Dokument-ID Dokumenttyp Verfasser/Autoren Herausgeber Haupttitel Abstract Auflage Verlagsort Verlag Erscheinungsjahr Seitenzahl Schriftenreihe Titel Schriftenreihe Bandzahl ISBN Quelle der Hochschulschrift Konferenzname Quelle:Titel Quelle:Jahrgang Quelle:Heftnummer Quelle:Erste Seite Quelle:Letzte Seite URN DOI Abteilungen OPUS4-26567 Wissenschaftlicher Artikel Adolfi, Mateus C.; Du, Kang; Kneitz, Susanne; Cabau, Cédric; Zahm, Margot; Klopp, Christophe; Feron, Romain; Paixão, Rômulo V.; Varela, Eduardo S.; de Almeida, Fernanda L.; de Oliveira, Marcos A.; Nóbrega, Rafael H.; Lopez-Roques, Céline; Iampietro, Carole; Lluch, Jérôme; Kloas, Werner; Wuertz, Sven; Schaefer, Fabian; Stöck, Matthias; Guiguen, Yann; Schartl, Manfred A duplicated copy of id2b is an unusual sex-determining candidate gene on the Y chromosome of arapaima (Arapaima gigas) Arapaima gigas is one of the largest freshwater fish species of high ecological and economic importance. Overfishing and habitat destruction are severe threats to the remaining wild populations. By incorporating a chromosomal Hi-C contact map, we improved the arapaima genome assembly to chromosome-level, revealing an unexpected high degree of chromosome rearrangements during evolution of the bonytongues (Osteoglossiformes). Combining this new assembly with pool-sequencing of male and female genomes, we identified id2bbY, a duplicated copy of the inhibitor of DNA binding 2b (id2b) gene on the Y chromosome as candidate male sex-determining gene. A PCR-test for id2bbY was developed, demonstrating that this gene is a reliable male-specific marker for genotyping. Expression analyses showed that this gene is expressed in juvenile male gonads. Its paralog, id2ba, exhibits a male-biased expression in immature gonads. Transcriptome analyses and protein structure predictions confirm id2bbY as a prime candidate for the master sex-determiner. Acting through the TGF beta signaling pathway, id2bbY from arapaima would provide the first evidence for a link of this family of transcriptional regulators to sex determination. Our study broadens our current understanding about the evolution of sex determination genetic networks and provide a tool for improving arapaima aquaculture for commercial and conservation purposes. 2021 Scientific Reports 11 1 urn:nbn:de:bvb:20-opus-265672 10.1038/s41598-021-01066-z Theodor-Boveri-Institut für Biowissenschaften OPUS4-20133 Wissenschaftlicher Artikel Du, Kang; Wuertz, Sven; Adolfi, Mateus; Kneitz, Susanne; Stöck, Matthias; Oliveira, Marcos; Nóbrega, Rafael; Ormanns, Jenny; Kloas, Werner; Feron, Romain; Klopp, Christophe; Parrinello, Hugues; Journot, Laurent; He, Shunping; Postlethwait, John; Meyer, Axel; Guiguen, Yann; Schartl, Manfred The genome of the arapaima (Arapaima gigas) provides insights into gigantism, fast growth and chromosomal sex determination system We have sequenced the genome of the largest freshwater fish species of the world, the arapaima. Analysis of gene family dynamics and signatures of positive selection identified genes involved in the specific adaptations and unique features of this iconic species, in particular it's large size and fast growth. Genome sequences from both sexes combined with RAD-tag analyses from other males and females led to the isolation of male-specific scaffolds and supports an XY sex determination system in arapaima. Whole transcriptome sequencing showed that the product of the gland-like secretory organ on the head surface of males and females may not only provide nutritional fluid for sex-unbiased parental care, but that the organ itself has a more specific function in males, which engage more in parental care. 2019 5293 Scientific Reports 9 urn:nbn:de:bvb:20-opus-201333 10.1038/s41598-019-41457-x Theodor-Boveri-Institut für Biowissenschaften