@article{AdelfingerBesslerCeciletal.2015,
  author    = {Adelfinger, Marion and Bessler, Simon and Cecil, Alexander and Langbein-Laugwitz, Johanna and Frentzen, Alexa and Gentschev, Ivaylo and Szalay, Aladar A.},
  title     = {Preclinical Testing Oncolytic Vaccinia Virus Strain GLV-5b451 Expressing an Anti-VEGF Single-Chain Antibody for Canine Cancer Therapy},
  series = {Viruses},
  volume    = {7},
  journal   = {Viruses},
  doi       = {10.3390/v7072811},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-125705},
  pages     = {4075-4092},
  year      = {2015},
  abstract  = {Virotherapy on the basis of oncolytic vaccinia virus (VACV) strains is a novel approach for canine cancer therapy. Here we describe, for the first time, the characterization and the use of VACV strain GLV-5b451 expressing the anti-vascular endothelial growth factor (VEGF) single-chain antibody (scAb) GLAF-2 as therapeutic agent against different canine cancers. Cell culture data demonstrated that GLV-5b451 efficiently infected and destroyed all four tested canine cancer cell lines including: mammary carcinoma (MTH52c), mammary adenoma (ZMTH3), prostate carcinoma (CT1258), and soft tissue sarcoma (STSA-1). The GLV-5b451 virus-mediated production of GLAF-2 antibody was observed in all four cancer cell lines. In addition, this antibody specifically recognized canine VEGF. Finally, in canine soft tissue sarcoma (CSTS) xenografted mice, a single systemic administration of GLV-5b451 was found to be safe and led to anti-tumor effects resulting in the significant reduction and substantial long-term inhibition of tumor growth. A CD31-based immuno-staining showed significantly decreased neo-angiogenesis in GLV-5b451-treated tumors compared to the controls. In summary, these findings indicate that GLV-5b451 has potential for use as a therapeutic agent in the treatment of CSTS.},
  language  = {en}
}
@article{AdolfiCarreiraJesusetal.2015,
  author    = {Adolfi, Mateus C. and Carreira, Ana C. O. and Jesus, L{\´a}zaro W. O. and Bogerd, Jan and Funes, Rejane M. and Schartl, Manfred and Sogayar, Mari C. and Borella, Maria I.},
  title     = {Molecular cloning and expression analysis of dmrt1 and sox9 during gonad development and male reproductive cycle in the lambari fish, Astyanax altiparanae},
  series = {Reproductive Biology and Endocrinology},
  volume    = {13},
  journal   = {Reproductive Biology and Endocrinology},
  number    = {2},
  doi       = {10.1186/1477-7827-13-2},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-126486},
  year      = {2015},
  abstract  = {Background The dmrt1 and sox9 genes have a well conserved function related to testis formation in vertebrates, and the group of fish presents a great diversity of species and reproductive mechanisms. The lambari fish (Astyanax altiparanae) is an important Neotropical species, where studies on molecular level of sex determination and gonad maturation are scarce. Methods Here, we employed molecular cloning techniques to analyze the cDNA sequences of the dmrt1 and sox9 genes, and describe the expression pattern of those genes during development and the male reproductive cycle by qRT-PCR, and related to histology of the gonad. Results Phylogenetic analyses of predicted amino acid sequences of dmrt1 and sox9 clustered A. altiparanae in the Ostariophysi group, which is consistent with the morphological phylogeny of this species. Studies of the gonad development revealed that ovary formation occurred at 58 days after hatching (dah), 2 weeks earlier than testis formation. Expression studies of sox9 and dmrt1 in different tissues of adult males and females and during development revealed specific expression in the testis, indicating that both genes also have a male-specific role in the adult. During the period of gonad sex differentiation, dmrt1 seems to have a more significant role than sox9. During the male reproductive cycle dmrt1 and sox9 are down-regulated after spermiation, indicating a role of these genes in spermatogenesis. Conclusions For the first time the dmrt1 and sox9 were cloned in a Characiformes species. We show that both genes have a conserved structure and expression, evidencing their role in sex determination, sex differentiation and the male reproductive cycle in A. altiparanae. These findings contribute to a better understanding of the molecular mechanisms of sex determination and differentiation in fish.},
  language  = {en}
}
@article{AndronicShirakashiPickeletal.2015,
  author    = {Andronic, Joseph and Shirakashi, Ryo and Pickel, Simone U. and Westerling, Katherine M. and Klein, Teresa and Holm, Thorge and Sauer, Markus and Sukhorukov, Vladimir L.},
  title     = {Hypotonic Activation of the Myo-Inositol Transporter SLC5A3 in HEK293 Cells Probed by Cell Volumetry, Confocal and Super-Resolution Microscopy},
  series = {PLoS One},
  volume    = {10},
  journal   = {PLoS One},
  number    = {3},
  doi       = {10.1371/journal.pone.0119990},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-126408},
  year      = {2015},
  abstract  = {Swelling-activated pathways for myo-inositol, one of the most abundant organic osmolytes in mammalian cells, have not yet been identified. The present study explores the SLC5A3 protein as a possible transporter of myo-inositol in hyponically swollen HEK293 cells. To address this issue, we examined the relationship between the hypotonicity-induced changes in plasma membrane permeability to myo-inositol Pino [m/s] and expression/localization of SLC5A3. Pino values were determined by cell volumetry over a wide tonicity range (100-275 mOsm) in myo-inositol-substituted solutions. While being negligible under mild hypotonicity (200-275 mOsm), Pino grew rapidly at osmolalities below 200 mOsm to reach a maximum of ∼3 nm/s at 100-125 mOsm, as indicated by fast cell swelling due to myo-inositol influx. The increase in Pino resulted most likely from the hypotonicity-mediated incorporation of cytosolic SLC5A3 into the plasma membrane, as revealed by confocal fluorescence microscopy of cells expressing EGFP-tagged SLC5A3 and super-resolution imaging of immunostained SLC5A3 by direct stochastic optical reconstruction microscopy (dSTORM). dSTORM in hypotonic cells revealed a surface density of membrane-associated SLC5A3 proteins of 200-2000 localizations/μm2. Assuming SLC5A3 to be the major path for myo-inositol, a turnover rate of 80-800 myo-inositol molecules per second for a single transporter protein was estimated from combined volumetric and dSTORM data. Hypotonic stress also caused a significant upregulation of SLC5A3 gene expression as detected by semiquantitative RT-PCR and Western blot analysis. In summary, our data provide first evidence for swelling-mediated activation of SLC5A3 thus suggesting a functional role of this transporter in hypotonic volume regulation of mammalian cells.},
  language  = {en}
}
@article{BaurSchedelbeckPulzeretal.2015,
  author    = {Baur, Johannes and Schedelbeck, Ulla and Pulzer, Alina and Bluemel, Christina and Wild, Vanessa and Fassnacht, Martin and Steger, U.},
  title     = {A case report of a solitary pancreatic metastasis of an adrenocortical carcinoma},
  series = {BMC Surgery},
  volume    = {15},
  journal   = {BMC Surgery},
  number    = {93},
  doi       = {10.1186/s12893-015-0076-3},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-126130},
  year      = {2015},
  abstract  = {Background Solitary metastases to the pancreas are rare. Therefore the value of resection in curative intention remains unclear. In the literature there are several promising reports about resection of solitary metastasis to the pancreas mainly of renal origin. Case presentation Here we report for the first time on the surgical therapy of a 1.5 cm solitary pancreatic metastasis of an adrenocortical carcinoma. The metastasis occurred almost 6 years after resection of the primary tumor. A partial pancreatoduodenectomy was performed and postoperatively adjuvant mitotane treatment was initiated. During the follow-up of 3 years after surgery no evidence of tumor recurrence occurred. Conclusion Resection of pancreatic tumors should be considered, even if the mass is suspicious for metastatic disease including recurrence of adrenocortical cancer.},
  language  = {en}
}
@article{BoelchJansenMeffertetal.2015,
  author    = {Boelch, S. P. and Jansen, H. and Meffert, R. H. and Frey, S. P.},
  title     = {Six Sesamoid Bones on Both Feet: Report of a Rare Case},
  series = {Journal of Clinical and Diagnostic Research},
  volume    = {9},
  journal   = {Journal of Clinical and Diagnostic Research},
  number    = {8},
  doi       = {10.7860/JCDR/2015/12842.6394},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-126073},
  pages     = {RD04-RD05},
  year      = {2015},
  abstract  = {There is a variation of the total number of distinct bones in the human in the literature. This difference is mainly caused by the variable existence of sesamoid bones. Sesamoid bones at the first MTP are seen regularly. In contrast additional sesamoid bones at the divond to fifth MTP are rare. We report a case of additional sesamoid bones at every metatarsophalangeal joint (MTP) of both feet. A 22-year-old female Caucasian presented with weight-dependent pain of the divond MTP of the left foot. In the radiographs of both feet additional sesamoid bones at every MTP could be seen. This case reports a very rare variation in human anatomy. A similar case has not been displayed to the academic society and therefore should be acknowledged.},
  language  = {en}
}
@article{BoelchBarthelGoebeletal.2015,
  author    = {Boelch, Sebastian Philipp and Barthel, Thomas and Goebel, Sascha and Rudert, Maximilian and Plumhoff, Piet},
  title     = {Calcinosis universalis - a rare case with classical presentation},
  series = {Case Reports in Orthopedics},
  volume    = {2015},
  journal   = {Case Reports in Orthopedics},
  doi       = {10.1155/2015/505420},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-126300},
  pages     = {505420},
  year      = {2015},
  abstract  = {Juvenile Dermatomyositis (JDM) is a rare autoimmune disease in children and adolescents. In these patients calcinosis might be the most characteristic symptom. However there are only few reported cases of intramuscular calcinosis in Dermatomyositis. We report a case of calcinosis universalis (CU) of the elbow in JDM successfully treated with broaching. The patient, a 24-year-old woman, suffered from a long history of JDM. On examination she presented with a fistula lateral to the olecranon and pain of the right elbow joint. Plain X-rays displayed a diffuse pattern of multiple periarticular, subcutaneous, and intramuscular calcifications. The patient underwent surgery for histological and microbiological sampling as well as broaching. Intraoperatively sinus formation and subfascial hard calcium deposition were found. Due to the risk of collateral tissue damage, incomplete broaching was performed. A local infection with Staphylococcus was diagnosed and treated with antibiotics. On six-week and 30-month follow-up the patient was free of pain and had very good function. Calcifications on standard radiographs had almost resolved entirely. This case report gives a summary on calcinosis in Dermatomyositis and adds a new case of recalcitrant CU to the literature. Broaching surgery proved to be a reliable treatment option in symptomatic calcinosis.},
  language  = {en}
}
@article{BoivinBeyersdorfPalmetal.2015,
  author    = {Boivin, Val{\´e}rie and Beyersdorf, Niklas and Palm, Dieter and Nikolaev, Viacheslav O. and Schlipp, Angela and M{\"u}ller, Justus and Schmidt, Doris and Kocoski, Vladimir and Kerkau, Thomas and H{\"u}nig, Thomas and Ertl, Georg and Lohse, Martin J. and Jahns, Roland},
  title     = {Novel Receptor-Derived Cyclopeptides to Treat Heart Failure Caused by \(Anti-β_1-Adrenoceptor\) Antibodies in a Human-Analogous Rat Model},
  series = {PLoS One},
  volume    = {10},
  journal   = {PLoS One},
  number    = {2},
  doi       = {10.1371/journal.pone.0117589},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-126028},
  pages     = {e0117589},
  year      = {2015},
  abstract  = {Despite recent therapeutic advances the prognosis of heart failure remains poor. Recent research suggests that heart failure is a heterogeneous syndrome and that many patients have stimulating auto-antibodies directed against the second extracellular loop of the \(β_1\) adrenergic receptor \((β_1EC2)\). In a human-analogous rat model such antibodies cause myocyte damage and heart failure. Here we used this model to test a novel antibody-directed strategy aiming to prevent and/or treat antibody-induced cardiomyopathy. To generate heart failure, we immunised n = 76/114 rats with a fusion protein containing the human β1EC2 (amino-acids 195-225) every 4 weeks; n = 38/114 rats were control-injected with 0.9\% NaCl. Intravenous application of a novel cyclic peptide mimicking \(β_1EC2\) (\(β_1EC2-CP\), 1.0 mg/kg every 4 weeks) or administration of the \(β_1-blocker\) bisoprolol (15 mg/kg/day orally) was initiated either 6 weeks (cardiac function still normal, prevention-study, n = 24 (16 treated vs. 8 untreated)) or 8.5 months after the 1st immunisation (onset of cardiomyopathy, therapy-study, n = 52 (40 treated vs. 12 untreated)); n = 8/52 rats from the therapy-study received \(β_1EC2-CP/bisoprolol\) co-treatment. We found that \(β_1EC2-CP\) prevented and (alone or as add-on drug) treated antibody-induced cardiac damage in the rat, and that its efficacy was superior to mono-treatment with bisoprolol, a standard drug in heart failure. While bisoprolol mono-therapy was able to stop disease-progression, \(β_1EC2-CP\) mono-therapy -or as an add-on to bisoprolol- almost fully reversed antibody-induced cardiac damage. The cyclo¬peptide acted both by scavenging free \(anti-β_1EC2-antibodies\) and by targeting \(β_1EC2\)-specific memory B-cells involved in antibody-production. Our model provides the basis for the clinical translation of a novel double-acting therapeutic strategy that scavenges harmful \(anti-β_1EC2-antibodies\) and also selectively depletes memory B-cells involved in the production of such antibodies. Treatment with immuno-modulating cyclopeptides alone or as an add-on to \(β_1\)-blockade represents a promising new therapeutic option in immune-mediated heart failure.},
  language  = {en}
}
@article{BratengeierHolubyev2015,
  author    = {Bratengeier, Klaus and Holubyev, Kostyantyn},
  title     = {Characteristics of non-coplanar IMRT in the presence of target-embedded organs at risk},
  series = {Radiation Oncology},
  volume    = {10},
  journal   = {Radiation Oncology},
  number    = {207},
  doi       = {10.1186/s13014-015-0494-5},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-125292},
  year      = {2015},
  abstract  = {Background The aim is to analyze characteristics and to study the potentials of non-coplanar intensity modulated radiation therapy (IMRT) techniques. The planning study applies to generalized organ at risk (OAR) - planning target volume (PTV) geometries. Methods The authors focus on OARs embedded in the PTV. The OAR shapes are spherically symmetric (A), cylindrical (B), and bended (C). Several IMRT techniques are used for the planning study: a) non-coplanar quasi-isotropic; b) two sets of equidistant coplanar beams, half of beams incident in a plane perpendicular to the principal plane; c) coplanar equidistant (reference); d) coplanar plus one orthogonal beam. The number of beam directions varies from 9 to 16. The orientation of the beam sets is systematically changed; dose distributions resulting from optimal fluence are explored. A selection of plans is optimized with direct machine parameter optimization (DMPO) allowing 120 and 64 segments. The overall plan quality, PTV coverage, and OAR sparing are evaluated. Results For all fluence based techniques in cases A and C, plan quality increased considerably if more irradiation directions were used. For the cylindrically symmetric case B, however, only a weak beam number dependence was observed for the best beam set orientation, for which non-coplanar directions could be found where OAR- and PTV-projections did not overlap. IMRT plans using quasi-isotropical distributed non-coplanar beams showed stable results for all topologies A, B, C, as long as 16 beams were chosen; also the most unfavorable beam arrangement created results of similar quality as the optimally oriented coplanar configuration. For smaller number of beams or application in the trunk, a coplanar technique with additional orthogonal beam could be recommended. Techniques using 120 segments created by DMPO could qualitatively reproduce the fluence based results. However, for a reduced number of segments the beam number dependence declined or even reversed for the used planning system and the plan quality degraded substantially. Conclusions Topologies with targets encompassing sensitive OAR require sufficient number of beams of 15 or more. For the subgroup of topologies where beam incidences are possible which cover the whole PTV without direct OAR irradiation, the quality dependence on the number of beams is much less pronounced above 9 beams. However, these special non-coplanar beam directions have to be found. On the basis of this work the non-coplanar IMRT techniques can be chosen for further clinical planning studies.},
  language  = {en}
}
@article{BrieseSaalAppenzelleretal.2015,
  author    = {Briese, Michael and Saal, Lena and Appenzeller, Silke and Moradi, Mehri and Baluapuri, Apoorva and Sendtner, Michael},
  title     = {Whole transcriptome profiling reveals the RNA content of motor axons},
  series = {Nucleic Acids Research},
  journal   = {Nucleic Acids Research},
  doi       = {10.1093/nar/gkv1027},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-126800},
  year      = {2015},
  abstract  = {Most RNAs within polarized cells such as neurons are sorted subcellularly in a coordinated manner. Despite advances in the development of methods for profiling polyadenylated RNAs from small amounts of input RNA, techniques for profiling coding and non-coding RNAs simultaneously are not well established. Here, we optimized a transcriptome profiling method based on double-random priming and applied it to serially diluted total RNA down to 10 pg. Read counts of expressed genes were robustly correlated between replicates, indicating that the method is both reproducible and scalable. Our transcriptome profiling method detected both coding and long non-coding RNAs sized >300 bases. Compared to total RNAseq using a conventional approach our protocol detected 70\% more genes due to reduced capture of ribosomal RNAs. We used our method to analyze the RNA composition of compartmentalized motoneurons. The somatodendritic compartment was enriched for transcripts with post-synaptic functions as well as for certain nuclear non-coding RNAs such as 7SK. In axons, transcripts related to translation were enriched including the cytoplasmic non-coding RNA 7SL. Our profiling method can be applied to a wide range of investigations including perturbations of subcellular transcriptomes in neurodegenerative diseases and investigations of microdissected tissue samples such as anatomically defined fiber tracts.},
  language  = {en}
}
@article{BrillMeyerRoessler2015,
  author    = {Brill, Martin F. and Meyer, Anneke and Roessler, Wolfgang},
  title     = {It takes two—coincidence coding within the dual olfactory pathway of the honeybee},
  series = {Frontiers in Physiology},
  volume    = {6},
  journal   = {Frontiers in Physiology},
  number    = {208},
  doi       = {10.3389/fphys.2015.00208},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-126179},
  year      = {2015},
  abstract  = {To rapidly process biologically relevant stimuli, sensory systems have developed a broad variety of coding mechanisms like parallel processing and coincidence detection. Parallel processing (e.g., in the visual system), increases both computational capacity and processing speed by simultaneously coding different aspects of the same stimulus. Coincidence detection is an efficient way to integrate information from different sources. Coincidence has been shown to promote associative learning and memory or stimulus feature detection (e.g., in auditory delay lines). Within the dual olfactory pathway of the honeybee both of these mechanisms might be implemented by uniglomerular projection neurons (PNs) that transfer information from the primary olfactory centers, the antennal lobe (AL), to a multimodal integration center, the mushroom body (MB). PNs from anatomically distinct tracts respond to the same stimulus space, but have different physiological properties, characteristics that are prerequisites for parallel processing of different stimulus aspects. However, the PN pathways also display mirror-imaged like anatomical trajectories that resemble neuronal coincidence detectors as known from auditory delay lines. To investigate temporal processing of olfactory information, we recorded PN odor responses simultaneously from both tracts and measured coincident activity of PNs within and between tracts. Our results show that coincidence levels are different within each of the two tracts. Coincidence also occurs between tracts, but to a minor extent compared to coincidence within tracts. Taken together our findings support the relevance of spike timing in coding of olfactory information (temporal code).},
  language  = {en}
}