@article{HazurDetschKarakayaetal.2020,
  author    = {Hazur, Jonas and Detsch, Rainer and Karakaya, Emine and Kaschta, Joachim and Teßmar, J{\"o}rg and Schneidereit, Dominik and Friedrich, Oliver and Schubert, Dirk W and Boccaccini, Aldo R},
  title     = {Improving alginate printability for biofabrication: establishment of a universal and homogeneous pre-crosslinking technique},
  series = {Biofabrication},
  volume    = {12},
  journal   = {Biofabrication},
  number    = {4},
  doi       = {10.1088/1758-5090/ab98e5},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-254030},
  year      = {2020},
  abstract  = {Many different biofabrication approaches as well as a variety of bioinks have been developed by researchers working in the field of tissue engineering. A main challenge for bioinks often remains the difficulty to achieve shape fidelity after printing. In order to overcome this issue, a homogeneous pre-crosslinking technique, which is universally applicable to all alginate-based materials, was developed. In this study, the Young's Modulus after post-crosslinking of selected hydrogels, as well as the chemical characterization of alginate in terms of M/G ratio and molecular weight, were determined. With our technique it was possible to markedly enhance the printability of a 2\% (w/v) alginate solution, without using a higher polymer content, fillers or support structures. 3D porous scaffolds with a height of around 5 mm were printed. Furthermore, the rheological behavior of different pre-crosslinking degrees was studied. Shear forces on cells as well as the flow profile of the bioink inside the printing nozzle during the process were estimated. A high cell viability of printed NIH/3T3 cells embedded in the novel bioink of more than 85\% over a time period of two weeks could be observed.},
  language  = {en}
}