Dokument-ID Dokumenttyp Verfasser/Autoren Herausgeber Haupttitel Abstract Auflage Verlagsort Verlag Erscheinungsjahr Seitenzahl Schriftenreihe Titel Schriftenreihe Bandzahl ISBN Quelle der Hochschulschrift Konferenzname Quelle:Titel Quelle:Jahrgang Quelle:Heftnummer Quelle:Erste Seite Quelle:Letzte Seite URN DOI Abteilungen OPUS4-11973 Wissenschaftlicher Artikel Proppert, Sven; Wolter, Steve; Holm, Thorge; Klein, Theresa; van de Linde, Sebastian; Sauer, Markus Cubic B-spline calibration for 3D super-resolution measurements using astigmatic imaging In recent years three-dimensional (3D) super-resolution fluorescence imaging by single-molecule localization (localization microscopy) has gained considerable interest because of its simple implementation and high optical resolution. Astigmatic and biplane imaging are experimentally simple methods to engineer a 3D-specific point spread function (PSF), but existing evaluation methods have proven problematic in practical application. Here we introduce the use of cubic B-splines to model the relationship of axial position and PSF width in the above mentioned approaches and compare the performance with existing methods. We show that cubic B-splines are the first method that can combine precision, accuracy and simplicity. 2014 10304-16 Optics Express 22 9 urn:nbn:de:bvb:20-opus-119730 10.1364/OE.22.010304 Theodor-Boveri-Institut für Biowissenschaften