@article{SchartlSchluppSchartletal.1991, author = {Schartl, Manfred and Schlupp, Ingo and Schartl, Angelika and Meyer, Manfred K. and Nanda, Indrajit and Schmid, Michael and Epplen, J{\"o}rg T. and Parzefall, Jakob}, title = {On the stability of dispensable constituents of the eukaryotic genome: Stability of coding sequences versus truly hypervariable sequences in a clonal vertebrate, the amazon molly, Poecilia formosa}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-61731}, year = {1991}, abstract = {In dooal unisexual vertebrales, the genes specifying the males become dispensable. To study tbe rate of such geoes the gynogeoetic all-female fisb Poecilillfonnolll was treated with androgens. Phenotypic males were obtained that exbibited the complete set of male cbaracteristics of dosely related gooocboristic species, induding body proportions, pigmentation, the extremely complex insemination apparatus of poecil{\"u}d fish, sexual bebavior, and spermatogeoesls. Tbe apparent stabllity of such genic structures, induding those involved in androgen regulation, is contrasted by high instability of noncoding sequeaces. Frequent mutations, thelr donal transmission, and at least two truly hypervariable Iod leading to individual difl'ereaces between these othenrise donal organisms were detected by DNA fingerprinting. These observations substantiate the concept that also in "ameiotic" vertebrates certain compartments of the genome are more prooe to mutatiooal alterations than others.}, subject = {Physiologische Chemie}, language = {en} } @article{MeyerSchartl1979, author = {Meyer, Manfred K. and Schartl, Manfred}, title = {Eine neue Xiphophorus-Art aus Vera Cruz, Mexiko : (Pisces: Poeciliidae)}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-87124}, year = {1979}, abstract = {Xiphophorus andersi n. sp. from the Rio Atoyac, Vera Cruz, Mexico is described: lang head, moderately slender body, large dark black spar at the basis of the anal fin; adult male with short sword-like caudal appendage; rip of ray 5a of gonopodium without a developed claw. Xiphophorus andersi n. sp. differs by the combination of distinct characters from all the other species of the genus known so far. The new species shows features of both the so-called platyfish species group and the so-called swordtail species group.}, subject = {Schwertk{\"a}rpfling}, language = {en} } @article{MeyerSchartl1984, author = {Meyer, Manfred K. and Schartl, Manfred}, title = {Pseudotropheus (Maylandia) hajomaylandi n. sp., a new taxon from Lake Malawi}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-70989}, year = {1984}, abstract = {Pseudotropheus hajomaylandi (loc. typ. Isle of Chisumulu, Lake Malawi) is described as a new species. It is compared with Ps. aurora, Ps. greshakei, Ps. livingstonii, Ps. lombardoi, and Ps. zebra. All these taxa, including Ps. hajomaylandi and Ps. heteropictus, are classified in the subgenus Maylandia.}, subject = {Buntbarsche}, language = {en} } @incollection{EpplenAmmerEpplenetal.1991, author = {Epplen, J. T. and Ammer, H. and Epplen, C. and Kammerbauer, C. and Mitreiter, R. and Roewer, L. and Schwaiger, W. and Steimle, V. and Zischler, H. and Albert, E. and Andreas, A. and Beyermann, B. and Meyer, W. and Buitkamp, J. and Nanda, I. and N{\"u}rnberg, P. and Pena, S. D. J. and P{\"o}che, H. and Sprecher, W. and Schartl, Manfred and Weising, K. and Yassouridis, A.}, title = {Oligonucleotide fingerprinting using simple repeat motifs: a convenient, ubiquitously applicable method to detect hypervariability for multiple purposes}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-86371}, publisher = {Universit{\"a}t W{\"u}rzburg}, year = {1991}, abstract = {A panel of simple repetitive oligonucleotide probes has been designed and tested for multilocus DNA fingerprinting in some 200 fungal, plant and animal species as well as man. To date at least one of the probes has been found to be informative in each species. The human genome, however, has been the major target of many fingerprintins studies. Using the probe (CAC)5 or (GTG)5, individualization of all humans is possible except for monozygotic twins. Paternity analyses are now perfonned on a routine basis by the use of multilocus fingerprints, inctuding also cases of deficiency, i.e. where one of the parents is not available for analysis. In forensie science stain analysis is feasible in all tissue remains containing nuc)eated cells. Depending on the degree of DNA degradation a variety of oligonucleotides are informative, and they have been proven useful in actual case work. Advantages in comparison to other methods including enzymatic DNA amplification techniques (PCR) are evident. Fingerprint patterns of tumors may be changed due to the gain or loss of chromosomes and/or intrachromosomal deletion and amplification events. Locus-specific probes were isolated from the human (CAC)5/( GTG)5 fingerprint with a varying degree of informativeness (monomorphic versus truly hypervariable markers). The feasibility of three different approaches. for the isolation of hypervariable mono-locus probes was evaluated. Finally, one particular mixed simple (gt)n(ga)m repeat locus in the second intron of the HLA-DRB genes has been scrutinized to allow comparison of the extent of exon-encoded (protein-) polymorphisms versus intronie bypervariability of simple repeats: adjacent to a single gene sequence (e.g. HLA-DRB1*0401) many different length alleles were found. Group-specific structures of basic repeats were identified within the evolutionarily related DRB alleles. As a further application it is suggested here that due to the ubiquitous interspersion of their targets, short probes for simple repeat sequences are especially useful tools for ordering genomic cosmid, yeast artificial chromosome and phage banks.}, subject = {DNS}, language = {en} }