@article{SagelsdorffLutzSchlatter1983,
  author    = {Sagelsdorff, P. and Lutz, Werner K. and Schlatter, C.},
  title     = {The relevance of covalent binding to mouse liver DNA to the carcinogenic action of hexachlorocyclohexane isomers},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-61039},
  year      = {1983},
  abstract  = {[\(^3\)H]Hexachlorocyclohexane (HCH) was synthesized by chlorination of [\(^3\)H]benzene prepared by catalytic tritiation of benzene with tritiated water. The isomers of HCH were separated by adsorption chromatography on silica gel. In order to determine the covalent binding to DNA, [\(^3\)H]HCH was administered to male mice by oral gavage, and liver DNA was isolated via cbromatin. The specific radioactivity of the DNA was nonnalized by the dose administered and expressed in the molar units of the Covalent binding index, CBI = DNA damage/dose = (\(\mu\)mol bound HCH/mol DNA nucleotide)/(mmol HCH administered/kg body weight). CBI values of - 0.2 were found 10 h after the administration of alpha- and gamma-HCH. Enzymatic digestion of the DNA to the nucleosides and h.p.l.c. analysis revealed that - 40\% of the radioactivity co-migrated with the natural nucleosides. At elution volumes known to contain the more lipophilic carcinogen-nucleoside adducts, - 10\% of the radioactivity could be detected. The remaining 50\% of th,e radioactivity eluted with the front, representing a mixture of oligonucleotide- HCH adducts and/or hydrophilic degradation products which were strongly bot not covalently associated with intact DNA. Therefore, a true CBI of 0.02-0.1 must be expected both for alpha- and gamma-HCH. This CBI is by a factor of 10\(^5\) -10\(^6\) below the value found with the strongest DNAbinding carcinogens like aflatoxin B1 or dimethylnitrosamine and is unlikely to be decisive for the liver tumor induction in mice because of the foUowing additional findings: (i) both isomers gave rise to similar Ievels of DNA darnage although the alpha-isomer is a much morepotent tumor inducer. This similarity was seen not only at the time of m{\"a}ximum binding but up to 10 days after oral administration; (ii) three mouse strains with apparently different susceptibility to tumor induction by gamma-HCH could not be distinguished with respect to DNA binding; (iii) the level of DNA binding of alpha-HCH (CBI = 0.02-0.1) is more than three orders of magnitude lower than would be expected if the mechanism of tumor induction was by genotoxicity mediated by DNAbinding. For a preliminary investigation on a potential stimulatory effect on liver DN A replication and ceU division, [\(^{14}\)]thymidine was admlnistered i.p. 3.5 h before sacrifice of the [\(^3\)H]HCH-treated mice. The alpha-isomer was found to be more potent than the gamma-isomer in this respect. Taken together, our data allow the conclusion that the non- mutational processes must be more important for the carcinogenicity of HCH.},
  subject      = {Toxikologie},
  language  = {en}
}
@article{MeierBratschiLutzSchlatter1983,
  author    = {Meier-Bratschi, A. and Lutz, Werner K. and Schlatter, C.},
  title     = {Methylation of liver DNA of rat and mouse by N-nitrosodimethylamine formed in vivo from dimethylamine and nitrite},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-61052},
  year      = {1983},
  abstract  = {The extent of formation of N-nitrosodimethylaminc {NDMA) in the stomachs of rats and mice after sirnultancous oral administration of [\(^{14}\)C]dimethylamine and potassium nitrite was determined by measuring the methylation of liver DNA. With doses of around 1 mg dimethylamine hydrochloride/ kg body weight and 50 mg potassium nitrite/kg body weight. 0,8 \% of the amine was nitrosated on average. The individual fluctuations ranged from 0.2 to 1.30\% in the rat and from 0.2 to 1.9\% in the mouse. Simultaneous administration of 50 mg sodium ascorbate (vitamin Cl/kg body weight inhibited the nitrosation by ahout 80\% while 50 mg \(\alpha\)-tocopherol acetate [Vitamin E)/kg body weight reduced the nitrosation by about a half. Assuming similar kinctics and conditions of nitrosation in rats and man. a comparison of the formation of NDMA in vivo from dietary dimethylamine and nitrite with the estimated human uptake of preformed N DMA revealed that in vitro formation in the stomach of man is probably negligible.},
  subject      = {Toxikologie},
  language  = {en}
}