Dokument-ID Dokumenttyp Verfasser/Autoren Herausgeber Haupttitel Abstract Auflage Verlagsort Verlag Erscheinungsjahr Seitenzahl Schriftenreihe Titel Schriftenreihe Bandzahl ISBN Quelle der Hochschulschrift Konferenzname Quelle:Titel Quelle:Jahrgang Quelle:Heftnummer Quelle:Erste Seite Quelle:Letzte Seite URN DOI Abteilungen OPUS4-3613 Wissenschaftlicher Artikel Scheer, Ulrich; Messner, Karin; Hazan, Rachel; Raska, Ivan; Hansmann, Paul; Falk, Heinz; Spiess, Eberhard; Franke, Werner W. High sensitivity immunolocalization of double and single-stranded DNA by a monoclonal antibody A monoclonal antibody (AK 30-10) is described which specifically reacts with DNA both in double and single-stranded forms but not with other molecules and structures, including deoxyribonucleotides and RNAs. When used in immunocytochemical experiments on tissue sections and permeabilized cultured cells, this antibody detects DNA-containing structures, even when the DNA is present in very small amounts. Examples of high resolution detection include the DNA present in amplified extrachromosomal nucleoli, chromomeres of lampbrush chromosomes, mitochondria, chloroplasts and mycoplasmal particles. In immunoelectron microscopy using the immunogold technique, the DNA was localized in distinct substructures such as the "fibrillar centers" of nucleoli and certain stromal centers in chloroplasts. The antibody also reacts with DNA of chromatin of living cells, as shown by microinjection into cultured mitotic cells and into nuclei of amphibian oocytes. The potential value and the limitations of immunocytochemical DNA detection are discussed. 1987 urn:nbn:de:bvb:20-opus-41063 Theodor-Boveri-Institut für Biowissenschaften OPUS4-3638 Review Reimer, Georg; Raska, Ivan; Tan, Eng M.; Scheer, Ulrich Human autoantibodies: probes for nucleolus structure and function No abstract available 1987 urn:nbn:de:bvb:20-opus-41410 Theodor-Boveri-Institut für Biowissenschaften OPUS4-3209 Wissenschaftlicher Artikel Reimer, Georg; Raska, Ivan; Scheer, Ulrich; Tan, Eng M. Immunolocalization of 7-2-ribonucleoprotein in the granular component of the nucleolus Certain autoimmune sera contain antibodies against a nucleolar ribonucleoprotein particle associated with 7-2-RNA (R. Reddy et al. (1983) J. Bioi. Chem . 258, 1383; C. Hashimoto and J. A. Steitz (1983) J. Bioi. Chem. 258, 1379). In this study, we showed by immunofluorescence microscopy that antibodies reactive with 7-2-ribonucleoprotein immunolocalized in the granular regions of actinomycin D and 5,6-dichloro-I-j3-D-ribofuranosylbenzimidazole (DRB)-segregated nucleoli from Vero cells. By electron microscopic immunocytochemistry, antigen-antibody complexes were located in the granular component of transcriptionally active nucleoli from rat liver hepatocytes and HeLa cells. Anti-7- 2-RNP antibodies from two autoimmune sera immunoprecipitated a major protein of Mr 40,000 from e5S] methionine-Iabeled HeLa cell extract. The immunolocalization data suggest that 7-2-ribonucleoprotein may be involved in stages of ribosome biogenesis which take place in the granular component of the nucleolus, i.e., assembly, maturation, and/or transport of preribosomes 1988 urn:nbn:de:bvb:20-opus-33890 Theodor-Boveri-Institut für Biowissenschaften