3554
1991
eng
review
1
2010-01-12
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Effect of ciliary neurotrophic factor (CNTF) on motoneuron survival
We have demonstrated that the extensive degeneration of motoneurons in the rat facial nucleus after transection of the facial nerve in newborn rats can be prevented by local ciliary neurotrophic factor (CNTF) administration. CNTF differs distinctly from known neurotrophic molecules such as NGF, BDNF and NT-3 in both its molecular characteristics (CNTF is a cytosolic rather than a secretory molecule) and its broad spectrum of biological activities. CNTF is expressed selectively by Schwann cells and astrocytes of the peripheral and central nervous system, respectively, but not by target tissues of the great variety of CNTF -responsive neurons. CNTF mRNA is not detectable by Northern blot or PCR analysis during embryonic development and immediately after birth. However, during the second post-natal week, a more than 30-fold increase in CNTF mRNA and pro tein occurs in the sciatic nerve. Since the period of low CNTF levels in peripheral nerves coincides with that of high vulnerability of motoneurons (i.e. axonallesion results in degeneration of motoneuron cell bodies), insufficient availability of CNTF may be the reason for the rate of lesioninduced cell death of early post-natal motoneurons. Highly enriched embryonic chick motoneurons in culture are supported at survival rates higher than 60% by CNTF, even in single cell cultures, indicating that CNTF acts directly on motoneurons. In contrast to CNTF, the members of the neurotrophin gene family (NGF, BDNF and NT-3) do not support the survival of motoneurons in culture. However, aFGF and bFGF show distinct survival activities which are additive to those of CNTF, resulting in the survival of virtually all motoneurons cultured in the presence of CNTF and bFGF.
urn:nbn:de:bvb:20-opus-33048
3304
In: Journal of cell science (1991) Suppl. 15, 103-109.
Michael Sendtner
Yoshihiro Arakawa
Kurt A. Stöckli
Georg W. Kreutzberg
Hans Thoenen
eng
uncontrolled
motoneurons
eng
uncontrolled
ciliary neurotrophic factor
eng
uncontrolled
CNTF
eng
uncontrolled
nerve lesion
eng
uncontrolled
rat
eng
uncontrolled
chick
eng
uncontrolled
neurotrophic factor
Medizin und Gesundheit
open_access
Institut für Klinische Neurobiologie
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/3554/Sendtner_effect_ciliary.pdf
3084
1990
eng
article
1
2009-08-25
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Survival effect of ciliary neurotrophic factor (CNTF) on chick embryonic motoneurons in culture: comparison with other neurotrophic factors and cytokines
No abstract available
urn:nbn:de:bvb:20-opus-31718
3171
In: The Journal of Neuroscience (1990) 10, 11, 3507-3515.
Yoshihiro Arakawa
Michael Sendtner
Hans Thoenen
Medizin und Gesundheit
open_access
Institut für Klinische Neurobiologie
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/3084/Sendtner_Survival.pdf