@article{PereiraTrivanovićStahlhutetal.2022,
  author    = {Pereira, Ana Rita and Trivanović, Drenka and Stahlhut, Philipp and Rudert, Maximilian and Groll, J{\"u}rgen and Herrmann, Marietta},
  title     = {Preservation of the na{\"i}ve features of mesenchymal stromal cells in vitro: Comparison of cell- and bone-derived decellularized extracellular matrix},
  series = {Journal of Tissue Engineering},
  volume    = {13},
  journal   = {Journal of Tissue Engineering},
  doi       = {10.1177/20417314221074453},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-268835},
  pages     = {1-12},
  year      = {2022},
  abstract  = {The fate and behavior of bone marrow mesenchymal stem/stromal cells (BM-MSC) is bidirectionally influenced by their microenvironment, the stem cell niche, where a magnitude of biochemical and physical cues communicate in an extremely orchestrated way. It is known that simplified 2D in vitro systems for BM-MSC culture do not represent their na{\"i}ve physiological environment. Here, we developed four different 2D cell-based decellularized matrices (dECM) and a 3D decellularized human trabecular-bone scaffold (dBone) to evaluate BM-MSC behavior. The obtained cell-derived matrices provided a reliable tool for cell shape-based analyses of typical features associated with osteogenic differentiation at high-throughput level. On the other hand, exploratory proteomics analysis identified native bone-specific proteins selectively expressed in dBone but not in dECM models. Together with its architectural complexity, the physico-chemical properties of dBone triggered the upregulation of stemness associated genes and niche-related protein expression, proving in vitro conservation of the na{\"i}ve features of BM-MSC.},
  language  = {en}
}
@article{vonHertzbergBoelchLuedemannRudertetal.2022,
  author    = {von Hertzberg-Boelch, Sebastian Philipp and Luedemann, Martin and Rudert, Maximilian and Steinert, Andre F.},
  title     = {PMMA bone cement: antibiotic elution and mechanical properties in the context of clinical use},
  series = {Biomedicines},
  volume    = {10},
  journal   = {Biomedicines},
  number    = {8},
  issn      = {2227-9059},
  doi       = {10.3390/biomedicines10081830},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-281708},
  year      = {2022},
  abstract  = {This literature review discusses the use of antibiotic loaded polymethylmethacrylate bone cements in arthroplasty. The clinically relevant differences that have to be considered when antibiotic loaded bone cements (ALBC) are used either for long-term implant fixation or as spacers for the treatment of periprosthetic joint infections are outlined. In this context, in vitro findings for antibiotic elution and material properties are summarized and transferred to clinical use.},
  language  = {en}
}
@article{StratosRinasSchroepferetal.2023,
  author    = {Stratos, Ioannis and Rinas, Ingmar and Schr{\"o}pfer, Konrad and Hink, Katharina and Herlyn, Philipp and B{\"a}umler, Mario and Histing, Tina and Bruhn, Sven and M{\"u}ller-Hilke, Brigitte and Menger, Michael D. and Vollmar, Brigitte and Mittlmeier, Thomas},
  title     = {Effects on bone and muscle upon treadmill interval training in hypogonadal male rats},
  series = {Biomedicines},
  volume    = {11},
  journal   = {Biomedicines},
  number    = {5},
  issn      = {2227-9059},
  doi       = {10.3390/biomedicines11051370},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-319266},
  year      = {2023},
  abstract  = {Testosterone deficiency in males is linked to various pathological conditions, including muscle and bone loss. This study evaluated the potential of different training modalities to counteract these losses in hypogonadal male rats. A total of 54 male Wistar rats underwent either castration (ORX, n = 18) or sham castration (n = 18), with 18 castrated rats engaging in uphill, level, or downhill interval treadmill training. Analyses were conducted at 4, 8, and 12 weeks postsurgery. Muscle force of the soleus muscle, muscle tissue samples, and bone characteristics were analyzed. No significant differences were observed in cortical bone characteristics. Castrated rats experienced decreased trabecular bone mineral density compared to sham-operated rats. However, 12 weeks of training increased trabecular bone mineral density, with no significant differences among groups. Muscle force measurements revealed decreased tetanic force in castrated rats at week 12, while uphill and downhill interval training restored force to sham group levels and led to muscle hypertrophy compared to ORX animals. Linear regression analyses showed a positive correlation between bone biomechanical characteristics and muscle force. The findings suggest that running exercise can prevent bone loss in osteoporosis, with similar bone restoration effects observed across different training modalities.},
  language  = {en}
}
@phdthesis{Seiler2023,
  author    = {Seiler, Jonas},
  title     = {Die Expression des Vitamin-D-Rezeptors und der 24-Hydroxylase in Knochenmetastasen unterschiedlicher Entit{\"a}t},
  doi       = {10.25972/OPUS-32182},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-321827},
  school      = {Universit{\"a}t W{\"u}rzburg},
  year      = {2023},
  abstract  = {Knochenmetastasen sind unter den drei h{\"a}ufigsten Manifestationsorten metastatischer Absiedelungen von fortgeschrittenen Tumorerkrankungen. Dabei sind insbesondere Patientinnen und Patienten mit Prostata- und Mammakarzinom von Knochenmetastasen betroffen. Diese Knochenmetastasen f{\"u}hren h{\"a}ufig zu einer deutlichen Verschlechterung der Lebensqualit{\"a}t und zu einer Begrenzung der Therapieoptionen auf lediglich palliative Ans{\"a}tze. Die biologisch aktive Form von Vitamin D3, 1,25(OH)2-Vitamin D3, zeigt in pr{\"a}klinischen Studien antiproliferative und differenzierende Effekte auf Tumorzellen (101, 102, 104), die haups{\"a}chlich durch die Bindung an den Vitamin D-Rezeptor (VDR) vermittelt werden. Dar{\"u}berhinaus konnte pr{\"a}klinisch gezeigt werden, dass eine niedrige Expression des VDRs, ligandenunabh{\"a}ngig, die Knochenmetastasierung und das Tumorwachstum beg{\"u}nstigt (118). Eine niedrige VDR-Expression ist in Prim{\"a}rtumoren in klinischen Studien mit aggressiven Tumoreigenschaften assoziiert (111, 113, 115) und kann zudem mit einer erh{\"o}hten/fr{\"u}heren oss{\"a}ren Metastasierung einhergehen (167). Zudem gibt es Hinweise auf einen dysregulierten 1,25(OH)2-Vitamin D3-Katabolismus durch eine erh{\"o}hte Expression des 1,25(OH)2-Vitamin D3 katabolisierenden Enzyms CYP24A1/24-Hydroxylase in prim{\"a}rem Tumorzellen (70, 121, 122). Durch die Untersuchungen der Prim{\"a}rtumoren ist damit zu hypothetisieren, dass die Expression des VDRs und von CYP24A1 bei der Tumorprogression und Knochenmetastasierung von Bedeutung sein k{\"o}nnte. Entsprechende Untersuchungen des VDRs und der 24-Hydroxylase in Knochenmetastasen fehlen allerdings. Deshalb wurde in dieser Arbeit die Expression des VDRs und von CYP24A1 in Knochenmetastasen unterschiedlicher Prim{\"a}rtumoren von 66 Patientinnen und Patienten untersucht und m{\"o}gliche Assoziationen mit aggressiven Tumoreigenschaften analysiert. Der VDR konnte sowohl im Zytoplasma als auch im Nukleus nachgewiesen werden, w{\"a}hrend CYP24A1 nur im Zytoplasma lokalisiert war. Dabei wiesen insgesamt 71 \% der Knochenmetastasen eine hohe VDR-Expression im Nukleus und 56 \% im Zytoplasma auf. 59 \% der Knochenmetastasen wiesen eine hohe Expression des VDRs insgesamt auf. CYP24A1 war ebenso in 59 \% der Knochenmetastasen hoch exprimiert. Bei der Auswertung des Zusammenhangs zwischen den TNM-Stadien und des Gradings zeigte sich ein nicht signifikanter Trend von schlecht differenzierten Tumoren hin zu einer niedrigeren nukle{\"a}ren VDR-Expression (p=0.07, siehe Abbildung 33). Bez{\"u}glich der T-Stadien zeigten sich keine Unterschiede der Expression des VDRs und von CYP24A1 in den Knochenmetastasen zwischen lokal fortgeschrittenen und kleinen Prim{\"a}rtumoren. Weiterhin hatten Patientinnen und Patienten mit Lymphknotenmetastasen tendenziell eine verminderte VDR- und auch CYP24A1-Expression in den Knochenmetastasen im Vergleich zu Patienten und Patientinnen ohne Lymphknotenmetastasen (pVDR=0.15, pCYP24A1=0.06, siehe Abbildung 35). Außerdem hatten Patientinnen und Patienten mit multiple metastasierten Tumoren eine signifikant niedrigere nukle{\"a}re VDR- und auch CYP24A1-Expression im Vergleich zu Patientinnen und Patienten mit ausschließlich oss{\"a}rer Metastasierung (pVDR=0.03, pCYP24A1=0.01, Abbildung 36). Die Proteinexpression des VDRs- und von CYP24A1 korrelierten signifikant (p=0.001). Somit konnte mit dieser Arbeit die Proteinexpression des VDRs und von CYP24A1 in Knochenmetastasen durch Immunhistologie nachgewiesen werden. Insgesamt wurde der VDR und CYP24A1 von Knochenmetastasen diverser Entit{\"a}t unterschiedlich stark exprimiert. Jedoch k{\"o}nnten insbesondere Patienten mit VDR-exprimierenden Knochenmetastasen von einer Vitamin D3-Supplementierung profitieren, die h{\"a}ufig einen 25-OH-Vitamin D3 Mangel zeigen (165, 166). Ebenso k{\"o}nnte eine Untersuchung auf einen niedrigen VDR-Status in Prim{\"a}rtumoren dabei helfen, Krebspatienten mit einem hohen Metastasierungsrisiko zu identifizieren. Allerdings sind weitere und gr{\"o}ßere Studien inbesondere mit Evaluation des gesamten Vitamin D-Metabolismus und -Signalwegs notwendig, um diesen Zusammenhang weiter zu untersuchen.},
  subject      = {Vitamin D3},
  language  = {de}
}
@article{WangStoecklLietal.2022,
  author    = {Wang, Chenglong and St{\"o}ckl, Sabine and Li, Shushan and Herrmann, Marietta and Lukas, Christoph and Reinders, Yvonne and Sickmann, Albert and Gr{\"a}ssel, Susanne},
  title     = {Effects of extracellular vesicles from osteogenic differentiated human BMSCs on osteogenic and adipogenic differentiation capacity of na{\"i}ve human BMSCs},
  series = {Cells},
  volume    = {11},
  journal   = {Cells},
  number    = {16},
  issn      = {2073-4409},
  doi       = {10.3390/cells11162491},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-286112},
  year      = {2022},
  abstract  = {Osteoporosis, or steroid-induced osteonecrosis of the hip, is accompanied by increased bone marrow adipogenesis. Such a disorder of adipogenic/osteogenic differentiation, affecting bone-marrow-derived mesenchymal stem cells (BMSCs), contributes to bone loss during aging. Here, we investigated the effects of extracellular vesicles (EVs) isolated from human (h)BMSCs during different stages of osteogenic differentiation on the osteogenic and adipogenic differentiation capacity of na{\"i}ve (undifferentiated) hBMSCs. We observed that all EV groups increased viability and proliferation capacity and suppressed the apoptosis of na{\"i}ve hBMSCs. In particular, EVs derived from hBMSCs at late-stage osteogenic differentiation promoted the osteogenic potential of na{\"i}ve hBMSCs more effectively than EVs derived from na{\"i}ve hBMSCs (na{\"i}ve EVs), as indicated by the increased gene expression of COL1A1 and OPN. In contrast, the adipogenic differentiation capacity of na{\"i}ve hBMSCs was inhibited by treatment with EVs from osteogenic differentiated hBMSCs. Proteomic analysis revealed that osteogenic EVs and na{\"i}ve EVs contained distinct protein profiles, with pro-osteogenic and anti-adipogenic proteins encapsulated in osteogenic EVs. We speculate that osteogenic EVs could serve as an intercellular communication system between bone- and bone-marrow adipose tissue, for transporting osteogenic factors and thus favoring pro-osteogenic processes. Our data may support the theory of an endocrine circuit with the skeleton functioning as a ductless gland.},
  language  = {en}
}
@article{NedopilHowellHull2022,
  author    = {Nedopil, Alexander J. and Howell, Stephen M. and Hull, Maury L.},
  title     = {A TKA insert with a lateral flat articular surface maximizes external and internal tibial orientations without anterior lift-off relative to low- and ultracongruent surfaces},
  series = {Journal of Personalized Medicine},
  volume    = {12},
  journal   = {Journal of Personalized Medicine},
  number    = {8},
  issn      = {2075-4426},
  doi       = {10.3390/jpm12081274},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-286142},
  year      = {2022},
  abstract  = {Background: In total knee arthroplasty (TKA), inserts can have different levels of medial and lateral congruency determined by the acuteness of the upslopes of the anterior and posterior articular surfaces. The present study evaluated an insert with different levels of lateral congruency and a medial ball-in-socket congruency to test the hypothesis that a lateral flat (F) insert maximizes external tibial orientation at extension and internal orientation at 90° flexion and lowers the incidence of anterior lift-off relative to low-congruent (LC) and ultracongruent (UC) lateral inserts. Methods: Two surgeons treated 23 patients with unrestricted caliper-verified kinematic alignment (KA) and posterior cruciate ligament (PCL) retention. They randomly trialed inserts with a medial radial dial that functioned as a built-in goniometer by measuring the tibial orientation relative to a sagittal line on the femoral trial component. Anterior lift-off of the insert from the baseplate indicated PCL tightness. Results: The F insert's mean of 9° of external tibial orientation was higher than that of the LC (5°, p < 0.0001) and UC inserts (2°, p < 0.0001). The -13° of internal tibial orientation at 90° flexion was higher than that of the LC (-9°, p < 0.0001) and UC inserts (-7°, p < 0.0001). The 0\% incidence of anterior lift-off was less than that of the LC (26\%) and UC inserts (57\%) (p < 0.0001). Conclusions: Surgeons and implant manufacturers should know that adding congruency to the lateral articular surface limits external tibial orientation in extension and internal tibial orientation at 90° flexion and overtightens the PCL. These rotational limitations and flexion space tightness can adversely affect patellofemoral tracking and knee flexion.},
  language  = {en}
}
@article{NedopilHowellHull2022,
  author    = {Nedopil, Alexander J. and Howell, Stephen M. and Hull, Maury L.},
  title     = {Measurement of tibial orientation helps select the optimal insert thickness to personalize PCL tension in a medial ball-in-socket TKA},
  series = {Journal of Personalized Medicine},
  volume    = {12},
  journal   = {Journal of Personalized Medicine},
  number    = {9},
  issn      = {2075-4426},
  doi       = {10.3390/jpm12091427},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-286232},
  year      = {2022},
  abstract  = {As the conformity of a medial ball-in-socket total knee arthroplasty (TKA) provides intrinsic anterior-posterior (A-P) stability, surgeons cannot rely on the manual examination of sagittal laxity to identify the optimal insert thickness. Instead, the present study determined whether measuring tibial axial orientation in extension and 90° flexion with an insert goniometer could identify the optimal thickness that, when implanted, provides high postoperative function. In twenty-two patients that underwent unrestricted caliper-verified kinematic alignment (KA) with a PCL retaining implant, two surgeons measured tibial orientation in extension and 90° flexion with 10, 11, 12, and 13 mm thick insert goniometers. Each TKA had one insert thickness that restored either the maximum external tibial orientation in extension, the maximum internal tibial orientation at 90° flexion, or both relative to 1 mm thinner and thicker inserts. In addition, the 6-month median [interquartile range] Forgotten Joint Score of 73 (54-87) and Oxford Knee Score of 42 (38-45) indicated high satisfaction and function. In conclusion, surgeons using a medial ball-in-socket TKA design can measure external tibial orientation in extension and internal tibial orientation at 90° flexion with an insert goniometer. Furthermore, implanting an insert with the thickness that provided the maximum orientation values resulted in high postoperative function, thereby personalizing PCL tension.},
  language  = {en}
}
@article{DhaliwalZamoraNedopiletal.2022,
  author    = {Dhaliwal, Anand and Zamora, Tomas and Nedopil, Alexander J. and Howell, Stephen M. and Hull, Maury L.},
  title     = {Six commonly used postoperative radiographic alignment parameters do not predict clinical outcome scores after unrestricted caliper-verified kinematically aligned TKA},
  series = {Journal of Personalized Medicine},
  volume    = {12},
  journal   = {Journal of Personalized Medicine},
  number    = {9},
  issn      = {2075-4426},
  doi       = {10.3390/jpm12091468},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-288186},
  year      = {2022},
  abstract  = {Background: Unrestricted caliper-verified kinematically aligned (KA) TKA restores patient's prearthritic coronal and sagittal alignments, which have a wide range containing outliers that concern the surgeon practicing mechanical alignment (MA). Therefore, knowing which radiographic parameters are associated with dissatisfaction could help a surgeon decide whether to rely on them as criteria for revising an unhappy patient with a primary KA TKA using MA principles. Hence, we determined whether the femoral mechanical angle (FMA), hip-knee-ankle angle (HKAA), tibial mechanical angle (TMA), tibial slope angle (TSA), and the indicators of patellofemoral tracking, including patella tilt angle (PTA) and the lateral undercoverage of the trochlear resection (LUCTR), are associated with clinical outcome scores. Methods: Forty-three patients with a CT scan and skyline radiograph after a KA TKA with PCL retention and medial stabilized design were analyzed. Linear regression determined the strength of the association between the FMA, HKA angle, PTS, PTA, and LUCTR and the forgotten joint score (FJS), Oxford knee score (OKS), and KOOS Jr score obtained at a mean of 23 months. Results: There was no correlation between the FMA (range 2° varus to -10° valgus), HKAA (range 10° varus to -9° valgus), TMA (range 10° varus to -0° valgus), TSA (range 14° posterior to -4° anterior), PTA (range, -10° medial to 14° lateral), and the LUCTR resection (range 2 to 9 mm) and the FJS (median 83), the OKS (median 44), and the KOOS Jr (median 85) (r = 0.000 to 0.079). Conclusions: Surgeons should be cautious about using postoperative FMA, HKAA, TMA, TSA, PTA, and LUCTR values within the present study's reported ranges to explain success and dissatisfaction after KA TKA.},
  language  = {en}
}
@article{HeinzWildEidmannetal.2023,
  author    = {Heinz, Tizian and Wild, Moritz and Eidmann, Annette and Weißenberger, Manuel and Rak, Dominik and Nedopil, Alexander Johannes and Rudert, Maximilian and Stratos, Ioannis},
  title     = {Impact of COVID-19 on fracture incidence in germany: a comparative age and gender analysis of pre- and post-outbreak periods},
  series = {Healthcare},
  volume    = {11},
  journal   = {Healthcare},
  number    = {15},
  issn      = {2227-9032},
  doi       = {10.3390/healthcare11152139},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-362686},
  year      = {2023},
  abstract  = {In March 2020, Germany imposed a nationwide lockdown to curb the spread of COVID-19, prompting questions about the impact on the incidence of common fractures. This study examined 15 fracture types in pre-outbreak (2010-2019) and post-outbreak (2020-2021) periods, using data categorized by age (18-64 years, 65 years) and sex (male, female). Linear regression assessed annual growth rates, and mean fracture numbers were compared across periods for significant differences. Results indicated a positive correlation between fracture incidence rates and time for various types, such as cervical, thoracic, lumbar, and pelvic spine fractures, rib fractures, femoral neck, pertrochanteric femur, femoral shaft, and ankle fractures. Frequencies of proximal humerus, distal radius, femoral neck, pertrochanteric femur, femoral shaft, and ankle fractures in 2020 and 2021 were within predicted ranges from previous years. However, rib fractures and spinal fractures (cervical, thoracic, lumbar, and pelvic spine) occurred less frequently during this time. Notably, this study found a consistent decline in most fracture types for individuals aged 18-64 after the pandemic's onset, while the fracture incidence of hip fractures, often referred to as fragility fractures, for those over 65 remained unchanged. Fibula fractures showed the most considerable decrease in both age groups. In conclusion, the COVID-19 pandemic substantially impacted fracture incidence, with lower rates among individuals under 65 and unchanged fragility fractures in the elderly population.},
  language  = {en}
}
@article{EidmannHeinzOberfeldetal.2023,
  author    = {Eidmann, Annette and Heinz, Tizian and Oberfeld, Jan and Weißenberger, Manuel and Rudert, Maximilian and Stratos, Ioannis},
  title     = {Epidemiology and trends in cartilage surgery of the foot and ankle in Germany: an analysis of national healthcare billing and reporting data from 2006 to 2020},
  series = {Medicina},
  volume    = {59},
  journal   = {Medicina},
  number    = {7},
  issn      = {1648-9144},
  doi       = {10.3390/medicina59071256},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-362835},
  year      = {2023},
  abstract  = {Background and objectives: Cartilage surgery constitutes a standard intervention in foot and ankle procedures. Currently, there is a lack of epidemiological data on its frequency, age distribution, and surgical options for cartilage surgery. This study aimed to investigate the current landscape of cartilage surgery in Germany and identify the most common procedures from an epidemiological standpoint. Materials and methods: Medical billing and reporting data from the Federal Statistical Office of Germany, encompassing the period 2006-2020, was examined, including all foot and ankle cartilage surgical procedures (summarized under OPS codes 5-812 and 5-801). The dataset incorporated information on the affected joint, patient age and sex, and surgery type. Each surgical procedure was categorized as "debridement", "regeneration" or "refixation". Linear and nonlinear regression analyses were employed, with a statistical significance threshold of 0.05. Results: From the total of 136,501 procedures conducted during the study period, the most frequently performed interventions were microfracture (58,252) and chondroplasty (56,135), and thus, debridement procedures were in the leading position. The use of acellular membranes was the most used regenerative technique (n = 11,414). At the ankle joint, interventions were mostly arthroscopic and in men, while foot cartilage surgeries were preferably performed via open surgery and mostly in women. Age distribution analysis revealed two primary peaks: the first in the 20-25-year-old group (ankle and foot) and the second in the 45-50-year-old group (ankle) and 55-60-year-old group (foot). Refixation and regenerative procedures were more frequent among younger individuals, while debriding procedures were more frequent among older individuals. Regenerative procedures, particularly in the ankle, significantly increased over time. Conclusions: Cartilage surgery of the foot and ankle was common, with two primary age groups predominantly affected. Notably, recent years have witnessed a considerable rise in cartilage regenerative procedures.},
  language  = {en}
}
@article{BorojevićJaukovićKukoljetal.2022,
  author    = {Borojević, Ana and Jauković, Aleksandra and Kukolj, Tamara and Mojsilović, Slavko and Obradović, Hristina and Trivanović, Drenka and Živanović, Milena and Zečević, Željko and Simić, Marija and Gobeljić, Borko and Vujić, Dragana and Bugarski, Diana},
  title     = {Vitamin D3 stimulates proliferation capacity, expression of pluripotency markers, and osteogenesis of human bone marrow mesenchymal stromal/stem cells, partly through SIRT1 signaling},
  series = {Biomolecules},
  volume    = {12},
  journal   = {Biomolecules},
  number    = {2},
  issn      = {2218-273X},
  doi       = {10.3390/biom12020323},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-262203},
  year      = {2022},
  abstract  = {The biology of vitamin D3 is well defined, as are the effects of its active metabolites on various cells, including mesenchymal stromal/stem cells (MSCs). However, the biological potential of its precursor, cholecalciferol (VD3), has not been sufficiently investigated, although its significance in regenerative medicine — mainly in combination with various biomaterial matrices — has been recognized. Given that VD3 preconditioning might also contribute to the improvement of cellular regenerative potential, the aim of this study was to investigate its effects on bone marrow (BM) MSC functions and the signaling pathways involved. For that purpose, the influence of VD3 on BM-MSCs obtained from young human donors was determined via MTT test, flow cytometric analysis, immunocytochemistry, and qRT-PCR. Our results revealed that VD3, following a 5-day treatment, stimulated proliferation, expression of pluripotency markers (NANOG, SOX2, and Oct4), and osteogenic differentiation potential in BM-MSCs, while it reduced their senescence. Moreover, increased sirtuin 1 (SIRT1) expression was detected upon treatment with VD3, which mediated VD3-promoted osteogenesis and, partially, the stemness features through NANOG and SOX2 upregulation. In contrast, the effects of VD3 on proliferation, Oct4 expression, and senescence were SIRT1-independent. Altogether, these data indicate that VD3 has strong potential to modulate BM-MSCs' features, partially through SIRT1 signaling, although the precise mechanisms merit further investigation.},
  language  = {en}
}
@article{AchenbachKleinLuigetal.2021,
  author    = {Achenbach, Leonard and Klein, Christian and Luig, Patrick and Bloch, Hendrik and Schneider, Dominik and Fehske, Kai},
  title     = {Collision with opponents - but not foul play - dominates injury mechanism in professional men's basketball},
  series = {BMC Sports Science Medicine and Rehabilitation},
  volume    = {13},
  journal   = {BMC Sports Science Medicine and Rehabilitation},
  doi       = {10.1186/s13102-021-00322-z},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-261765},
  year      = {2021},
  abstract  = {Background To identify injury patterns and mechanisms in professional men's basketball by means of video match analysis. Methods In Germany, injuries are registered with the statutory accident insurance for professional athletes (VBG) by clubs or club physicians as part of occupational accident reporting. Moderate and severe injuries (absence of > 7 days) sustained during basketball competition in one of four seasons (2014-2017 and 2018-2019) in the first or second national men's league in Germany were prospectively analyzed using a newly developed standardized observation form. Season 2017-2018 was excluded because of missing video material. Results Video analysis included 175 (53\%) of 329 moderate and severe match injuries. Contact patterns categorized according to the different body sites yielded eight groups of typical injury patterns: one each for the head, shoulders, and ankles, two for the thighs, and three for the knees. Injuries to the head (92\%), ankles (76\%), shoulders (70\%), knees (47\%), and thighs (32\%) were mainly caused by direct contact. The injury proportion of foul play was 19\%. Most injuries (61\%) occurred in the central zone below the basket. More injuries occurred during the second (OR 1.8, p = 0.018) and fourth quarter (OR 1.8, p = 0.022) than during the first and third quarter of the match. Conclusion The eight identified injury patterns differed substantially in their mechanisms. Moderate and severe match injuries to the head, shoulders, knees, and ankles were mainly caused by collision with opponents and teammates. Thus, stricter rule enforcement is unlikely to facilitate safer match play.},
  language  = {en}
}
@article{SchmalzlPlumhoffGilbertetal.2019,
  author    = {Schmalzl, J. and Plumhoff, P. and Gilbert, F. and Gohlke, F. and Konrads, C. and Brunner, U. and Jakob, F. and Ebert, R. and Steinert, AF},
  title     = {The inflamed biceps tendon as a pain generator in the shoulder: A histological and biomolecular analysis},
  series = {Journal of Orthopaedic Surgery},
  volume    = {27},
  journal   = {Journal of Orthopaedic Surgery},
  number    = {1},
  doi       = {10.1177/2309499018820349},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-228611},
  pages     = {1-10},
  year      = {2019},
  abstract  = {Introduction: The long head of the biceps (LHB) is often resected in shoulder surgery. However, its contribution to inflammatory processes in the shoulder remains unclear. In the present study, inflamed and noninflamed human LHBs were comparatively characterized for features of inflammation. Materials and methods: Twenty-two resected LHB tendons were classified into inflamed (n = 11) and noninflamed (n = 11) samples. For histological examination, samples were stained with hematoxylin eosin, Azan, van Gieson, and Masson Goldner trichrome. Neuronal tissue was immunohistochemically visualized. In addition, specific inflammatory marker gene expression of primary LHB-derived cell cultures were analyzed. Results: Features of tendinopathy, such as collagen disorganization, infiltration by inflammatory cells, neovascularization, and extensive neuronal innervation were found in the tendinitis group. Compared to noninflamed samples, inflamed LHBs showed a significantly increased inflammatory marker gene expression Conclusion: Structural and biomolecular differences of both groups suggest that the LHB tendon acts as an important pain generator in the shoulder joint. These findings can, on the one hand, contribute to the understanding of the biomolecular genesis of LHB tendinitis and, on the other hand, provide possibilities for new therapeutic approaches.},
  language  = {en}
}
@article{ShekharHowellNedopiletal.2022,
  author    = {Shekhar, Adithya and Howell, Stephen M. and Nedopil, Alexander J. and Hull, Maury L.},
  title     = {Excellent and good results treating stiffness with early and late manipulation after unrestricted caliper-verified kinematically aligned TKA},
  series = {Journal of Personalized Medicine},
  volume    = {12},
  journal   = {Journal of Personalized Medicine},
  number    = {2},
  issn      = {2075-4426},
  doi       = {10.3390/jpm12020304},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-262094},
  year      = {2022},
  abstract  = {Manipulation under anesthesia (MUA) for stiffness within 6 to 12 weeks after mechanically aligned total knee arthroplasty (TKA) generally yields better outcome scores than an MUA performed later. However, the timing of MUA after unrestricted, caliper-verified, kinematically aligned (KA) TKA remains uncertain. A retrospective review identified 82 of 3558 (2.3\%) KA TKA patients treated with an MUA between 2010 and 2017. Thirty patients treated with an MUA within 3 months of the TKA (i.e., early) and 24 in the late group (i.e., >3 months) returned a questionnaire after a mean of 6 years and 5 years, respectively. Mean outcome scores for the early vs. late group were 78 vs. 62 for the Forgotten Joint Score (FJS) (p = 0.023) and 42 vs. 39 for the Oxford Knee Score (OKS) (p = 0.037). Subjectively, the early vs. late group responses indicated that 83\% vs. 67\% walked without a limp, 73\% vs. 54\% had normal extension, and 43\% vs. 25\% had normal flexion. An MUA within 3 months after unrestricted KA TKA provided excellent FJS and OKS at final follow-up relative to a late MUA. A late MUA performed after 3 months is worth consideration because of the good FJS and OKS scores, albeit with a risk of a persistent limp and limitation in knee extension and flexion.},
  language  = {en}
}
@article{WagenbrennerHeinzHorasetal.2020,
  author    = {Wagenbrenner, Mike and Heinz, Tizian and Horas, Konstantin and Jakuscheit, Axel and Arnholdt, J{\"o}rg and Hermann, Marietta and Rudert, Maximilian and Holzapfel, Boris M. and Steinert, Andre F. and Weißenberger, Manuel},
  title     = {The human arthritic hip joint is a source of mesenchymal stromal cells (MSCs) with extensive multipotent differentiation potential},
  series = {BMC Musculoskeletal Disorders},
  volume    = {21},
  journal   = {BMC Musculoskeletal Disorders},
  number    = {1},
  doi       = {10.1186/s12891-020-03340-z},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-229497},
  year      = {2020},
  abstract  = {Background While multiple in vitro studies examined mesenchymal stromal cells (MSCs) derived from bone marrow or hyaline cartilage, there is little to no data about the presence of MSCs in the joint capsule or the ligamentum capitis femoris (LCF) of the hip joint. Therefore, this in vitro study examined the presence and differentiation potential of MSCs isolated from the bone marrow, arthritic hyaline cartilage, the LCF and full-thickness samples of the anterior joint capsule of the hip joint. Methods MSCs were isolated and multiplied in adherent monolayer cell cultures. Osteogenesis and adipogenesis were induced in monolayer cell cultures for 21 days using a differentiation medium containing specific growth factors, while chondrogenesis in the presence of TGF-ss1 was performed using pellet-culture for 27 days. Control cultures were maintained for comparison over the same duration of time. The differentiation process was analyzed using histological and immunohistochemical stainings as well as semiquantitative RT-PCR for measuring the mean expression levels of tissue-specific genes. Results This in vitro research showed that the isolated cells from all four donor tissues grew plastic-adherent and showed similar adipogenic and osteogenic differentiation capacity as proven by the histological detection of lipid droplets or deposits of extracellular calcium and collagen type I. After 27 days of chondrogenesis proteoglycans accumulated in the differentiated MSC-pellets from all donor tissues. Immunohistochemical staining revealed vast amounts of collagen type II in all differentiated MSC-pellets, except for those from the LCF. Interestingly, all differentiated MSCs still showed a clear increase in mean expression of adipogenic, osteogenic and chondrogenic marker genes. In addition, the examination of an exemplary selected donor sample revealed that cells from all four donor tissues were clearly positive for the surface markers CD44, CD73, CD90 and CD105 by flow cytometric analysis. Conclusions This study proved the presence of MSC-like cells in all four examined donor tissues of the hip joint. No significant differences were observed during osteogenic or adipogenic differentiation depending on the source of MSCs used. Further research is necessary to fully determine the tripotent differentiation potential of cells isolated from the LCF and capsule tissue of the hip joint.},
  language  = {en}
}
@article{HowellGillSheltonetal.2022,
  author    = {Howell, Stephen M. and Gill, Manpreet and Shelton, Trevor J. and Nedopil, Alexander J.},
  title     = {Reoperations are few and confined to the most valgus phenotypes 4 years after unrestricted calipered kinematically aligned TKA},
  series = {Knee Surgery, Sports Traumatology, Arthroscopy},
  volume    = {30},
  journal   = {Knee Surgery, Sports Traumatology, Arthroscopy},
  number    = {3},
  doi       = {10.1007/s00167-021-06473-3},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-265291},
  pages     = {948-957},
  year      = {2022},
  abstract  = {Purpose The present study determined the postoperative phenotypes after unrestricted calipered kinematically aligned (KA) total knee arthroplasty (TKA), whether any phenotypes were associated with reoperation, implant revision, and lower outcome scores at 4 years, and whether the proportion of TKAs within each phenotype was comparable to those of the nonarthritic contralateral limb. Methods From 1117 consecutive primary TKAs treated by one surgeon with unrestricted calipered KA, an observer identified all patients (N = 198) that otherwise had normal paired femora and tibiae on a long-leg CT scanogram. In both legs, the distal femur-mechanical axis angle (FMA), proximal tibia-mechanical axis angle (TMA), and the hip-knee-ankle angle (HKA) were measured. Each alignment angle was assigned to one of Hirschmann's five FMA, five TMA, and seven HKA phenotype categories. Results Three TKAs (1.5\%) underwent reoperation for anterior knee pain or patellofemoral instability in the subgroup of patients with the more valgus phenotypes. There were no implant revisions for component loosening, wear, or tibiofemoral instability. The median Forgotten Joint Score (FJS) was similar between phenotypes. The median Oxford Knee Score (OKS) was similar between the TMA and HKA phenotypes and greatest in the most varus FMA phenotype. The phenotype proportions after calipered KA TKA were comparable to the contralateral leg. Conclusion Unrestricted calipered KA's restoration of the wide range of phenotypes did not result in implant revision or poor FJS and OKS scores at a mean follow-up of 4 years. The few reoperated patients had a more valgus setting of the prosthetic trochlea than recommended for mechanical alignment. Designing a femoral component specifically for KA that restores patellofemoral kinematics with all phenotypes, especially the more valgus ones, is a strategy for reducing reoperation risk.},
  language  = {en}
}
@article{KemmlerKohlJakobetal.2020,
  author    = {Kemmler, Wolfgang and Kohl, Matthias and Jakob, Franz and Engelke, Klaus and Stengel, Simon von},
  title     = {Effects of high intensity dynamic resistance exercise and whey protein supplements on osteosarcopenia in older men with low bone and muscle mass. Final results of the randomized controlled FrOST study},
  series = {Nutrients},
  volume    = {12},
  journal   = {Nutrients},
  number    = {8},
  issn      = {2072-6643},
  doi       = {10.3390/nu12082341},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-211108},
  year      = {2020},
  abstract  = {The present study aimed to evaluate the effect of high intensity dynamic resistance exercise (HIT-DRT) and whey protein supplementation (WPS) on bone mineral density (BMD) and sarcopenia parameters in osteosarcopenic men. Men ≥ 72 years with osteosarcopenia (n = 43) were randomly assigned to a HIT-RT (HIT-RT: n = 21) or a non-training control group (n = 22). Supervised HIT-RT twice/week was applied for 18 months, while the control group maintained their habitual lifestyle. Supplying WPS, total protein intake amounted to 1.5-1.6 (HIT-RT) and 1.2 g/kg/body mass/d (control). Both groups were supplied with calcium and vitamin D. Primary study outcomes were BMD and the sarcopenia Z-score. After adjusting for multiplicity, we observed significant positive effects for sarcopenia Z-score (standardized mean difference (SMD): 1.40), BMD at lumbar spine (SMD: 0.72) and total hip (SMD: 0.72). In detail, effect sizes for skeletal muscle mass changes were very pronounced (1.97, p < 0.001), while effects for functional sarcopenia parameters were moderate (0.87, p = 0.008; handgrip strength) or low (0.39, p = 0.209; gait velocity). Apart from one man who reported short periods of temporary worsening of existing joint pain, no HIT-RT/WPS-related adverse effects or injuries were reported. We consider HIT-RT supported by whey protein supplementation as a feasible, attractive, safe and highly effective option to fight osteosarcopenia in older men.},
  language  = {en}
}
@article{KrsticHerrmannGadjanskietal.2017,
  author    = {Krstic, Jelena and Herrmann, Marietta and Gadjanski, Ivana and Mojsilovic, Slavko},
  title     = {Editorial: Microenvironment-derived stem cell plasticity},
  series = {Frontiers in Cell and Developmental Biology},
  volume    = {5},
  journal   = {Frontiers in Cell and Developmental Biology},
  issn      = {2296-634X},
  doi       = {10.3389/fcell.2017.00082},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-197424},
  year      = {2017},
  abstract  = {No abstract available.},
  language  = {en}
}
@article{HerrmannEngelkeEbertetal.2020,
  author    = {Herrmann, Marietta and Engelke, Klaus and Ebert, Regina and M{\"u}ller-Deubert, Sigrid and Rudert, Maximilian and Ziouti, Fani and Jundt, Franziska and Felsenberg, Dieter and Jakob, Franz},
  title     = {Interactions between muscle and bone — Where physics meets biology},
  series = {Biomolecules},
  volume    = {10},
  journal   = {Biomolecules},
  number    = {3},
  issn      = {2218-273X},
  doi       = {10.3390/biom10030432},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-203399},
  year      = {2020},
  abstract  = {Muscle and bone interact via physical forces and secreted osteokines and myokines. Physical forces are generated through gravity, locomotion, exercise, and external devices. Cells sense mechanical strain via adhesion molecules and translate it into biochemical responses, modulating the basic mechanisms of cellular biology such as lineage commitment, tissue formation, and maturation. This may result in the initiation of bone formation, muscle hypertrophy, and the enhanced production of extracellular matrix constituents, adhesion molecules, and cytoskeletal elements. Bone and muscle mass, resistance to strain, and the stiffness of matrix, cells, and tissues are enhanced, influencing fracture resistance and muscle power. This propagates a dynamic and continuous reciprocity of physicochemical interaction. Secreted growth and differentiation factors are important effectors of mutual interaction. The acute effects of exercise induce the secretion of exosomes with cargo molecules that are capable of mediating the endocrine effects between muscle, bone, and the organism. Long-term changes induce adaptations of the respective tissue secretome that maintain adequate homeostatic conditions. Lessons from unloading, microgravity, and disuse teach us that gratuitous tissue is removed or reorganized while immobility and inflammation trigger muscle and bone marrow fatty infiltration and propagate degenerative diseases such as sarcopenia and osteoporosis. Ongoing research will certainly find new therapeutic targets for prevention and treatment.},
  language  = {en}
}
@phdthesis{Braag2022,
  author    = {Braag, Aaron},
  title     = {10 Jahres Ergebnisse nach muskelschonendem modifiziertem Watson Jones Zugang bei der Implantation von H{\"u}fttotalendoprothesen},
  doi       = {10.25972/OPUS-28141},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-281416},
  school      = {Universit{\"a}t W{\"u}rzburg},
  year      = {2022},
  abstract  = {Bei der Implantation von H{\"u}fttotalendoprothesen (HTEP) finden seit etwa 15 Jahren minimalinvasive muskelschonende Zug{\"a}nge zunehmend Verwendung. Langfristige Daten der Zug{\"a}nge, insbesondere des minimalinvasiven anterolateralen Zuganges nach Watson-Jones (ALMI) sind in der Literatur bisher nur unzureichend vorhanden. Methodik: Ziel dieser Studie war es ein Kollektiv nach HTEP Implantation mit ALMI Zugang mit einem Kollektiv nach HTEP Implantation mit lateralem Zugang nach 10 Jahren hinsichtlich Gelenksfunktion, Muskelfunktion, Zufriedenheit und radiologischer Parameter zu vergleichen und etwaige Unterschiede in der Langzeitbilanz zu detektieren. Zwei Kollektive mit jeweils 29 operierten H{\"u}ftgelenken, Erstimplantation durch die gleichen Operateure in den Jahren 2005 bis 2008, wurden im Diakoniewerk M{\"u}nchen-Maxvorstadt nachuntersucht. Die daf{\"u}r herangezogenen Parameter waren Harris Hip Score, Forgotten Joint Score-12, klinische Pr{\"u}fung des Trendelenburg Zeichens, postoperative R{\"o}ntgenbildgebung, Auftreten von Komplikationen und Narbenl{\"a}nge. Ergebnisse \& Schlussfolgerungen: Die beiden Kollektive zeigten in den Parametern Harris Hip Score, Forgotten Joint Score und klinische Pr{\"u}fung des Trendelenburg Zeichens geringf{\"u}gige Unterschiede zugunsten des ALMI Kollektivs, die jedoch nicht signifikant waren. Beide Kollektive erreichten in den beschriebenen Scores sehr gute bis exzellente Ergebnisse nach 10 Jahren. Das geringere Auftreten eines auff{\"a}lligen Trendelenburg Zeichens im ALMI Kollektiv (13,8 vs. 6,9 \%) gibt Hinweise auf eine verbesserte Funktion der Glutealmuskulatur durch die intraoperative Muskelschonung. Die beiden Zug{\"a}nge zeigten in den radiologischen Parametern und der Komplikationsrate ebenb{\"u}rtige Ergebnisse. Vermehrte Fehlpositionierungen wurden im ALMI Kollektiv nicht beobachtet. Unsere Beobachtungen passen zu den wenigen vorhandenen in der Literatur beschriebenen Ergebnissen von minimalinvasiven muskelschonenden Zug{\"a}ngen in der Langzeitbilanz.},
  subject      = {Minimalinvasiv},
  language  = {de}
}