@article{ZvirblisGorboulevRubtsovetal.1988,
  author    = {Zvirblis, G. S. and Gorboulev, Valentin G. and Rubtsov, P. M. and Chernov, B. K. and Golova, Yu. B. and Pozmogova, G. E. and Skryabin, K. G. and Bayev, A. A.},
  title     = {Genetic engineering of peptide hormones : III. Cloning of cDNA of porcine growth hormone and construction of gene for expression of hormone in bacteria},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-46958},
  year      = {1988},
  abstract  = {Results are presented of cloning cDNA of procine growth hormone, analysis of its primary structure, and creation of a construction capable of expression of this cDNA in Esqheriahia coti cells. It is shown that in the population of mRNA coding porcine growth hormone, heterogeneity is noted which is manifested not only at the level of the nucleotide sequence, but also is reflected in the amino acid sequence of the mature hormone.},
  language  = {en}
}
@article{RubtsovChernovGorboulevetal.1985,
  author    = {Rubtsov, P. M. and Chernov, V. G. and Gorboulev, Valentin G. and Parsadanyan, A. S. and Sverdlova, P. S. and Chupeeva, V. V. and Golova, Yu. B. and Batchikova, N. V. and Zvirblis, G. S. and Skryabin, K. G. and Bayev, A. A.},
  title     = {Genetic engineering of peptide hormones},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-46964},
  year      = {1985},
  abstract  = {Peptide and polypeptide hormones represent an extensive group of biologically active compounds of important significance for medicine and agriculture. In recent years genetic engineering methods have been used to create strains of microorganisms synthesizing eukaryotic proteins, including hormones and their precursors. The first stage of such developments is the isolation of DNA coding the des~red product. We have accomplished the cloning of the cDNA of a number of polypeptide and peptide hormones of the pituitary of man and domestic animals. The model gene of human calcitonin has also been synthesized and cloned. The obtained genes are being used to develop methods for the microbiological synthesis of human and animal-hormones.},
  language  = {en}
}
@article{RubtsovOganessyanGorboulevetal.1988,
  author    = {Rubtsov, P. M. and Oganessyan, R. G. and Gorboulev, Valentin G. and Skryabin, K. G. and Bayev, A. A.},
  title     = {Genetic engineering of peptide hormones : II. Possible polymorphism of preprolactin in cattle. Data of molecular cloning},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-46975},
  year      = {1988},
  abstract  = {Primary structure is determined of an insertion of a clone isolated from the library of hypophyseal cDNA of cattle by hybridization with a probe specific for prolactin. Analysis of nucleotide sequences showed that in the process of cloning, reorganization occurred in structure of preprolactin cDNA, including an inversion of the 5'-terminal and deletion of the central section of cDNA. Nevertheless, from structure of cDNA, nucleotide sequences can be deduced of extended 5'- and 3'-terminal sections of preprolactin mRNA in cattle with lengths of 257 and 551 nucleotide residues, respectively. When these sequences are compared to those established previously, some differences were found in primary structure. The most important of them is the presence of an additional codon which codes alanine at the position (-22) of the signal peptide. It is suggested that heterogeneity of preprolactin mRNA of cattle in the section coding the signal peptide is the result of alternative splicing, as was shown for preprolactin mRNA in rats.},
  language  = {en}
}
@article{ArtyukovaGorboulevRodionovaetal.1985,
  author    = {Artyukova, E. V. and Gorboulev, Valentin G. and Rodionova, N. P. and Krylov, A. V. and Atabekov, J. G.},
  title     = {Comparative study of structural peculiarities and translation of potexvirus RNAs},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-46985},
  year      = {1985},
  abstract  = {Structural peculiarities of the S'-end segments of genomic RNA were studied in F potato virus (F-PV) and white clover mosaic virus (WCMV). The methods of affinity chromatography on oligo(dT) cellulose and oligonucleotide mapping revealed a prolonged (up to 210 nucleotides) polyadenyl sequence at the 3'-end of F-PV RNA. A polyadenyl sequence is missing at the 3'end of WCMV RNA. A study of the translation products of WCMV and F-PV RNAs in a oe11-free protein-synthesizing system derived from rabbit reticulocytes showed that polypeptides electrophoretically comigrating with a structural protein of either virus were synthesized alongside high-molecular-weight polypeptides (M\(_r\)\(\approx\) 180-150 kdaltons).},
  language  = {en}
}
@article{BoriskinDesyatskovaBogomolovaetal.1986,
  author    = {Boriskin, Yu S. and Desyatskova, R. G. and Bogomolova, N. N. and Gorboulev, Valentin G.},
  title     = {Stability of rubella virus after long-term persistence in human cell line},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-46944},
  year      = {1986},
  abstract  = {Primary infection of HEp-2 cells with rubella virus resulted in non-cytophatic longterm persistent infection. During four years of persistence the virus was produced in sufficient quantities (up to 6 logs PFU/ml) and did not differ from the parental variant in its pathogenicity for BHK-21 or RK-13 cells, or hemagglutinating activity, but formed smaller plaques. Persistent virus preserved the original antigenicity as judged from reciprocal hemagglutination-inhibition or plaque reduction-neutralization tests with polyclonal antisera. Both original and persistent rubella viruses were thermoresistant (T 56° C) and sligthly temperature-sensitive. Clonal analysis revealed presence of ts-mutants among both original and persistent virus clones with different degrees of plating efficiency at 40°/34° C. RNA fingerprinting showed only minor changes in persistent rubella virus.},
  language  = {en}
}
@article{TsfasmanNesmeyanovaGorboulevetal.1989,
  author    = {Tsfasman, I. M. and Nesmeyanova, M. A. and Gorboulev, Valentin G. and Rubtsov, P. M. and Skryabin, K. G.},
  title     = {Biosynthesis and secretion of bovine growth hormone in Escherichia coli under the control of the secretory vector containing a promoter and signal sequence of alkaline phosphatase gene},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-46932},
  year      = {1989},
  abstract  = {A recombinant plasmid was constructed containing the gene for bovine growth hormone joinea with the regulatory region and the region coding the signal sequence of the Escherichia coli alkaline phosphatase gene. In conditions of phosphorus starvation, which c~s derepression of alkaline phosphatase, expression was shown of the gene for bovine growth hormone, in addition to partial processing and secretion of protein into periplasm.},
  language  = {en}
}
@article{AgranovskyDolyaGorboulevetal.1981,
  author    = {Agranovsky, A. A. and Dolya, V. V. and Gorboulev, Valentin G. and Kozlov, J. V. and Atabekov, J. G.},
  title     = {Aminoacylation of barley stripe mosaic virus RNA: polyadenylate-containing RNA has a 3'-terminal tyrosine-accepting structure},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32566},
  year      = {1981},
  abstract  = {Barley stripe mosaic virus (BSMV) RNA which was previously reported to contain poly(A) sequences (Agranovsky et al., 1978) can be specifically esterified with tyrosine in vitro in the presence of an aminoacyl-tRNA synthetase fraction from wheat embryos. All the three RNA components of the BSMV strain with a three-component genome (Norwich) and both RNA components of a two-component strain (Russian) can be tyrosylated. The poly(A)-containing (bound to oligo(dT)-cellulose) and poly(A)-deficient(not bound to oligo(dT)-cellulose) fractions of BSMV RNA display a similar amino acidaccepting ability. The nucleotide sequence which accepts tyrosine is coupled with the intact genomic polyadenylated BSMV RNA. The viral RNA isolated after sucrose density gradient centrifugation under drastic denaturing conditions retains its aminoacylating activity, which suggests that this activity is not due to the presence in a BSMV RNA preparation of a tyrosine tRNA associated with BSMV RNA. Inhibition of aminoacylation of the 3'-oxidized (treated with sodium metaperiodate) BSMV RNA suggests that the tyrosine-accepting structure is localized at the 3' terminus of BSMV RNA molecules. It is shown that segments of different lengths obtained upon random fragmentation can be tyrosylated. The 3'-terminal (tyrosine-accepting) poly(A)+ segments can be isolated. The shortest segments of viral RNA capable of being aminoacylated [i.e., containing both tRNA-like structure and poly(A)] consists of approximately 150-200 nucleotides. The analysis of the oligonucleotides derived from individual BSMV RNA components labeled with 32P at the 3' end revealed two types of 3'-terminal sequences different from poly(A). It is suggested that a poly(A) sequence is intercalated between a 3'-terminal tyrosineaccepting structure and the 5'-terminal portion of poly(A)+ BSMV RNA.},
  language  = {en}
}
@article{KozlovGorboulevKurmanovaetal.1981,
  author    = {Kozlov, J. V. and Gorboulev, Valentin G. and Kurmanova, A. G. and Bayev, Alexander A. and Shilov, A. A. and Zhdanov, V. M.},
  title     = {On the origin of the H1N1 (A/USSR/90/77) influenza virus},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32556},
  year      = {1981},
  abstract  = {The influenza virus H1N1 (the A/USSR/90/77 strain) that reappeared in 1977 after the H1N1 influenza viruses had disappeared from the human population, is compared with the A/FM/1/47 and the A/FW/1/50 influenza viruses by the method of oligonucleotide mapping of individual segments of the viral RNAs. Seven genes of the A/USSR/90/77 virus appear to be very similar to the corresponding genes of the A/FW/1/50 virus, whereas the gene coding for the M protein displays considerable homology to the corresponding gene of the A/FM/1/47 virus. The data demonstrate that the A/USSR/90/77 strain is a recombinant virus.},
  language  = {en}
}
@article{KutateladzeAxelrodGorboulevetal.1979,
  author    = {Kutateladze, T. V. and Axelrod, V. D. and Gorboulev, Valentin G. and Belzhelarskaya, S. N. and Vartikyan, R. V.},
  title     = {New procedure of high-voltage electrophoresis in polyacrylamide gel and its application to the sequencing of nucleic acids},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-46927},
  year      = {1979},
  abstract  = {Fractionation of nucleic acids and their fragments with polyacrylamide gel has been widely applied in sequencing of nucleic acids. Although the conditions of electrophoresis for this purpose have previously been suggested. we have found that polyacrylamide gel electrophoresis at 5000 V (100 V/cm) is possible and effective. An apparatus consisting of a horizontal thermostated plate is used to remove the heat which was formed during the electrophoretic process. The techniques for loading samples on the horizontal thin gel and the procedure for high-voltage gel electrophoresis are described and illustrated by the fractionation of the spleen phosphodiesterase partial digest of tRNA¥~1 as well as by the RNA synthesis by RNA polymerase from E. coli with poly[d(A- T)j as template in the presence of "terminator," 3'-O-methyluridine 5'-triphosphate. This same technique was used for electrophoresis of oligonucleotides on acetylcellulose and was incorporated into a two-dimensional system which was demonstrated by fingerprinting of the guanylo-RNase digest of tRNAT'P from baker's yeast. In the third part of the article a simple technique for the electric trapping of nucleic acids or their fragments from a slab gel on a DEAE-paper sheet is presented.},
  language  = {en}
}
@inproceedings{AxelrodGorboulevKutateladzeetal.1976,
  author    = {Axelrod, V. D. and Gorboulev, Valentin G. and Kutateladze, T. V. and Barciszewski, J. and Bayev, A. A.},
  title     = {The new approach to tRNA primary structure determination : the primary structure of valine tRNA\(^{Val}_{2b}\)},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-50920},
  year      = {1976},
  abstract  = {The new combination of TLC and high voltage electrophoresis on cooling plate is described.We have applied this technique to study of primary structure of tRNA.Preliminary sequence of baker's yeast tRNA^Val_2b is described. New approach to preparation of large tRNA fragments is demonstrated.},
  subject      = {RNS},
  language  = {en}
}
@article{GorboulevAkhundovaGrzeschiketal.1994,
  author    = {Gorboulev, Valentin G. and Akhundova, Aida and Grzeschik, K.-H. and Fahrenholz, Falk},
  title     = {Organization and chromosomal localization of the gene for the human bombesin receptor subtype expressed in pregnant uterus},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32572},
  year      = {1994},
  abstract  = {The gene encoding the human homologue of the guinea pig uterine bombesm receptor [( 1992) Eur. J. Biochem. 208,405] was isolated from a genomic lambda library by the PCR/homology screening approach. The gene spans more than 4 kb and consists of 3 exons and 2 introns. The deduced amino acid sequence shows about 86\% identity to that of guinea pig bombesin receptor. This subtype of bombesin receptor is expressed in the pregnant uterus and in two human tumour cell lines, T47D (ductal breast carcinoma) and A431 (epidermal carcinoma). PCR analysis of genomic DNA from human-mouse cell hybrids allows the cloned gene to be localized to the region q26q28 on chromosome X.},
  language  = {en}
}
@article{GorboulevKutateladzeBarciszewskietal.1977,
  author    = {Gorboulev, Valentin G. and Kutateladze, Tamara V. and Barciszewski, Jan and Axelrod, Vladimir D.},
  title     = {The separation of oligonucleotides of baker's yeast valine transfer ribonucleic acid 2b by high-voltage electrophoresis on DEAE-paper and by thin-layer chromatography},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32536},
  year      = {1977},
  abstract  = {No abstract available},
  language  = {en}
}
@article{GorboulevAxelrodBayev1977,
  author    = {Gorboulev, Valentin G. and Axelrod, Vladimir D. and Bayev, Alexander A.},
  title     = {Primary structure of baker's yeast valine tRNA\(^{Val}_{2b}\)},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32546},
  year      = {1977},
  abstract  = {The minor form of vallne tBNA from baker's yeaat - tRNA\(^{Val}_{2b}\) - purified by column chromatography was completely digesteft with guanylo-BNase and pancreatic ENase. The products of these digestions were separated by a combination of thin-layer chromatography on cellulose and high voltage electrophoresis on DEAE-paper and then identified. The halves of tRNA Val 2b were prepared by partial digestion with pancreatic Mass and their complete guanylo-BNase and pancreatic ENase, digests were analysed. Basing on the obtained data the primary structure of baker1s yeast tRNA\(^{Val}_{2b}\) was reconstructed.},
  language  = {en}
}