22988
2020
eng
11
article
1
2021-03-09
--
--
Integrative functional genomics decodes herpes simplex virus 1
The predicted 80 open reading frames (ORFs) of herpes simplex virus 1 (HSV-1) have been intensively studied for decades. Here, we unravel the complete viral transcriptome and translatome during lytic infection with base-pair resolution by computational integration of multi-omics data. We identify a total of 201 transcripts and 284 ORFs including all known and 46 novel large ORFs. This includes a so far unknown ORF in the locus deleted in the FDA-approved oncolytic virus Imlygic. Multiple transcript isoforms expressed from individual gene loci explain translation of the vast majority of ORFs as well as N-terminal extensions (NTEs) and truncations. We show that NTEs with non-canonical start codons govern the subcellular protein localization and packaging of key viral regulators and structural proteins. We extend the current nomenclature to include all viral gene products and provide a genome browser that visualizes all the obtained data from whole genome to single-nucleotide resolution. Here, using computational integration of multi-omics data, the authors provide a detailed transcriptome and translatome of herpes simplex virus 1 (HSV-1), including previously unidentified ORFs and N-terminal extensions. The study also provides a HSV-1 genome browser and should be a valuable resource for further research.
Nature Communications
10.1038/s41467-020-15992-5
urn:nbn:de:bvb:20-opus-229884
publish
Nature Communications (2020) 11:2038. https://doi.org/10.1038/s41467-020-15992-5 |
CoG 721016–HERPES
true
true
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Adam W. Whisnant
Christopher S. Jürges
Thomas Hennig
Emanuel Wyler
Bhupesh Prusty
Andrzej J. Rutkowski
Anne L'hernault
Lara Djakovic
Margarete Göbel
Kristina Döring
Jennifer Menegatti
Robin Antrobus
Nicholas J. Matheson
Florian W. H. Künzig
Guido Mastrobuoni
Chris Bielow
Stefan Kempa
Chunguang Liang
Thomas Dandekar
Ralf Zimmer
Markus Landthaler
Friedrich Grässer
Paul J. Lehner
Caroline C. Friedel
Florian Erhard
Lars Dölken
eng
uncontrolled
infected-cell protein
eng
uncontrolled
messenger RNA
eng
uncontrolled
binding protein
eng
uncontrolled
type 1
eng
uncontrolled
identification
eng
uncontrolled
ICP27
eng
uncontrolled
translation
eng
uncontrolled
expression
eng
uncontrolled
sequence
eng
uncontrolled
domain
Biowissenschaften; Biologie
Medizin und Gesundheit
open_access
Institut für Virologie und Immunbiologie
Theodor-Boveri-Institut für Biowissenschaften
OpenAIRE
Förderzeitraum 2020
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/22988/s41467-020-15992-5.pdf
14904
2015
eng
7798
5
article
1
2017-05-22
--
--
The CarO rhodopsin of the fungus Fusarium fujikuroi is a light-driven proton pump that retards spore germination
Rhodopsins are membrane-embedded photoreceptors found in all major taxonomic kingdoms using retinal as their chromophore. They play well-known functions in different biological systems, but their roles in fungi remain unknown. The filamentous fungus Fusarium fujikuroi contains two putative rhodopsins, CarO and OpsA. The gene carO is light-regulated, and the predicted polypeptide contains all conserved residues required for proton pumping. We aimed to elucidate the expression and cellular location of the fungal rhodopsin CarO, its presumed proton-pumping activity and the possible effect of such function on F. fujikuroi growth. In electrophysiology experiments we confirmed that CarO is a green-light driven proton pump. Visualization of fluorescent CarO-YFP expressed in F. fujikuroi under control of its native promoter revealed higher accumulation in spores (conidia) produced by light-exposed mycelia. Germination analyses of conidia from carO\(^{-}\) mutant and carO\(^{+}\) control strains showed a faster development of light-exposed carO-germlings. In conclusion, CarO is an active proton pump, abundant in light-formed conidia, whose activity slows down early hyphal development under light. Interestingly, CarO-related rhodopsins are typically found in plant-associated fungi, where green light dominates the phyllosphere. Our data provide the first reliable clue on a possible biological role of a fungal rhodopsin.
Scientific Reports
10.1038/srep07798
urn:nbn:de:bvb:20-opus-149049
Scientific Reports 5:7798 (2015). DOI: 10.1038/srep07798
CC BY-NC-ND: Creative-Commons-Lizenz: Namensnennung, Nicht kommerziell, Keine Bearbeitungen 4.0 International
Jorge García-Martínez
Michael Brunk
Javier Avalos
Ulrich Terpitz
eng
uncontrolled
microbial rhodopsins
eng
uncontrolled
intracellular pH
eng
uncontrolled
membrane proteins
eng
uncontrolled
mutants
eng
uncontrolled
virulence
eng
uncontrolled
channelrhodopsin-2
eng
uncontrolled
growth
eng
uncontrolled
gene
eng
uncontrolled
expression
eng
uncontrolled
bacteriorhodopsin
Medizin und Gesundheit
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/14904/056_Garcia-Martinez_Scientific_Reports.pdf
14471
2015
eng
e0143057
12
10
article
1
2017-02-21
--
--
Whole body melanoma transcriptome response in medaka
The incidence of malignant melanoma continues to increase each year with poor prognosis for survival in many relapse cases. To reverse this trend, whole body response measures are needed to discover collaborative paths to primary and secondary malignancy. Several species of fish provide excellent melanoma models because fish and human melanocytes both appear in the epidermis, and fish and human pigment cell tumors share conserved gene expression signatures. For the first time, we have examined the whole body transcriptome response to invasive melanoma as a prelude to using transcriptome profiling to screen for drugs in a medaka (Oryzias latipes) model. We generated RNA-seq data from whole body RNA isolates for controls and melanoma fish. After testing for differential expression, 396 genes had significantly different expression (adjusted p-value <0.02) in the whole body transcriptome between melanoma and control fish; 379 of these genes were matched to human orthologs with 233 having annotated human gene symbols and 14 matched genes that contain putative deleterious variants in human melanoma at varying levels of recurrence. A detailed canonical pathway evaluation for significant enrichment showed the top scoring pathway to be antigen presentation but also included the expected melanocyte development and pigmentation signaling pathway. Results revealed a profound down-regulation of genes involved in the immune response, especially the innate immune system. We hypothesize that the developing melanoma actively suppresses the immune system responses of the body in reacting to the invasive malignancy, and that this mal-adaptive response contributes to disease progression, a result that suggests our whole-body transcriptomic approach merits further use. In these findings, we also observed novel genes not yet identified in human melanoma expression studies and uncovered known and new candidate drug targets for further testing in this malignant melanoma medaka model.
PLoS ONE
10.1371/journal.pone.0143057
urn:nbn:de:bvb:20-opus-144714
PLoS ONE 10(12): e0143057 (2015). DOI: 10.1371/journal.pone.0143057
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Manfred Schartl
Yingjia Shen
Katja Maurus
Ron Walter
Chad Tomlinson
Richard K. Wilson
John Postlethwait
Wesley C. Warren
eng
uncontrolled
metastatic melanoma
eng
uncontrolled
expression
eng
uncontrolled
fish
eng
uncontrolled
cancer
eng
uncontrolled
stage III
eng
uncontrolled
melanogenesis
eng
uncontrolled
genome cells
eng
uncontrolled
gene
eng
uncontrolled
contributes
Medizin und Gesundheit
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/14471/082_Schartl_PLoS_ONE.pdf
13444
2012
eng
e45682
9
7
article
1
2016-06-06
--
--
Comparative proteome analysis of Milnesium tardigradum in early embryonic state versus adults in active and anhydrobiotic state
Tardigrades have fascinated researchers for more than 300 years because of their extraordinary capability to undergo cryptobiosis and survive extreme environmental conditions. However, the survival mechanisms of tardigrades are still poorly understood mainly due to the absence of detailed knowledge about the proteome and genome of these organisms. Our study was intended to provide a basis for the functional characterization of expressed proteins in different states of tardigrades. High-throughput, high-accuracy proteomics in combination with a newly developed tardigrade specific protein database resulted in the identification of more than 3000 proteins in three different states: early embryonic state and adult animals in active and anhydrobiotic state. This comprehensive proteome resource includes protein families such as chaperones, antioxidants, ribosomal proteins, cytoskeletal proteins, transporters, protein channels, nutrient reservoirs, and developmental proteins. A comparative analysis of protein families in the different states was performed by calculating the exponentially modified protein abundance index which classifies proteins in major and minor components. This is the first step to analyzing the proteins involved in early embryonic development, and furthermore proteins which might play an important role in the transition into the anhydrobiotic state.
PLoS One
10.1371/journal.pone.0045682
urn:nbn:de:bvb:20-opus-134447
PLoS ONE 7(9): e45682. doi:10.1371/journal.pone.0045682
Elham Schokraie
Uwe Warnken
Agnes Hotz-Wagenblatt
Markus A. Grohme
Steffen Hengherr
Frank Förster
Ralph O. Schill
Marcus Frohme
Thomas Dandekar
Martina Schnölzer
eng
uncontrolled
life-span regulation
eng
uncontrolled
genes
eng
uncontrolled
Yolk protein
eng
uncontrolled
water stress
eng
uncontrolled
expression
eng
uncontrolled
tolerance
eng
uncontrolled
richtersius coronifer
eng
uncontrolled
superoxide-dismutase
eng
uncontrolled
caenorhabditis elegans
eng
uncontrolled
arabidopsis thaliana
eng
uncontrolled
vitellogenin
Datenverarbeitung; Informatik
Biowissenschaften; Biologie
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/13444/Schokraie_PLoSOne.pdf
13349
2012
eng
e1002577
3
8
article
1
2016-05-13
--
--
Physiological Notch Signaling Maintains Bone Homeostasis via RBPjk and Hey Upstream of NFATc1
Notch signaling between neighboring cells controls many cell fate decisions in metazoans both during embryogenesis and in postnatal life. Previously, we uncovered a critical role for physiological Notch signaling in suppressing osteoblast differentiation in vivo. However, the contribution of individual Notch receptors and the downstream signaling mechanism have not been elucidated. Here we report that removal of Notch2, but not Notch1, from the embryonic limb mesenchyme markedly increased trabecular bone mass in adolescent mice. Deletion of the transcription factor RBPjk, a mediator of all canonical Notch signaling, in the mesenchymal progenitors but not the more mature osteoblast-lineage cells, caused a dramatic high-bone-mass phenotype characterized by increased osteoblast numbers, diminished bone marrow mesenchymal progenitor pool, and rapid age-dependent bone loss. Moreover, mice deficient in Hey1 and HeyL, two target genes of Notch-RBPjk signaling, exhibited high bone mass. Interestingly, Hey1 bound to and suppressed the NFATc1 promoter, and RBPjk deletion increased NFATc1 expression in bone. Finally, pharmacological inhibition of NFAT alleviated the high-bone-mass phenotype caused by RBPjk deletion. Thus, Notch-RBPjk signaling functions in part through Hey1-mediated inhibition of NFATc1 to suppress osteoblastogenesis, contributing to bone homeostasis in vivo.
PLoS Genetics
10.1371/journal.pgen.1002577
urn:nbn:de:bvb:20-opus-133490
PLoS Genetics 8(3): e1002577. doi:10.1371/journal.pgen.1002577
Xiaolin Tu
Jianquan Chen
Joohyun Lim
Courtney M. Karner
Seung-Yon Lee
Julia Heisig
Cornelia Wiese
Kameswaran Surendran
Raphael Kopan
Manfred Gessler
Fanxin Long
eng
uncontrolled
expression
eng
uncontrolled
axial skeletal defects
eng
uncontrolled
transcription factor
eng
uncontrolled
alagille syndrome
eng
uncontrolled
osteoblast differentiation
eng
uncontrolled
human jagged1
eng
uncontrolled
aortic-valve
eng
uncontrolled
T cells
eng
uncontrolled
mutations
eng
uncontrolled
mice
Menschliche Anatomie, Zytologie, Histologie
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/13349/Tu_PLoS_2012.pdf
13262
2012
eng
10
12
article
1
2016-04-22
--
--
Genetic and physical interaction of Meis2, Pax3 and Pax7 during dorsal midbrain development
Background: During early stages of brain development, secreted molecules, components of intracellular signaling pathways and transcriptional regulators act in positive and negative feed-back or feed-forward loops at the mid-hindbrain boundary. These genetic interactions are of central importance for the specification and subsequent development of the adjacent mid-and hindbrain. Much less, however, is known about the regulatory relationship and functional interaction of molecules that are expressed in the tectal anlage after tectal fate specification has taken place and tectal development has commenced.
Results: Here, we provide experimental evidence for reciprocal regulation and subsequent cooperation of the paired-type transcription factors Pax3, Pax7 and the TALE-homeodomain protein Meis2 in the tectal anlage. Using in ovo electroporation of the mesencephalic vesicle of chick embryos we show that (i) Pax3 and Pax7 mutually regulate each other's expression in the mesencephalic vesicle, (ii) Meis2 acts downstream of Pax3/7 and requires balanced expression levels of both proteins, and (iii) Meis2 physically interacts with Pax3 and Pax7. These results extend our previous observation that Meis2 cooperates with Otx2 in tectal development to include Pax3 and Pax7 as Meis2 interacting proteins in the tectal anlage.
Conclusion: The results described here suggest a model in which interdependent regulatory loops involving Pax3 and Pax7 in the dorsal mesencephalic vesicle modulate Meis2 expression. Physical interaction with Meis2 may then confer tectal specificity to a wide range of otherwise broadly expressed transcriptional regulators, including Otx2, Pax3 and Pax7.
BMC Developmental Biology
10.1186/1471-213X-12-10
urn:nbn:de:bvb:20-opus-132626
BMC Developmental Biology 2012, 12:10. doi:10.1186/1471-213X-12-10
Zsuzsa Agoston
Naixin Li
Anja Haslinger
Andrea Wizenmann
Dorothea Schulte
eng
uncontrolled
dosage
eng
uncontrolled
quali-chick chimeras
eng
uncontrolled
drosophila embryo
eng
uncontrolled
neural crest
eng
uncontrolled
transcription activation
eng
uncontrolled
hindbrain boundary
eng
uncontrolled
isthmic oragnizer
eng
uncontrolled
sonic hedghog
eng
uncontrolled
expression
eng
uncontrolled
induction
Einzelne physiologische Systeme bei Tieren
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/13262/Agoston_BMC_2012.pdf
13063
2012
eng
e1002768
7
8
article
1
2016-03-24
--
--
Three Dopamine Pathways Induce Aversive Odor Memories with Different Stability
Animals acquire predictive values of sensory stimuli through reinforcement. In the brain of Drosophila melanogaster, activation of two types of dopamine neurons in the PAM and PPL1 clusters has been shown to induce aversive odor memory. Here, we identified the third cell type and characterized aversive memories induced by these dopamine neurons. These three dopamine pathways all project to the mushroom body but terminate in the spatially segregated subdomains. To understand the functional difference of these dopamine pathways in electric shock reinforcement, we blocked each one of them during memory acquisition. We found that all three pathways partially contribute to electric shock memory. Notably, the memories mediated by these neurons differed in temporal stability. Furthermore, combinatorial activation of two of these pathways revealed significant interaction of individual memory components rather than their simple summation. These results cast light on a cellular mechanism by which a noxious event induces different dopamine signals to a single brain structure to synthesize an aversive memory.
PLoS Genetics
10.1371/journal.pgen.1002768
urn:nbn:de:bvb:20-opus-130631
PLoS Genetics 8(7): e1002768. doi:10.1371/journal.pgen.1002768
Yoshinori Aso
Andrea Herb
Maite Ogueta
Igor Siwanowicz
Thomas Templier
Anja B. Friedrich
Kei Ito
Henrike Scholz
Hiromu Tanimoto
eng
uncontrolled
dynamics
eng
uncontrolled
serotonin
eng
uncontrolled
expression
eng
uncontrolled
melanogaster
eng
uncontrolled
neurons form
eng
uncontrolled
olfactory memory
eng
uncontrolled
long-term-memory
eng
uncontrolled
drosophila mushroom body
eng
uncontrolled
sensitization
eng
uncontrolled
localization
Genetik und Evolution
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/13063/journal.pgen.1002768.pdf
11628
2014
eng
e1004089
5
10
article
1
2015-07-20
--
--
A Nasal Epithelial Receptor for Staphylococcus aureus WTA Governs Adhesion to Epithelial Cells and Modulates Nasal Colonization
Nasal colonization is a major risk factor for S. aureus infections. The mechanisms responsible for colonization are still not well understood and involve several factors on the host and the bacterial side. One key factor is the cell wall teichoic acid (WTA) of S. aureus, which governs direct interactions with nasal epithelial surfaces. We report here the first receptor for the cell wall glycopolymer WTA on nasal epithelial cells. In several assay systems this type F-scavenger receptor, termed SREC-I, bound WTA in a charge dependent manner and mediated adhesion to nasal epithelial cells in vitro. The impact of WTA and SREC-I interaction on epithelial adhesion was especially pronounced under shear stress, which resembles the conditions found in the nasal cavity. Most importantly, we demonstrate here a key role of the WTA-receptor interaction in a cotton rat model of nasal colonization. When we inhibited WTA mediated adhesion with a SREC-I antibody, nasal colonization in the animal model was strongly reduced at the early onset of colonization. More importantly, colonization stayed low over an extended period of 6 days. Therefore we propose targeting of this glycopolymer-receptor interaction as a novel strategy to prevent or control S. aureus nasal colonization.
PLOS PATHOGENS
10.1371/journal.ppat.1004089
1553-7374
24788600
urn:nbn:de:bvb:20-opus-116280
PLoS Pathogens 10(5): e1004089. doi:10.1371/journal.ppat.1004089
Stefanie Baur
Maren Rautenberg
Manuela Faulstich
Timo Grau
Yannik Severin
Clemens Unger
Wolfgang H. Hoffmann
Thomas Rudel
Ingo B. Autenrieth
Christopher Weidenmaier
eng
uncontrolled
SREC-I
eng
uncontrolled
clumping factor-B
eng
uncontrolled
scavender receptor
eng
uncontrolled
teichoic acids
eng
uncontrolled
surface proteins
eng
uncontrolled
cotton rats
eng
uncontrolled
carriage
eng
uncontrolled
determinant
eng
uncontrolled
infections
eng
uncontrolled
expression
Biowissenschaften; Biologie
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/11628/082_Baur_Plos_Pathogens.pdf
11542
2014
eng
e01802-14
5
5
article
1
2015-07-07
--
--
Septins Arrange F-Actin-Containing Fibers on the Chlamydia trachomatis Inclusion and Are Required for Normal Release of the Inclusion by Extrusion
Chlamydia trachomatis is an obligate intracellular human pathogen that grows inside a membranous, cytosolic vacuole termed an inclusion. Septins are a group of 13 GTP-binding proteins that assemble into oligomeric complexes and that can form higher-order filaments. We report here that the septins SEPT2, -9, -11, and probably -7 form fibrillar structures around the chlamydial inclusion. Colocalization studies suggest that these septins combine with F actin into fibers that encase the inclusion. Targeting the expression of individual septins by RNA interference (RNAi) prevented the formation of septin fibers as well as the recruitment of actin to the inclusion. At the end of the developmental cycle of C. trachomatis, newly formed, infectious elementary bodies are released, and this release occurs at least in part through the organized extrusion of intact inclusions. RNAi against SEPT9 or against the combination of SEPT2/7/9 substantially reduced the number of extrusions from a culture of infected HeLa cells. The data suggest that a higher-order structure of four septins is involved in the recruitment or stabilization of the actin coat around the chlamydial inclusion and that this actin recruitment by septins is instrumental for the coordinated egress of C. trachomatis from human cells. The organization of F actin around parasite-containing vacuoles may be a broader response mechanism of mammalian cells to the infection by intracellular, vacuole-dwelling pathogens. IMPORTANCE Chlamydia trachomatis is a frequent bacterial pathogen throughout the world, causing mostly eye and genital infections. C. trachomatis can develop only inside host cells; it multiplies inside a membranous vacuole in the cytosol, termed an inclusion. The inclusion is covered by cytoskeletal "coats" or "cages," whose organization and function are poorly understood. We here report that a relatively little-characterized group of proteins, septins, is required to organize actin fibers on the inclusion and probably through actin the release of the inclusion. Septins are a group of GTP-binding proteins that can organize into heteromeric complexes and then into large filaments. Septins have previously been found to be involved in the interaction of the cell with bacteria in the cytosol. Our observation that they also organize a reaction to bacteria living in vacuoles suggests that they have a function in the recognition of foreign compartments by a parasitized human cell.
MBIO
10.1128/mBio.01802-14
2150-7511
25293760
urn:nbn:de:bvb:20-opus-115421
mBio 5(5):e01802-14. doi:10.1128/mBio.01802-14
Larisa Volceanov
Katharina Herbst
Martin Biniossek
Oliver Schilling
Dirk Haller
Thilo Nölke
Prema Subbarayal
Thomas Rudel
Barbara Zieger
Georg Häcker
eng
uncontrolled
mammalian septins
eng
uncontrolled
host-cells
eng
uncontrolled
binding
eng
uncontrolled
proteins
eng
uncontrolled
organization
eng
uncontrolled
cytoskeleton
eng
uncontrolled
cytokinesis
eng
uncontrolled
mechanisms
eng
uncontrolled
expression
eng
uncontrolled
protease
Biowissenschaften; Biologie
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/11542/047_Volceanov_MBIO.pdf
11540
2014
eng
e105732
9
9
article
1
2015-07-07
--
--
Inhibition of the SR Protein-Phosphorylating CLK Kinases of Plasmodium falciparum Impairs Blood Stage Replication and Malaria Transmission
Cyclin-dependent kinase-like kinases (CLKs) are dual specificity protein kinases that phosphorylate Serine/Arginine-rich (SR) proteins involved in pre-mRNA processing. Four CLKs, termed PfCLK-1-4, can be identified in the human malaria parasite Plasmodium falciparum, which show homology with the yeast SR protein kinase Sky1p. The four PfCLKs are present in the nucleus and cytoplasm of the asexual blood stages and of gametocytes, sexual precursor cells crucial for malaria parasite transmission from humans to mosquitoes. We identified three plasmodial SR proteins, PfSRSF12, PfSFRS4 and PfSF-1, which are predominantly present in the nucleus of blood stage trophozoites, PfSRSF12 and PfSF-1 are further detectable in the nucleus of gametocytes. We found that recombinantly expressed SR proteins comprising the Arginine/Serine (RS)-rich domains were phosphorylated by the four PfCLKs in in vitro kinase assays, while a recombinant PfSF-1 peptide lacking the RS-rich domain was not phosphorylated. Since it was hitherto not possible to knock-out the pfclk genes by conventional gene disruption, we aimed at chemical knock-outs for phenotype analysis. We identified five human CLK inhibitors, belonging to the oxo-beta-carbolines and aminopyrimidines, as well as the antiseptic chlorhexidine as PfCLK-targeting compounds. The six inhibitors block P. falciparum blood stage replication in the low micromolar to nanomolar range by preventing the trophozoite-to-schizont transformation. In addition, the inhibitors impair gametocyte maturation and gametogenesis in in vitro assays. The combined data show that the four PfCLKs are involved in phosphorylation of SR proteins with essential functions for the blood and sexual stages of the malaria parasite, thus pointing to the kinases as promising targets for antimalarial and transmission blocking drugs.
PLOS ONE
10.1371/journal.pone.0105732
1932-6203
25188378
urn:nbn:de:bvb:20-opus-115405
PLoS ONE 9(9): e105732. doi:10.1371/journal.pone.0105732
Selina Kern
Shruti Agarwal
Kilian Huber
Andre P. Gehring
Benjamin Strödke
Christine C. Wirth
Thomas Brügl
Liane Onambele Abodo
Thomas Dandekar
Christian Doerig
Rainer Fischer
Andrew B. Tobin
Mahmood M. Alam
Franz Bracher
Gabriele Pradel
eng
uncontrolled
parasite
eng
uncontrolled
expression
eng
uncontrolled
mosquito
eng
uncontrolled
splicing factors
eng
uncontrolled
lactate dehydrogenase
eng
uncontrolled
xanthurenic acid
eng
uncontrolled
in-vitro
eng
uncontrolled
RNA-SEQ
eng
uncontrolled
identification
eng
uncontrolled
culture
Biowissenschaften; Biologie
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/11540/046_Kern_Plos_One.pdf