@article{KekowUlrichsMuellerRuchholtzetal.1988,
  author    = {Kekow, J{\"o}rn and Ulrichs, Karin and M{\"u}ller-Ruchholtz, Wolfgang and Gross, Wolfgang L.},
  title     = {Measurement of rat insulin. Enzyme-linked immunosorbent assay with increased sensitivity, high accuracy, and greater practicability than established radioimmunoassay},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-45543},
  year      = {1988},
  abstract  = {Total immunoreactive insulin (IRI) is conventionally determined by radioimmunoassays. IR! measurement in rats can be made more sensitive, accurate, and practical, as demonstrated by a new modified enzyme-linked immunosorbent assay (ELlSA). It is characterized by indirect binding of an anti-insulin antibody by an antiglobulin antibody and uses the principle of competitive saturation. In this ELlSA, IRI can be determined in a wide range of concentrations, corresponding to the standards. The standard curve ranges from 100 to 0.049 ng/mllRI (1 ng/ml - 23.4 JLU/ml - 172 pM rat insulin). The statistical analysis shows between- and within-assay coefficients of variation of :515\%. Diabetes 37:321-26,1988},
  subject      = {Chirurgie},
  language  = {en}
}