Dokument-ID Dokumenttyp Verfasser/Autoren Herausgeber Haupttitel Abstract Auflage Verlagsort Verlag Erscheinungsjahr Seitenzahl Schriftenreihe Titel Schriftenreihe Bandzahl ISBN Quelle der Hochschulschrift Konferenzname Quelle:Titel Quelle:Jahrgang Quelle:Heftnummer Quelle:Erste Seite Quelle:Letzte Seite URN DOI Abteilungen OPUS4-12068 Wissenschaftlicher Artikel Dandekar, Thomas; Fieselmann, Astrid; Fischer, Eva; Popp, Jasmin; Hensel, Michael; Noster, Janina Salmonella—how a metabolic generalist adopts an intracellular lifestyle during infection The human-pathogenic bacterium Salmonella enterica adjusts and adapts to different environments while attempting colonization. In the course of infection nutrient availabilities change drastically. New techniques, "-omics" data and subsequent integration by systems biology improve our understanding of these changes. We review changes in metabolism focusing on amino acid and carbohydrate metabolism. Furthermore, the adaptation process is associated with the activation of genes of the Salmonella pathogenicity islands (SPIs). Anti-infective strategies have to take these insights into account and include metabolic and other strategies. Salmonella infections will remain a challenge for infection biology. 2014 Frontiers in Cellular and Infection Microbiology 4 191 urn:nbn:de:bvb:20-opus-120686 10.3389/fcimb.2014.00191 Theodor-Boveri-Institut für Biowissenschaften OPUS4-12313 Wissenschaftlicher Artikel Dandekar, Thomas; Fieselmann, Astrid; Popp, Jasmin; Hensel, Michael Salmonella enterica: a surprisingly well-adapted intracellular lifestyle The infectious intracellular lifestyle of Salmonella enterica relies on the adaptation to nutritional conditions within the Salmonella-containing vacuole (SCV) in host cells. We summarize latest results on metabolic requirements for Salmonella during infection. This includes intracellular phenotypes of mutant strains based on metabolic modeling and experimental tests, isotopolog profiling using (13)C-compounds in intracellular Salmonella, and complementation of metabolic defects for attenuated mutant strains towards a comprehensive understanding of the metabolic requirements of the intracellular lifestyle of Salmonella. Helpful for this are also genomic comparisons. We outline further recent studies and which analyses of intracellular phenotypes and improved metabolic simulations were done and comment on technical required steps as well as progress involved in the iterative refinement of metabolic flux models, analyses of mutant phenotypes, and isotopolog analyses. Salmonella lifestyle is well-adapted to the SCV and its specific metabolic requirements. Salmonella metabolism adapts rapidly to SCV conditions, the metabolic generalist Salmonella is quite successful in host infection. 2012 Frontiers in Microbiology urn:nbn:de:bvb:20-opus-123135 Theodor-Boveri-Institut für Biowissenschaften OPUS4-14902 Wissenschaftlicher Artikel Dandekar, Thomas; Fieselmann, Astrid; Fischer, Eva; Popp, Jasmin; Hensel, Michael; Noster, Janina Salmonella - how a metabolic generalist adopts an intracellular lifestyle during infection The human-pathogenic bacterium Salmonella enterica adjusts and adapts to different environments while attempting colonization. In the course of infection nutrient availabilities change drastically. New techniques, "-omics" data and subsequent integration by systems biology improve our understanding of these changes. We review changes in metabolism focusing on amino acid and carbohydrate metabolism. Furthermore, the adaptation process is associated with the activation of genes of the Salmonella pathogenicity islands (SPIs). Anti-infective strategies have to take these insights into account and include metabolic and other strategies. Salmonella infections will remain a challenge for infection biology. 2015 Frontiers in Cellular and Infection Microbiology 4 191 urn:nbn:de:bvb:20-opus-149029 10.3389/fcimb.2014.00191 Theodor-Boveri-Institut für Biowissenschaften