Dokument-ID Dokumenttyp Verfasser/Autoren Herausgeber Haupttitel Abstract Auflage Verlagsort Verlag Erscheinungsjahr Seitenzahl Schriftenreihe Titel Schriftenreihe Bandzahl ISBN Quelle der Hochschulschrift Konferenzname Quelle:Titel Quelle:Jahrgang Quelle:Heftnummer Quelle:Erste Seite Quelle:Letzte Seite URN DOI Abteilungen OPUS4-13408 Wissenschaftlicher Artikel Endesfelder, Ulrike; Malkusch, Sebastian; Flottmann, Benjamin; Mondry, Justine; Liguzinski, Piotr; Verveer, Peter J.; Heilemann, Mike Chemically Induced Photoswitching of Fluorescent Probes - A General Concept for Super-Resolution Microscopy We review fluorescent probes that can be photoswitched or photoactivated and are suited for single-molecule localization based super-resolution microscopy. We exploit the underlying photochemical mechanisms that allow photoswitching of many synthetic organic fluorophores in the presence of reducing agents, and study the impact of these on the photoswitching properties of various photoactivatable or photoconvertible fluorescent proteins. We have identified mEos2 as a fluorescent protein that exhibits reversible photoswitching under various imaging buffer conditions and present strategies to characterize reversible photoswitching. Finally, we discuss opportunities to combine fluorescent proteins with organic fluorophores for dual-color photoswitching microscopy. 2011 3106-3118 Molecules 16 4 urn:nbn:de:bvb:20-opus-134080 10.3390/molecules16043106 Theodor-Boveri-Institut für Biowissenschaften OPUS4-6365 Wissenschaftlicher Artikel Endesfelder, Ulrike; Malkusch, Sebastian; Flottmann, Benjamin; Mondry, Justine; Liguzinski, Piotr; Verveer, Peter J.; Heilemann, Mike Chemically Induced Photoswitching of Fluorescent Probes - A General Concept for Super-Resolution Microscopy We review fluorescent probes that can be photoswitched or photoactivated and are suited for single-molecule localization based super-resolution microscopy. We exploit the underlying photochemical mechanisms that allow photoswitching of many synthetic organic fluorophores in the presence of reducing agents, and study the impact of these on the photoswitching properties of various photoactivatable or photoconvertible fluorescent proteins. We have identified mEos2 as a fluorescent protein that exhibits reversible photoswitching under various imaging buffer conditions and present strategies to characterize reversible photoswitching. Finally, we discuss opportunities to combine fluorescent proteins with organic fluorophores for dual-color photoswitching microscopy. 2011 urn:nbn:de:bvb:20-opus-74896 Theodor-Boveri-Institut für Biowissenschaften