@article{WeberOsbornFrankeetal.1977, author = {Weber, Klaus and Osborn, Mary and Franke, Werner W. and Seib, Erinita and Scheer, Ulrich and Herth, Werner}, title = {Identification of microtubular structures in diverse plant and animal cells by immunological cross-reaction revealed in immunofluorescence microscopy using antibodies against tubulin from porcine brain}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-41383}, year = {1977}, abstract = {Antibody against tubulin from porcine brain was used to evaluate the immunological cross reactivity of tubulin from a variety of animal and plant cells. Indirect immunofluorescence microscopy revealed microtubule-containing structures including cytoplasmic microtubules, spindle microtubules, cilia and fIagella. Thus tubulin from diverse species of both mammals and plants show immunological cross-reactivity with tubulin from porcine brain. Results obtained by immunofluorescence microscopy are whenever possible compared with previously known ultrastructural results obtained by electron microscopy.}, subject = {Cytologie}, language = {en} } @article{SpringKrohneFrankeetal.1976, author = {Spring, Herbert and Krohne, Georg and Franke, Werner W. and Scheer, Ulrich and Trendelenburg, Michael F.}, title = {Homogeneity and heterogeneity of sizes of transcriptional units and spacer regions in nucleolar genes of Acetabularia}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-41398}, year = {1976}, abstract = {The arrangement of genes of precursor molecules for ribosomal RNA (pre-rRNA) in primary nuclei from two green algae species, Acetabularia mediterranea and A. major, has been analyzed in an electron microscope study. The pattern of transcriptional units in individual strands of nucleolar chromatin was investigated using spread and positively stained preparations. The rDNA pattern is not uniform but differs in different strands. The predominant type of nucleolar chromatin exhibits a high degree of homogeneity in the sequence of matrix units (intercepts covered with fibrilst hat contain the pre-rRNA) and fibril-free spacer intercepts. Substantial differences, however, are observed between the patterns in different strands. In addition, there is evidence in some strands for intraaxial heterogeneity of both spacer and matrix units. The following major types can be distinguished: type la, ca. 2 micrometer long matrix units, extremely short spacer intercepts in A. mediterranea (ca. 1 micrometer long ones in A. major), completely homogeneous distribution; type Ib, as type la but with intercalated, isolated, significantly shorter and/or longer matrix units; type lIa, matrix unit sizes as in type la, but much longer spacer intercepts, high degree of homogeneity; type Ill, largely heterogeneous arrangements of matrix and spacer units of varying sizes. The matrix unit data are compared with the sizes of pre-rRNA as determined by polyacrylamide gelelectrophoresis under denaturing and non-denaturing conditions. The findings are discussed in relation to recent observations in amphibia and insects and with respect to current concepts of the species-specificity of rDNA arrangements.}, language = {en} } @article{FrankeJaraschHerthetal.1975, author = {Franke, Werner W. and Jarasch, Ernst-Dieter and Herth, Werner and Scheer, Ulrich and Zerban, Heide}, title = {Cytology : general and molecular cytology}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-41458}, year = {1975}, abstract = {The present review discusses some general aspects of membrane structure and problems of membrane isolation and membrane biochemistry, with particular focus on the endoplasmic reticulum.}, subject = {Botanik}, language = {en} } @book{KartenbeckZentgrafScheeretal.1971, author = {Kartenbeck, J. and Zentgraf, H. and Scheer, Ulrich and Franke, Werner W.}, title = {The nuclear envelope in freeze-etching}, isbn = {3-540-05538-X}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-40534}, publisher = {Universit{\"a}t W{\"u}rzburg}, year = {1971}, abstract = {No abstract available}, subject = {Anatomie}, language = {en} } @article{KnechtScheer1972, author = {Knecht, Sigrid and Scheer, Ulrich}, title = {Die Liste der Vogelarten von S. Miguel (Azoren) des Gaspar Fructuoso (gestorben 1591)}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-41402}, year = {1972}, abstract = {No abstract available}, language = {de} } @misc{ReimerRaskaTanetal.1987, author = {Reimer, Georg and Raska, Ivan and Tan, Eng M. and Scheer, Ulrich}, title = {Human autoantibodies: probes for nucleolus structure and function}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-41410}, year = {1987}, abstract = {No abstract available}, language = {en} } @article{ScheerFrankeTrendelenburgetal.1976, author = {Scheer, Ulrich and Franke, Werner W. and Trendelenburg, Michael F. and Spring, Herbert}, title = {Classification of loops of lampbrush chromosomes according to the arrangement of transcriptional complexes}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32822}, year = {1976}, abstract = {The arrangement of transcriptional units in the loops of lampbrush chromosomes from oocyte nuclei of urodele amphibia and from primary nuclei of the green alga Acetabularia have been studied in the electron microscope using spread preparations. Loops with different patterns of arrangement of matrix units (i.e. to a first approximation, transcriptional units) can be distinguished: (i) loops consisting of one active transcriptional unit; (ii) loops containing one active transcriptional unit plus additional fibril-free, i.e. apparently untranscribed, intercepts that may include 'spacer' regions; (iii) loops containing two or more transcriptional units arranged in identical or changing polarities, with or without interspersed apparent spacer regions. Morphological details of the transcriptional complexes are described. The observations are not compatible with the concept that one loop reflects one and only one transcriptional unit but, rather, lead to a classification of loop types according to the arrangement of their transcriptional units. We propose that the lampbrush chromosome loop can represent a unit for the coordinate transcription of either one gene or a set of several (different) genes.}, language = {en} } @article{FrankeSpringScheeretal.1975, author = {Franke, Werner W. and Spring, Herbert and Scheer, Ulrich and Zerban, Heide}, title = {Growth of the nuclear envelope in the vegetative phase of the green alga Acetabularia. Evidence for assembly from membrane components synthesized in the cytoplasm.}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32403}, year = {1975}, abstract = {No abstract available}, language = {en} } @article{ScheerFrankeTrendelenburg1975, author = {Scheer, Ulrich and Franke, Werner W. and Trendelenburg, Michael F.}, title = {Effects of actinomycin D on the association of newly formed ribonucleoproteins with the cistrons of ribosomal RNA in Triturus oocytes}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32383}, year = {1975}, abstract = {No abstract available}, language = {en} } @article{FrankeKartenbeckZentgrafetal.1971, author = {Franke, Werner W. and Kartenbeck, J{\"u}rgen and Zentgraf, Hanswalter and Scheer, Ulrich and Falk, Heinz}, title = {Membrane-to-membrane cross-bridges. A means to orientation and interaction of membrane faces}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32122}, year = {1971}, abstract = {No abstract available}, language = {en} } @article{FrankeScheerFritsch1972, author = {Franke, Werner W. and Scheer, Ulrich and Fritsch, Hansj{\"o}rg}, title = {Intranuclear and cytoplasmic annulate lamellae in plant cells}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32148}, year = {1972}, abstract = {No abstract available}, language = {en} } @article{Scheer1970, author = {Scheer, Ulrich}, title = {The ultrastructure of the nuclear envelope of amphibian oocytes: a reinvestigation. III. Actinomycin D-induced decrease in central granules within the pores.}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32110}, year = {1970}, abstract = {No abstract available}, language = {en} } @article{WilkenKossnerSenecaletal.1993, author = {Wilken, Norbert and Kossner, Ursula and Sen{\´e}cal, Jean-Luc and Scheer, Ulrich and Dabauvalle, Marie-Christine}, title = {Nup180, a novel nuclear pore complex protein localizing to the cytoplasmic ring and associated fibrils}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32049}, year = {1993}, abstract = {No abstract available}, language = {en} } @article{DabauvalleLoosMerkertetal.1991, author = {Dabauvalle, Marie-Christine and Loos, Karin and Merkert, Hilde and Scheer, Ulrich}, title = {Spontaneous assembly of pore complex-containing membranes ("Annulate lamellae") in Xenopus egg extract in the absence of chromatin}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32797}, year = {1991}, abstract = {No abstract available}, language = {en} } @article{ScheerTrendelenburgFranke1976, author = {Scheer, Ulrich and Trendelenburg, Michael F. and Franke, Werner W.}, title = {Regulation of transcription of genes of ribosomal RNA during amphibian oogenesis: a biochemical and morphological study}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32814}, year = {1976}, abstract = {Natural changes in the transcription of rRNA genes were studied in nucleoli from three oogenic stages of the newt Triturus alpestris with electron microscope, autoradiographic, and biochemical techniques. From determinations of the uridine triphosphate pool sizes and [3H]uridine uptake, phosphorylation, and incorporation into 28S and 18S rRNAs in vivo it was estimated that the rate of rRNA synthesis was about 0.01\% in previtellogenic oocytes and 13\% in mature oocytes when compared to midvitellogenesis. Spread preparations of nucleoli showed significant morphological changes in the transcriptional complexes. The total number of lateral fibrils, i.e., ribonucleoproteins containing the nascent rRNA precursor, were drastically decreased in stages of reduced synthetic activity. This indicates that rRNA synthesis is regulated primarily at the level of transcription. The resulting patterns of fibril coverage of the nucleolar chromatin axes revealed a marked heterogeneity. On the same nucleolar axis occurred matrix units that were completely devoid of lateral fibrils, matrix units that were almost fully covered with lateral fibrils, and various forms of matrix units with a range of lateral fibril densities intermediate between the two extremes. Granular particles that were tentatively identified as RNA polymerase molecules were not restricted to the transcription l complexes. They were observed, although less regularly and separated by greater distances, in untranscribed spacer regions as well as in untranscribed gene intercepts. The results show that the pattern of transcriptional control of rRNA genes differs widely in different genes, even in the same genetic unit.}, language = {en} } @article{Scheer1975, author = {Scheer, Ulrich}, title = {The rifamycin derivative AF/013 is cytolytic}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32429}, year = {1975}, abstract = {No abstract available}, language = {en} } @inproceedings{TrendelenburgSpringScheeretal.1974, author = {Trendelenburg, Michael F. and Spring, Herbert and Scheer, Ulrich and Franke, Werner W.}, title = {Morphology of nucleolar cistrons in a plant cell, Acetabularia mediterranea}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32213}, year = {1974}, abstract = {The structural organization of transcriptionally active DNA that contains cistrons for precursor molecules of ribosomal RNA is described in positively stained spread preparations from nuclei and nucleoli isolated from the green alga, Acetabularia mediterranea Lmx. These nuclei contain large aggregates of nucleolar subunits in which fibril-covered regions, the putative active cistrons for precursors of ribosomal RNA, alternate with fibril-free intercepts, the "spacers". The length distribution of the different intercepts of this DNA is given, and the pattern is compared with those shown in animal cell systems. The data are discussed in relation to problems of transcription and of amplification of ribosomal RNA genes.}, language = {en} } @article{ScheerFranke1972, author = {Scheer, Ulrich and Franke, Werner W.}, title = {Annulate lamellae in plant cells: formation during microsporogenesis and pollen development in Canna generalis Bailey}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32160}, year = {1972}, abstract = {The occurrence of stacked annulate tamellae is documented for a plant cell system, namely for pollen mother cells and developing pollen grains of Canna generalis. Their structural subarchiteeture and relationship to endoplasmie reticulum (ER) and nuclear envelope cisternae is described in detail. The results demonstrate structural homology between plant and animal annulate lamellae and are compatible with, though do not prove, the view that annulate lamcllar cisternae may originate as a degenerative form of endoplasmic retieulum.}, language = {en} } @article{EckertFrankeScheer1975, author = {Eckert, W. A. and Franke, Werner W. and Scheer, Ulrich}, title = {Nucleocytoplasmic translocation of RNA in Tetrahymena pyriformis and its inhibition by actinomycin D and cycloheximide}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32399}, year = {1975}, abstract = {No abstract available}, language = {en} } @article{SpringScheerFrankeetal.1975, author = {Spring, Herbert and Scheer, Ulrich and Franke, Werner W. and Trendelenburg, Michael F.}, title = {Lampbrush type chromosomes in the primary nucleus of the green alga Acetabularia mediterranea}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32370}, year = {1975}, abstract = {No abstract available}, language = {en} }