@article{WegenerKarsaiPollaketal.2013,
  author    = {Wegener, Christian and Karsai, Gergely and Poll{\´a}k, Edit and Wacker, Matthias and V{\"o}mel, Matthias and Selcho, Mareike and Berta, Gergely and Nachman, Ronald J. and Isaac, R. Elwyn and Moln{\´a}r, L{\´a}szl{\´o}},
  title     = {Diverse in- and output polarities and high complexity of local synaptic and non-synaptic signaling within a chemically defined class of peptidergic Drosophila neurons},
  series = {Frontiers in Neural Circuits},
  journal   = {Frontiers in Neural Circuits},
  doi       = {10.3389/fncir.2013.00127},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-96914},
  year      = {2013},
  abstract  = {Peptidergic neurons are not easily integrated into current connectomics concepts, since their peptide messages can be distributed via non-synaptic paracrine signaling or volume transmission. Moreover, the polarity of peptidergic interneurons in terms of in- and out-put sites can be hard to predict and is very little explored. We describe in detail the morphology and the subcellular distribution of fluorescent vesicle/dendrite markers in CCAP neurons (NCCAP), a well defined set of peptidergic neurons in the Drosophila larva. NCCAP can be divided into five morphologically distinct subsets. In contrast to other subsets, serial homologous interneurons in the ventral ganglion show a mixed localization of in- and output markers along ventral neurites that defy a classification as dendritic or axonal compartments. Ultrastructurally, these neurites contain both pre- and postsynaptic sites preferably at varicosities. A significant portion of the synaptic events are due to reciprocal synapses. Peptides are mostly non-synaptically or parasynaptically released, and dense-core vesicles and synaptic vesicle pools are typically well separated. The responsiveness of the NCCAP to ecdysis-triggering hormone may be at least partly dependent on a tonic synaptic inhibition, and is independent of ecdysteroids. Our results reveal a remarkable variety and complexity of local synaptic circuitry within a chemically defined set of peptidergic neurons. Synaptic transmitter signaling as well as peptidergic paracrine signaling and volume transmission from varicosities can be main signaling modes of peptidergic interneurons depending on the subcellular region. The possibility of region-specific variable signaling modes should be taken into account in connectomic studies that aim to dissect the circuitry underlying insect behavior and physiology, in which peptidergic neurons act as important regulators.},
  language  = {en}
}
@article{LyutovaSelchoPfeufferetal.2019,
  author    = {Lyutova, Radostina and Selcho, Mareike and Pfeuffer, Maximilian and Segebarth, Dennis and Habenstein, Jens and Rohwedder, Astrid and Frantzmann, Felix and Wegener, Christian and Thum, Andreas S. and Pauls, Dennis},
  title     = {Reward signaling in a recurrent circuit of dopaminergic neurons and peptidergic Kenyon cells},
  series = {Nature Communications},
  volume    = {10},
  journal   = {Nature Communications},
  doi       = {10.1038/s41467-019-11092-1},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-202161},
  pages     = {3097},
  year      = {2019},
  abstract  = {Dopaminergic neurons in the brain of the Drosophila larva play a key role in mediating reward information to the mushroom bodies during appetitive olfactory learning and memory. Using optogenetic activation of Kenyon cells we provide evidence that recurrent signaling exists between Kenyon cells and dopaminergic neurons of the primary protocerebral anterior (pPAM) cluster. Optogenetic activation of Kenyon cells paired with odor stimulation is sufficient to induce appetitive memory. Simultaneous impairment of the dopaminergic pPAM neurons abolishes appetitive memory expression. Thus, we argue that dopaminergic pPAM neurons mediate reward information to the Kenyon cells, and in turn receive feedback from Kenyon cells. We further show that this feedback signaling is dependent on short neuropeptide F, but not on acetylcholine known to be important for odor-shock memories in adult flies. Our data suggest that recurrent signaling routes within the larval mushroom body circuitry may represent a mechanism subserving memory stabilization.},
  language  = {en}
}
@article{SelchoMillanPalaciosMunozetal.2017,
  author    = {Selcho, Mareike and Mill{\´a}n, Carola and Palacios-Mu{\~n}oz, Angelina and Ruf, Franziska and Ubillo, Lilian and Chen, Jiangtian and Bergmann, Gregor and Ito, Chihiro and Silva, Valeria and Wegener, Christian and Ewer, John},
  title     = {Central and peripheral clocks are coupled by a neuropeptide pathway in Drosophila},
  series = {Nature Communications},
  volume    = {8},
  journal   = {Nature Communications},
  number    = {15563},
  doi       = {10.1038/ncomms15563},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-170831},
  year      = {2017},
  abstract  = {Animal circadian clocks consist of central and peripheral pacemakers, which are coordinated to produce daily rhythms in physiology and behaviour. Despite its importance for optimal performance and health, the mechanism of clock coordination is poorly understood. Here we dissect the pathway through which the circadian clock of Drosophila imposes daily rhythmicity to the pattern of adult emergence. Rhythmicity depends on the coupling between the brain clock and a peripheral clock in the prothoracic gland (PG), which produces the steroid hormone, ecdysone. Time information from the central clock is transmitted via the neuropeptide, sNPF, to non-clock neurons that produce the neuropeptide, PTTH. These secretory neurons then forward time information to the PG clock. We also show that the central clock exerts a dominant role on the peripheral clock. This use of two coupled clocks could serve as a paradigm to understand how daily steroid hormone rhythms are generated in animals.},
  language  = {en}
}