@article{TucaBernardellideMattosFunketal.2022,
  author    = {Tuca, Alexandru-Cristian and Bernardelli de Mattos, Ives and Funk, Martin and Winter, Raimund and Palackic, Alen and Groeber-Becker, Florian and Kruse, Daniel and Kukla, Fabian and Lemarchand, Thomas and Kamolz, Lars-Peter},
  title     = {Orchestrating the dermal/epidermal tissue ratio during wound healing by controlling the moisture content},
  series = {Biomedicines},
  volume    = {10},
  journal   = {Biomedicines},
  number    = {6},
  issn      = {2227-9059},
  doi       = {10.3390/biomedicines10061286},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-275115},
  year      = {2022},
  abstract  = {A balanced and moist wound environment and surface increases the effect of various growth factors, cytokines, and chemokines, stimulating cell growth and wound healing. Considering this fact, we tested in vitro and in vivo water evaporation rates from the cellulose dressing epicite\(^{hydro}\) when combined with different secondary dressings as well as the resulting wound healing efficacy in a porcine donor site model. The aim of this study was to evaluate how the different rates of water evaporation affected wound healing efficacy. To this end, epicite\(^{hydro}\) primary dressing, in combination with different secondary dressing materials (cotton gauze, JELONET\(^◊\), AQUACEL\(^®\) Extra\(^™\), and OPSITE\(^◊\) Flexifix), was placed on 3 × 3 cm-sized dermatome wounds with a depth of 1.2 mm on the flanks of domestic pigs. The healing process was analyzed histologically and quantified by morphometry. High water evaporation rates by using the correct secondary dressing, such as cotton gauze, favored a better re-epithelialization in comparison with the low water evaporation resulting from an occlusive secondary dressing, which favored the formation of a new and intact dermal tissue that nearly fully replaced all the dermis that was removed during wounding. This newly available evidence may be of great benefit to clinical wound management.},
  language  = {en}
}
@article{WeigelMalkmusWeigeletal.2022,
  author    = {Weigel, Tobias and Malkmus, Christoph and Weigel, Verena and Wußmann, Maximiliane and Berger, Constantin and Brennecke, Julian and Groeber-Becker, Florian and Hansmann, Jan},
  title     = {Fully Synthetic 3D Fibrous Scaffolds for Stromal Tissues—Replacement of Animal-Derived Scaffold Materials Demonstrated by Multilayered Skin},
  series = {Advanced Materials},
  volume    = {34},
  journal   = {Advanced Materials},
  number    = {10},
  doi       = {10.1002/adma.202106780},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-276403},
  year      = {2022},
  abstract  = {The extracellular matrix (ECM) of soft tissues in vivo has remarkable biological and structural properties. Thereby, the ECM provides mechanical stability while it still can be rearranged via cellular remodeling during tissue maturation or healing processes. However, modern synthetic alternatives fail to provide these key features among basic properties. Synthetic matrices are usually completely degraded or are inert regarding cellular remodeling. Based on a refined electrospinning process, a method is developed to generate synthetic scaffolds with highly porous fibrous structures and enhanced fiber-to-fiber distances. Since this approach allows for cell migration, matrix remodeling, and ECM synthesis, the scaffold provides an ideal platform for the generation of soft tissue equivalents. Using this matrix, an electrospun-based multilayered skin equivalent composed of a stratified epidermis, a dermal compartment, and a subcutis is able to be generated without the use of animal matrix components. The extension of classical dense electrospun scaffolds with high porosities and motile fibers generates a fully synthetic and defined alternative to collagen-gel-based tissue models and is a promising system for the construction of tissue equivalents as in vitro models or in vivo implants.},
  language  = {en}
}
@article{ReuterHaufImdahletal.2023,
  author    = {Reuter, Christian and Hauf, Laura and Imdahl, Fabian and Sen, Rituparno and Vafadarnejad, Ehsan and Fey, Philipp and Finger, Tamara and Jones, Nicola G. and Walles, Heike and Barquist, Lars and Saliba, Antoine-Emmanuel and Groeber-Becker, Florian and Engstler, Markus},
  title     = {Vector-borne Trypanosoma brucei parasites develop in artificial human skin and persist as skin tissue forms},
  series = {Nature Communications},
  volume    = {14},
  journal   = {Nature Communications},
  doi       = {10.1038/s41467-023-43437-2},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-358142},
  year      = {2023},
  abstract  = {Transmission of Trypanosoma brucei by tsetse flies involves the deposition of the cell cycle-arrested metacyclic life cycle stage into mammalian skin at the site of the fly's bite. We introduce an advanced human skin equivalent and use tsetse flies to naturally infect the skin with trypanosomes. We detail the chronological order of the parasites' development in the skin by single-cell RNA sequencing and find a rapid activation of metacyclic trypanosomes and differentiation to proliferative parasites. Here we show that after the establishment of a proliferative population, the parasites enter a reversible quiescent state characterized by slow replication and a strongly reduced metabolism. We term these quiescent trypanosomes skin tissue forms, a parasite population that may play an important role in maintaining the infection over long time periods and in asymptomatic infected individuals.},
  language  = {en}
}