@article{HeiserUlrichsMuellerBuchholtz1994,
  author    = {Heiser, Axel and Ulrichs, Karin and M{\"u}ller-Buchholtz, Wolfgang},
  title     = {Isolation of porcine pancreatic islets: low trypsin activity during the isolation procedure guarantees reproducible high islet yields},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-44719},
  year      = {1994},
  abstract  = {During the past few years, interest in xenotransplantation of porcine islets of Langerhans for the future therapy of type I diabetes has Increased markedly. Therefore, we established a semiautomated digestion method for isolating islets from the porcine pancreas. However, although the isolation technique was standardized and collagenase of controlled quality was used, we were unable to attain high islet yields with a satisfactory degree of reproducibility. One hypothesis was that varying degrees of interference by donor pancreatic enzymes were responsible for this failure. The aim of this stUdy was to examine the kinetics of four types of enzymatic activity during the isolation procedure, as well as their effects on islet yield: collagenase, trypsin, neutral protease, and clostripaln. Our results indicate that while exogenous collagenase activity decreases slightly during the isolation procedure, the activity of the pancreas enzymes neutral protease and trypsin increases. In some cases, trypsin activity increases very strongly. A strong increase in trypsin activity correlates with poor islet yield, whereas low trypsin activity always correlates with high islet yield. Addition of the protease inhibitor Pefabloc to the isolation medium results in low trypsin activity and reproducible high islet yields.},
  subject      = {Langerhans-Inseln},
  language  = {en}
}
@article{WaagaKrzymanskiUlrichsetal.1993,
  author    = {Waaga, AM and Krzymanski, M. and Ulrichs, Karin and Wierusz-Wysocka, Bogna and M{\"u}ller-Buchholtz, Wolfgang},
  title     = {Hematological effects of the new immunosuppressive drug 15-deoxyspergualin},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-44701},
  year      = {1993},
  abstract  = {Since systematic hematological studies on blood and bone marrow changes after treatment with 15-Deoxyspergualin (DOS) are lacking, a quantitative assessment was performed fourteen or twenty eight days after intraperitoneal application of DOS to rats. Further observations done 7 and 14 days after discontinuation of DOS administration allowed analysis of banc marrow regeneration. DOS induced lymphocytopenia, granUlocytopenia and anemia with a decrease of bone marrow cellularity due to suppression of cell maturation. The effect was dose-dependent and bone marrow as well as blood changes were observed in animals treated with doses from 0.5 to 10.0 mg/kg DOS. Within 14 days after termination of the treatment, rapid recovery with normalization of all hematological parameters was observed. In the light of our data, these hematological side effects may not be a major disadvantage, if DOS is used in doses below 2.5 mg/kg, and for a course of therapy which is limited to 7 to 14 days.},
  subject      = {Chirurgie},
  language  = {en}
}