@article{RubtsovChernovGorboulevetal.1985,
  author    = {Rubtsov, P. M. and Chernov, V. G. and Gorboulev, Valentin G. and Parsadanyan, A. S. and Sverdlova, P. S. and Chupeeva, V. V. and Golova, Yu. B. and Batchikova, N. V. and Zvirblis, G. S. and Skryabin, K. G. and Bayev, A. A.},
  title     = {Genetic engineering of peptide hormones},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-46964},
  year      = {1985},
  abstract  = {Peptide and polypeptide hormones represent an extensive group of biologically active compounds of important significance for medicine and agriculture. In recent years genetic engineering methods have been used to create strains of microorganisms synthesizing eukaryotic proteins, including hormones and their precursors. The first stage of such developments is the isolation of DNA coding the des~red product. We have accomplished the cloning of the cDNA of a number of polypeptide and peptide hormones of the pituitary of man and domestic animals. The model gene of human calcitonin has also been synthesized and cloned. The obtained genes are being used to develop methods for the microbiological synthesis of human and animal-hormones.},
  language  = {en}
}
@article{RubtsovOganessyanGorboulevetal.1988,
  author    = {Rubtsov, P. M. and Oganessyan, R. G. and Gorboulev, Valentin G. and Skryabin, K. G. and Bayev, A. A.},
  title     = {Genetic engineering of peptide hormones : II. Possible polymorphism of preprolactin in cattle. Data of molecular cloning},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-46975},
  year      = {1988},
  abstract  = {Primary structure is determined of an insertion of a clone isolated from the library of hypophyseal cDNA of cattle by hybridization with a probe specific for prolactin. Analysis of nucleotide sequences showed that in the process of cloning, reorganization occurred in structure of preprolactin cDNA, including an inversion of the 5'-terminal and deletion of the central section of cDNA. Nevertheless, from structure of cDNA, nucleotide sequences can be deduced of extended 5'- and 3'-terminal sections of preprolactin mRNA in cattle with lengths of 257 and 551 nucleotide residues, respectively. When these sequences are compared to those established previously, some differences were found in primary structure. The most important of them is the presence of an additional codon which codes alanine at the position (-22) of the signal peptide. It is suggested that heterogeneity of preprolactin mRNA of cattle in the section coding the signal peptide is the result of alternative splicing, as was shown for preprolactin mRNA in rats.},
  language  = {en}
}