel photoactivatable fluorophore based on a Si-containing analogue of Q-rhodamine. This probe is the first example of a "caged" Si-rhodamine, exhibits higher photon counts compared to established localization microscopy dyes, and is sufficiently red-shifted to allow multicolor imaging. The dye is a useful label for super-resolution imaging and constitutes a new scaffold for far-red fluorogenic molecules.},
  language  = {en}
}