12019
2014
eng
35-44
8
article
1
2015-10-13
--
--
Probing the unknowns in cytokinin-mediated immune defense in Arabidopsis with systems biology approaches
Plant hormones involving salicylic acid (SA), jasmonic acid (JA), ethylene (Et), and auxin, gibberellins, and abscisic acid (ABA) are known to regulate host immune responses. However, plant hormone cytokinin has the potential to modulate defense signaling including SA and JA. It promotes plant pathogen and herbivore resistance; underlying mechanisms are still unknown. Using systems biology approaches, we unravel hub points of immune interaction mediated by cytokinin signaling in Arabidopsis. High-confidence Arabidopsis protein-protein interactions (PPI) are coupled to changes in cytokinin-mediated gene expression. Nodes of the cellular interactome that are enriched in immune functions also reconstitute sub-networks. Topological analyses and their specific immunological relevance lead to the identification of functional hubs in cellular interactome. We discuss our identified immune hubs in light of an emerging model of cytokinin-mediated immune defense against pathogen infection in plants.
Bioinformatics and Biology Insights
10.4137/bbi.s13462
1177-9322
24558299
urn:nbn:de:bvb:20-opus-120199
Bioinformatics and Biology Insights 2014:8 35–44 doi: 10.4137/BBI.S13462.
Muhammad Naseem
Meik Kunz
Thomas Dandekar
eng
uncontrolled
plant hormones
eng
uncontrolled
systems biology
eng
uncontrolled
interaction networks
eng
uncontrolled
gene expression
eng
uncontrolled
cytokinin
Biochemie
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/12019/081_Naseem_Bioinformatics_and_Biology_Insights.pdf
11687
2014
eng
715-725
4
99
article
1
2015-07-28
--
--
The reliability of immunohistochemical analysis of the tumor microenvironment in follicular lymphoma: a validation study from the Lunenburg Lymphoma Biomarker Consortium
The cellular microenvironment in follicular lymphoma is of biological and clinical importance. Studies on the clinical significance of non-malignant cell populations have generated conflicting results, which may partly be influenced by poor reproducibility in immunohistochemical marker quantification. In this study, the reproducibility of manual scoring and automated microscopy based on a tissue microarray of 25 follicular lymphomas as compared to flow cytometry is evaluated. The agreement between manual scoring and flow cytometry was moderate for CD3, low for CD4, and moderate to high for CD8, with some laboratories scoring closer to the flow cytometry results. Agreement in manual quantification across the 7 laboratories was low to moderate for CD3, CD4, CD8 and FOXP3 frequencies, moderate for CD21, low for MIB1 and CD68, and high for CD10. Manual scoring of the architectural distribution resulted in moderate agreement for CD3, CD4 and CD8, and low agreement for FOXP3 and CD68. Comparing manual scoring to automated microscopy demonstrated that manual scoring increased the variability in the low and high frequency interval with some laboratories showing a better agreement with automated scores. Manual scoring reliably identified rare architectural patterns of T-cell infiltrates. Automated microscopy analyses for T-cell markers by two different instruments were highly reproducible and provided acceptable agreement with flow cytometry. These validation results provide explanations for the heterogeneous findings on the prognostic value of the microenvironment in follicular lymphoma. We recommend a more objective measurement, such as computer-assisted scoring, in future studies of the prognostic impact of microenvironment in follicular lymphoma patients.
Haematologica
10.3324/haematol.2013.095257
1592-8721
24510338
urn:nbn:de:bvb:20-opus-116875
Haematologica. 2014; 99(4):715-25. doi: 10.3324/haematol.2013.095257
Deutsches Urheberrecht
Brigitta Sander
Daphne de Jong
Andreas Rosenwald
Wanling Xie
Olga Balagué
Maria Calaminici
Joaquim Carreras
Philippe Gaulard
John Gribben
Anton Hagenbeek
Marie José Kersten
Thierry Jo Molina
Abigail Lee
Santiago Montes-Moreno
German Ott
John Raemaekers
Gilles Salles
Laurie Sehn
Christoph Thorns
Bjorn E. Wahlin
Randy D. Gascoyne
Edie Weller
eng
uncontrolled
CD/metabolism
eng
uncontrolled
flow cytometry
eng
uncontrolled
antigens
eng
uncontrolled
regulatory T-cells
eng
uncontrolled
independent predictor
eng
uncontrolled
gene expression
eng
uncontrolled
high numbers
eng
uncontrolled
CD40 ligand
eng
uncontrolled
Riutximab
eng
uncontrolled
survival
eng
uncontrolled
marcophages
eng
uncontrolled
transformation
eng
uncontrolled
in-vitro
Medizin und Gesundheit
open_access
Pathologisches Institut
Universität Würzburg
11688
2014
eng
726-735
4
99
article
Molecular Mechanisms in Malignant Lymphomas (MMML) Network Project
1
2015-07-28
--
--
Biological characterization of adult MYC-translocation-positive mature B-cell lymphomas other than molecular Burkitt lymphoma
Chromosomal translocations affecting the MYC oncogene are the biological hallmark of Burkitt lymphomas but also occur in a subset of other mature B-cell lymphomas. If accompanied by a chromosomal break targeting the BCL2 and/or BCL6 oncogene these MYC translocation-positive (MYC+) lymphomas are called double-hit lymphomas, otherwise the term single-hit lymphomas is applied. In order to characterize the biological features of these MYC+ lymphomas other than Burkitt lymphoma we explored, after exclusion of molecular Burkitt lymphoma as defined by gene expression profiling, the molecular, pathological and clinical aspects of 80 MYC-translocation-positive lymphomas (31 single-hit, 46 double-hit and 3 MYC+-lymphomas with unknown BCL6 status). Comparison of single-hit and double-hit lymphomas revealed no difference in MYC partner (IG/non-IG), genomic complexity, MYC expression or gene expression profile. Double-hit lymphomas more frequently showed a germinal center B-cell-like gene expression profile and had higher IGH and MYC mutation frequencies. Gene expression profiling revealed 130 differentially expressed genes between BCL6(+)/MYC+ and BCL2(+)/MYC+ double-hit lymphomas. BCL2(+)/MYC+ double-hit lymphomas more frequently showed a germinal center B-like gene expression profile. Analysis of all lymphomas according to MYC partner (IG/non-IG) revealed no substantial differences. In this series of lymphomas, in which immunochemotherapy was administered in only a minority of cases, single-hit and double-hit lymphomas had a similar poor outcome in contrast to the outcome of molecular Burkitt lymphoma and lymphomas without the MYC break. Our data suggest that, after excluding molecular Burkitt lymphoma and pediatric cases, MYC+ lymphomas are biologically quite homogeneous with single-hit and double-hit lymphomas as well as IG-MYC and non-IG-MYC+ lymphomas sharing various molecular characteristics.
Haematologica
10.3324/haematol.2013.091827
1592-8721
24179151
urn:nbn:de:bvb:20-opus-116882
Haematologica. 2014; 99(4):726-35. doi: 10.3324/haematol.2013.091827
Deutsches Urheberrecht
Sietse M. Aukema
Markus Kreuz
Christian W. Kohler
Maciej Rosolowski
Dirk Hasenclever
Michael Hummel
Ralf Küppers
Diddo Lenze
German Ott
Christiane Pott
Julia Richter
Andreas Rosenwald
Monika Szczepanowski
Carsten Schwaenen
Harald Stein
Heiko Trautmann
Swen Wessendorf
Lorenz Trümper
Markus Loeffler
Rainer Spang
Philip M. Kluin
Wolfram Klapper
Reiner Siebert
eng
uncontrolled
Rituximab plus
eng
uncontrolled
cyclophsophamide
eng
uncontrolled
c-myc
eng
uncontrolled
gene expression
eng
uncontrolled
genomic aberrations
eng
uncontrolled
follicular lymphoma
eng
uncontrolled
prognostic factor
eng
uncontrolled
poor prognosis
eng
uncontrolled
grade 3B
eng
uncontrolled
distinct
Medizin und Gesundheit
open_access
Pathologisches Institut
Universität Würzburg
11600
2014
eng
e100250
6
9
article
1
2015-07-16
--
--
Novel Method for Analysis of Allele Specific Expression in Triploid Oryzias latipes Reveals Consistent Pattern of Allele Exclusion
Assessing allele-specific gene expression (ASE) on a large scale continues to be a technically challenging problem. Certain biological phenomena, such as X chromosome inactivation and parental imprinting, affect ASE most drastically by completely shutting down the expression of a whole set of alleles. Other more subtle effects on ASE are likely to be much more complex and dependent on the genetic environment and are perhaps more important to understand since they may be responsible for a significant amount of biological diversity. Tools to assess ASE in a diploid biological system are becoming more reliable. Non-diploid systems are, however, not uncommon. In humans full or partial polyploid states are regularly found in both healthy (meiotic cells, polynucleated cell types) and diseased tissues (trisomies, non-disjunction events, cancerous tissues). In this work we have studied ASE in the medaka fish model system. We have developed a method for determining ASE in polyploid organisms from RNAseq data and we have implemented this method in a software tool set. As a biological model system we have used nuclear transplantation to experimentally produce artificial triploid medaka composed of three different haplomes. We measured ASE in RNA isolated from the livers of two adult, triploid medaka fish that showed a high degree of similarity. The majority of genes examined (82%) shared expression more or less evenly among the three alleles in both triploids. The rest of the genes (18%) displayed a wide range of ASE levels. Interestingly the majority of genes (78%) displayed generally consistent ASE levels in both triploid individuals. A large contingent of these genes had the same allele entirely suppressed in both triploids. When viewed in a chromosomal context, it is revealed that these genes are from large sections of 4 chromosomes and may be indicative of some broad scale suppression of gene expression.
PLOS ONE
10.1371/journal.pone.0100250
1932-6203
24945156
urn:nbn:de:bvb:20-opus-116000
PLoS ONE 9(6): e100250. doi:10.1371/journal.pone.0100250
Tzintzuni I. Garcia
Isa Matos
Yingjia Shen
Vagmita Pabuwal
Maria Manuela Coelho
Yuko Wakamatsu
Manfred Schartl
Ronald B. Walter
eng
uncontrolled
RNA-SEQ data
eng
uncontrolled
copy-number alteration
eng
uncontrolled
squalius alburnoides
eng
uncontrolled
gene expression
eng
uncontrolled
medaka
eng
uncontrolled
variant detection
eng
uncontrolled
transplantation
eng
uncontrolled
genome
eng
uncontrolled
generation
eng
uncontrolled
evolution
Medizin und Gesundheit
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/11600/070_Garcia_PLOS_ONE.pdf
11745
2014
eng
e87452
2
9
article
1
2015-08-07
--
--
Bone Marrow-Specific Knock-In of a Non-Activatable Ikkα Kinase Mutant Influences Haematopoiesis but Not Atherosclerosis in Apoe-Deficient Mice
Background: The Ikkα kinase, a subunit of the NF-kappa B-activating IKK complex, has emerged as an important regulator of inflammatory gene expression. However, the role of Ikkα-mediated phosphorylation in haematopoiesis and atherogenesis remains unexplored. In this study, we investigated the effect of a bone marrow (BM)-specific activation-resistant Ikk alpha mutant knock-in on haematopoiesis and atherosclerosis in mice.
Methods and Results: Apolipoprotein E (Apoe)-deficient mice were transplanted with BM carrying an activation-resistant Ikkα gene (Ikkα(AA/AA) Apoe(-/-)) or with Ikkα(+/+) Apoe(-/-) BM as control and were fed a high-cholesterol diet for 8 or 13 weeks. Interestingly, haematopoietic profiling by flow cytometry revealed a significant decrease in B-cells, regulatory T-cells and effector memory T-cells in Ikkα(AA/AA) Apoe(-/-) BM-chimeras, whereas the naive T-cell population was increased. Surprisingly, no differences were observed in the size, stage or cellular composition of atherosclerotic lesions in the aorta and aortic root of Ikkα(AA/AA) Apoe(-/-) vs Ikkα(+/+) Apoe(-/-) BM-transplanted mice, as shown by histological and immunofluorescent stainings. Necrotic core sizes, apoptosis, and intracellular lipid deposits in aortic root lesions were unaltered. In vitro, BM-derived macrophages from Ikkα(AA/AA) Apoe(-/-) vs Ikkα(+/+) Apoe(-/-) mice did not show significant differences in the uptake of oxidized low-density lipoproteins (oxLDL), and, with the exception of Il-12, the secretion of inflammatory proteins in conditions of Tnf-α or oxLDL stimulation was not significantly altered. Furthermore, serum levels of inflammatory proteins as measured with a cytokine bead array were comparable.
Conclusion: Our data reveal an important and previously unrecognized role of haematopoietic Ikkα kinase activation in the homeostasis of B-cells and regulatory T-cells. However, transplantation of Ikkα AA mutant BM did not affect atherosclerosis in Apoe(-/-) mice. This suggests that the diverse functions of Ikkα in haematopoietic cells may counterbalance each other or may not be strong enough to influence atherogenesis, and reveals that targeting haematopoietic Ikkα kinase activity alone does not represent a therapeutic approach.
PLOS ONE
10.1371/journal.pone.0087452
24498325
urn:nbn:de:bvb:20-opus-117450
PLoS ONE 9(2): e87452. doi:10.1371/journal.pone.0087452
Pathricia V. Tilstam
Marion J. Gijbels
Mohamed Habbeddine
Celine Cudejko
Yaw Asare
Wendy Theelen
Baixue Zhou
Yvonne Döring
Maik Drechsler
Lukas Pawig
Sakine Simsekyilmaz
Rory R. Koenen
Menno P. J. de Winther
Toby Lawrence
Jürgen Bernhagen
Alma Zernecke
Christian Weber
Heidi Noels
eng
uncontrolled
NF-KAPPA-B
eng
uncontrolled
regulatory T cells
eng
uncontrolled
indoleamine 2,3-dioxygenase
eng
uncontrolled
dendritic cells
eng
uncontrolled
gene expression
eng
uncontrolled
increases atherosclersosis
eng
uncontrolled
receptor
eng
uncontrolled
inhibition
eng
uncontrolled
pathway
eng
uncontrolled
beta
Medizin und Gesundheit
open_access
Rudolf-Virchow-Zentrum
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/11745/124_Tilstam_PLOS_ONE.pdf
11786
2014
eng
9717-9729
15
42
article
1
2015-08-14
--
--
Regulation of transcription termination by glucosylated hydroxymethyluracil, base J, in Leishmania major and Trypanosoma brucei
Base J, beta-d-glucosyl-hydroxymethyluracil, is an epigenetic modification of thymine in the nuclear DNA of flagellated protozoa of the order Kinetoplastida. J is enriched at sites involved in RNA polymerase ( RNAP) II initiation and termination. Reduction of J in Leishmania tarentolae via growth in BrdU resulted in cell death and indicated a role of J in the regulation of RNAP II termination. To further explore J function in RNAP II termination among kinetoplastids and avoid indirect effects associated with BrdU toxicity and genetic deletions, we inhibited J synthesis in Leishmania major and Trypanosoma brucei using DMOG. Reduction of J in L. major resulted in genome-wide defects in transcription termination at the end of polycistronic gene clusters and the generation of antisense RNAs, without cell death. In contrast, loss of J in T. brucei did not lead to genome-wide termination defects; however, the loss of J at specific sites within polycistronic gene clusters led to altered transcription termination and increased expression of downstream genes. Thus, J regulation of RNAP II transcription termination genome-wide is restricted to Leishmania spp., while in T. brucei it regulates termination and gene expression at specific sites within polycistronic gene clusters.
Nucleic Acids Research
10.1093/nar/gku714
25104019
urn:nbn:de:bvb:20-opus-117863
Nucleic Acids Research, 2014, Vol. 42, No. 15, 9717–9729. doi:10.1093/nar/gku714
David Reynolds
Laura Cliffe
Konrad U. Förstner
Chung-Chau Hon
T. Nicolai Siegel
Robert Sabatini
eng
uncontrolled
RNA-polymerase-II
eng
uncontrolled
variant surface glycoprotein
eng
uncontrolled
SWI2/SNF2-like protein
eng
uncontrolled
messenger RNA
eng
uncontrolled
polycistronic transcription
eng
uncontrolled
DNA glycosation
eng
uncontrolled
hela cells
eng
uncontrolled
gene expression
eng
uncontrolled
genome
eng
uncontrolled
5-bromodeoxyuridine
Medizin und Gesundheit
open_access
Institut für Molekulare Infektionsbiologie
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/11786/146_Reynolds_NUCLEIC_ACIDS_RESEARCH.pdf
11722
2014
eng
2906-2918
5
42
article
1
2015-08-04
--
--
Characterization of two different Asf1 histone chaperones with distinct cellular localizations and functions in Trypanosoma brucei
The anti-silencing function protein 1 (Asf1) is a chaperone that forms a complex with histones H3 and H4 facilitating dimer deposition and removal from chromatin. Most eukaryotes possess two different Asf1 chaperones but their specific functions are still unknown. Trypanosomes, a group of early-diverged eukaryotes, also have two, but more divergent Asf1 paralogs than Asf1 of higher eukaryotes. To unravel possible different functions, we characterized the two Asf1 proteins in Trypanosoma brucei. Asf1A is mainly localized in the cytosol but translocates to the nucleus in S phase. In contrast, Asf1B is predominantly localized in the nucleus, as described for other organisms. Cytosolic Asf1 knockdown results in accumulation of cells in early S phase of the cell cycle, whereas nuclear Asf1 knockdown arrests cells in S/G2 phase. Overexpression of cytosolic Asf1 increases the levels of histone H3 and H4 acetylation. In contrast to cytosolic Asf1, overexpression of nuclear Asf1 causes less pronounced growth defects in parasites exposed to genotoxic agents, prompting a function in chromatin remodeling in response to DNA damage. Only the cytosolic Asf1 interacts with recombinant H3/H4 dimers in vitro. These findings denote the early appearance in evolution of distinguishable functions for the two Asf1 chaperons in trypanosomes.
Nucleic Acids Research
10.1093/nar/gkt1267
1362-4962
24322299
urn:nbn:de:bvb:20-opus-117220
Nucleic Acids Research, 2014, Vol. 42, No. 5, 2906–2918. doi:10.1093/nar/gkt1267
Bruno Pascoalino
Gülcin Dindar
João P. Vieira-da-Rocha
Carlos Renato Machado
Christian J. Janzen
Sergio Schenkman
eng
uncontrolled
chromatin assembly factors
eng
uncontrolled
DNA-damage checkpoint
eng
uncontrolled
tousled-like kinases
eng
uncontrolled
saccharomyes cerevisiae
eng
uncontrolled
gene expression
eng
uncontrolled
acetyltransferase RTT109
eng
uncontrolled
african trypanosomes
eng
uncontrolled
antigenetic variation
eng
uncontrolled
cycle regulation
eng
uncontrolled
nuclear import
Biowissenschaften; Biologie
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/11722/112_Pascoalino_Nucleic_Acids_Research.pdf
11723
2014
eng
3164-3176
5
42
article
1
2015-08-04
--
--
Promoter occupancy of the basal class I transcription factor A differs strongly between active and silent VSG expression sites in Trypanosoma brucei
Monoallelic expression within a gene family is found in pathogens exhibiting antigenic variation and in mammalian olfactory neurons. Trypanosoma brucei, a lethal parasite living in the human bloodstream, expresses variant surface glycoprotein (VSG) from 1 of 15 bloodstream expression sites (BESs) by virtue of a multifunctional RNA polymerase I. The active BES is transcribed in an extranucleolar compartment termed the expression site body (ESB), whereas silent BESs, located elsewhere within the nucleus, are repressed epigenetically. The regulatory mechanisms, however, are poorly understood. Here we show that two essential subunits of the basal class I transcription factor A (CITFA) predominantly occupied the promoter of the active BES relative to that of a silent BES, a phenotype that was maintained after switching BESs in situ. In these experiments, high promoter occupancy of CITFA was coupled to high levels of both promoter-proximal RNA abundance and RNA polymerase I occupancy. Accordingly, fluorescently tagged CITFA-7 was concentrated in the nucleolus and the ESB. Because a ChIP-seq analysis found that along the entire BES, CITFA-7 is specifically enriched only at the promoter, our data strongly indicate that monoallelic BES transcription is activated by a mechanism that functions at the level of transcription initiation.
Nucleic Acid Research
10.1093/nar/gkt1301
1362-4962
24353315
urn:nbn:de:bvb:20-opus-117232
Nucleic Acids Research, 2014, Vol. 42, No. 5, 3164-3176. doi:10.1093/nar/gkt1301
Research Center of Infectious Diseases (ZINF) of the University of Wurzburg, Germany
Tu N. Nguyen
Laura S. M. Müller
Sung Hee Park
T. Nicolai Siegel
Arthur Günzl
eng
uncontrolled
RNA-polymerase-I
eng
uncontrolled
blood-stream forms
eng
uncontrolled
acrican trypanosomes
eng
uncontrolled
gene expression
eng
uncontrolled
antigenic variation
eng
uncontrolled
ribosomal RNA
eng
uncontrolled
plasmodium falciparum
eng
uncontrolled
virulence genes
eng
uncontrolled
subunit
eng
uncontrolled
complex
Medizin und Gesundheit
open_access
Institut für Molekulare Infektionsbiologie
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/11723/113_Nguyen_Nucleic_Acids_Research.pdf
11728
2014
eng
e89187
2
9
article
1
2015-08-04
--
--
Miz1 Deficiency in the Mammary Gland Causes a Lactation Defect by Attenuated Stat5 Expression and Phosphorylation
Miz1 is a zinc finger transcription factor with an N-terminal POZ domain. Complexes with Myc, Bcl-6 or Gfi-1 repress expression of genes like Cdkn2b (p15(Ink4)) or Cd-kn1a (p21(Cip1)). The role of Miz1 in normal mammary gland development has not been addressed so far. Conditional knockout of the Miz1 POZ domain in luminal cells during pregnancy caused a lactation defect with a transient reduction of glandular tissue, reduced proliferation and attenuated differentiation. This was recapitulated in vitro using mouse mammary gland derived HC11 cells. Further analysis revealed decreased Stat5 activity in Miz1 Delta POZ mammary glands and an attenuated expression of Stat5 targets. Gene expression of the Prolactin receptor (PrlR) and ErbB4, both critical for Stat5 phosphorylation (pStat5) or pStat5 nuclear translocation, was decreased in Miz1 Delta POZ females. Microarray, ChIP-Seq and gene set enrichment analysis revealed a down-regulation of Miz1 target genes being involved in vesicular transport processes. Our data suggest that deranged intracellular transport and localization of PrlR and ErbB4 disrupt the Stat5 signalling pathway in mutant glands and cause the observed lactation phenotype.
PLOS ONE
10.1371/journal.pone.0089187
24586582
urn:nbn:de:bvb:20-opus-117286
PLoS ONE 9(2): e89187. doi:10.1371/journal.pone.0089187
Adrian Sanz-Moreno
David Fuhrmann
Elmar Wolf
Björn von Eyss
Martin Eilers
Hans-Peter Elsässer
eng
uncontrolled
C-MYC
eng
uncontrolled
transcription factor MIZ-1
eng
uncontrolled
breast-cancer cells
eng
uncontrolled
gene expression
eng
uncontrolled
epithelial cells
eng
uncontrolled
prolactin
eng
uncontrolled
transgenic mice
eng
uncontrolled
growth
eng
uncontrolled
differentiation
eng
uncontrolled
proliferation
Biowissenschaften; Biologie
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/11728/117_Sanz-Moreno_PLOS_ONE.pdf
11464
2014
eng
e112946
11
9
article
1
2015-06-20
--
--
The Arabidopsis PLAT Domain Protein1 is Critically Involved in Abiotic Stress Tolerance
Despite the completion of the Arabidopsis genome sequence, for only a relatively low percentage of the encoded proteins experimental evidence concerning their function is available. Plant proteins that harbour a single PLAT (Polycystin, Lipoxygenase, Alpha-toxin and Triacylglycerol lipase) domain and belong to the PLAT-plant-stress protein family are ubiquitously present in monocot and dicots. However, the function of PLAT-plant-stress proteins is still poorly understood. Therefore, we have assessed the function of the uncharacterised Arabidopsis PLAT-plant-stress family members through a combination of functional genetic and physiological approaches. PLAT1 overexpression conferred increased abiotic stress tolerance, including cold, drought and salt stress, while loss-of-function resulted in opposite effects on abiotic stress tolerance. Strikingly, PLAT1 promoted growth under non-stressed conditions. Abiotic stress treatments induced PLAT1 expression and caused expansion of its expression domain. The ABF/ABRE transcription factors, which are positive mediators of abscisic acid signalling, activate PLAT1 promoter activity in transactivation assays and directly bind to the ABRE elements located in this promoter in electrophoretic mobility shift assays. This suggests that PLAT1 represents a novel downstream target of the abscisic acid signalling pathway. Thus, we showed that PLAT1 critically functions as positive regulator of abiotic stress tolerance, but also is involved in regulating plant growth, and thereby assigned a function to this previously uncharacterised PLAT domain protein. The functional data obtained for PLAT1 support that PLAT-plant-stress proteins in general could be promising targets for improving abiotic stress tolerance without yield penalty.
PLOS ONE
10.1371/journal.pone.0112946
25396746
urn:nbn:de:bvb:20-opus-114648
PLoS ONE 9(11): e112946. doi:10.1371/journal.pone.0112946
Tae Kyung Hyun
Eric van der Graaff
Alfonso Albacete
Seung Hee Eom
Dominik K. Grosskinsky
Hannah Böhm
Ursula Janschek
Yeonggil Rim
Walid Wahid Ali
Soo Young Kim
Thomas Roitsch
eng
uncontrolled
salicylic acid
eng
uncontrolled
gene expression
eng
uncontrolled
signal transduction
eng
uncontrolled
cold stress
eng
uncontrolled
salt stress
eng
uncontrolled
abscisic acid
eng
uncontrolled
endoplasmatic reticulum
eng
uncontrolled
transcription factors
eng
uncontrolled
pseudomonas syringae
eng
uncontrolled
plants response
Pflanzen (Botanik)
open_access
Julius-von-Sachs-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/11464/017_Hyun_PLOS_ONE.pdf