@phdthesis{Pinzner2021,
  author    = {Pinzner, Florian},
  title     = {Vicinal and Double Chemoselective Biofunctionalization of Polyoxazolines},
  doi       = {10.25972/OPUS-22975},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-229758},
  school      = {Universit{\"a}t W{\"u}rzburg},
  year      = {2021},
  abstract  = {In this work, a toolbox was provided to create three-component polymer conjugates with a defined architecture, designed to bear different biocomponents that can interact with larger biological systems in biomacromolecular recognition experiments. The target architecture is the attachment of two biomolecule 'arms' to the alpha telechelic end point of a polymer and fixating the conjugate to the gold surface of SAW and SPR sensor chips with the polymer's other omega chain end. This specific design of a conjugate will be implemented by using a strategy to yield novel double alpha as well as omega telechelic functionalized POx and the success of all cascade reaction steps leading to the final conjugation product will be proven through affinity measurements between covalently bound mannose and ConA. All reactions were performed on a low molecular model level first and then transferred to telechelic and also side chain functionalized polymer systems.},
  subject      = {Polyoxazoline},
  language  = {en}
}
@article{PinznerKellerMutetal.2021,
  author    = {Pinzner, Florian and Keller, Thorsten and Mut, J{\"u}rgen and Bechold, Julian and Seibel, J{\"u}rgen and Groll, J{\"u}rgen},
  title     = {Polyoxazolines with a vicinally double-bioactivated terminus for biomacromolecular affinity assessment},
  series = {Sensors},
  volume    = {21},
  journal   = {Sensors},
  number    = {9},
  issn      = {1424-8220},
  doi       = {10.3390/s21093153},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-239530},
  year      = {2021},
  abstract  = {Interactions between proteins and carbohydrates with larger biomacromolecules, e.g., lectins, are usually examined using self-assembled monolayers on target gold surfaces as a simplified model measuring setup. However, most of those measuring setups are either limited to a single substrate or do not allow for control over ligand distance and spacing. Here, we develop a synthetic strategy, consisting of a cascade of a thioesterification, native chemical ligation (NCL) and thiol-ene reaction, in order to create three-component polymer conjugates with a defined double bioactivation at the chain end. The target architecture is the vicinal attachment of two biomolecule residues to the α telechelic end point of a polymer and a thioether group at the ω chain end for fixating the conjugate to a gold sensor chip surface. As proof-of-principle studies for affinity measurements, we demonstrate the interaction between covalently bound mannose and ConA in surface acoustic wave (SAW) and surface plasmon resonance (SPR) experiments.},
  language  = {en}
}