Dokument-ID Dokumenttyp Verfasser/Autoren Herausgeber Haupttitel Abstract Auflage Verlagsort Verlag Erscheinungsjahr Seitenzahl Schriftenreihe Titel Schriftenreihe Bandzahl ISBN Quelle der Hochschulschrift Konferenzname Quelle:Titel Quelle:Jahrgang Quelle:Heftnummer Quelle:Erste Seite Quelle:Letzte Seite URN DOI Abteilungen OPUS4-5084 Wissenschaftlicher Artikel Kreft, Jürgen; Berger, Harald; Härtlein, Michael; Müller, Bodo; Weidinger, Gerhard; Goebel, Werner Cloning and expression in Escherichia coli and Bacillus subtilis of the hemolysin (cereolysin) determinant from Bacillus cereus From a cosmid gene bank of Bacillus cereus GP4 in Escherichia coli we isolated clones which, after several days of incubation, formed hemolysis zones on erythrocyte agar plates. These clones contained recombinant cosmids with B. cereus DNA insertions of varying lengths which shared some common restriction fragments. The smallest insertionwas recloned as aPstl fragment into pJKK3-1, a shuttle vector which repücates in Bacillus subtilis and E. coli. When this recombinant plasmid (pJKK3-1 hly-1) was transformed into E. coli, it caused hemolysis on erythrocyte agar plates, but in liquid assays no extemal or intemal hemolytic activity could be detected with the E. coli transformants. B. subtilis carrying the same plasmid exhibited hemolytic activity at Ievels comparable to those ofthe B. cereus donor strain. The hemolysin produced in B. subtilis seemed to be indistinguishable from cereolysin in its sensitivity to cholesterol, activation by dithiothreitol, and inactivation by antibodies raised against cereolysin. When the recombinant DNA carrying the cereolysin gene was used as a probe in hybridization experiments with chromosomal DNA from a streptolysin 0-producing strain of Streptococcus pyogenes or from üsteriolysin-producing strains of Usteria monoeytogenes, no positive hybridization signals were obtained. These data soggest that the genes for these three SH-activated cytolysins do not have extended sequence homology. 1983 urn:nbn:de:bvb:20-opus-60596 Theodor-Boveri-Institut für Biowissenschaften OPUS4-5094 Wissenschaftlicher Artikel Härtlein, Michael; Schiessl, Sigrid; Wagner, Wilma; Rdest, Ursula; Kreft, Jürgen; Goebel, Werner Transport of hemolysin by Escherichia coli No abstract available 1983 urn:nbn:de:bvb:20-opus-60619 Theodor-Boveri-Institut für Biowissenschaften OPUS4-5095 Wissenschaftlicher Artikel Goebel, Werner; Kreft, Jürgen Accumulation of replicative intermediates and catenated forms of the colicinogenic factor E\(_1\) in E. coli during the replication at elevated temperatures No abstract available 1972 urn:nbn:de:bvb:20-opus-60625 Theodor-Boveri-Institut für Biowissenschaften OPUS4-6655 Wissenschaftlicher Artikel Linsenmair, Karl Eduard Anemomenotactic orientation in beetles and scorpions Scorpions, living in North African semideserts are - in spite of disrupting experimental interferences - able to maintain a certain direction in their natural environment in the dark on a plane surface. Under comparable laboratory conditions, excluding the possibility of light or gravity orientation, they can orient themselves if a directed air current passes over the "arena." In most cases the scorpions do not run necessarily with or against the wind, but rather maintain constant angles to the air current for anywhere from minutes to many hours. They are running anemomenotactically (ref. 1). Under identical conditions many species of beetles also orient themselves to air currents (refs. 2 to 4). The main problems to be solved in the study of anemomenotactic orientation are: (1) Which physical qualities of the air current have an influence on the anemomenotaxis? (2) With which sense organs do beetles and scorpions perceive wind directions? (3) Which physiological mechanism is the basis of anemomenotactic orientation? (4) What is the biological significance of anemomenotaxis in beetles and scorpions? With respect to these problems, more study has been done on beetles than on scorpions. Therefore, due to lack of space, I shall discuss mainly some of the results obtained in experiments with dung beetles (Geotrupes silvaticus, G. ,Stercorarius, G. armifrons, G. niger, Scarabaeus variolosus) and tenebrionid beetles (Tenebrio molitor, Pimelia grossa, P. tenuicomis, Scaurus dubius). 1972 urn:nbn:de:bvb:20-opus-78118 Theodor-Boveri-Institut für Biowissenschaften