@article{JacobsBockSchuchetal.2012,
  author    = {Jacobs, Graeme and Bock, Stefanie and Schuch, Anita and Moschall, Rebecca and Schrom, Eva-Maria and Zahn, Juliane and Reuter, Christian and Preiser, Wolfgang and Rethwilm, Axel and Engelbrecht, Susan and Krekau, Thomas and Bodem, Jochen},
  title     = {Construction of a high titer Infectious HIV-1 subtype C proviral clone from South Africa},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-76340},
  year      = {2012},
  abstract  = {The Human Immunodeficiency Virus type 1 (HIV-1) subtype C is currently the predominant subtype worldwide. Cell culture studies of Sub-Saharan African subtype C proviral plasmids are hampered by the low replication capacity of the resulting viruses, although viral loads in subtype C infected patients are as high as those from patients with subtype B. Here, we describe the sequencing and construction of a new HIV-1 subtype C proviral clone (pZAC), replicating more than one order of magnitude better than the previous subtype C plasmids. We identify the env-region for being the determinant for the higher viral titers and the pZAC Env to be M-tropic. This higher replication capacity does not lead to a higher cytotoxicity compared to previously described subtype C viruses. In addition, the pZAC Vpu is also shown to be able to down-regulate CD4, but fails to fully counteract CD317.},
  subject      = {HIV},
  language  = {en}
}