@article{ScheerHansmannFalketal.1986, author = {Scheer, Ulrich and Hansmann, Paul and Falk, Heinz and Sitte, Peter}, title = {Ultrastructural localization of DNA in two Cryptomonas species by use of a monoclonal DNA-antibody}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-39746}, year = {1986}, abstract = {Immunogold cytochemistry - DNA localization - Cryptomonas nucleomorph The distribution and subcellular localization of DNA in the unicellular alga Cryptomonas has been investigated electron-microscopically by indirect immunocytochemistry, using a monoclonal DNA antibody and a gold-Iabeled secondary antibody. This technique proved to be very sensitive and entirely specific. DNA could be demonstrated in four different compartments (nucleus, nucleomorph, plastid, and mitochondrion). Within the plastid, DNA is concentrated in stroma regions that are localized preferentially around the center of the organelle. The mitochondrion contains several isolated DNA-containing regions (nucleoids). Within the nucleus, most of the DNA is localized in the 'condensed' chromatin. DNA was also detectable in small areas of the nucleolus, whereas the interchromatin space of the nucleus appeared almost devoid of DNA. Within the nucleomorph, DNA is distributed inhomogeneously in the matrix. DNA could furthermore be detected in restricted areas of the 'fibrillogranular body' of the nucleomorph, resembling the situation encountered in the nucleol us. The presence of DNA and its characteristic distribution in the nucleomorph provide additional, strong evidence in favour of the interpretation of that organelle as the residual nucleus of a eukaryotic endosymbiont in Cryptomonas.}, subject = {Cytologie}, language = {en} } @article{Dandekar1986, author = {Dandekar, Thomas}, title = {Offenlegungsschrift ({\"u}ber einen Biosensor)}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-31683}, year = {1986}, abstract = {No abstract available}, language = {de} } @article{SchultzMetznerDandekaretal.1986, author = {Schultz, R{\"u}diger and Metzner, Katharina and Dandekar, Thomas and Gramsch, Christian}, title = {Opiates induce long-term increases in prodynorphin derived peptide levels in the guinea-pig myenteric plexus}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-29809}, year = {1986}, abstract = {No abstract available}, language = {en} } @article{HadjiolovaRoseScheer1986, author = {Hadjiolova, Krassimira and Rose, Kathleen M. and Scheer, Ulrich}, title = {Immunolocalization of nucleolar proteins after D-galactosamine-induced inhibition of transcription in rat hepatocytes}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-33205}, year = {1986}, abstract = {No abstract available}, language = {en} } @article{ReimerScheerPetersetal.1986, author = {Reimer, Georg and Scheer, Ulrich and Peters, Jan-Michael and Tan, Eng M.}, title = {Immunolocalization and partial characterization of a nucleolar autoantigen (PM-Scl) associated with polymyositis / scleroderma overlap syndromes.}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-33191}, year = {1986}, abstract = {Precipitating anti-PM-Sel antibodies are present in sera from patients with polymyositis. scleroderma. and polymyositis/scleroderma overlap syndromes. By indirect immunofluorescence microscopy. anti-PM-Scl antibodies stained the nucleolus in cells of different tissues and species. suggesting that the antigen is highly conserved. By electron microscopy, anti-PM-Scl antibodies reacted primarily with the granular component of the nuc1eolus. Drugs that inhibit rRNA synthesis had a marked effect on the expression of PM-Scl antigen. In actinomycin D-treated cells, immunofluorescence staining by anti-PM-Scl was sign{\"u}icantly reduced with residual staining restricted to the granular regions of nuc1eoli. Treatment with 5,6-dichloro-beta-D- ribofuranosylbenzimidazole (DRB) also selectively reduced nuc1eolar staining. On a molecular level, anti-PM-Sel antibodies precipitated 11 polypeptides with molecular weights (Mr) ranging from 110,000 to 20,000. The Mr 80,000 and 20.000 polypeptides were phosphorylated. Evidence suggests that the PM-Scl antigen complex may be related to a prerlbosomal particle.}, language = {en} } @article{Linsenmair1986, author = {Linsenmair, Karl Eduard}, title = {Adaptations of the reed frog Hyperbolius viridiflavus to its arid environment: II. Some aspects of the water economy of H. viridiflavus nitidulus under wet and dry ...}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-78395}, year = {1986}, abstract = {Adaptations to aridity ofthe reedfrog Hyperolius viridiflavus nitidulus, living in different parts of the seasonally very dry and hot West African savanna, are investigated ...}, subject = {Zoologie}, language = {en} } @article{Scheer1986, author = {Scheer, Ulrich}, title = {Das Chromatin : seine Struktur und Funktion}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-80790}, year = {1986}, abstract = {no abstract available}, subject = {Chromatin}, language = {de} } @article{Scheer1986, author = {Scheer, Ulrich}, title = {Injection of antibodies into the nucleus of amphibian oocytes: an experimental means of interfering with gene expression in the living cell}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-41182}, year = {1986}, abstract = {No abstract available}, language = {en} } @article{ZimmermannStopper1986, author = {Zimmermann, U. and Stopper, Helga}, title = {Elektrofusion und Elektropermeabilisierung von Zellen}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-86865}, year = {1986}, abstract = {No abstract available.}, subject = {Elektrofusion}, language = {de} } @article{Mahsberg1986, author = {Mahsberg, Dieter}, title = {Contact chemoreception of prey in hunting scorpions}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-45784}, year = {1986}, abstract = {Scorpions commonly are assumed to hunt on living prey. But under laboratory conditions they also respond very sensitively to dead insects lying on the substrate. In many cases the motionless prey is seized and consumed. It was investigated how this behavior can be elicited. The buthid scorpions Androctonus australis (L.) and Buthus occitanus (Am.) not only find motionless prey again which was stung but managed to escape before dying: They also respond to extracts of the cuticle of prey insects. After touching prey marks' either with the tips of the chelae fingers or the tarsi of the walking legs or the pectine organs specific responses (searching, seizing, feeding) are released at a high rate. Behavioral experiments demonstrate for the first time the chemosensitivity of the pectine organs for which only mechanosensitivity had been proofed formerly. Mechanical as well as contact chemical stimulation of these organs cause scorpions to orient towards the stimulus source which is grasped, retained and consumed or rejected depending on its quality. The probably responsible chemosensitive receptors are already described in the literature. The possible adaptive value and the biological significance of contact chemoreception in prey catching and in other aspects of the life of scorpions is discussed.}, subject = {Skorpion}, language = {en} } @article{MauelerEigenbrodtSchartl1987, author = {Maueler, W. and Eigenbrodt, E. and Schartl, Manfred}, title = {Intermediary metabolism of normal and tumorous tissue of Xiphophorus (Teleostei: Poeciliidae)}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-61855}, year = {1987}, abstract = {No abstract available}, subject = {Physiologische Chemie}, language = {en} } @article{BarnekowSchartl1987, author = {Barnekow, A. and Schartl, Manfred}, title = {Comparative studies on the src proto-oncogene and its gene product pp60\(^{c-src}\) in normal and neoplastic tissues of lower vertebrates}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-61869}, year = {1987}, abstract = {No abstract available}, subject = {Physiologische Chemie}, language = {en} } @article{KleenePfannerPfalleretal.1987, author = {Kleene, R. and Pfanner, N. and Pfaller, R. and Link, T. A. and Sebald, Walter and Neupert, W. and Tropschug, M.}, title = {Mitochondrial porin of Neurospora crassa: cDNA cloning, in vitro expression and import into mitochondria}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62566}, year = {1987}, abstract = {No abstract available}, subject = {Biochemie}, language = {en} } @article{RoemischTropschugSebaldetal.1987, author = {R{\"o}misch, J. and Tropschug, M. and Sebald, Walter and Weiss, H.}, title = {The primary structure of cytochrome c\(_1\) from Neurospora crassa}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62578}, year = {1987}, abstract = {No abstract available}, subject = {Biochemie}, language = {en} } @article{BarnekowPaulSchartl1987, author = {Barnekow, A. and Paul, E. and Schartl, Manfred}, title = {Expression of the c-src protooncogene in human skin tumors}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-61870}, year = {1987}, abstract = {No abstract available}, subject = {Physiologische Chemie}, language = {en} } @misc{ReimerRaskaTanetal.1987, author = {Reimer, Georg and Raska, Ivan and Tan, Eng M. and Scheer, Ulrich}, title = {Human autoantibodies: probes for nucleolus structure and function}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-41410}, year = {1987}, abstract = {No abstract available}, language = {en} } @article{BenaventeRoseReimeretal.1987, author = {Benavente, Ricardo and Rose, Kathleen M. and Reimer, Georg and H{\"u}gle-D{\"o}rr, Barbara and Scheer, Ulrich}, title = {Inhibition of nucleolar reformation after microinjection of antibodies to RNA polymerase I into mitotic cells}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-33247}, year = {1987}, abstract = {The formation of daughter nuclei and the reformation of nucleolar structures was studied after microinjection of antibodies to RNA polymerase I into dividing cultured cells (PtK2). The fate of several nucleolar proteins representing the three main structural subcomponents of the nucleolus was examined by immunofluorescence and electron microscopy. The results show that the RNA polymerase I antibodies do not interfere with normal mitotic progression or the early steps of nucleologenesis, i.e. , the aggregation of nucleolar material into prenucleolar bodies. However,they inhibit the telophasic coalescence of the prenucleolar bodies into the chromosomal nucleolar organizer regions, thus preventing the formation of new nucleoli. These prenucleolar bodies show a fibrillar organization that also compositionally resembles the dense fibrillar component of interphase nucleoli . We conclude that during normal nucleologenesis the dense fibrillar component forms from preformed entities around nucleolar organizer regions, and that this association seems to be dependent on the presence of an active form of RNA polymerase I.}, language = {en} } @incollection{Scheer1987, author = {Scheer, Ulrich}, title = {Contributions of electron microscopic spreading preparations ("Miller-spreads") to the analysis of chromosome structure}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-39625}, publisher = {Universit{\"a}t W{\"u}rzburg}, year = {1987}, abstract = {No abstract available}, subject = {Eukaryonten / Chromosom}, language = {en} } @article{ScheerRaska1987, author = {Scheer, Ulrich and Raska, I.}, title = {Immunocytochemical localization of RNA polymerase I in the fibrillar centers of nucleoli}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-39618}, year = {1987}, abstract = {No abstract available}, language = {en} } @article{Scheer1987, author = {Scheer, Ulrich}, title = {Structure of lampbrush chromosome loops during different states of transcriptional activity as visualized in the presence of physiological salt concentrations}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-39304}, year = {1987}, abstract = {Lampbrush chromosomes of amphibian oocytes were isolated in the presence of near-physiological salt concentrations, to preserve their native state, and studied by electron microscopy of ultrathin s~dions. The transcriptional state of the lampbrush chromosomes was experimentally modulated by incubating the oocytes for various time periods in medium containing actinomycin D. The observations show that the structure of the lateral loops changes rapidly in response to alterations in transcriptional activity. During decreasing transcriptional activity and reduced packing density of transcripts, the chromatin axis first condensed into nucleosomes and then into an approximately 30 nm thick higher order chromatin fiber. Packaging of the loop axis into supranucleosomal structures may contribute to the foreshortening and retraction of the loops observed during inhibition of transcription and in later stages of meiotic prophase. The increasing packing density of the DNA during the retraction process of the loops could also be visualized by immunofluorescence microscopy using antibodies to DNA. The dependence of the loop chromatin structure on transcriptional activity is discussed in relation to current views of mechanisms involved in gene activation.}, language = {en} } @article{DandekarSchulz1987, author = {Dandekar, Thomas and Schulz, R.}, title = {Evidence for the expression of peptides derived from three opioid precursors in NG 108CC15 hybrid cells}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-29909}, year = {1987}, abstract = {No abstract available}, language = {en} } @article{MaschwitzFialaDolling1987, author = {Maschwitz, U. and Fiala, Brigitte and Dolling, W. R.}, title = {New trophobiotic symbioses of ants with South East Asian bugs}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-34030}, year = {1987}, abstract = {A trophobiotic relationship between two species of phloem-feeding plataspid bugs and an ant, Meranoplus mucronatus, was discovered on tree trunks in Malaysia. Similar relationships were found between coreid bugs and Crematogaster sp. and Anoplolepis longipes, on bamboo in the same area. The ants recruit to groups of the bugs and feed on the liquid, sugar-rich faeces of the larvae, stimulating release of the honeydew by tactile signals. They protect all stages of the bugs from disturbance by biting and by the use of defensive secretions. Phloem-feeding bugs in the families Plataspidae and Coreidae need long sty lets to pierce the thick bark of their host tree. The different methods of accommodating the resting stylets in these two families are described. The plataspids are described as Tropidotylus servus sp. novo and T. minister sp. novo A coreid previously reported in association with M. mucronatus in Malaya is described as Hygia cliens sp. novo The coreids on bamboo were determined as Cloresmus spp. and Notobitus affinis.}, language = {en} } @article{ReimerRoseScheeretal.1987, author = {Reimer, Georg and Rose, Kathleen M. and Scheer, Ulrich and Tan, Eng M.}, title = {Autoantibody to RNA polymerase I in scleroderma sera}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-34294}, year = {1987}, abstract = {Autoantibodies to components of the nucleolus are a unique serological feature of patients with scleroderma. There are autoantibodies of several specificities; one type produces a speckled pattern of nucleolar staining in immunofluorescence. In actinomycin D and 5,6-dichloro-{j-D-ribofuranosylbenzimidazoletreated Vero cells, staining was restricted to the fibrillar and not the granular regions. By double immunofluorescence, specific rabbit anti-RNA polymerase I antibodies stained the same fibrillar structures in drug-segregated nucleoli as scleroderma sera. Scleroderma sera immunoprecipitated 13 polypeptides from (35S)methionine-labeled HeLa cell extract with molecular weights ranging from 210,000 to 14,000. Similar polypeptides were precipitated by rabbit anti-RNA polymerase I antibodies, and their common identities were confirmed in immunoabsorption experiments. Microinjection of purified IgG from a patient with speckled nucleolar staining effectively inhibited ribosomal RNA transcription. Autoantibodies to RNA polymerase I were restricted to certain patients with scleroderma and were not found in other autoimmune diseases.}, language = {en} } @article{ScheerMessnerHazanetal.1987, author = {Scheer, Ulrich and Messner, Karin and Hazan, Rachel and Raska, Ivan and Hansmann, Paul and Falk, Heinz and Spiess, Eberhard and Franke, Werner W.}, title = {High sensitivity immunolocalization of double and single-stranded DNA by a monoclonal antibody}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-41063}, year = {1987}, abstract = {A monoclonal antibody (AK 30-10) is described which specifically reacts with DNA both in double and single-stranded forms but not with other molecules and structures, including deoxyribonucleotides and RNAs. When used in immunocytochemical experiments on tissue sections and permeabilized cultured cells, this antibody detects DNA-containing structures, even when the DNA is present in very small amounts. Examples of high resolution detection include the DNA present in amplified extrachromosomal nucleoli, chromomeres of lampbrush chromosomes, mitochondria, chloroplasts and mycoplasmal particles. In immunoelectron microscopy using the immunogold technique, the DNA was localized in distinct substructures such as the "fibrillar centers" of nucleoli and certain stromal centers in chloroplasts. The antibody also reacts with DNA of chromatin of living cells, as shown by microinjection into cultured mitotic cells and into nuclei of amphibian oocytes. The potential value and the limitations of immunocytochemical DNA detection are discussed.}, subject = {Cytologie}, language = {en} } @article{SchartlSchroeder1987, author = {Schartl, Manfred and Schr{\"o}der, Johannes Horst}, title = {A new species of the genus Xiphophorus Heckel 1848, endemic to northern Coahuila, Mexico (Pisces: Poeciliidae)}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-87117}, year = {1987}, abstract = {Xiphophorus meyeri n. sp. is described as an endemic to Muzquiz, Coahuila, Mexico. It appears to be the northernmost species of the genus. The new species is related to X. couchianus and X. gordoni, but differs morphologically from those by dorsal fin ray number, by the expression of some gonopodial features and most markedly by the appearance of macromelanophores or tr-melanophores.}, subject = {Schwertkr{\"a}pfling}, language = {en} } @incollection{ZimmermannStopper1987, author = {Zimmermann, U. and Stopper, Helga}, title = {Electrofusion and electropermeabilization of cells}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-73065}, publisher = {Universit{\"a}t W{\"u}rzburg}, year = {1987}, abstract = {No abstract available.}, subject = {Elektrofusion}, language = {en} } @article{PeschkeMahsberggebKrapfFuldner1987, author = {Peschke, Klaus and Mahsberg [geb. Krapf], Dieter and Fuldner, Dietrich}, title = {Ecological separation, functional relationships, and limiting resources in a carrion insect community}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-86361}, year = {1987}, abstract = {1. Thr ecological separation of 19 carrion inscet species (adults and some of their larvae) was investigated at rabbit carcasses in North Bavaria (FRG) referring to 4 niche dimensions. In the (1.) macrohabitats (forest - clearing) the distribution of saprophageous beetle larvae was mainly considered, for (2.) seasonality the differential abundance of blow flies (Colliphoridae). (3.) The stages of decay were correlated with the temperature dependent development of blow fly maggots affecting the abundance of competing saprophageous beetles and of carnivors preying upon maggots of different size classes. By using ( 4.) microhabitats (spatial subdivision of a carcass) as further niche dimension, the dustering of speries using similar food resources was domonstrated in a niche overlap dendrogram. 2. The quantitative effect of predators on blow fly maggots was investigated both in field and laboratory experiments. Predation upon maggots rcduces their scramble competition, resulting in a higher pupal weight. Thus, the reproductive succcss of the blow flies seems to be buffered by the developmental flexibility of the calliphorids. The numerical effect of predators and parasitoids on the blow fly pupae was also quantified. 3. In a case study on the staphylinid beetle, Aleochara curtula, we investigated the diffenntial abundance of sexes. The ratio at which the males and females arrive at the carcass is balanced. Here the beetles feed and copulated. Females depart into the vicinity of the carrion much earlier than males, thus shifting the sex ratio to a maale bias. In the surroundings the females deposit their eggs, and the parasitoid first instar larvae search for scattered blow fly pupae. The temporal and spatial distribution of both sexes of A. curtula is thus not only affected by the food allocation of the adults, but a.lso by limiting resources of mating end egg laying sites as well as larval hosts.}, subject = {Aask{\"a}fer}, language = {en} } @article{FoersterSchartl1987, author = {Foerster, Wolfgang and Schartl, Manfred}, title = {Karyotype and isozyme patterns of five species of Aulonocara REGAN, 1922}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-86774}, year = {1987}, abstract = {No abstract available.}, subject = {Aulonocara}, language = {en} } @article{AdamWittbrodtTellingetal.1988, author = {Adam, D. and Wittbrodt, J. and Telling, A. and Schartl, Manfred}, title = {RFLP for an EGF-receptor related gene associated with the melanoma oncogene locus of Xiphophorus maculatus}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-61822}, year = {1988}, abstract = {No abstract available}, subject = {Physiologische Chemie}, language = {en} } @article{SchartlPeter1988, author = {Schartl, Manfred and Peter, R. U.}, title = {Progressive growth of fish tumors after transplantation into thymus-aplastic (nu/nu) mice}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-61833}, year = {1988}, abstract = {No abstract available}, subject = {Physiologische Chemie}, language = {en} } @article{Schartl1988, author = {Schartl, Manfred}, title = {A sex chromosomal restriction-fragment-length marker linked to melanoma-determining Tu loci in Xiphophorus}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-61842}, year = {1988}, abstract = {No abstract available}, subject = {Physiologische Chemie}, language = {en} } @article{GesslerBruns1988, author = {Gessler, Manfred and Bruns, Gail A. P.}, title = {Molecular mapping and cloning of the breakpoints of a chromosome 11p14.1-p13 deletion associated with the AGR syndrome}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-59264}, year = {1988}, abstract = {Chromosome 11p13 is frequently rearranged in individuals with the WAGR syndrome (Wilms tumor, aniridia, genitourinary anomalies, and mental retardation) or parts of this syndrome. To map the cytogenetic aberrations molecularly, we screened DNA from cell Unes with known WAGR-related chromosome abnormalities for rearrangements with pulsed fleld gel (PFG) analysis using probes deleted from one chromosome 11 homolog of a WAGR patient. The first alteration was detected in a cell line from an individual with aniridia, genitourinary anomalies, mental retardation, and a deletion described as 11p14.1-p13. We have located one breakpoint close to probe HU11-164B and we have cloned both breakpoint sites as well as the junctional fragment. The breakpoints subdivide current intervals on the genetic map, and the probes for both sides will serve as important additional markers for a long-range restriction map of this region. Further characterization and sequencing of the breakpoints may yield insight into the mechanisms by which these deletions occur.}, subject = {Biochemie}, language = {en} } @article{GoebelChakrabortyKreft1988, author = {Goebel, Werner and Chakraborty, T. and Kreft, J{\"u}rgen}, title = {Bacterial hemolysins as virulence factors}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-60553}, year = {1988}, abstract = {No abstract available}, subject = {Biologie}, language = {en} } @article{GoebelKathariouKuhnetal.1988, author = {Goebel, Werner and Kathariou, S. and Kuhn, M. and Sokolovic, Z. and Kreft, J{\"u}rgen and K{\"o}hler, S. and Funke, D. and Chakraborty, T. and Leimeister-W{\"a}chter, M.}, title = {Hemolysin from Listeria-biochemistry, genetics and function in pathogenesis}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-60563}, year = {1988}, abstract = {No abstract available}, subject = {Biologie}, language = {en} } @article{ThiryScheerGoessens1988, author = {Thiry, Marc and Scheer, Ulrich and Goessens, Guy}, title = {Immunoelectron microscopic study of nucleolar DNA during mitosis in Ehrlich tumour cells}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-40745}, year = {1988}, abstract = {In order to investigate the DNA localization within Ehrlich tumor cell nucleoli during mitosis, two recent immunocytochemical methods using either an anti-DNA or an anti-bromodeoxyuridine (BrdU) monoclonal antibody have been applied. In both cases, the immunogold labeling has been performed on ultrathin sections of cells embedded either in Lowicryl K4M or in Epon, respectively. Identical results are observed with both immunocytochemical approaches. In the interphase nucleolus, besides the labeling of the perinucleolar chromatin shell and of its intranucleolar invaginations which penetrate into the nucleolar body and often terminate at the fibrillar centers, a few gold particles are also preferentially found towards the peripheral region of the fibrillar centers. In contrast, the dense fibrillar component and the granular component are never labeled. During mitosis, the fibrillar centers persist at the chromosomal nucleolus organizing regions (NOR's) and can be selectively stained by the silver method. However, these metaphase fibrillar centers are no longer decorated by the DNA- or BrdU antibodies. These results indicate that until the end of prophase, rRNA genes are present inside the fibrillar center material, disappear during metaphase and reappear in reconstituting nucleoli during telophase. Thus, fibrillar centers appear to represent structures sui generis, which are populated by rRNA genes only when the nucleolus is functionally active. In segregated nucleoli after actinomycin D treatment, the DNA labeling is exclusively restricted to the perinucleolar chromatin blocks. These findings also suggest that the DNA content of the fibrillar center material varies according to the rRNA transcription level of the cells. The results are discussed in the light of the present knowledge of the functional organization of the nucleolus.}, subject = {Cytologie}, language = {en} } @incollection{LinsenmairMikula1988, author = {Linsenmair, Karl Eduard and Mikula, Gerold}, title = {Soziale Einfl{\"u}sse}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-44469}, publisher = {Universit{\"a}t W{\"u}rzburg}, year = {1988}, abstract = {No abstract available}, language = {de} } @incollection{LinsenmairTyrellSchulzeetal.1988, author = {Linsenmair, Karl Eduard and Tyrell, Hartmann and Schulze, Hans-Joachim and M{\"u}ller, Klaus E.}, title = {Familie und Kleingruppen}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-44451}, publisher = {Universit{\"a}t W{\"u}rzburg}, year = {1988}, abstract = {No abstract available}, subject = {Psychobiologie}, language = {de} } @article{RoseSzopaHanetal.1988, author = {Rose, Kathleen M. and Szopa, Jan and Han, Fu-Sheng and Cheng, Yung-Chi and Richter, Arndt and Scheer, Ulrich}, title = {Association of DNA topoisomerase I and RNA polymerase I: A possible role for topoisomerase I in ribosomal gene transcription}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-33901}, year = {1988}, abstract = {RNA polymerase I preparations purified from a rat hepatoma contained DNA topoisomerase activity. The DNA topoisomerase associated with the polymerase had an Mr of 110000, required Mg2+ but not ATP, and was recognized by anti-topoisomerase I antibodies. When added to RNA polymerase I preparations containing topoisomerase activity, anti-topoisomerase I antibodies were able to inhibit the DNA relaxing activity of the preparation as well as RNA synthesis in vitro. RNA polymerase II prepared by analogous procedures did not contain topoisomerase activity and was not recognized by the antibodies. The topoisomerase I: polymerase I complex was reversibly dissociated by column chromatography on Sephacryl S200 in the presence of 0.25 M (NH4hS04. Topoisomerase I was immunolocalized in the transcriptionally active ribosomal gene complex containing RNA polymerase I in situ. These data indicate that topoisomerase I and RNA polymerase I are tightly complexed both in vivo and in vitro, and suggest a role for DNA topoisomerase I in the transcription of ribosomal genes.}, language = {en} } @article{ReimerRaskaScheeretal.1988, author = {Reimer, Georg and Raska, Ivan and Scheer, Ulrich and Tan, Eng M.}, title = {Immunolocalization of 7-2-ribonucleoprotein in the granular component of the nucleolus}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-33890}, year = {1988}, abstract = {Certain autoimmune sera contain antibodies against a nucleolar ribonucleoprotein particle associated with 7-2-RNA (R. Reddy et al. (1983) J. Bioi. Chem . 258, 1383; C. Hashimoto and J. A. Steitz (1983) J. Bioi. Chem. 258, 1379). In this study, we showed by immunofluorescence microscopy that antibodies reactive with 7-2-ribonucleoprotein immunolocalized in the granular regions of actinomycin D and 5,6-dichloro-I-j3-D-ribofuranosylbenzimidazole (DRB)-segregated nucleoli from Vero cells. By electron microscopic immunocytochemistry, antigen-antibody complexes were located in the granular component of transcriptionally active nucleoli from rat liver hepatocytes and HeLa cells. Anti-7- 2-RNP antibodies from two autoimmune sera immunoprecipitated a major protein of Mr 40,000 from e5S] methionine-Iabeled HeLa cell extract. The immunolocalization data suggest that 7-2-ribonucleoprotein may be involved in stages of ribosome biogenesis which take place in the granular component of the nucleolus, i.e., assembly, maturation, and/or transport of preribosomes}, language = {en} } @article{BenaventeSchmidtZachmannHuegleDoerretal.1988, author = {Benavente, Ricardo and Schmidt-Zachmann, Marion S. and H{\"u}gle-D{\"o}rr, B. and Reimer, G. and Rose, K. M. and Scheer, Ulrich}, title = {Identification and definition of nucleolus-related fibrillar bodies in micronucleated cells}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-39423}, year = {1988}, abstract = {Small nucleolus-related bodies which occur in the nUcleoplasm of " micronuclei" lacking nucleolar organizers have been studied by immunofluorescence microscopy. These bodies stained specifically with three different antibodies directed against proteins that are normally associated with the dense fibrillar component of functional nucleoli, but not with antibodies specific for certain proteins of the granular component or the fibrillar centers. Our data show that, in the absence of rRNA genes, the various constituent proteins characteristic of the dense fibrillar component spontaneously assemble into spherical entities but that the subsequent fusion of these bodies into larger structures is prevented in these micronuclei. The similarity between these nucleolus-related bodies of micronuclei and the prenucleolar bodies characteristic of early stages of nucleologenesis during mitotic telophase is discussed.}, language = {en} } @article{ThiryScheerGoessens1988, author = {Thiry, Marc and Scheer, Ulrich and Goessens, Guy}, title = {Localization of DNA within Ehrlich tumour cells nucleoli by immunoelectron microscopy}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-39327}, year = {1988}, abstract = {The distribution of DNA in Ehrlich tumour cell nucleoli was investigated by means of an immunocytochemical approach , involving a monoclonal antibody directed against double- and single-stranded DNA. Immunolabelling was performed . either before or after the embedding process. The postembedding labelling method allows better ultrastructural preservation than the preembedding labelling method. In particular, the various nucleolar components are well preserved and identifiable. In the nucleolus, labelling is particularly concentrated over the perinucleolar chromatin and over its intranucleolar invaginations, which penetrate the nucleolar body and often terminate at the fibrillar centres. In addition, aggregates of gold particles are found in the fibrillar centres, preferentially towards the peripheral regions. By contrast, the dense fibrillar component is completely devoid of labelling. The results seem to indicate that DNA containing the rDNA genes is located in the fibrillar centres, with a preference for the peripheral regions. This finding suggests that transcription of the rDNA genes should occur within the confines of the fibrillar centre, probably close to the boundary region of the surrounding dense fibrillar component. The results are discussed in the light of present knowledge of the functional organization of the nucleolus.}, language = {en} } @article{GeiseLinsenmair1988, author = {Geise, W. and Linsenmair, Karl Eduard}, title = {Adaptations of the reed frog Hyperbolius viridiflavus to its arid environment. IV. Ecological significance of water economy with comments on thermoregulation and energy allocation}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-30570}, year = {1988}, abstract = {No abstract available}, language = {en} } @article{DabauvalleSchulzScheeretal.1988, author = {Dabauvalle, Marie-Christine and Schulz, Barbara and Scheer, Ulrich and Peters, Reiner}, title = {Inhibition of nuclear accumulation of karyophilic proteins in living cells by microinjection of the lectin wheat germ agglutinin}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-34288}, year = {1988}, abstract = {No abstract available}, language = {en} } @article{ScheerDabauvalleMerkertetal.1988, author = {Scheer, Ulrich and Dabauvalle, Marie-Christine and Merkert, Hilde and Benavente, Ricardo}, title = {The nuclear envelope and the organization of the pore complexes}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-34275}, year = {1988}, abstract = {No abstract available}, language = {en} } @article{LinsenmairSchmuck1988, author = {Linsenmair, Karl Eduard and Schmuck, R.}, title = {Adaptations of the reed frog Hyperbolius viridiflavus to its arid environment. III. Aspects of nitrogen metabolism and osmuregulation in the reed frog, H. viridiflavus taeniatus, with special reference to the role of iridophores}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-78108}, year = {1988}, abstract = {Reed frogs of the superspecies Hyperolius viridiflavus occur throughout the seasonally very dry and hot African savannas. Despite their small size (300-700 mg), estivating reed frogs do not avoid stressful conditions above ground by burrowing into the soil, but endure the inhospitable climate relatively unprotected, clinging to mostly dry grass sterns. They must have emcient mechanisms to enable them to survive e.g. very high temperatures, low relative hurnidities, and high solar radiation loads. Mechanisms must also have developed to prevent poisoning by the nitrogenous wastes that inevitably result from protein and nucleotide turnover. In contrast to fossorial amphibians, estivating reed frogs do not become torpid. Reduction in metabolism is therefore rather Iimited so that nitrogenous wastes accumulate faster in these frogs than in fossorial amphibians. This severely aggravates the osmotic problems caused by dehydration. During dry periods total plasma osmolarity greatly increases, mainly due to urea accumulation. Of the total urea accumulated over 42 days of experimental water deprivation, 30\% was produced during the first 7 days. In the next 7 days rise in plasma urea content was negligible. This strong initial increase of urea is seen as a byproduct of elevated amino acid catabolism following the onset of dry conditions. Tbe rise in total plasma osmolarity due to urea accumulation, however, is not totally disadvantageous, but enables fast rehydration when water is available for very short periods only. Voiding of urine and feces eeases once evaporative water loss exceeds 10\% of body weight. Tberefore, during continuous water deprivation, nitrogenous end products are not excreted. After 42 days of water deprivation, bladder fluid was substantially depleted, and urea coneentration in the remaining urine (up to 447 mM) was never greater than in plasma fluid. Feces voided at the end of the dry period after water uptake contained only small amounts of nitrogenous end products. DSF (dry season frogs) seemed not to be uricotelic. Instead, up to 35\% of the total nitrogenous wastes produced over 42 days of water deprivation were deposited in an osmotically inert and nontoxic form in iridophore crystals. The increase in skin purine content averaged 150 µg/mg dry weight. If urea had been the only nitrogenous waste product during an estivation period of 42 days, lethal limits of total osmolarity (about 700 mOsm) would have been reached 10-14 days earlier. Thus iridophores are not only involved in colour change and in reducing heat load by radiation remission, but are also important in osmoregulation during dry periods. The seIective advantages of deposition of guanine rather than uric acid are discussed.}, subject = {Biologie}, language = {en} } @article{SchmuckKobeltLinsenmair1988, author = {Schmuck, R. and Kobelt, F. and Linsenmair, Karl Eduard}, title = {Adaptations of the reed frog Hyperbolius viridiflavus (Anura, Hyperbolidae) to its arid environment: V. Iridophores and nitrogen metabolism}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-78094}, year = {1988}, abstract = {Ofall amphibians living in arid habitats, reed frogs (belonging to the super species Hyperolius viridiflavus) are the most peculiar. Froglets are able to tolerate dry periods of up to 35 days or longer immediately after metamorphosis, in climatically exposed positions. They face similar problems to estivating juveniles, i.e. enduranee of long periods of high temperature and low RH with rather limited energy and water reserves. In addition, they must have had to develop meehanisms to prevent poisoning by nitrogenous wastes that rapidly accumulate during dry periods as a metabolie consequenee of maintaining a non-torpid state. During dry periods, plasma osmolarity of H. v. taeniatus froglets strongly increased, mainly through urea accumulation. Urea accumulation was also observed during metamorphic climax. During postmetamorphic growth, chromatophores develop with the density and morphology typical of the adult pigmentary pattern. The dermal iridophore layer, which is still incomplete at this time, is fully developed within 4-8 days after metamorphosis, irrespective of maintenance conditions. These iridophores mainly contain the purines guanine and hypoxanthine. The ability of these purines to reflect light provides an excellent basis for the role of iridophores in temperature regulation. In individuals experiencing dehydration stress, the initial rate of purine synthesis is doubled in eomparison to specimens continuously maintained under wet season conditions. This increase in synthesis rate leads to a rapid increase in the thiekness of the iridophore layer, thereby effectively reducing radiation absorption. Thus, the danger of overheating is diminished during periods of water shortage when evaporative cooling must be avoided. After the development of an iridophore layer of sufficient thickness for effective radiation reflectance, synthesis of iridophore pigments does not cease. Rather, this pathway is further used during the remaining dry season for solving osmotic problems eaused by accumulation of nitrogenous wastes. During prolonged water deprivation, in spite of reduced metabolic rates, purine pigments are produced at the same rate as in wet season conditions. This leads to a higher relative proportion of nitrogen end products being stored in skin pigments under dry season conditions. At the end of an experimental dry season lasting 35 days, up to 38\% of the accrued nitrogen is stored in the form of osmotically inactive purines in thc skin. Thus the osmotic problems caused by evaporative water loss and urea production are greatly reduced.}, subject = {Biologie}, language = {en} } @article{MaeuelerRaulfSchartl1988, author = {M{\"a}ueler, Winfried and Raulf, Friedrich and Schartl, Manfred}, title = {Expression of proto-oncogenes in embryonic, adult, and transformed tissue of Xiphophorus (Teleostei: Poeciliidae)}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-86233}, year = {1988}, abstract = {In Xiphophorus the causative, primary cellular oncogene for melanoma formation has been assigned by classical genetics to a sex-chromosomal locus, designated Tu. Activation of Tu was proposed to be the result of the elimination of Tu-specific regulatory genes which normally suppress the transforming function in the nontumorous state. In order to understand the role which known proto-oncogenes migbt play in this process, we have analysed the expression of src, erb A, erb B, ras, abl, sis and mil related genes from Xiphophorus during embryogenesis, in non-tumorous organs and in melanoma cells. For src, ras, erb B and sis a differential expression during embryogenesis and/or in normal organs was detected, with preferential expression of src in neural tissues, a high abundance of sis transcripts in an embryonal epitheloid cellline and of erbB transcripts in the head nephros. In melanoma cells ras, src and a v-erb B related gene were found to be expressed. The src gene most likely is more involved in secondary processes during tumor progression, while the expression of the v-erb B related gene might be transformation-specific because recently such a sequence was found to map to the close vicinity of the Tu-locus.}, subject = {Schwertk{\"a}rpfling}, language = {en} } @article{MauelerBarnekowEigenbrodtetal.1988, author = {Maueler, W. and Barnekow, A. and Eigenbrodt, E. and Raulf, F. and Falk, H. F. and Telling, A. and Schartl, Manfred}, title = {Different regulation of oncogene expression in tumor and embryonal cells of Xiphophorus}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-86240}, year = {1988}, abstract = {Melanoma formation in the poeciliid fish Xiphophorus is mediated primarily by a cellular oncogene, designated Tu. Elimination of Tu-specific genes releases the transforming function of Tu and leads to melanoma formation. Southern blot analyses revealed a tight linkage of a v-erb B related gene to the Tu-locus and Northern blot analyses of RNA of solid melanomas indicated a coordinated deregulation and for mutational activation of several oncogenes. In order to get a better insight into the regulation of oncogene expression in normal and transformed cells of Xiphophorus, we studied the expression of Xsrc, Xras, Xmyc, Xerb A, Xsis, and the v-erb B related gene in a melanoma derived cell line (PSM) and an embryonic cell line (A2) under conditions of low growth factor supply. Both celllines express the Xsrc, Xmyc, and Xras genes, while PSM cells in addition express the v-erb B related gene and A2 cells the Xsis gene. In PSM cells serum deprivation leads to an accumulation of most of the oncogene mRNAs analysed. This is most apparent for a 5.0 kb transcript of the v-erb B related gene, probably due to an increase in transcript stability. The levels of these mRNAs returned to normal within 2h after stimulation with 10\% fetal calf serum. At the protein level we observed an initial decrease followed by an increase of the n-p60c-src kinase (the protein product of tbe Xsrc gene) activity in cells deprived of serum. Serum stimulation restored a normal pp60"-src kinase activity. In contrast serum deprivation of A2 cells reduced the transcript amounts of each of the oncogenes analysed. The same holds true for one beta-tubulin transcript, while the level of a second beta-tubulin transcript was unaffected. Serum stimulation led to a reactivation of Xras and Xsrc after a delay of approximately 48b. The pp60(c-src) kinase activity was found to be 6-10 times lower as compared to the PSM cells and did not differ between serum deprived and serum stimulated cells. Enzyme activities and isoenzyme patterns of several glycolytic enzymes were found to be not affected by serum deprivation and stimulation in both celllines.}, subject = {Schwertk{\"a}rpfling}, language = {en} } @article{BenaventeReimerRoseetal.1988, author = {Benavente, Ricardo and Reimer, Georg and Rose, Kathleen M. and H{\"u}gle-D{\"o}rr, Barbara and Scheer, Ulrich}, title = {Nucleolar changes after microinjection of antibodies to RNA polymerase I into the nucleus of mammalian cells}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-40666}, year = {1988}, abstract = {After microinjection of antibodies against RNA polymerase I into the nuclei of cultured rat kangaroo (PtKz) and rat (RVF-SMC) cells alterations in nucleolar structure and composition were observed. These were detected by electron microscopy and double-label immunofluorescence microscopy using antibodies to proteins representative of the three major components of the nucleolus. The microinjected antibodies produced a progressive loss of the material of the dense fibrillar component (DFC) from the nucleoli which, at 4 h after injection, were transformed into bodies with purely granular component (GC) structure with attached fibrillar centers (FCs). Concomitantly, numerous extranucleolar aggregates appeared in the nucleoplasm which morphologically resembled fragments of the DFC and contained a protein (fibrillarin) diagnostic for this nucleolar structure. These observations indicate that the topological distribution of the material constituting the DFC can be experimentally influenced in interphase cells, apparently by modulating the transcriptional activity of the rRNA genes. These effects are different from nucleolar lesions induced by inhibitory drugs such as actinomycin D-dependent "nucleolar segregation". The structural alterations induced by antibodies to RNA polymerase I resemble, however, the initial events of nucleolar disintegration during mitotic prophase.}, language = {en} } @inproceedings{SchartlMaeuelerRaulfetal.1988, author = {Schartl, Manfred and M{\"a}ueler, Winfried and Raulf, Friedrich and Robertson, Scott M.}, title = {Molecular aspects of melanoma formation in Xiphophorus}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-72689}, year = {1988}, abstract = {No abstract available.}, subject = {Schwertk{\"a}rpfling}, language = {en} } @article{RaulfRobertsonSchartl1989, author = {Raulf, F. and Robertson, S. M. and Schartl, Manfred}, title = {Evolution of the neuron-specific alternative splicing product of the c-src proto-oncogene}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-61796}, year = {1989}, abstract = {The observation of a slower migrating form of pp6oc-src in neural tissue of chicken and mouse has recently been shown to be due to an alternative transcript form of tbe c-src gene (Martinez et al.: Science 237:411-415, 1987; Levy et al.: Mol Cell Bio17:4142- 4145, 1987). An insertion of 18 basepairs between exons 3 and 4, presumed to be due to alternative splicing of a mini-exon, gives rise to six amino acid residues not found in the non-neuronal (termed flbroblastic) form of pp60\(^{c-src}\). Wehave addressed the question of the evolutionary origin of the c-src neuronal insert · and its functional signiflcance regarding neural-speciflc expression of the c-src gene. To this end we have investigated whether the c-src gene of a lower verlebrate (the teleost fish Xiphophorus) gives rise to a neural-specific transcript in an analogous manner. We could show that the fish c-src gene does encode for a "fibroblastic" and a "neuronal" form of transcript and that the neuronal transcript does indeed arise by way of alternative splicing of a mini-exon. The miniexon is also 18 basepairs long and we could demoostrate directly that this exon lies within the intron separating exons 3 and 4. For comparative purposes we have examined whether the fish c-yes gene, the member of the src gene family most closely related to c-src, also encodes a neural tissue-specific transcript. No evidence for a second transcript form in brain was obtained. This result suggests that the mini-exon arose within the c-src gene lineage sometime between the srclyes gene duplication event and the divergence of the evolutionary lineage giving rise to the teleost fish. Published genomic sequence of src-related genes in Drosophila and our own results with Hydra demoostrate no intron in these species at the analogous location, consistent with first appearance of this mini-exon sometime between 550 and 400 million years ago.}, subject = {Physiologische Chemie}, language = {en} } @article{WittbrodtAdamMalitscheketal.1989, author = {Wittbrodt, J. and Adam, D. and Malitschek, B. and Maueler, W. and Raulf, F. and Telling, A. and Robertson, M. and Schartl, Manfred}, title = {Novel putative receptor tyrosine kinase encoded by the melanoma-inducing Tu locus in Xiphophorus}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-61800}, year = {1989}, abstract = {No abstract available}, subject = {Physiologische Chemie}, language = {en} } @article{BernardsSchacklefordGerberetal.1989, author = {Bernards, R. and Schackleford, G. M. and Gerber, M. R. and Horowitz, J. M. and Friend, S. H. and Schartl, Manfred and Bogenmann, E. and Rapaport, J. M. and Mcgee, T. and Dryja, T. P.}, title = {Structure and expression of the murine retinoblastoma gene and characterization of its encoded protein}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-61819}, year = {1989}, abstract = {No abstract available}, subject = {Physiologische Chemie}, language = {en} } @article{WeigelMeyerSebald1989, author = {Weigel, U. and Meyer, M. and Sebald, Walter}, title = {Mutant proteins of human interleukin 2. Renaturation yield, proliferative activity and receptor binding}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62543}, year = {1989}, abstract = {No abstract available}, subject = {Biochemie}, language = {en} } @article{FlueggeFischerGrossetal.1989, author = {Fl{\"u}gge, U. I. and Fischer, K. and Gross, A. and Sebald, Walter and Lottspeich, F. and Eckerskorn, C.}, title = {The triose phosphate-3-phosphoglycerate-phosphate translocator from spinach chloroplasts: nucleotide sequence of a full-length cDNA clone and import of the in vitro synthesized precursor protein into chloroplasts}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62559}, year = {1989}, abstract = {No abstract available}, subject = {Biochemie}, language = {en} } @article{KreftFunkeSchlesingeretal.1989, author = {Kreft, J{\"u}rgen and Funke, D. and Schlesinger, R. and Lottspeich, F. and Goebel, Werner}, title = {Purification and characterization of cytolysins from Listeria monocytogenes serovar 4b and Listeria ivanovii}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-47036}, year = {1989}, abstract = {Several exoproteins from Listeria monocytogenes serovar 4b (NCTC 10527) and Listeria ivanovii (ATCC) 19119, SLCC 2379), respectively, have been purified to homogeneity by thiol-disulfide exchange chromatography and gel filtration. Both strains produce a haemolytic/cytolytic protein of Mr 58 kDa, which has all the properties of a SH-activated cytolysin, the prototype of which is streptolysin 0 (SLO), and this protein has therefore heen termed Iisteriolysin 0 (LLO). In addition a protein of Mr 24 kDa from culture supernatants of L. ivanovii co-purified withLLO. The N-terminal aminoacid sequences of both proteins from L. ivanovii have been determined. By mutagenesis with transposons of Gram-positive origin (Tn916 and TnI545), which have been introduced via conjugation into L. ivanovii, several phenotypic mutants (altered haemolysis on sheep blood agar or lecithinase-negative) were obtained. Results on the properties of these muntants will he presented.}, language = {en} } @article{KreftHaasGoebel1989, author = {Kreft, J{\"u}rgen and Haas, Albert and Goebel, Werner}, title = {Isolation and characterization of genes coding for proteins involved in the cytolysis by Listeria ivanovii}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-46991}, year = {1989}, abstract = {We established a library of chromosomal DNA of Listeria ivanovii in the pTZ19R plasmid system, using Escherichia coli DH5alpha as the host. One recombinant clone reacted strongly with a polyclonal antiserum raised against the listeriolysin 0 and a second exoprotein (24kDa) of L. ivanovii, which is most probably also involved in cytolytic processes. The recombinant E. coli clone may contain part of the listeriolysin 0 gene of L. ivanovii.}, language = {en} } @article{GesslerBruns1989, author = {Gessler, Manfred and Bruns, G. A. P.}, title = {A physical map around the WAGR complex on the short arm of chromosome 11}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-59246}, year = {1989}, abstract = {A long-range restriction map of part of the short arm of ehromosome 11 including the WAGR region has been constructed using pulsed-field gel electrophoresis and a number of infrequently cutting restriction enzymes. A total of 15.4 Mbp has been mapped in detall, extending from proximal 11p14 to the distal part of 11p12. The map localizes 35 different DNA probes and reveals at least nine areas with features eharaeteristle of BTF islands, some of which may be candidates for the different loci underlying the phenotype of the WAGR syndrome. This map will furthermore allow screening of DNA from individuals with WAGR-related phenotypes and from Wilms tumors for associated chromosomal rearrangements.}, subject = {Biochemie}, language = {en} } @article{GesslerThomasCouillinetal.1989, author = {Gessler, Manfred and Thomas, G. H. and Couillin, P. and Junien, C. and McGillivray, B. C. and Hayden, M. and Jaschek, G. and Bruns, G. A.}, title = {A deletion map of the WAGR region on chromosome II}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-59255}, year = {1989}, abstract = {The WAGR (Wilms tumor, aniridia, genitourinary anomalies, and mental retardation) region has been assigned to chromosome 11p13 on the basis of overlapping constitutional deletions found in affected individuals. We have utilized 31 DNA probes which map to the WAGR deletion region, together with six reference loci and 13 WAGR-related deletions, to subdivide this area into 16 intervals. Specific intervals have been correlated with phenotypic features, leading to the identification of individual subregions for the aniridia and Wilms tumor loci. Delineation, by specific probes, of multiple intervals above and below the critical region and of five intervals within the overlap area provides a framework map for molecular characterization of WAGR gene loci and of deletion boundary regions.}, subject = {Biochemie}, language = {en} } @article{KreftFunkeHaasetal.1989, author = {Kreft, J{\"u}rgen and Funke, Dorothee and Haas, Albert and Lottspeich, Friedrich and Goebel, Werner}, title = {Production, purification and characterization of hemolysins from Listeria ivanovii and Listeria monocytogenes Sv4b.}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-60545}, year = {1989}, abstract = {In culture supematants of both Listeria ivanovii and Listeria monocytogenes Sv4b, for the first time a hemolysin of molecular weight 58 kDa was identified, which had all the characteristics of an SH-activated cytolysin, and which was therefore identified as Iisteriolysin 0 (LLO). In the case of L. ivanovii a second major supematant protein of molecular weight 24 kDa co-purified with LLO. However, the function of this protein has to be determined. In culture supematants of L. ivanovii a sphingomyelinase and a Iecithinase activity could be detected, both enzymatic activities together contributing to the pronounced hemolysis caused by L. ivanovii. The N-tenninal amino acid sequences of LLO and the 24 kDa from L. ivanovii are shown.}, subject = {Biologie}, language = {en} } @article{GilmoreCruzRodzLeimeisterWaechteretal.1989, author = {Gilmore, Michael S. and Cruz-Rodz, Armando L. and Leimeister-W{\"a}chter, Michaela and Kreft, J{\"u}rgen and Goebel, Werner}, title = {A Bacillus cereus cytolytic determinant, cereolysin AB, which comprises the phospholipase C and sphingomyelinase genes: nucleotide sequence and genetic linkage}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-60588}, year = {1989}, abstract = {A cloned cytolytic determinant from the genome of Bacillus cereus GP-4 has been characterized at the molecular Ievel. Nucleotide sequence determination revealed the presence of two open reading frames. 8oth open reading frames were found by deletion and complementation analysis to be necessary for expression of the hemolytic phenotype by Bacillus subtilis and Escherichia coli hosts. The 5' open reading frame was found to be nearly identical to a recently reported phospholipase C gene derived from a mutant B. cereus strain which overexpresses the respective protein, and it conferred a lecithinase-positive phenotype to the B. subtilis host. The 3' open reading frame encoded a sphingomyelinase. The two tandemly encoded activities, phospholipase C and sphingomyelinase, constitute a biologically functional cytolytic determinant of B. cereus termed cereolysin AB.}, subject = {Biologie}, language = {en} } @article{BenaventeDabauvalleScheeretal.1989, author = {Benavente, Ricardo and Dabauvalle, Marie-Christine and Scheer, Ulrich and Chaly, Nathalie}, title = {Functional role of newly formed pore complexes in postmitotic nuclear reorganization}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-40754}, year = {1989}, abstract = {Many nuclear proteins are released into the cytoplasm at prometaphase and are transported back into the daughter nuclei at the end of mitosis. To determine the role of this reentry in nuclear remodelling during early interphase, we experimentally manipulated nuclear protein uptake in dividing cells. Recently we and others have shown that signal-dependent, pore complex-mediated uptake of nuclear protein is blocked in living cells on microinjection of the lectin wheat germ agglutinin (WGA), or of antibodies such as PI1 that are directed against WGA-binding pore complex glycoproteins. In the present study, we microinjected mitotic PtKz cells with WGA or antibody PIt and followed nuclear reorganization of the daughter cells by immunofluorescence and electron microscopy. The inhibitory effect on nuclear protein uptake was monitored by co-injection of the karyophilic protein nucleoplasmin. When injected by itself early in mitosis, nucleoplasmin became sequestered into the daughter nuclei as they entered telophase. In contrast, nucleoplasmin was excluded from the daughter nuclei in the presence of WGA or antibody PI1 . Although PtKz cells with blocked nuclear protein uptake completed cytokinesis, their nuclei showed a telophaselike organization characterized by highly condensed chromatin surrounded by a nuclear envelope containing a few pore complexes. These findings suggest that pore complexes become functional as early as telophase, in close coincidence with nuclear envelope reformation. They further indicate that the extensive structural rearrangement of the nucleus during the telophase-G1 transition is dependent on the influx of karyophilic proteins from the cytoplasm through the pore complexes, and is not due solely to chromosome- associated components.}, language = {en} } @article{WeberSchmidtScheer1989, author = {Weber, Thomas and Schmidt, Erwin and Scheer, Ulrich}, title = {Mapping of transcription units on Xenopus laevis lampbrush chromosomes by in situ hybridization with biotin-labeled cDNA probes}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-40763}, year = {1989}, abstract = {A non-radioactive in situ hybridization method is described for the localization of transcription units of defined genes to lateral loops of Xenopus laevis lampbrush chromosomes. Two Xenopus cONA probes were used encoding the nucleolar protein N038/ B23 and cytokeratin 1(8). Both proteins are known to be synthesized in Xenopus oocytes, and Northern blot analysis revealed the presence of the corresponding mRNAs in different oogenic stages. The probes were enzymatically labeled with biotin-dCTP and hybridized to lampbrush chromosomes. The sites of hybridization were detected either by indirect immunofluorescence microscopy using rabbit antibodies against biotin and fluorescein-conjugated antirabbit IgG or enzymatically using peroxidase-conjugated streptavi din. The probe encoding the nucleolar protein hybridized to two sets of lateral loops on different bivalents, the cytokeratin probe to at least four. Our finding that each probe hybridized to more than one chromosomal locus may reflect the tetraploid nature of the Xenopus laevis genome or results from cross-hybridization to other transcriptionally active members of the N038/ B23-nucleoplasmin or the cytokeratin-Iamin gene families. The method described should facilitate further in situ hybridization studies with appropriate genomic clones in order to map specific DNA sequences to defined loop regions and to come to a better understanding of the relationship between loop organization and gene transcription unit.}, subject = {Cytologie}, language = {en} } @article{BenaventeScheerChaly1989, author = {Benavente, Ricardo and Scheer, Ulrich and Chaly, Nathalie}, title = {Nucleocytoplasmic sorting of macromolecules following mitosis: fate of nuclear constituents after inhibition of pore complex function}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-40777}, year = {1989}, abstract = {PtK2 cells in which pore complex-mediated transport is blocked by microinjection early in mitosis of a monoclonal antibody (specific for an Mr 68000 pore complex glycoprotein) or of wheat germ agglutinin (WGA) complete cytokinesis. However, their nuclei remain stably arrested in a telophase-like organization characterized by highly condensed chromatin and the absence of nucleoli, indicating a requirement for pore-mediated transport for the reassembly of interphase nuclei. We have now examined this requirement more closely by monitoring the behavior of individual nuclear macromolecules in microinjected cells using immunofluorescence microscopy and have investigated the effect of microinjecting the antibody or WGA on cellular ultrastructure. The absence of nuclear transport did not affect the sequestration into daughter nuclei of components such as DNA, DNA topoisomerase I and the nucleolar protein fibrillarin that are carried through mitosis on chromosomes. On the other hand, lamins, snRNAs and the p68 pore complex glycoprotein, all cytoplasmic during mitosis, remained largely cytoplasmic in the telophase-arrested cells. Electron microscopy showed the nuclei to be surrounded by a doublelayered membrane with some inserted pore complexes. In addition, however, a variety of membranous structures with associated pore complexes was regularly noted in the cytoplasm, suggesting that chromatin may not be essential for the postmitotic formation of pore complexes. We propose that cellular compartmentalization at telophase is a two-step process. First, a nuclear envelope tightly encloses the condensed chromosomes, excluding non-selectively all macromolecules not associated with the chromosomes. Interphase nuclear organization is then progressively restored by selective pore complex-mediated uptake of nuclear proteins from the cytoplasm.}, subject = {Cytologie}, language = {en} } @article{MaschwitzFialaLeeetal.1989, author = {Maschwitz, Ulich and Fiala, Brigitte and Lee, Ying Fah and Chey, Vun Khen and Tan, Fui Lian}, title = {New and little-known myrmecophytic associations from Bornean rain forests}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-42957}, year = {1989}, abstract = {The woody climber Millettia niuewenhuisii (Fabaceae) and the shrub Myrmeconauclea strigosa (Rubiaceae) in Sabah, Borneo are associated with ants. The hollow stems of Millettia nieuwenhuisii are regularly inhabited by an aggressive Cladomyrma sp., which keeps pseudococcids inside the stem. On Myrmeconauclea strigosa the ants live in hollow internodal swellings near the end of the branches. In this plant many different ant species use the nesting space in an opportunistic manner.}, language = {en} } @article{FialaMaschwitzPongetal.1989, author = {Fiala, Brigitte and Maschwitz, Ulrich and Pong, Tho, Yow and Helbig, Andreas J.}, title = {Studies of a South East Asian ant-plant association : protection of Macaranga trees by Crematogaster borneensis}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-42857}, year = {1989}, abstract = {In the humid tropics of SE Asia there are some 14 myrmecophytic species of the pioneer tree genus Macaranga (Euphorbiaceae). In Peninsular Malaysia a close association exists between the trees and the small, non-stinging myrmicine Crema togas ter borneensis. These ants feed mainly on food bodies provided by the plants and have their colonies inside the hollow intemodes. In a ten months field study we were able to demonstrate for four Macaranga species (M. triloba, M. hypoleuca, M. hosei, M. hulletti) that host plants also benefit considerably from ant-occupation. Ants do not contribute to the nutrient demands of their host plant, they do, however, protect it against herbivores and plant competition. Cleaning behaviour of the ants results in the removal of potential herbivores already in their earliest developmental stages. Strong aggressiveness and a mass recruiting system enable the ants to defend the host plant against many herbivorous insects. This results in a significant decrease in leaf damage due to herbivores on ant-occupied compared to ant-free myrmecophytes as well as compared to non-myrmecophytic Macaranga species. Most important is the ants' defense of the host plant against plant competitors, especially vines, which are abundant in the well-lit pioneer habitats where Macaranga grows. Ants bite off any foreign plant part coming into contact with their host plant. Both ant-free myrmecophytes and non-myrmecophytic Macaranga species had a significantly higher incidence of vine growth than specimens with active ant colonies. This may be a factor of considerable importance allowing Macaranga plants to grow at sites of strongest competition.}, language = {en} } @article{DandekarRibesTollervey1989, author = {Dandekar, Thomas and Ribes, V. and Tollervey, David}, title = {Schizosaccharomyces pombe U4 small nuclear RNA closely resembles vertebrate U4 and is required for growth}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-29771}, year = {1989}, abstract = {No abstract available}, language = {en} } @article{DandekarTollervey1989, author = {Dandekar, Thomas and Tollervey, David}, title = {Cloning of Schizosaccharomyces pombe genes encoding the U1,U2,U3 and U4 snRNAs}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-29919}, year = {1989}, abstract = {No abstract available}, language = {en} } @techreport{GesslerSimolaBruns1989, author = {Gessler, Manfred and Simola, Kalle O. and Bruns, Gail A. P.}, title = {Cloning of breakpoints of a chromosome translocation identifies the AN2 locus}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-30177}, year = {1989}, abstract = {Chromosome translocations involving llpl3 have been associated with familial aniridia in two kindreds highlighting the chromosomal localization of the AN2 locus. This locus is also part of the WAGR complex (Wilros tumor, aniridia, genitourinary abnormalities, and mental retardation). In one kindred, the translocation is associated with a deletion, and probes for this region were used to identify and clone the breakpoints of the translocation in the second kindred. Comparison of phage restriction maps exclude the presence of any sizable deletion in this case. Sequences at the chromosome 11 breakpoint are conserved in multiple species, suggesting that the translocation falls within the AN2 gene.}, language = {en} } @article{GrafeLinsenmair1989, author = {Grafe, U. and Linsenmair, Karl Eduard}, title = {Protogynous sex change in the Reed Frog: Hyperolius viridiflavus}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-30990}, year = {1989}, abstract = {Observations on captive reed frogs Hyperolius viridijlavus ommatostictus showed that seven out of 24 females changed into males. Sex change occurred without any hormone treatment and resulted in completely functional males. The adaptive value is discussed in terms of maximizing life-time reproductive success. Hyperolius r. ommatostictus is the first amphibian known to show functional sex reversal.}, language = {en} } @article{RaulfMaeuelerRobertsonetal.1989, author = {Raulf, Friedrich and M{\"a}ueler, Winfried and Robertson, Scott M. and Schartl, Manfred}, title = {Localization of cellular src mRNA during development and in the differentiated bipolar neurons of the adult neural retina in Xiphophorus}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-86703}, year = {1989}, abstract = {The expression of the c-src gene in embryonie and adult tissue of the teleost fish Xiphophorus helleri was analyzed by in-situ hybridization. The highly conserved fish c-src gene was found to be expressed at high levels in midterm embryos, where c-src mRNA was localized in developing neurons of the sensory layer of the differentiating retina and in the developing brain. In adult tissues the expression of c-src was found to persist in certain cell types of the brain and the neural retina, especially in the bipolar cells of the inner nuclear layer, which are postmitotic, fully differentiated mature neurons. Thus c-src in Xiphophorus appears to be a developmentally regulated proto-oncogene which is important for neuronal differentiation during organogenesis, but whose persistence of expression in certain terminally differentiated neurons strongly suggests a particular maintenance function for c-src in these cells as well.}, subject = {Schwertk{\"a}rpfling}, language = {en} } @article{SchartlHolsteinRobertsonetal.1989, author = {Schartl, Manfred and Holstein, Thomas and Robertson, Scott M. and Barnekow, Angelika}, title = {Preferential expression of a pp60c-src related protein tyrosine kinase activity in nerve cells of the early metazoan Hydra (Coelenterates)}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-86179}, year = {1989}, abstract = {It has been suggested that the proto-oncogene c-src plays a functional role in developing neurons, and in the mature nerve cells of higher vertebrales. The coelenterate Hydra represents tbe most primitive known organism possessing nerve cells. With Southern blot hybridizations we have demonstrated src-related sequences in Hydra. Antisera specific for the c-src gene product (pp60 c-src) of birds and mammals precipitate a protein from Hydra cell extracts with a tyrosine-specific protein kinase activity. Studies of tissues and cells fractionated from a temperature sensitive mutant of Hydra which is depleted of interstitial (including nerve) cells at tbe non-permissive temperature, have indicated the src-like kinase of Hydra to be preferentially expressed in nerve cells. The high conservation of structural features and of the expression pattern indicates a basic function for pp60c-src in neurons.}, subject = {Protein-Tyrosin-Kinasen}, language = {en} } @article{MerzFliednerLehrnbecheretal.1990, author = {Merz, H. and Fliedner, A. and Lehrnbecher, T. and Sebald, Walter and M{\"u}ller-Hermelink, H. K. and Feller, A. C.}, title = {Cytokine expression in B-cell non-Hodgkin lymphomas}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62539}, year = {1990}, abstract = {No abstract available}, subject = {Biochemie}, language = {en} } @misc{Fiala1990, author = {Fiala, Brigitte}, title = {Extrafloral nectaries versus ant-Homoptera mutualisms : a comment on Becerra and Venable}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32948}, year = {1990}, abstract = {No abstract available}, subject = {Nektarium}, language = {en} } @article{Fiala1990, author = {Fiala, Brigitte}, title = {B{\"a}ume \& Ameisen : Partnerschaften im s{\"u}dostasiatischen Regenwald}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-54741}, year = {1990}, abstract = {No abstract available}, subject = {Baum}, language = {de} } @article{Schartl1990, author = {Schartl, Manfred}, title = {Homology of melanoma-inducing loci in the genus Xiphophorus}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-61757}, year = {1990}, abstract = {Several species of the genus Xiphophorus are polymorphic for specific pigment patterns. Same of these give rise to malignant melanoma following the appropriate crossings. For one of these pattern Iod from the platyfish Xiphophorus maculatus the melanoma-inducing gene has been doned and found to encode a novel receptor tyrosine kinase, designated Xmrk. Using molecular probes from this gene in Southern blot analyses on single fish DNA preparations from 600 specimens of different populations of various species of the genus Xiphophorus and their hybrids, either with or without melanomapredisposing pattern, it was shown that all individuals contain the Xmrk gene as a proto-oncogene. It is located on the sex chromosome. All fish that carry a melanoma-predisposing locus which has been identified by Mendelian genetics contain an additional copy of Xmrk, closely linked to a specific melanophore pattern locus on the sex chromosome. The melanoma-inducing loci of the different species and populations are homologous. The additional copy of Xmrk obviously arose by a geneduplication event, thereby acquiring the oncogenic potential. The homology of the melanomainducing Iod points to a similar mechanism of tumor suppression in all feral fish populations of the different species of the genus Xiphophorus.}, subject = {Physiologische Chemie}, language = {en} } @article{DracopoliFeltquateSametal.1990, author = {Dracopoli, Nicholas C. and Feltquate, David M. and Sam, Brigitta and Schartl, Manfred}, title = {Taql and Mspl RFLPs are detected by the human 2,3-biphosphoglycerate mutase (BPGM) cDNA}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-61763}, year = {1990}, abstract = {No abstract available}, subject = {Physiologische Chemie}, language = {en} } @article{FriedenreichSchartl1990, author = {Friedenreich, Hildegard and Schartl, Manfred}, title = {Transient expression directed by homologous and heterologous promoter and enhancer sequences in fish cells}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-61774}, year = {1990}, abstract = {ln order to construct fish specific expression vectors for studies on gene regulation in vitro and in vivo a variety of heterologous enhancers and promoters from mammals and from viruses of higher vertebrate cells were tested for expression of the bacterial chloramphenicol acetyl transferase reporter gene in three teleost fish cell lines. Several viral enhancers were found to be constitutively active at high Ieveis. The human metallothionein promoter showed inducible expression in the presence of heavy metal Ions. A fish sequence was isolated that can be used as a homologous constitutively active promoter for expression of foreign genes. Using the human growth hormone gene with an active promoter in fish cells for transient expression insufficient splicing and Iack of translation were observed, pointing to limitations in the use of heterologous genes in gene transfer experiments. On the contrary, some heterologous promoters and enhancers functioned in fish c as weil as in their cell type of origin, indicating t at corresponding transcription factors are sufficient conserved between fish and human over a period of 900 million years of Independent evolution.}, subject = {Physiologische Chemie}, language = {en} } @article{ClaussWinklerLohmeyeretal.1990, author = {Clauss, Gerd and Winkler, Christoph and Lohmeyer, J{\"u}rgen and Anders, Fritz and Schartl, Manfred}, title = {Oncofetal antigen in Xiphophorus detected by monoclonal antibodies directed against melanoma-associated antigens}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-61784}, year = {1990}, abstract = {Monoclonal antlbodies (MAbs) directed against Xiphophorus melanoma cells were deve(oped and tested by lndirect immunofluorescence and Immunoperoxidase staining for reactivity with a panel of I 5 allogeneic tissues and 12 allogeneic cell llnes. The reactivity of such MAbs was restricted to melanoma cells from tumor biopsies and melanoma-derived cell lines. ln addition, all embryonie cells of all histiotypes from developmental stages later than mld·organogenesis and from corresponding short term in vitro cultures reacted with these MAbs. ln contrast, normal tissues and organs from adult fish dlsplayed no reactivity, thus implying that the melanoma-associated antigens detected by the MAbs described are oncofetal antigens.}, subject = {Physiologische Chemie}, language = {en} } @article{GesslerHameisterHenryetal.1990, author = {Gessler, Manfred and Hameister, H. and Henry, I. and Junien, C. and Braun, T. and Arnold, H. H.}, title = {The human MyoD1 (MYF3) gene maps on the short arm of chromosome 11 but is not associated with the WAGR locus or the region for the Beckwith-Wiedemann syndrome}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-59221}, year = {1990}, abstract = {The human gene encoding the myogenic determination factor myf3 (mouse MyoD1) has been mapped to the short arm of chromosome 11. Analysis of several somatic cell hybrids containing various derivatives with deletions or translocations revealed that the human MyoD (MYF3) gene is not associated with the WAGR locus at chromosomal band 11pl3 nor with the loss of the heterozygosity region at 11p15.5 related to the Beckwith-Wiedemann syndrome. Subregional mapping by in situ hybridization with an myf3 specific probe shows that the gene resides at the chromosomal band llp14, possibly at llp14.3.}, subject = {Biochemie}, language = {en} } @article{vanHeyningenBickmoreSeawrightetal.1990, author = {van Heyningen, V. and Bickmore, W. A. and Seawright, A. and Fletcher, J. M. and Maule, J. and Fekete, G. and Gessler, Manfred and Bruns, G. A. and Huerre-Jeanpierre, C. and Junien, C.}, title = {Role for the Wilms tumor gene in genital development?}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-59238}, year = {1990}, abstract = {No abstract available}, subject = {Biochemie}, language = {en} } @article{FialaMaschwitz1990, author = {Fiala, Brigitte and Maschwitz, Ulrich}, title = {Studies on the south east asian ant-plant association Crematogaster borneensis / Macaranga: adaptations of the ant partner.}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32689}, year = {1990}, abstract = {C. borneensis (Myrmicinae) lives in dose association with several myrmecophytic species of the South East Asian pioneer tree genus Macaranga (Euphorbiaceae). The ants are adapted to the plants so dosely that they do not survive away from it. The only food they utilize is provided as food bodies by the plant and honeydew from specific scale insects kept inside the hollow internodes. The anatomy of the digestive tract is also adapted to life on the host plant: the crop is very sm all and can store only minute food quantities. C. borneensis exdusively colonizes certain Macaranga species. Queens as weIl as workers are able to recognize their host plant species, probably by chemical cues. Colony founding queens swarm throughout the year, mostly during darkness. There is strong competition among queens for host plants. Queens do not carry scale insects on their nuptial flight. Worker ants are active day and night. Most of them patrol and collect food bodies on the younger parts of the host plant. An important characteristic is their deaning behaviour, which results in removal of aIl foreign objects. Even though they are rather smalI, workers respond very aggressively to certain kinds of disturbance of the host plant. The ants attack most phytophagous insects and are especially effective in killing and removing smalI, softbodied herbivores (e.g. caterpillars). They do not possess a functional sting, but apply defensive secretion and-once biting an intruder-will not let go. Their effective alarm system results in a mass attack, which provides adequate defence for the colony and the host plant. A comparison with another Crematogaster species further illustrated the special adaptations of C. borneensis to its host plant.}, language = {en} } @article{WeisingFialaRamlochetal.1990, author = {Weising, K. and Fiala, Brigitte and Ramloch, K. and Kahl, K. and Epplen, J. T.}, title = {Olingonucleotide fingerprinting in angiosperms}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-42884}, year = {1990}, abstract = {No abstract available}, language = {en} } @article{DabauvalleLoosScheer1990, author = {Dabauvalle, Marie-Christine and Loos, Karin and Scheer, Ulrich}, title = {Identification of a soluble precursor complex essential for nuclear pore assembly in vitro}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32801}, year = {1990}, abstract = {No abstract available}, language = {en} } @misc{ScheerBenavente1990, author = {Scheer, Ulrich and Benavente, Ricardo}, title = {Functional and dynamic aspects of the mammalian nucleolus}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-34269}, year = {1990}, abstract = {Nucleoli are the sites of ribosome biogenesis. Transcription of the ribosomal RNA genes as well as processing and initial packaging of their transcripts with ribosomal and non-ribosomal proteins all occur within the nucleolus in an ordered manner and under defined topological conditions. Components of the nucleolus have been localized by immunocytochemistry and their functional aspects investigated by microinjection of antibodies directed against the enzyme responsible for rDNA transcription, RNA polymerase I. The role of nascent transcripts in postmitotic formation of nucleoli will be discussed.}, language = {en} } @misc{Dandekar1990, author = {Dandekar, Thomas}, title = {Hefezellen - ein gutes Modell f{\"u}r h{\"o}here Zellen}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-29726}, year = {1990}, abstract = {No abstract available}, language = {de} } @article{DandekarSibbald1990, author = {Dandekar, Thomas and Sibbald, Peter R.}, title = {Trans-splicing of pre-mRNA is predicted to occur in a wide range of organisms including vertebrates}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-29798}, year = {1990}, abstract = {No abstract available}, language = {en} } @article{Hovestadt1990, author = {Hovestadt, Thomas}, title = {M{\"o}glichkeiten und Kriterien f{\"u}r die Bestimmung von Minimalarealen von Tierpopulationen und {\"O}kosystembest{\"a}nden}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-30150}, year = {1990}, abstract = {No abstract available}, language = {de} } @article{GesslerPoustkaCaveneeetal.1990, author = {Gessler, Manfred and Poustka, Annemarie and Cavenee, Webster and Neve, Rachael L. and Orkin, Stuart H. and Bruns, Gail A.}, title = {Homozygous deletion in Wilms tumours of a zinc-finger gene identified by chromosome jumping}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-30122}, year = {1990}, abstract = {No abstract available}, language = {en} } @article{Hovestadt1990, author = {Hovestadt, Thomas}, title = {Die Bedeutung zuf{\"a}lligen Aussterbens f{\"u}r die Naturschutzplanung}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-30136}, year = {1990}, abstract = {No abstract available}, language = {de} } @article{Linsenmair1990, author = {Linsenmair, Karl Eduard}, title = {Tropische Biodiversitat: Befunde und offene Probleme}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-78302}, year = {1990}, abstract = {During the past 50 to over 100 million years communities evolved in the tropics which attained unprecedented levels of biodiversity, strikingly represented by evergreen lowland rain forests offering home to more than 50\% of all the world's extant species. Within only some 30 years human action reduced the area covered with tropical rain forests to about half of its former size, thereby negatively affecting local and global functions of the biosphere and exterminating an unknown number of species. With an exponentially increasing rate we are throwing away our and all future generations' biological heritage. We destroy the most complicated, scientifically most interesting living systems before we have gained any knowledge of their structures ,and dynamics. To understand the particular structures and dynamics of tropical communities means in the first place to understand the causes and consequences of their ten- to more than hundredfold higher alphadiversity (as compared to temperate systems). This problem has a historical dimension and a functional side requiring answers as to the nature of the proximate mechanisms of its maintenance. My review is only concerned with the latter aspect, and its maIn emphasis is on the gaps in our knowledge. Two sets of hypotheses have been developed for explaining the high within-commUnIty diversity. (1) According to the classical concept interspecific niche competition and subsequent niche separation are the main forces determining the structure of the community. These so-called equilibrium models have been contrasted in recent times with (2) non-equilibrium models. These models do not attribute the decisive role to interspecific competition. Strong niche overlaps are presumed to be very common within species-rich communities. Continuous stochastic local disturbances are assumed to prevent the achievement of any long-term equilibrium (climax) state. Being on the right spot at the right time is regarded as most important. Whether oneor a combination of both models provide the best key for understanding the structure of a special section within a community will certainly depend on many properties of the species at debate (mobility, disr.ersal, fertility etc.). For the vast majority of tropical organisms all such information is at present unavailable. The principles governing the structure of communities is just one of the very ,basic open problems. Another very prominent question is how the qualitatively very rich, however quantitatively poor resources are distributed among the members of highly diverse guilds of consumers and decomposers. Does the scarcity rather favour generalists or specialists, are small species overrepresented, are resources more extensively used than in temperate communities? One important property is fairly well established: Populations of most tropical species seem to be very small. Since a) in very many' cases distribution range is obviously very limited, since b) predator pressure is generally assumed to be higher in the tropics and c) recent - perhaps unduely generalized - results claim abundance fluctuations in the tropics fully comparable in their dimensions to those in the temperate zone, the question arises as to how these small populations can persist for seemingly long periods of time and avoid rapid extinction. Additionally treated PoInts concern detritivore communities, plant animal Interactions, key stone groups. Saving biodiversity in general and the tropical species and community richness in particular is one of the most urgent tasks of our generation, and biologists have to play a still more prominent role in this extremely important endeavor than they have in the past decades.}, subject = {Zoologie}, language = {de} } @article{BarnekowJahnSchartl1990, author = {Barnekow, Angelika and Jahn, Reinhard and Schartl, Manfred}, title = {Synaptophysin: a substrate for the protein tyrosine kinase pp60c-src in intact synaptic vesicles}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-86168}, year = {1990}, abstract = {Expression of pp60 c-src, the first well defined proto-oncogene product, is developmentally regulated and tissue-specific, with neuronal tissues displaying high amounts of the c-src encoded pp60 c-src kinase activity. In the central nervous system pp60 s-src is preferentially expressed in regions characterized by a high content of grey matter and elevated density of nerve terminals. In this study we show for the first time a direct interaction between pp60 c-src and synaptophysin as a physiological target protein in neurons by demonstrating that endogenous pp60 c-src is able to phosphorylate synaptophysin (p38). p38 is a major constituent of the synaptic vesicle membrane protein and is thought to play a key role in the exocytosis of small synaptic vesicles and possibly small clear vesicles in neuroendocrine cells.}, subject = {Synaptophysin}, language = {en} } @article{SchartlNandaSchluppetal.1990, author = {Schartl, Manfred and Nanda, Indrajit and Schlupp, Ingo and Parzefall, Jakob and Schmid, Michael and Epplen, J{\"o}rg T.}, title = {Genetic variation in the clonal vertebrate Poecilia formosa is limited to few truly hypervariable loci}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-86359}, year = {1990}, abstract = {No abstract available.}, subject = {Amazon Molly}, language = {en} } @article{FialaMebert1990, author = {Fiala, Brigitte and Mebert, M.}, title = {Partnerschaft f{\"u}rs {\"U}berleben. Ameisenb{\"a}ume im tropischen Regenwald.}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-42900}, year = {1990}, abstract = {No abstract available}, language = {de} } @incollection{Fiala1990, author = {Fiala, Brigitte}, title = {Die Ameisenpflanzen der Gattung Macaranga (Euphorbiaceae) - verschiedene Stufen der Pflanzen-Ameisen-Beziehungen}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-42914}, publisher = {Universit{\"a}t W{\"u}rzburg}, year = {1990}, abstract = {No abstract available}, language = {de} } @article{SchartlSchartl1990, author = {Schartl, Angelika and Schartl, Manfred}, title = {Genes and cancer: Molecular biology of the melanoma oncogene of Xiphophorus}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-72670}, year = {1990}, abstract = {No abstract available.}, subject = {Schwertk{\"a}rpfling}, language = {en} } @inproceedings{Mahsberg1990, author = {Mahsberg, Dieter}, title = {Brood care and family cohesion in the tropical scorpion Pandinus imperator (Koch) (Scorpiones: Scorpionidae)}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-45776}, year = {1990}, abstract = {Pandinus imperator is a forest dweller of tropical West Africa. In the field, lobserved aggregations of up to 15 individuals. In the laboratory, mixed age groups of related and also unrelated animals lived jointly in terraria rarely showing within-group aggression or cannibalism. Brood-caring behavior of the mother influenced growth rate and survival probability of the young. With birth, mothers became very aggressive. To study family cohesion in Pandinus, experiments with family groups were conducted. Siblings aggregated around their mother. In choice experiments with two family groups, mothers were placed in enclosures that only the young were able to enter or to leave. Second instars significantly preferred the enclosure containing their own mother. Aggression among unrelated young of the same age was not observed. Feeding experiments studied the possible advantages of long-Iasting group living with regard to enhanced success in prey capture and its effect on growth of the young. Even groups of second instars were unable to subdue large prey on their own. Sibling groups with their mother removed suffered high mortality due to starvation and cannibalism compared to groups with mothers present. Here, young grew significantly faster: they shared the prey that only the mother was able to kill and dismember. Pandinus imperator has to be considered an intermediate subsocial scorpion.}, subject = {Skorpion}, language = {en} } @inproceedings{FialaMaschwitzTho1991, author = {Fiala, Brigitte and Maschwitz, Ulrich and Tho, Yow Pong}, title = {The association between Macaranga trees and ants in South-east Asia}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-54752}, year = {1991}, abstract = {No abstract available}, subject = {Macaranga}, language = {de} } @article{LehrnbecherMerzSebaldetal.1991, author = {Lehrnbecher, T. and Merz, H. and Sebald, Walter and Poot, M.}, title = {Interleukin 4 drives phytohemagglutinin-activated T cells through several cell cycles: no synergism between interleukin 2 and interleukin 4}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62491}, year = {1991}, abstract = {Cell kinetic studies of T cells stimulated with the interleukin 2 (11-2), D-4, or both lymphokines were performed with conventional [3H] thymidine incorporation and with the bivariate BrdU/Hoechst technique. 11-2 and 11-4 are able to drive phytohemagglutininactivated T cells through more than one cell cycle. Neither synergistic nor inhibitory efl'ect on T -cell proliferationwas seen for the stimulation with both 11-2 and 11-4 as compared with the effect ofll-2 alone. The quantitative data ofthe cell cycle distribution ofphytohemagglutininactivated T cells suggestthat the population ofll-4-responsive cells is at least an overlapping population, if not a real subset of the ·population of the 11-2-responsive cells.}, subject = {Biochemie}, language = {en} } @article{MerzFliednerOrschescheketal.1991, author = {Merz, H. and Fliedner, A. and Orscheschek, K. and Binder, T. and Sebald, Walter and M{\"u}ller-Hermelink, H. K. and Feller, A. C.}, title = {Cytokine expression in T-cell lymphomas and Hodgkin's disease. Its possible implication in autocrine or paracrine production as a potential basis for neoplastic growth}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62483}, year = {1991}, abstract = {No abstract available}, subject = {Biochemie}, language = {en} }