Dokument-ID Dokumenttyp Verfasser/Autoren Herausgeber Haupttitel Abstract Auflage Verlagsort Verlag Erscheinungsjahr Seitenzahl Schriftenreihe Titel Schriftenreihe Bandzahl ISBN Quelle der Hochschulschrift Konferenzname Quelle:Titel Quelle:Jahrgang Quelle:Heftnummer Quelle:Erste Seite Quelle:Letzte Seite URN DOI Abteilungen OPUS4-28725 Wissenschaftlicher Artikel Czysch, Christian; Medina-Montano, Carolina; Zhong, Zifu; Fuchs, Alexander; Stickdorn, Judith; Winterwerber, Pia; Schmitt, Sascha; Deswarte, Kim; Raabe, Marco; Scherger, Maximilian; Combes, Francis; De Vrieze, Jana; Kasmi, Sabah; Sandners, Niek N.; Lienenklaus, Stefan; Koynov, Kaloian; Räder, Hans-Joachim; Lambrecht, Bart N.; David, Sunil A.; Bros, Matthias; Schild, Hansjörg; Grabbe, Stephan; De Geest, Bruno G.; Nuhn, Lutz Transient Lymph Node Immune Activation by Hydrolysable Polycarbonate Nanogels The development of controlled biodegradable materials is of fundamental importance in immunodrug delivery to spatiotemporally controlled immune stimulation but avoid systemic inflammatory side effects. Based on this, polycarbonate nanogels are developed as degradable micellar carriers for transient immunoactivation of lymph nodes. An imidazoquinoline-type TLR7/8 agonist is covalently conjugated via reactive ester chemistry to these nanocarriers. The nanogels not only provide access to complete disintegration by the hydrolysable polymer backbone, but also demonstrate a gradual disintegration within several days at physiological conditions (PBS, pH 6.4-7.4, 37 °C). These intrinsic properties limit the lifetime of the carriers but their payload can still be successfully leveraged for immunological studies in vitro on primary immune cells as well as in vivo. For the latter, a spatiotemporal control of immune cell activation in the draining lymph node is found after subcutaneous injection. Overall, these features render polycarbonate nanogels a promising delivery system for transient activation of the immune system in lymph nodes and may consequently become very attractive for further development toward vaccination or cancer immunotherapy. Due to the intrinsic biodegradability combined with the high chemical control during the manufacturing process, these polycarbonate-based nanogels may also be of great importance for clinical translation. 2022 Advanced Functional Materials 32 35 urn:nbn:de:bvb:20-opus-287255 10.1002/adfm.202203490 Fakultät für Chemie und Pharmazie