32588
2018
eng
14
article
1
2023-08-22
--
--
TMJ pathomorphology in patients with JIA-radiographic parameters for early diagnosis-
Background
Juvenile idiopathic arthritis (JIA) is often accompanied by pathomorphological changes to the temporomandibular joint (TMJ). By analyzing orthodontical orthopantomograms of JIA patients the aims of the study were a) classification of condyle changes, b) quantification of bony asymmetries of condylar destruction and c) detection of relationships between disease duration and TMJ-involvement.
Patients/Methods
46 caucasian JIA-patients (28 female; 18 male; < 16.0 years) were enrolled, each joint (n = 92) was morphologically assessed by means of orthopantomogram, quantitatively analysed and compared with duration of general disease. Condyle morphology was assessed using the Billiau scale for severity of destruction [1]. The quantitative analysis was based on ratios of condyle, ramus and mandible height.
Results
Patients were divided into groups (Group I – slightly affected, n = 36; Billiau severity 0–2; condyle findings: X-ray normal, condyle erosions, condylar flattening; Group II – severely affected, N = 10; Billiau severity 3–4; condyle findings: condylar flattenings and erosions, unilateral/bilateral complete loss of condyles), based on morphological analysis of condylar destruction. Duration of disease was significantly longer in Group II (8.9 ± 5.2 years) than in Group I (4.6 ± 4.7 years). Asymmetries of condyle, ramus and mandible height, quantitatively analysed by contralateral comparison, were significantly more marked in patients of Group II than of Group I.
Conclusions
Orthopantomogram imaging can be used in orthodontics clinical routine to detect TMJ-pathologies and is an important reference for monitoring progression of JIA. Classification into severe and slightly affected TMJ is possible by analysis of condylar pathomorphology. An association between degree of destruction, extent of lower jaw asymmetry and disease duration is suggested by the results.
Head & Face Medicine
10.1186/s13005-018-0173-5
urn:nbn:de:bvb:20-opus-325882
publish
Head & Face Medicine (2018) 14:15. https://doi.org/10.1186/s13005-018-0173-5
false
true
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Daniela Klenke
Anja Quast
Martina Prelog
Annette Holl-Wieden
Maximilian Riekert
Angelika Stellzig-Eisenhauer
Philipp Meyer-Marcotty
eng
uncontrolled
juvenile idiopathic arthritis
eng
uncontrolled
TMJ
eng
uncontrolled
OPT
eng
uncontrolled
asymmetry
eng
uncontrolled
condyle pathomorphology
Medizin und Gesundheit
open_access
Kinderklinik und Poliklinik
Poliklinik für Kieferorthopädie
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/32588/s13005-018-0173-5.pdf
32494
2022
eng
1550-1558
8
52
article
1
--
--
--
Percutaneous implantation of peripherally inserted totally implantable venous access systems in the forearm in adolescent patients
Background
Children with different underlying malignant diseases require long-term central venous access. As for port systems in a pectoral position, peripherally implanted port systems in the forearm revealed high levels of technical and clinical success in adult cohorts.
Objective
To investigate the technical and clinical outcomes of percutaneous central venous port implantation in the forearm in adolescents.
Materials and methods
Between April 2010 and August 2020, 32 children ages 9 to 17 years with underlying malignancy received 35 totally implantable venous access ports (TIVAPs) in the forearm. All venous port systems were peripherally inserted under ultrasound guidance. Correct catheter placement was controlled by fluoroscopy. As primary endpoints, the technical success, rate of complications and catheter maintenance were analyzed. Secondary endpoints were the side of implantation, vein of catheter access, laboratory results on the day of the procedure, procedural radiation exposure, amount of contrast agent and reasons for port device removal.
Results
Percutaneous TIVAP placement under sonographic guidance was technically successful in 34 of 35 procedures (97.1%). Procedure-related complications did not occur. During the follow-up, 13,684 catheter days were analyzed, revealing 11 complications (0.8 per 1,000 catheter-duration days), Of these 11 complications, 7 were major and 10 occurred late. In seven cases, the port device had to be removed; removal-related complications did not occur.
Conclusion
Peripheral TIVAP placement in the forearms of children is a feasible, effective and safe technique with good midterm outcome. As results are comparable with standard access routes, this technique may be offered as an alternative when intermittent venous access is required.
Pediatric Radiology
10.1007/s00247-022-05321-x
urn:nbn:de:bvb:20-opus-324947
@articleAugustin.2022, author = Augustin, Anne Marie and Kertels, Olivia and Wiegering, Verena and Thurner, Annette and Kickuth, Ralph, year = 2022, title = Percutaneous implantation of peripherally inserted totally implantable venous access systems in the forearm in adolescent patients, pages = 1550–1558, volume = 52, number = 8, journal = Pediatric radiology, doi = 10.1007/s00247-022-05321-x
md5:a3d591d2cbfd6d0bcdf8a3f47013cee4
2023-08-12T10:59:36+00:00
/tmp/phpiM66vm
bibtex
64d766188f0ba9.05926436
Pediatric Radiology (2022) 52:8, 1550-1558. DOI: 10.1007/s00247-022-05321-x
false
true
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Anne Marie Augustin
Olivia Kertels
Verena Wiegering
Annette Thurner
Ralph Kickuth
eng
uncontrolled
adolescents
eng
uncontrolled
central venous catheter
eng
uncontrolled
children
eng
uncontrolled
forearm
eng
uncontrolled
interventional radiology
eng
uncontrolled
totally implantable venous access port
eng
uncontrolled
vascular access
Medizin und Gesundheit
open_access
Kinderklinik und Poliklinik
Institut für diagnostische und interventionelle Radiologie (Institut für Röntgendiagnostik)
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/32494/s00247-022-05321-x.pdf
32371
2019
eng
17
article
1
2023-08-11
--
--
Physical activity and health-related quality of life in chronic non-bacterial osteomyelitis
Background
Chronic non-bacterial osteomyelitis (CNO) is an autoinflammatory disorder of the skeletal system of yet unknown etiology. Patients present with local bone pain and inflammation and - to our experience - often suffer from functional impairment with significant disabilities of daily life. The objective of this study was to assess physical activity, fitness and health-related quality of life (HRQOL) in adolescents with established diagnosis of CNO versus healthy controls (HC).
Methods
15 patients with CNO and 15 age and gender matched HC aged 13–18 years, completed questionnaires, performed an incremental exercise test with gas exchange measures up to voluntary fatigue and wore an accelerometer over 7 days at home to assess physical activity behavior.
Results
At the time of assessment, 5 CNO patients were in clinical, one in radiological and 5 in clinical and radiological remission. 7 did not receive any therapy at the time of assessment. The results of the exercise test and of the accelerometry did not show any significant difference between CNO and HC. However, reported sports participation was lower in patients with CNO and PedsQL3.0 and 4.0 showed significant lower values in most of the scores indicating reduced HRQOL.
Conclusion
Although most CNO patients showed a favorable course of disease without any relevant differences in objective measurements of physical activity and fitness versus HC at the time of assessment, questionnaires revealed perceived limitations. Further studies are needed to measure HRQOL and to validate questionnaires in patients with CNO against objective measures including more participants with a higher level of disease activity.
Pediatric Rheumatology
10.1186/s12969-019-0351-4
urn:nbn:de:bvb:20-opus-323710
publish
Pediatric Rheumatology (2019) 17:45. https://doi.org/10.1186/s12969-019-0351-4
false
true
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Julia Nentwich
Katharina Ruf
Hermann Girschick
Annette Holl-Wieden
Henner Morbach
Helge Hebestreit
Christine Hofmann
eng
uncontrolled
chronic non-bacterial osteomyelitis
eng
uncontrolled
CRMO
eng
uncontrolled
HRQOL
eng
uncontrolled
physical activity
Medizin und Gesundheit
open_access
Kinderklinik und Poliklinik
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/32371/s12969-019-0351-4.pdf
32028
2019
eng
8
article
1
2023-06-26
--
--
“Enhanced acquisition of antibiotic-resistant intestinal E. coli during the first year of life assessed in a prospective cohort study”
Background
Increasing bacterial resistance to antibiotics is a serious problem worldwide. We sought to record the acquisition of antibiotic-resistant Escherichia coli (E. coli) in healthy infants in Northern Thailand and investigated potential determinants.
Methods
Stool samples from 142 infants after birth, at ages 2wk, 2mo, 4 to 6mo, and 1y, and parent stool samples were screened for E. coli resistance to tetracycline, ampicillin, co-trimoxazole, and cefazoline by culture, and isolates were further investigated for multiresistance by disc diffusion method. Pulsed-field gel electrophoresis was performed to identify persistent and transmitted strains. Genetic comparison of resistant and transmitted strains was done by multilocus sequence typing (MLST) and strains were further investigated for extra- and intra-intestinal virulence factors by multiplex PCR.
Results
Forty-seven (33%) neonatal meconium samples contained resistant E. coli. Prevalence increased continuously: After 1y, resistance proportion (tetracycline 80%, ampicillin 72%, co-trimoxazole 66%, cefazoline 35%) almost matched those in parents. In 8 infants (6%), identical E. coli strains were found in at least 3 sampling time points (suggesting persistence). Transmission of resistant E. coli from parents to child was observed in only 8 families. MLST showed high diversity. We could not identify any virulence genes or factors associated with persistence, or transmission of resistant E. coli. Full-term, vaginal birth and birth in rural hospital were identified as risk factors for early childhood colonization with resistant E. coli.
Conclusion
One third of healthy Thai neonates harboured antibiotic-resistant E. coli in meconium. The proportion of resistant E. coli increased during the first year of life almost reaching the value in adults. We hypothesize that enhancement of infection control measures and cautious use of antibiotics may help to control further increase of resistance.
Antimicrobial Resistance & Infection Control
10.1186/s13756-019-0522-6
urn:nbn:de:bvb:20-opus-320284
publish
Antimicrobial Resistance & Infection Control (2019) 8:79. https://doi.org/10.1186/s13756-019-0522-6
false
true
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Benjamin Hetzer
Dorothea Orth-Höller
Reinhard Würzner
Peter Kreidl
Michaela Lackner
Thomas Müller
Ludwig Knabl
Daniel Rudolf Geisler-Moroder
Alexander Mellmann
Özcan Sesli
Jeanett Holzknecht
Damia Noce
Noppadon Akarathum
Somporn Chotinaruemol
Martina Prelog
Peninnah Oberdorfer
eng
uncontrolled
Escherichia coli
eng
uncontrolled
antibiotic resistance
eng
uncontrolled
multiresistance
eng
uncontrolled
transmission
eng
uncontrolled
persistence
eng
uncontrolled
children
eng
uncontrolled
neonates
Medizin und Gesundheit
open_access
Kinderklinik und Poliklinik
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/32028/s13756-019-0522-6.pdf
32425
2022
eng
771-782
4
42
article
1
--
--
--
A novel AICDA splice-site mutation in two siblings with HIGM2 permits somatic hypermutation but abrogates mutational targeting
Hyper-IgM syndrome type 2 (HIGM2) is a B cell intrinsic primary immunodeficiency caused by mutations in AICDA encoding activation-induced cytidine deaminase (AID) which impair immunoglobulin class switch recombination (CSR) and somatic hypermutation (SHM). Whereas autosomal-recessive AID-deficiency (AR-AID) affects both CSR and SHM, the autosomal-dominant form (AD-AID) due to C-terminal heterozygous variants completely abolishes CSR but only partially affects SHM. AR-AID patients display enhanced germinal center (GC) reactions and autoimmune manifestations, which are not present in AD-AID, suggesting that SHM but not CSR regulates GC reactions and peripheral B cell tolerance. Herein, we describe two siblings with HIGM2 due to a novel homozygous AICDA mutation (c.428-1G > T) which disrupts the splice acceptor site of exon 4 and results in the sole expression of a truncated AID variant that lacks 10 highly conserved amino acids encoded by exon 4 (AID-ΔE4a). AID-ΔE4a patients suffered from defective CSR and enhanced GC reactions and were therefore indistinguishable from other AR-AID patients. However, the AID-ΔE4a variant only partially affected SHM as observed in AD-AID patients. In addition, AID-ΔE4a but not AD-AID patients revealed impaired targeting of mutational hotspot motives and distorted mutational patterns. Hence, qualitative defects in AID function and altered SHM rather than global decreased SHM activity may account for the disease phenotype in these patients.
Journal of Clinical Immunology
10.1007/s10875-022-01233-5
urn:nbn:de:bvb:20-opus-324253
@articleDirks.2022, author = Dirks, Johannes and Haase, Gabriele and Cantaert, Tineke and Frey, Lea and Klaas, Moritz and Rickert, Christian H. and Girschick, Hermann and Meffre, Eric and Morbach, Henner, year = 2022, title = A Novel AICDA Splice-Site Mutation in Two Siblings with HIGM2 Permits Somatic Hypermutation but Abrogates Mutational Targeting, pages = 771–782, volume = 42, number = 4, journal = Journal of clinical immunology, doi = 10.1007/s10875-022-01233-5
md5:de2abe4e871ca9044689987d9386c648
2023-08-12T10:11:31+00:00
/tmp/phpzrnLpF
bibtex
64d75ad3d98fb2.39431238
Journal of Clinical Immunology (2022) 42:4, 771-782 DOI: 10.1007/s10875-022-01233-5
false
true
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Johannes Dirks
Gabriele Haase
Tineke Cantaert
Lea Frey
Moritz Klaas
Christian H. Rickert
Hermann Girschick
Eric Meffre
Henner Morbach
eng
uncontrolled
hyper-IgM syndrome type 2 (HIGM2)
eng
uncontrolled
AICDA
eng
uncontrolled
AID-ΔE4a
eng
uncontrolled
AD-AID
eng
uncontrolled
mutational targeting
eng
uncontrolled
somatic hypermutation
Medizin und Gesundheit
open_access
Kinderklinik und Poliklinik
Pathologisches Institut
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/32425/s10875-022-01233-5.pdf
32426
2022
eng
767-784
6
44
article
1
--
--
--
Immunization of preterm infants: current evidence and future strategies to individualized approaches
Preterm infants are at particularly high risk for infectious diseases. As this vulnerability extends beyond the neonatal period into childhood and adolescence, preterm infants benefit greatly from infection-preventive measures such as immunizations. However, there is an ongoing discussion about vaccine safety and efficacy due to preterm infants’ distinct immunological features. A significant proportion of infants remains un- or under-immunized when discharged from primary hospital stay. Educating health care professionals and parents, promoting maternal immunization and evaluating the potential of new vaccination tools are important means to reduce the overall burden from infectious diseases in preterm infants. In this narrative review, we summarize the current knowledge about vaccinations in premature infants. We discuss the specificities of early life immunity and memory function, including the role of polyreactive B cells, restricted B cell receptor diversity and heterologous immunity mediated by a cross-reactive T cell repertoire. Recently, mechanistic studies indicated that tissue-resident memory (Trm) cell populations including T cells, B cells and macrophages are already established in the fetus. Their role in human early life immunity, however, is not yet understood. Tissue-resident memory T cells, for example, are diminished in airway tissues in neonates as compared to older children or adults. Hence, the ability to make specific recall responses after secondary infectious stimulus is hampered, a phenomenon that is transcriptionally regulated by enhanced expression of T-bet. Furthermore, the microbiome establishment is a dominant factor to shape resident immunity at mucosal surfaces, but it is often disturbed in the context of preterm birth. The proposed function of Trm T cells to remember benign interactions with the microbiome might therefore be reduced which would contribute to an increased risk for sustained inflammation. An improved understanding of Trm interactions may determine novel targets of vaccination, e.g., modulation of T-bet responses and facilitate more individualized approaches to protect preterm babies in the future.
Seminars in Immunopathology
10.1007/s00281-022-00957-1
urn:nbn:de:bvb:20-opus-324261
@articleFortmann.2022, author = Fortmann, Mats Ingmar and Dirks, Johannes and Goedicke-Fritz, Sybelle and Liese, Johannes and Zemlin, Michael and Morbach, Henner and Härtel, Christoph, year = 2022, title = Immunization of preterm infants: current evidence and future strategies to individualized approaches, pages = 767–784, volume = 44, number = 6, journal = Seminars in immunopathology, doi = 10.1007/s00281-022-00957-1
md5:310bb241ce423aad8a81c2ac07979362
2023-08-12T10:11:31+00:00
/tmp/phpzrnLpF
bibtex
64d75ad3d98fb2.39431238
Seminars in Immunopathology (2022) 44:6, 767-784 DOI: 10.1007/s00281-022-00957-1
false
true
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Mats Ingmar Fortmann
Johannes Dirks
Sybelle Goedicke-Fritz
Johannes Liese
Michael Zemlin
Henner Morbach
Christoph Härtel
eng
uncontrolled
preterm infants
eng
uncontrolled
immunization
eng
uncontrolled
vaccination
eng
uncontrolled
safety
eng
uncontrolled
mechanisms
eng
uncontrolled
resident memory T cells
Medizin und Gesundheit
open_access
Kinderklinik und Poliklinik
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/32426/s00281-022-00957-1.pdf
32428
2023
eng
785-795
2
43
article
1
--
--
--
Ureaplasma-driven neonatal neuroinflammation: novel insights from an ovine model
Ureaplasma species (spp.) are considered commensals of the adult genitourinary tract, but have been associated with chorioamnionitis, preterm birth, and invasive infections in neonates, including meningitis. Data on mechanisms involved in Ureaplasma-driven neuroinflammation are scarce. The present study addressed brain inflammatory responses in preterm lambs exposed to Ureaplasma parvum (UP) in utero. 7 days after intra-amniotic injection of UP (n = 10) or saline (n = 11), lambs were surgically delivered at gestational day 128–129. Expression of inflammatory markers was assessed in different brain regions using qRT-PCR and in cerebrospinal fluid (CSF) by multiplex immunoassay. CSF was analyzed for UP presence using ureB-based real-time PCR, and MRI scans documented cerebral white matter area and cortical folding. Cerebral tissue levels of atypical chemokine receptor (ACKR) 3, caspases 1-like, 2, 7, and C–X–C chemokine receptor (CXCR) 4 mRNA, as well as CSF interleukin-8 protein concentrations were significantly increased in UP-exposed lambs. UP presence in CSF was confirmed in one animal. Cortical folding and white matter area did not differ among groups. The present study confirms a role of caspases and the transmembrane receptors ACKR3 and CXCR4 in Ureaplasma-driven neuroinflammation. Enhanced caspase 1-like, 2, and 7 expression may reflect cell death. Increased ACKR3 and CXCR4 expression has been associated with inflammatory central nervous system (CNS) diseases and impaired blood–brain barrier function. According to these data and previous in vitro findings from our group, we speculate that Ureaplasma-induced caspase and receptor responses affect CNS barrier properties and thus facilitate neuroinflammation.
Cellular and Molecular Neurobiology
10.1007/s10571-022-01213-8
urn:nbn:de:bvb:20-opus-324285
@articleSilwedel.2023, author = Silwedel, Christine and Hütten, Matthias C. and Speer, Christian P. and Härtel, Christoph and Haarmann, Axel and Henrich, Birgit and Tijssen, Maud P. M. and Alnakhli, Abdullah Ahmed and Spiller, Owen B. and Schlegel, Nicolas and Seidenspinner, Silvia and Kramer, Boris W. and Glaser, Kirsten, year = 2023, title = Ureaplasma-Driven Neonatal Neuroinflammation: Novel Insights from an Ovine Model, pages = 785–795, volume = 43, number = 2, journal = Cellular and molecular neurobiology, doi = 10.1007/s10571-022-01213-8
md5:39d5f4c092d05c28b654003d88bc0d7a
2023-08-12T10:11:31+00:00
/tmp/phpzrnLpF
bibtex
64d75ad3d98fb2.39431238
Cellular and Molecular Neurobiology (2023) 43:2, 785-795 DOI: 10.1007/s10571-022-01213-8
false
true
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Christine Silwedel
Matthias C. Hütten
Christian P. Speer
Christoph Härtel
Axel Haarmann
Birgit Henrich
Maud P. M. Tijssen
Abdullah Ahmed Alnakhli
Owen B. Spiller
Nicolas Schlegel
Silvia Seidenspinner
Boris W. Kramer
Kirsten Glaser
eng
uncontrolled
Ureaplasma parvum
eng
uncontrolled
CNS integrity
eng
uncontrolled
neonatal meningitis
eng
uncontrolled
preterm birth
eng
uncontrolled
immaturity
eng
uncontrolled
animal model
Medizin und Gesundheit
open_access
Klinik und Poliklinik für Allgemein-, Viszeral-, Gefäß- und Kinderchirurgie (Chirurgische Klinik I)
Kinderklinik und Poliklinik
Neurologische Klinik und Poliklinik
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/32428/s10571-022-01213-8.pdf
32394
2022
eng
2481-2488
6
407
article
1
--
--
--
Differences in morbidity and mortality between unilateral adrenalectomy for adrenal Cushing’s syndrome and bilateral adrenalectomy for therapy refractory extra-adrenal Cushing’s syndrome
Purpose
In selected cases of severe Cushing’s syndrome due to uncontrolled ACTH secretion, bilateral adrenalectomy appears unavoidable. Compared with unilateral adrenalectomy (for adrenal Cushing’s syndrome), bilateral adrenalectomy has a perceived higher perioperative morbidity. The aim of the current study was to compare both interventions in endogenous Cushing’s syndrome regarding postoperative outcomes.
Methods
We report a single-center, retrospective cohort study comparing patients with hypercortisolism undergoing bilateral vs. unilateral adrenalectomy during 2008–2021. Patients with adrenal Cushing’s syndrome due to adenoma were compared with patients with ACTH-dependent Cushing’s syndrome (Cushing’s disease and ectopic ACTH production) focusing on postoperative morbidity and mortality as well as long-term survival.
Results
Of 83 patients with adrenalectomy for hypercortisolism (65.1% female, median age 53 years), the indication for adrenalectomy was due to adrenal Cushing’s syndrome in 60 patients (72.2%; 59 unilateral and one bilateral), and due to hypercortisolism caused by Cushing’s disease (n = 16) or non-pituitary uncontrolled ACTH secretion of unknown origin (n = 7) (27.7% of all adrenalectomies). Compared with unilateral adrenalectomy (n = 59), patients with bilateral adrenalectomy (n = 24) had a higher rate of severe complications (0% vs. 33%; p < 0.001) and delayed recovery (median: 10.2% vs. 79.2%; p < 0.001). Using the MTL30 marker, patients with bilateral adrenalectomy fared worse than patients after unilateral surgery (MTL30 positive: 7.2% vs. 25.0% p < 0.001). Postoperative mortality was increased in patients with bilateral adrenalectomy (0% vs. 8.3%; p = 0.081).
Conclusion
While unilateral adrenalectomy for adrenal Cushing’s syndrome represents a safe and definitive therapeutic option, bilateral adrenalectomy to control ACTH-dependent extra-adrenal Cushing’s syndrome or Cushing’s disease is a more complicated intervention with a mortality of nearly 10%.
Langenbeck’s Archives of Surgery
10.1007/s00423-022-02568-8
urn:nbn:de:bvb:20-opus-323947
@articleReibetanz.2022, author = Reibetanz, Joachim and Kelm, Matthias and Uttinger, Konstantin L. and Reuter, Miriam and Schlegel, Nicolas and Hankir, Mohamed and Wiegering, Verena and Germer, Christoph-Thomas and Fassnacht, Martin and Lock, Johan Friso and Wiegering, Armin, year = 2022, title = Differences in morbidity and mortality between unilateral adrenalectomy for adrenal Cushing’s syndrome and bilateral adrenalectomy for therapy refractory extra-adrenal Cushing’s syndrome, pages = 2481–2488, volume = 407, number = 6, journal = Langenbeck’s archives of surgery, doi = 10.1007/s00423-022-02568-8
md5:ddfe1c6b2a9e7a3d3e40fcc9aa2aca6c
2023-08-12T09:01:06+00:00
/tmp/phpvihHh5
bibtex
64d74a5205a688.90874886
Langenbeck’s archives of surgery (2022) 407:6, 2481-2488. DOI: 10.1007/s00423-022-02568-8
false
true
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Joachim Reibetanz
Matthias Kelm
Konstantin L. Uttinger
Miriam Reuter
Nicolas Schlegel
Mohamed Hankir
Verena Wiegering
Christoph-Thomas Germer
Martin Fassnacht
Johan Friso Lock
Armin Wiegering
eng
uncontrolled
Cushing
eng
uncontrolled
adrenal surgery
eng
uncontrolled
MTL30
eng
uncontrolled
complication
Chirurgie und verwandte medizinische Fachrichtungen
open_access
Klinik und Poliklinik für Allgemein-, Viszeral-, Gefäß- und Kinderchirurgie (Chirurgische Klinik I)
Kinderklinik und Poliklinik
Theodor-Boveri-Institut für Biowissenschaften
Medizinische Klinik und Poliklinik I
Comprehensive Cancer Center Mainfranken
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/32394/s00423-022-02568-8.pdf
30419
2023
eng
4
15
article
1
--
2023-02-07
--
CAR-T-derived extracellular vesicles: a promising development of CAR-T anti-tumor therapy
Extracellular vesicles (EVs) are a heterogenous population of plasma membrane-surrounded particles that are released in the extracellular milieu by almost all types of living cells. EVs are key players in intercellular crosstalk, both locally and systemically, given that they deliver their cargoes (consisting of proteins, lipids, mRNAs, miRNAs, and DNA fragments) to target cells, crossing biological barriers. Those mechanisms further trigger a wide range of biological responses. Interestingly, EV phenotypes and cargoes and, therefore, their functions, stem from their specific parental cells. For these reasons, EVs have been proposed as promising candidates for EV-based, cell-free therapies. One of the new frontiers of cell-based immunotherapy for the fight against refractory neoplastic diseases is represented by genetically engineered chimeric antigen receptor T (CAR-T) lymphocytes, which in recent years have demonstrated their effectiveness by reaching commercialization and clinical application for some neoplastic diseases. CAR-T-derived EVs represent a recent promising development of CAR-T immunotherapy approaches. This crosscutting innovative strategy is designed to exploit the advantages of genetically engineered cell-based immunotherapy together with those of cell-free EVs, which in principle might be safer and more efficient in crossing biological and tumor-associated barriers. In this review, we underlined the potential of CAR-T-derived EVs as therapeutic agents in tumors.
Cancers
2072-6694
10.3390/cancers15041052
urn:nbn:de:bvb:20-opus-304195
2023-03-14T05:55:09+00:00
sword
swordwue
attachment; filename=deposit.zip
6c98d92f7f28b6bbf9fee04104ec63ff
Cancers (2023) 15:4, 1052. https://doi.org/10.3390/cancers15041052
false
true
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Sara Pagotto
Pasquale Simeone
Davide Brocco
Giulia Catitti
Domenico De Bellis
Simone Vespa
Natalia Di Pietro
Lisa Marinelli
Antonio Di Stefano
Serena Veschi
Laura De Lellis
Fabio Verginelli
Francesco Kaitsas
Manuela Iezzi
Assunta Pandolfi
Rosa Visone
Nicola Tinari
Ignazio Caruana
Mauro Di Ianni
Alessandro Cama
Paola Lanuti
Rosalba Florio
eng
uncontrolled
extracellular vesicles
eng
uncontrolled
CAR-T cells
eng
uncontrolled
tumors
eng
uncontrolled
anti-tumor agents
Medizin und Gesundheit
open_access
Kinderklinik und Poliklinik
Import
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/30419/cancers-15-01052-v2.pdf
32119
2023
eng
12
24
article
1
--
2023-06-19
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Increased expression of anaphylatoxin C5a-receptor-1 in neutrophils and natural killer cells of preterm infants
Preterm infants are susceptible to infection and their defense against pathogens relies largely on innate immunity. The role of the complement system for the immunological vulnerability of preterm infants is less understood. Anaphylatoxin C5a and its receptors C5aR1 and -2 are known to be involved in sepsis pathogenesis, with C5aR1 mainly exerting pro-inflammatory effects. Our explorative study aimed to determine age-dependent changes in the expression of C5aR1 and C5aR2 in neonatal immune cell subsets. Via flow cytometry, we analyzed the expression pattern of C5a receptors on immune cells isolated from peripheral blood of preterm infants (n = 32) compared to those of their mothers (n = 25). Term infants and healthy adults served as controls. Preterm infants had a higher intracellular expression of C5aR1 on neutrophils than control individuals. We also found a higher expression of C5aR1 on NK cells, particularly on the cytotoxic CD56\(^{dim}\) subset and the CD56\(^-\) subset. Immune phenotyping of other leukocyte subpopulations revealed no gestational-age-related differences for the expression of and C5aR2. Elevated expression of C5aR1 on neutrophils and NK cells in preterm infants may contribute to the phenomenon of “immunoparalysis” caused by complement activation or to sustained hyper-inflammatory states. Further functional analyses are needed to elucidate the underlying mechanisms.
International Journal of Molecular Sciences
1422-0067
10.3390/ijms241210321
urn:nbn:de:bvb:20-opus-321196
2023-07-07T08:58:32+00:00
sword
swordwue
attachment; filename=deposit.zip
a3f3c44d4b85ef5ba4c03f3d43ecde50
International Journal of Molecular Sciences (2023) 24:12, 10321. https://doi.org/10.3390/ijms241210321
Interdisziplinäres Zentrum für Klinische Forschung (IZKF)
false
true
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Hannah Boeckel
Christian M. Karsten
Wolfgang Göpel
Egbert Herting
Jan Rupp
Christoph Härtel
Annika Hartz
eng
uncontrolled
preterm infants
eng
uncontrolled
C5a
eng
uncontrolled
C5aR1
eng
uncontrolled
neutrophils
eng
uncontrolled
NK cells
eng
uncontrolled
innate immunity
eng
uncontrolled
sepsis
Medizin und Gesundheit
open_access
Kinderklinik und Poliklinik
Import
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/32119/ijms-24-10321.pdf