Dokument-ID Dokumenttyp Verfasser/Autoren Herausgeber Haupttitel Abstract Auflage Verlagsort Verlag Erscheinungsjahr Seitenzahl Schriftenreihe Titel Schriftenreihe Bandzahl ISBN Quelle der Hochschulschrift Konferenzname Quelle:Titel Quelle:Jahrgang Quelle:Heftnummer Quelle:Erste Seite Quelle:Letzte Seite URN DOI Abteilungen OPUS4-22845 Wissenschaftlicher Artikel Scheer, Ulrich Boveri's research at the Zoological Station Naples: Rediscovery of his original microscope slides at the University of Würzburg Eric Davidson once wrote about Theodor Boveri: "From his own researches, and perhaps most important, his generalized interpretations, derive the paradigms that underlie modern inquiries into the genomic basis of embryogenesis" (Davidson, 1985). As luck would have it, the "primary data" of Boveri's experimental work, namely the microscope slides prepared by him and his wife Marcella during several stays at the Zoological Station in Naples (1901/02, 1911/12 and 1914), have survived at the University of Wurzburg. More than 600 slides exist and despite their age they are in a surprisingly good condition. The slides are labelled and dated in Boveri's handwriting and thus can be assigned to his published experimental work on sea urchin development. The results allowed Boveri to unravel the role of the cell nucleus and its chromosomes in development and inheritance. Here, I present an overview of the slides in the context of Boveri's work along with photographic images of selected specimens taken from the original slides. It is planned to examine the slides in more detail, take high-resolution focal image series of significant specimens and make them online available. 2018 1-8 Marine Genomics 40 urn:nbn:de:bvb:20-opus-228453 10.1016/j.margen.2018.01.003 Theodor-Boveri-Institut für Biowissenschaften OPUS4-3373 Konferenzveröffentlichung Dabauvalle, M.-C.; Wilken, N.; Ewald, A.; Kuhbier, A.; Senécal, J.-L.; Scheer, Ulrich Nuclear pore complex structure analyzed by immunogold EM with human autoantibodies No abstract available 1994 urn:nbn:de:bvb:20-opus-39439 Theodor-Boveri-Institut für Biowissenschaften OPUS4-2635 Wissenschaftlicher Artikel Scheer, Ulrich; Weisenberger, Dieter The nucleolus No abstract available 1994 urn:nbn:de:bvb:20-opus-32037 Theodor-Boveri-Institut für Biowissenschaften OPUS4-6850 Wissenschaftlicher Artikel Scheer, Ulrich Harold Garnet Callan 1917-1993 Professor Harold Gamet Callan, honorary member of the German Society for Cell Biology, died on the 3rd November 1993, at the age of 76. His name is inseparably connected with lampbrush chromosomes, the most spectacular and aesthetically ailuring form of chromosomes, which occupied the major part of his scientific career. " Mick" Callan's pioneering studies led to fruitful new concepts, served as a building block for many subsequent studies by others, and contributed enormously to our current understanding of chromosome organization and activity ... 1994 urn:nbn:de:bvb:20-opus-80789 Theodor-Boveri-Institut für Biowissenschaften OPUS4-2714 Wissenschaftlicher Artikel Wilken, Norbert; Kossner, Ursula; Senécal, Jean-Luc; Scheer, Ulrich; Dabauvalle, Marie-Christine Nup180, a novel nuclear pore complex protein localizing to the cytoplasmic ring and associated fibrils No abstract available 1993 urn:nbn:de:bvb:20-opus-32049 Theodor-Boveri-Institut für Biowissenschaften OPUS4-2676 Review Scheer, Ulrich; Thiry, Marc; Goessens, Guy Structure, function and assembly of the nucleolus No abstract available 1993 urn:nbn:de:bvb:20-opus-32057 Theodor-Boveri-Institut für Biowissenschaften OPUS4-3353 Wissenschaftlicher Artikel Fischer, Dagmar; Hock, Robert; Scheer, Ulrich DNA Topoisomerase II is not detectable on lampbrush chromosomes but enriched in the amplified nucleoli of xenopus oocytes In somatic cells DNA topoisomerase II (topo II) is thought to be involved in the domain Organization of the genome by anchoring the basis of chromatin loops to a chromosomal scafFold. Lampbrush chromosomes of am-phibian oocytes directly display this radial loop Organization in cytological preparations. In order to find out whether topo II may play a role in the Organization of these meiotic chromosomes, we performed immunofluorescence studies using antibodies against Xenopus topo II. Our results indicate that topo II is apparently absent from lampbrush chromosomes and is hence unlikely to act as a "fastener" of the numerous lateral chromosomal loops. Topo II was, however, enriched in the amplified nucleoli of Xenopus oocytes. 1993 urn:nbn:de:bvb:20-opus-32654 Theodor-Boveri-Institut für Biowissenschaften OPUS4-3559 Wissenschaftlicher Artikel Weisenberger, Dieter; Scheer, Ulrich; Benavente, Ricardo The DNA topoisomerase I inhibitor camptothecin blocks postmitotic reformation of nucleoli in mammmalian cells No abstract available 1993 urn:nbn:de:bvb:20-opus-41434 Theodor-Boveri-Institut für Biowissenschaften OPUS4-3620 Wissenschaftlicher Artikel Hock, Robert; Moormann, Antoon; Fischer, Dagmar; Scheer, Ulrich Absence of somatic histone H1 in oocytes and preblastula embryos of Xenopus laevis Available data on the occurrence and expression of somatic histone HI during oogenesis and early embryogenesis of Xenopus laevis are contradictory. In particular the reported presence of a large storage pool of histone HIA in oocytes is difficult to reconcile with the high transcriptional activity of all gene classes in this specific cell type. In the present study we have used polyclonal antibodies raised against somatic Xenopus histone HI (HIA and HIA/B) for combined immunoblotting experiments to quantitate HI pools and immunolocalization studies to visualize chromosome- bound HI. Both approaches failed to detect soluble or chromosomal histone HI in vitellogenic oocytes, eggs, and cleavage-stage embryos up to early blastula. In addition, chromatin assembled in Xenopus egg extract was also negative for histone HI as revealed by immunofluorescence microscopy. Lampbrush chromosomes not only lacked histone HI but also the previously identified histone HI-like B4 protein (Smith et al., 1988, Genes Dev. 2,1284-1295). In contrast, chromosomes of eggs and early embryos fluoresced brightly with anti-B4 antibodies. Our results lend further support to the view that histone HI expression is developmentally regulated during Xenopus oogenesis and embryogenesis similar to what is known from other species. 1993 urn:nbn:de:bvb:20-opus-41350 Theodor-Boveri-Institut für Biowissenschaften OPUS4-3129 Wissenschaftlicher Artikel Bell, Peter; Dabauvalle, Marie-Christine; Scheer, Ulrich In vitro assembly of prenucleolar bodies in Xenopus egg extract Nuclei assembled in Xenopus egg extract from purified DNA or chromatin resemble their natural counterparts in a number of structural and functional features. However, the most obvious structural element of normal interphase nuclei, the nucleolus, is absent from the in vitro reconstituted nuclei. By EM, cytological silver staining, and immunofluorescence microscopy employing antibodies directed against various nucleolar components we show that nuclei assembled in vitro contain numerous distinct aggregates that resemble prenucleolar bodies (PNBs) by several criteria. Formation of these PNB-like structures requires pore complex-mediated nuclear transport of proteins but is independent of the genetic content of the in vitro nuclei as well as transcriptional and translational events. Our data indicate that nuclei assembled in vitro are capable of initiating early steps of nucleologenesis but that the resulting PNBs are unable to fuse with each other, probably due to the absence of a functional nucleolus organizer. With appropriate modifications, this experimental system should be useful to define and analyze conditions promoting the site-specific assembly of PNBs into a coherent nucleolar body. 1992 urn:nbn:de:bvb:20-opus-34233 Theodor-Boveri-Institut für Biowissenschaften