@article{OliAbdelmohsenHentscheletal.2014,
  author    = {Oli, Swarna and Abdelmohsen, Usama Ramadan and Hentschel, Ute and Schirmeister, Tanja},
  title     = {Identification of Plakortide E from the Caribbean Sponge Plakortis halichondroides as a Trypanocidal Protease Inhibitor using Bioactivity-Guided Fractionation},
  series = {MARINE DRUGS},
  volume    = {12},
  journal   = {MARINE DRUGS},
  number    = {5},
  issn      = {1660-3397},
  doi       = {10.3390/md12052614},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-116536},
  pages     = {2614-2622},
  year      = {2014},
  abstract  = {In this paper, we report new protease inhibitory activity of plakortide E towards cathepsins and cathepsin-like parasitic proteases. We further report on its anti-parasitic activity against Trypanosoma brucei with an IC50 value of 5 mu M and without cytotoxic effects against J774.1 macrophages at 100 mu M concentration. Plakortide E was isolated from the sponge Plakortis halichondroides using enzyme assay-guided fractionation and identified by NMR spectroscopy and mass spectrometry. Furthermore, enzyme kinetic studies confirmed plakortide E as a non-competitive, slowly-binding, reversible inhibitor of rhodesain.},
  language  = {en}
}