Dokument-ID Dokumenttyp Verfasser/Autoren Herausgeber Haupttitel Abstract Auflage Verlagsort Verlag Erscheinungsjahr Seitenzahl Schriftenreihe Titel Schriftenreihe Bandzahl ISBN Quelle der Hochschulschrift Konferenzname Quelle:Titel Quelle:Jahrgang Quelle:Heftnummer Quelle:Erste Seite Quelle:Letzte Seite URN DOI Abteilungen OPUS4-22845 Wissenschaftlicher Artikel Scheer, Ulrich Boveri's research at the Zoological Station Naples: Rediscovery of his original microscope slides at the University of Würzburg Eric Davidson once wrote about Theodor Boveri: "From his own researches, and perhaps most important, his generalized interpretations, derive the paradigms that underlie modern inquiries into the genomic basis of embryogenesis" (Davidson, 1985). As luck would have it, the "primary data" of Boveri's experimental work, namely the microscope slides prepared by him and his wife Marcella during several stays at the Zoological Station in Naples (1901/02, 1911/12 and 1914), have survived at the University of Wurzburg. More than 600 slides exist and despite their age they are in a surprisingly good condition. The slides are labelled and dated in Boveri's handwriting and thus can be assigned to his published experimental work on sea urchin development. The results allowed Boveri to unravel the role of the cell nucleus and its chromosomes in development and inheritance. Here, I present an overview of the slides in the context of Boveri's work along with photographic images of selected specimens taken from the original slides. It is planned to examine the slides in more detail, take high-resolution focal image series of significant specimens and make them online available. 2018 1-8 Marine Genomics 40 urn:nbn:de:bvb:20-opus-228453 10.1016/j.margen.2018.01.003 Theodor-Boveri-Institut für Biowissenschaften OPUS4-6945 Wissenschaftlicher Artikel Fischer, D.; Weisenberger, D.; Scheer, Ulrich In situ hybridization of DIG-labeled rRNA probes to mouse liver ultrathin sections No abstract available. 1992 urn:nbn:de:bvb:20-opus-69458 Theodor-Boveri-Institut für Biowissenschaften OPUS4-6850 Wissenschaftlicher Artikel Scheer, Ulrich Harold Garnet Callan 1917-1993 Professor Harold Gamet Callan, honorary member of the German Society for Cell Biology, died on the 3rd November 1993, at the age of 76. His name is inseparably connected with lampbrush chromosomes, the most spectacular and aesthetically ailuring form of chromosomes, which occupied the major part of his scientific career. " Mick" Callan's pioneering studies led to fruitful new concepts, served as a building block for many subsequent studies by others, and contributed enormously to our current understanding of chromosome organization and activity ... 1994 urn:nbn:de:bvb:20-opus-80789 Theodor-Boveri-Institut für Biowissenschaften OPUS4-6849 Wissenschaftlicher Artikel Scheer, Ulrich Das Chromatin : seine Struktur und Funktion no abstract available 1986 urn:nbn:de:bvb:20-opus-80790 Theodor-Boveri-Institut für Biowissenschaften OPUS4-3682 Wissenschaftlicher Artikel Scheer, Ulrich A novel type of chromatin organization in lampbrush chromosomes of Pleurodeles waltlii: visualization of clusters of tandemly repeated, very short transcriptional units A novel chromatin configuration is described in lampbrush chromosomes of Pleurodeles waltlii oocytes which is different from transcriptionally inactive chromatin as weil as from the various forms of transcribed chromatin hitherto described. This novel type of chromatin is not arranged in Christmas tree-Iike configurations of densely packed lateral ribonucleoprotein (RNP) fibriIs but is characterized by a periodic alternating pattern of thick and thin regions which occur in clusters 01 some 10,000 repeats. Each thickened unit with an average length of 45 nm contains two c10sely spaced particles, the putative RNA polymerases, and each thickened unit is separated from the next one by a beaded chromatin spacer with a length of about 80 nm. This chromatin spacer contains on average two particles of approximately 14 nm in diameter, assumed to be nucleosomes. The thickened regions are interpreted to represent short transcriptional units containing approximately 130 base pairs of DNA which are separated from each other by nontranscribed spacers of 240-400 base pairs of DNA. The possibility is discussed that these transcriptional units represent 5S rRNA or tRNA genes. 1982 urn:nbn:de:bvb:20-opus-41087 Theodor-Boveri-Institut für Biowissenschaften OPUS4-3658 Wissenschaftlicher Artikel Benavente, Ricardo; Scheer, Ulrich; Chaly, Nathalie Nucleocytoplasmic sorting of macromolecules following mitosis: fate of nuclear constituents after inhibition of pore complex function PtK2 cells in which pore complex-mediated transport is blocked by microinjection early in mitosis of a monoclonal antibody (specific for an Mr 68000 pore complex glycoprotein) or of wheat germ agglutinin (WGA) complete cytokinesis. However, their nuclei remain stably arrested in a telophase-like organization characterized by highly condensed chromatin and the absence of nucleoli, indicating a requirement for pore-mediated transport for the reassembly of interphase nuclei. We have now examined this requirement more closely by monitoring the behavior of individual nuclear macromolecules in microinjected cells using immunofluorescence microscopy and have investigated the effect of microinjecting the antibody or WGA on cellular ultrastructure. The absence of nuclear transport did not affect the sequestration into daughter nuclei of components such as DNA, DNA topoisomerase I and the nucleolar protein fibrillarin that are carried through mitosis on chromosomes. On the other hand, lamins, snRNAs and the p68 pore complex glycoprotein, all cytoplasmic during mitosis, remained largely cytoplasmic in the telophase-arrested cells. Electron microscopy showed the nuclei to be surrounded by a doublelayered membrane with some inserted pore complexes. In addition, however, a variety of membranous structures with associated pore complexes was regularly noted in the cytoplasm, suggesting that chromatin may not be essential for the postmitotic formation of pore complexes. We propose that cellular compartmentalization at telophase is a two-step process. First, a nuclear envelope tightly encloses the condensed chromosomes, excluding non-selectively all macromolecules not associated with the chromosomes. Interphase nuclear organization is then progressively restored by selective pore complex-mediated uptake of nuclear proteins from the cytoplasm. 1989 urn:nbn:de:bvb:20-opus-40777 Theodor-Boveri-Institut für Biowissenschaften OPUS4-3637 Wissenschaftlicher Artikel Knecht, Sigrid; Scheer, Ulrich Die Liste der Vogelarten von S. Miguel (Azoren) des Gaspar Fructuoso (gestorben 1591) No abstract available 1972 urn:nbn:de:bvb:20-opus-41402 Theodor-Boveri-Institut für Biowissenschaften OPUS4-3635 Wissenschaftlicher Artikel Franke, Werner W.; Jarasch, Ernst-Dieter; Herth, Werner; Scheer, Ulrich; Zerban, Heide Cytology : general and molecular cytology The present review discusses some general aspects of membrane structure and problems of membrane isolation and membrane biochemistry, with particular focus on the endoplasmic reticulum. 1975 urn:nbn:de:bvb:20-opus-41458 Theodor-Boveri-Institut für Biowissenschaften OPUS4-3634 Wissenschaftlicher Artikel Spring, Herbert; Krohne, Georg; Franke, Werner W.; Scheer, Ulrich; Trendelenburg, Michael F. Homogeneity and heterogeneity of sizes of transcriptional units and spacer regions in nucleolar genes of Acetabularia The arrangement of genes of precursor molecules for ribosomal RNA (pre-rRNA) in primary nuclei from two green algae species, Acetabularia mediterranea and A. major, has been analyzed in an electron microscope study. The pattern of transcriptional units in individual strands of nucleolar chromatin was investigated using spread and positively stained preparations. The rDNA pattern is not uniform but differs in different strands. The predominant type of nucleolar chromatin exhibits a high degree of homogeneity in the sequence of matrix units (intercepts covered with fibrilst hat contain the pre-rRNA) and fibril-free spacer intercepts. Substantial differences, however, are observed between the patterns in different strands. In addition, there is evidence in some strands for intraaxial heterogeneity of both spacer and matrix units. The following major types can be distinguished: type la, ca. 2 micrometer long matrix units, extremely short spacer intercepts in A. mediterranea (ca. 1 micrometer long ones in A. major), completely homogeneous distribution; type Ib, as type la but with intercalated, isolated, significantly shorter and/or longer matrix units; type lIa, matrix unit sizes as in type la, but much longer spacer intercepts, high degree of homogeneity; type Ill, largely heterogeneous arrangements of matrix and spacer units of varying sizes. The matrix unit data are compared with the sizes of pre-rRNA as determined by polyacrylamide gelelectrophoresis under denaturing and non-denaturing conditions. The findings are discussed in relation to recent observations in amphibia and insects and with respect to current concepts of the species-specificity of rDNA arrangements. 1976 urn:nbn:de:bvb:20-opus-41398 Theodor-Boveri-Institut für Biowissenschaften OPUS4-3633 Wissenschaftlicher Artikel Weber, Klaus; Osborn, Mary; Franke, Werner W.; Seib, Erinita; Scheer, Ulrich; Herth, Werner Identification of microtubular structures in diverse plant and animal cells by immunological cross-reaction revealed in immunofluorescence microscopy using antibodies against tubulin from porcine brain Antibody against tubulin from porcine brain was used to evaluate the immunological cross reactivity of tubulin from a variety of animal and plant cells. Indirect immunofluorescence microscopy revealed microtubule-containing structures including cytoplasmic microtubules, spindle microtubules, cilia and fIagella. Thus tubulin from diverse species of both mammals and plants show immunological cross-reactivity with tubulin from porcine brain. Results obtained by immunofluorescence microscopy are whenever possible compared with previously known ultrastructural results obtained by electron microscopy. 1977 urn:nbn:de:bvb:20-opus-41383 Theodor-Boveri-Institut für Biowissenschaften