7266
1991
eng
bookpart
1
2013-09-03
--
--
Genetic factors in tumour formation: The melanoma-inducing gene of Xiphophorus
No abstract available.
8067
urn:nbn:de:bvb:20-opus-86388
In: Molecular biology intumour research / ed. Olaf-Georg Issinger. - Heidelberg : Hüthig, 1991. - S. 79-94. - (BioTechForum 5: Adv. Molecular Genetics ; 3)
Deutsches Urheberrecht
D. Adam
A. Schartl
S. Andexinger
S. Hölter
B. Wilde
Manfred Schartl
deu
swd
Humangenetik
deu
swd
Tumor
deu
swd
Entstehung
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/7266/Schartl_7266.pdf
5936
1988
eng
article
1
2012-06-25
--
--
RFLP for an EGF-receptor related gene associated with the melanoma oncogene locus of Xiphophorus maculatus
No abstract available
urn:nbn:de:bvb:20-opus-61822
6182
In: Nucleic Acids Research (1988 ) 16, 7212.
Deutsches Urheberrecht
D. Adam
J. Wittbrodt
A. Telling
Manfred Schartl
deu
swd
Physiologische Chemie
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/5936/Schartl42.pdf
5337
1993
eng
article
1
2011-12-07
--
--
Tumor suppression in Xiphophorus by an accidentally acquired promoter
Melanoma formation in the teleost Xiphophorus is caused by a dominant genetic locus, Tu. This locus includes the Xmrk oncogene, which encodes a receptor tyrosine kinase. Tumor induction is. suppressed in wild-type fish by a tumor suppressor locus, R. Molecular genetic analyses revealed that the Tu locus emerged by nonhomologaus recombination of the Xmrk proto-oncogene with a previously uncharacterized sequence, D. This event generated an additional copy of Xmrk with a new promoter. Suppression of the new Xmrk promoter by R in parental fish and its deregulation in hybrids explain the genetics of melanoma formation in Xiphophorus.
urn:nbn:de:bvb:20-opus-61630
6163
In: Science (1993) , 259, 816-819
Deutsches Urheberrecht
Dieter Adam
Nicola Dimitrijevic
Manfred Schartl
deu
swd
Physiologische Chemie
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/5337/Schartl11.pdf
7193
1991
eng
article
1
2013-08-22
--
--
Transcriptional activation of the melanoma inducing Xmrk oncogene in Xiphophorus
The melanoma inducing locus of Xiphophorus encodes a tumorigenic version of a novel putative receptor tyrosine kinase (Xmrk). To elucidate the mechanism of oncogenic activation of Xmrk, we compared the structure and expression of two oncogenic loci with the corresponding proto-oncogene. Only minor structural alterations were found to be specific for the oncogenic Xmrk genes. Marked overexpression of the oncogene transcripts in melanoma, which are approximately 1 kb shorter than the proto-oncogene transcript, correlates with the malignancy of the tumors. The tumor transcripts are derived from an alternative transcription start site that is used only in the oncogenic loci. Thus, oncogenic activation of the melanoma inducing Xmrk gene appears primarily to be due to novel transcriptional control and overexpression.
8033
urn:nbn:de:bvb:20-opus-87584
In: Oncogene, 1991, 6, S. 73-80
Deutsches Urheberrecht
Dieter Adam
Winfried Maueler
Manfred Schartl
deu
swd
Schwertkärpfling
deu
swd
Onkogen
deu
swd
Melanom
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/7193/Schartl_7193.pdf
12648
2015
eng
2
13
article
1
2016-01-31
--
--
Molecular cloning and expression analysis of dmrt1 and sox9 during gonad development and male reproductive cycle in the lambari fish, Astyanax altiparanae
Background
The dmrt1 and sox9 genes have a well conserved function related to testis formation in vertebrates, and the group of fish presents a great diversity of species and reproductive mechanisms. The lambari fish (Astyanax altiparanae) is an important Neotropical species, where studies on molecular level of sex determination and gonad maturation are scarce.
Methods
Here, we employed molecular cloning techniques to analyze the cDNA sequences of the dmrt1 and sox9 genes, and describe the expression pattern of those genes during development and the male reproductive cycle by qRT-PCR, and related to histology of the gonad.
Results
Phylogenetic analyses of predicted amino acid sequences of dmrt1 and sox9 clustered A. altiparanae in the Ostariophysi group, which is consistent with the morphological phylogeny of this species. Studies of the gonad development revealed that ovary formation occurred at 58 days after hatching (dah), 2 weeks earlier than testis formation. Expression studies of sox9 and dmrt1 in different tissues of adult males and females and during development revealed specific expression in the testis, indicating that both genes also have a male-specific role in the adult. During the period of gonad sex differentiation, dmrt1 seems to have a more significant role than sox9. During the male reproductive cycle dmrt1 and sox9 are down-regulated after spermiation, indicating a role of these genes in spermatogenesis.
Conclusions
For the first time the dmrt1 and sox9 were cloned in a Characiformes species. We show that both genes have a conserved structure and expression, evidencing their role in sex determination, sex differentiation and the male reproductive cycle in A. altiparanae. These findings contribute to a better understanding of the molecular mechanisms of sex determination and differentiation in fish.
Reproductive Biology and Endocrinology
10.1186/1477-7827-13-2
urn:nbn:de:bvb:20-opus-126486
Reproductive Biology and Endocrinology 2015, 13:2. DOI:10.1186/1477-7827-13-2
Mateus C. Adolfi
Ana C. O. Carreira
Lázaro W. O. Jesus
Jan Bogerd
Rejane M. Funes
Manfred Schartl
Mari C. Sogayar
Maria I. Borella
eng
uncontrolled
spermatogenesis
eng
uncontrolled
SOX9
eng
uncontrolled
DMRT1
eng
uncontrolled
sex differentiation
eng
uncontrolled
teleostei
Humanphysiologie
open_access
Theodor-Boveri-Institut für Biowissenschaften
Förderzeitraum 2015
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/12648/Adolfi_ReproductiveBiology_1477-7827-13-2.pdf
26567
2021
eng
1
11
article
1
2022-04-05
--
--
A duplicated copy of id2b is an unusual sex-determining candidate gene on the Y chromosome of arapaima (Arapaima gigas)
Arapaima gigas is one of the largest freshwater fish species of high ecological and economic importance. Overfishing and habitat destruction are severe threats to the remaining wild populations. By incorporating a chromosomal Hi-C contact map, we improved the arapaima genome assembly to chromosome-level, revealing an unexpected high degree of chromosome rearrangements during evolution of the bonytongues (Osteoglossiformes). Combining this new assembly with pool-sequencing of male and female genomes, we identified id2bbY, a duplicated copy of the inhibitor of DNA binding 2b (id2b) gene on the Y chromosome as candidate male sex-determining gene. A PCR-test for id2bbY was developed, demonstrating that this gene is a reliable male-specific marker for genotyping. Expression analyses showed that this gene is expressed in juvenile male gonads. Its paralog, id2ba, exhibits a male-biased expression in immature gonads. Transcriptome analyses and protein structure predictions confirm id2bbY as a prime candidate for the master sex-determiner. Acting through the TGF beta signaling pathway, id2bbY from arapaima would provide the first evidence for a link of this family of transcriptional regulators to sex determination. Our study broadens our current understanding about the evolution of sex determination genetic networks and provide a tool for improving arapaima aquaculture for commercial and conservation purposes.
Scientific Reports
10.1038/s41598-021-01066-z
urn:nbn:de:bvb:20-opus-265672
publish
Scientific Reports (2021) 11:1, 21544. https://doi.org/10.1038/s41598-021-01066-z
false
true
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Mateus C. Adolfi
Kang Du
Susanne Kneitz
Cédric Cabau
Margot Zahm
Christophe Klopp
Romain Feron
Rômulo V. Paixão
Eduardo S. Varela
Fernanda L. de Almeida
Marcos A. de Oliveira
Rafael H. Nóbrega
Céline Lopez-Roques
Carole Iampietro
Jérôme Lluch
Werner Kloas
Sven Wuertz
Fabian Schaefer
Matthias Stöck
Yann Guiguen
Manfred Schartl
eng
uncontrolled
evolutionary genetics
eng
uncontrolled
genetic markers
eng
uncontrolled
genome
Medizin und Gesundheit
open_access
Theodor-Boveri-Institut für Biowissenschaften
Förderzeitraum 2021
Comprehensive Cancer Center Mainfranken
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/26567/s41598-021-01066-z.pdf
22266
2021
eng
8
article
1
--
2021-01-18
--
Crosstalk Between Retinoic Acid and Sex-Related Genes Controls Germ Cell Fate and Gametogenesis in Medaka
Sex determination (SD) is a highly diverse and complex mechanism. In vertebrates, one of the first morphological differences between the sexes is the timing of initiation of the first meiosis, where its initiation occurs first in female and later in male. Thus, SD is intimately related to the responsiveness of the germ cells to undergo meiosis in a sex-specific manner. In some vertebrates, it has been reported that the timing for meiosis entry would be under control of retinoic acid (RA), through activation of Stra8. In this study, we used a fish model species for sex determination and lacking the stra8 gene, the Japanese medaka (Oryzias latipes), to investigate the connection between RA and the sex determination pathway. Exogenous RA treatments act as a stress factor inhibiting germ cell differentiation probably by activation of dmrt1a and amh. Disruption of the RA degrading enzyme gene cyp26a1 induced precocious meiosis and oogenesis in embryos/hatchlings of female and even some males. Transcriptome analyzes of cyp26a1–/–adult gonads revealed upregulation of genes related to germ cell differentiation and meiosis, in both ovaries and testes. Our findings show that germ cells respond to RA in a stra8 independent model species. The responsiveness to RA is conferred by sex-related genes, restricting its action to the sex differentiation period in both sexes.
Frontiers in Cell and Developmental Biology
2296-634X
10.3389/fcell.2020.613497
urn:nbn:de:bvb:20-opus-222669
2021-02-01T06:19:12+00:00
sword
swordwue
attachment; filename=deposit.zip
214025c86118ba916d6e8e030800e0e3
Frontiers in Cell and Developmental Biology 2021, 8:613497. doi: 10.3389/fcell.2020.613497
true
true
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Mateus C. Adolfi
Amaury Herpin
Anabel Martinez-Bengochea
Susanne Kneitz
Martina Regensburger
David J. Grunwald
Manfred Schartl
eng
uncontrolled
sex determination
eng
uncontrolled
retinoic acid
eng
uncontrolled
meiosis
eng
uncontrolled
gametogenesis
eng
uncontrolled
medaka
Medizin und Gesundheit
open_access
Theodor-Boveri-Institut für Biowissenschaften
Import
Förderzeitraum 2020
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/22266/fcell-08-613497.pdf
14784
2016
eng
34281
6
article
1
2017-05-05
--
--
Retinoic acid and meiosis induction in adult versus embryonic gonads of medaka
In vertebrates, one of the first recognizable sex differences in embryos is the onset of meiosis, known to be regulated by retinoic acid (RA) in mammals. We investigated in medaka a possible meiotic function of RA during the embryonic sex determination (SD) period and in mature gonads. We found RA mediated transcriptional activation in germ cells of both sexes much earlier than the SD stage, however, no such activity during the critical stages of SD. In adults, expression of the RA metabolizing enzymes indicates sexually dimorphic RA levels. In testis, RA acts directly in Sertoli, Leydig and pre-meiotic germ cells. In ovaries, RA transcriptional activity is highest in meiotic oocytes. Our results show that RA plays an important role in meiosis induction and gametogenesis in adult medaka but contrary to common expectations, not for initiating the first meiosis in female germ cells at the SD stage.
Scientific Reports
10.1038/srep34281
urn:nbn:de:bvb:20-opus-147843
Scientific Reports 6, 34281; doi: 10.1038/srep34281 (2016)
Mateus C. Adolfi
Amaury Herpin
Martina Regensburger
Jacopo Sacquegno
Joshua S. Waxman
Manfred Schartl
eng
uncontrolled
developmental biology
eng
uncontrolled
molecular biology
Genetik und Evolution
open_access
Theodor-Boveri-Institut für Biowissenschaften
Förderzeitraum 2016
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/14784/Adolfi_srep34281.pdf
6975
1994
eng
bookpart
1
2013-08-23
--
--
Genetics and molecular biology of tumour formation in Xiphophorus
No abstract available.
8052
urn:nbn:de:bvb:20-opus-69752
In: The Legacy of Cell Fusion / ed. by Siamon Gordon. - Oxford [u.a.]: Oxford Univ. Press, 1994. - S. 199-214. - ISBN 0-19-854772-2
Deutsches Urheberrecht
Joachim Altschmied
Manfred Schartl
deu
swd
Schwertkärpfling
deu
swd
Tumor
deu
swd
Entstehung
deu
swd
Molekularbiologie
deu
swd
Genetik
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/6975/Schartl_6975.pdf
7270
1985
eng
article
1
2013-09-03
--
--
The genes that carcinogens act upon
No abstract available.
8071
urn:nbn:de:bvb:20-opus-72704
In: Haematology and Blood Transfusion, 1985, 29, S. 228-52
Deutsches Urheberrecht
F. Anders
Manfred Schartl
A. Barnekow
C. R. Schmidt
W. Luke
G. Jaenel-Dess
A. Anders
deu
swd
Onkogen
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/7270/Schartl_7270.pdf
7274
1981
eng
conferenceobject
1
2013-09-03
--
--
Evaluation of environmental and hereditary factors in carcinogenesis, based on studies in Xiphophorus
Neoplasia in Xiphophorus can be classified into a) a large group that is triggered by carcinogens; b) a large group triggered by promoters; c) a small group that develops "spontaneously" following interpopulational and interracial hybridizations; and d) a small group that develops "spontaneously" following germ line mutation. The process leading to susceptibility for neoplasia is represented by the disintegration of gene systems that normally protect the fish from neoplasia. Hybridization is the most effective process that leads to disintegration of the protection gene systems. Environmental factors may complete disintegration and thus may trigger neoplasia. It is discussed whether the findings on Xiphophorus may also apply to humans.
8075
urn:nbn:de:bvb:20-opus-72741
In: Phyletic Approaches to Cancer / ed. by. Clyde J. Dawe. - Tokyo: Japan Scientific Soc. Press, 1981. - S. 289-309. - (Proceedings of the international symposium of the Princess Takamatsu Cancer Research Fund ; 11)
Deutsches Urheberrecht
F. Anders
Manfred Schartl
E. Scholl
deu
swd
Schwertkärpfling
deu
swd
Gen
deu
swd
Umweltfaktor
deu
swd
Carcinogenese
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/7274/Schartl_7274.pdf
7273
1981
eng
conferenceobject
1
2013-09-03
--
--
Environmental and hereditary factors in the causation of neoplasia, based on studies of the Xiphophorus fish melanoma system
Neoplasia in Xiphophorus can be classified into: a) a Jarge group triggered by carcinogens; b) a large group triggered by promoters; and c) a small group that develops "spontaneously" according to Mendelian Jaw. The process leading to susceptibility for neoplasia is represented by the disintegration of gene systems that normally protect the fish from neoplasia. Interpopulational arid interracial hybridization is the most effective process that Ieads to disintegration of the protective gene systems. Environmental factors may complete disintegration in somatic cells and thus may trigger neoplasia. The applications of the findings on Xiphophorus to humans are discussed.
8074
urn:nbn:de:bvb:20-opus-86402
In: Phenotypic Expression in Pigment Cells: Pigment Cell / ed. Makoto Seiji. - [Tokyo]: Univ. of Tokyo Press, 1981. - S. 491-499. - ISBN: 0-86008-293-8
Deutsches Urheberrecht
F. Anders
E. Scholl
Manfred Schartl
deu
swd
Schwertkärpfling
deu
swd
Gen
deu
swd
Umweltfaktor
deu
swd
Tumor
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/7273/Schartl_7273.pdf
7275
1979
deu
bookpart
1
2013-09-03
--
--
Xiphophorus als Modell in der Krebsforschung
No abstract available.
8076
urn:nbn:de:bvb:20-opus-72752
In: Organo- und Immunotherapie: Neue Perspektiven in der Medizin / hrsg. Harald Pocher. - Stuttgart: Enke, 1979. - S. 38-100. - ISBN 3-432-90851-2
Deutsches Urheberrecht
F. Anders
E. Scholl
Manfred Schartl
deu
swd
Schwertkärpfling
deu
swd
Krebsforschung
deu
swd
Modell
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/7275/Schartl_7275.pdf
7272
1984
eng
conferenceobject
1
2013-09-03
--
--
Xiphophorus as an in vivo model for studies on oncogenes
The capacity of Xiphophorus to develop neoplasia can be formally assigned to a "tumor gene" (Tu), which appears to be a normal part of the genome of all individuals. The wild fish have evolved population-specific and cell type-specific systems of regulatory genes (R) for Tu that protect the fish from neoplasia. Hybridization of members of different wild populations in the laborstory followed by treatment of the hybrids with carcinogens led to disintegration of the R systems permitting excessive expression of Tu and thus resulting in neoplasia. Certain hybrids developed neoplasia even spontaneously. Observations on the genuine phenotypic effect of the derepressed Tu in the early embryo indicated an essential normal function of this oncogene in cell differentiation, proliferation and cell-cell communication. Tu appeared to be indispensable in the genome but may also be present in accessory copics. Recently, c-src, the cellular homolog of the Rous sarcoma virus oncogene v-src, was detected in Xiphophorus. The protein product of c-src, pp60c-src, was identified and then examined by its associated kinase activity. This pp60c-src was found in all individuals tested, but, depending on the genotype, its kinase activity was different. The genetic characters of c-src, such as linkage relations, dosage relations, expression, etc., correspond to those of Tu. From a systematic study which showed that pp60c-src was present in all metazoa tested ranging from mammals down to sponges, we concluded that c-src has evolved with the multicellular organization of animals. Neoplasia of animals and humans is a characteristic closely related to this evolution. Our data showed that small aquariurn fish, besides being used successfully because they are time-, space-, and money-saving systems for carcinogenicity testing, are also highly suitable for basic studies on neoplasia at the populational, morphological, developmental, cell biological, and molecular levels.
8073
urn:nbn:de:bvb:20-opus-86398
In: Use of small fish species in carcinogenity testing / ed. Karen L. Hoover. - Bethesda, Md: US Dep. of Health and Human Services, Public Health Service, National Institutes of Health; 1984. - S. 97-109. - (National Cancer Institute <Bethesda, Md.>: Monographs ; 65)
Deutsches Urheberrecht
Fritz Anders
Manfred Schartl
Angelika Barnekow
deu
swd
Schwertkärpfling
deu
swd
In vivo
deu
swd
Onkogen
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/7272/Schartl_7272.pdf
19696
2018
eng
675
9
article
1
--
2018-12-18
--
Ras-Induced miR-146a and 193a Target Jmjd6 to Regulate Melanoma Progression
Ras genes are among the most commonly mutated genes in human cancer; yet our understanding of their oncogenic activity at the molecular mechanistic level is incomplete. To identify downstream events that mediate ras-induced cellular transformation in vivo, we analyzed global microRNA expression in three different models of Ras-induction and tumor formation in zebrafish. Six microRNAs were found increased in Ras-induced melanoma, glioma and in an inducible model of ubiquitous Ras expression. The upregulation of the microRNAs depended on the activation of the ERK and AKT pathways and to a lesser extent, on mTOR signaling. Two Ras-induced microRNAs (miR-146a and 193a) target Jmjd6, inducing downregulation of its mRNA and protein levels at the onset of Ras expression during melanoma development. However, at later stages of melanoma progression, jmjd6 levels were found elevated. The dynamic of Jmjd6 levels during progression of melanoma in the zebrafish model suggests that upregulation of the microRNAs targeting Jmjd6 may be part of an anti-cancer response. Indeed, triple transgenic fish engineered to express a microRNA-resistant Jmjd6 from the onset of melanoma have increased tumor burden, higher infiltration of leukocytes and shorter melanoma-free survival. Increased JMJD6 expression is found in several human cancers, including melanoma, suggesting that the up-regulation of Jmjd6 is a critical event in tumor progression.
The following link has been created to allow review of record GSE37015: http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?token=jjcrbiuicyyqgpc&acc=GSE37015.
Frontiers in Genetics
1664-8021
10.3389/fgene.2018.00675
urn:nbn:de:bvb:20-opus-196963
Frontiers in Genetics (2018) 9:675. doi: 10.3389/fgene.2018.00675
667787
037220
false
true
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Viviana Anelli
Anita Ordas
Susanne Kneitz
Leonel Munoz Sagredo
Victor Gourain
Manfred Schartl
Annemarie H. Meijer
Marina Mione
eng
uncontrolled
zebrafish
eng
uncontrolled
cancer models
eng
uncontrolled
microRNA
eng
uncontrolled
Jmjd6
eng
uncontrolled
ras
eng
uncontrolled
melanoma
eng
uncontrolled
miR-146a
eng
uncontrolled
miR-193a
Medizin und Gesundheit
open_access
Theodor-Boveri-Institut für Biowissenschaften
OpenAIRE
Import
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/19696/fgene-09-00675.pdf
5941
1987
eng
article
1
2012-06-25
--
--
Expression of the c-src protooncogene in human skin tumors
No abstract available
urn:nbn:de:bvb:20-opus-61870
6187
In: Cancer Research (1987) 47, 235-40.
Deutsches Urheberrecht
A. Barnekow
E. Paul
Manfred Schartl
deu
swd
Physiologische Chemie
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/5941/Schartl50.pdf
5940
1987
eng
article
1
2012-06-25
--
--
Comparative studies on the src proto-oncogene and its gene product pp60\(^{c-src}\) in normal and neoplastic tissues of lower vertebrates
No abstract available
urn:nbn:de:bvb:20-opus-61869
6186
Comparative Biochemistry and Physiology / Part B (1987) 87, 663-70.
Deutsches Urheberrecht
A. Barnekow
Manfred Schartl
deu
swd
Physiologische Chemie
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/5940/Schartl48.pdf
5952
1982
eng
article
1
2012-06-28
--
--
Identification of a fish protein associated with a kinase activity and related to the Rous sarcoma virus transforming protein
No abstract available
urn:nbn:de:bvb:20-opus-61946
6194
In: Cancer Research (1982) 42, 2429-33.
Deutsches Urheberrecht
A. Barnekow
Manfred Schartl
F. Anders
H. Bauer
deu
swd
Physiologische Chemie
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/5952/Schartl62.pdf
7075
1990
eng
article
1
2013-08-22
--
--
Synaptophysin: a substrate for the protein tyrosine kinase pp60c-src in intact synaptic vesicles
Expression of pp60 c-src, the first well defined proto-oncogene product, is developmentally regulated and tissue-specific, with neuronal tissues displaying high amounts of the c-src encoded pp60 c-src kinase activity. In the central nervous system pp60 s-src is preferentially expressed in regions characterized by a high content of grey matter and elevated density of nerve terminals. In this study we show for the first time a direct interaction between pp60 c-src and synaptophysin as a physiological target protein in neurons by demonstrating that endogenous pp60 c-src is able to phosphorylate synaptophysin (p38). p38 is a major constituent of the synaptic vesicle membrane protein and is thought to play a key role in the exocytosis of small synaptic vesicles and possibly small clear vesicles in neuroendocrine cells.
8036
urn:nbn:de:bvb:20-opus-86168
In: Oncogene, 1990, 5, S. 1019-1024
Deutsches Urheberrecht
Angelika Barnekow
Reinhard Jahn
Manfred Schartl
deu
swd
Synaptophysin
deu
swd
Synaptische Vesikel
deu
swd
Protein-Tyrosin-Kinasen
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/7075/Schartl_7075.pdf
5931
1989
eng
article
1
2012-06-19
--
--
Structure and expression of the murine retinoblastoma gene and characterization of its encoded protein
No abstract available
urn:nbn:de:bvb:20-opus-61819
6181
In: Proceedings of the National Academy of Sciences of the United States of America (1989) 86, 6474-8.
Deutsches Urheberrecht
R. Bernards
G. M. Schackleford
M. R. Gerber
J. M. Horowitz
S. H. Friend
Manfred Schartl
E. Bogenmann
J. M. Rapaport
T. Mcgee
T. P. Dryja
deu
swd
Physiologische Chemie
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/5931/Schartl40.pdf
18878
2016
eng
1151-1154
11
35
article
1
2019-10-15
--
--
Considerations for a European animal welfare standard to evaluate adverse phenotypes in teleost fish
No abstract available.
The EMBO Journal
10.15252/embj.201694448
urn:nbn:de:bvb:20-opus-188783
The EMBO Journal (2016) 35:11, 1151-1154. https://doi.org/10.15252/embj.201694448
false
true
CC BY-NC-ND: Creative-Commons-Lizenz: Namensnennung, Nicht kommerziell, Keine Bearbeitungen 4.0 International
Bettina Bert
Justyna Chmielewska
Sven Bergmann
Maximilian Busch
Wolfgang Driever
Karin Finger-Baier
Johanna Hößler
Almut Köhler
Nora Leich
Thomas Misgeld
Torsten Nöldner
Annegret Reiher
Manfred Schartl
Anja Seebach-Sproedt
Thomas Thumberger
Gilbert Schönfelder
Barbara Grune
eng
uncontrolled
Danio-rerio
eng
uncontrolled
Zebrafish
eng
uncontrolled
Pain
Physiologie und verwandte Themen
Medizin und Gesundheit
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/18878/Bert_TheEmboJournal_2016.pdf
21937
2020
eng
11
11
article
1
--
2020-11-20
--
Chromosome distribution of highly conserved tandemly arranged repetitive DNAs in the Siberian sturgeon (Acipenser baerii)
Polyploid genomes present a challenge for cytogenetic and genomic studies, due to the high number of similar size chromosomes and the simultaneous presence of hardly distinguishable paralogous elements. The karyotype of the Siberian sturgeon (Acipenser baerii) contains around 250 chromosomes and is remarkable for the presence of paralogs from two rounds of whole-genome duplications (WGD). In this study, we applied the sterlet-derived acipenserid satDNA-based whole chromosome-specific probes to analyze the Siberian sturgeon karyotype. We demonstrate that the last genome duplication event in the Siberian sturgeon was accompanied by the simultaneous expansion of several repetitive DNA families. Some of the repetitive probes serve as good cytogenetic markers distinguishing paralogous chromosomes and detecting ancestral syntenic regions, which underwent fusions and fissions. The tendency of minisatellite specificity for chromosome size groups previously observed in the sterlet genome is also visible in the Siberian sturgeon. We provide an initial physical chromosome map of the Siberian sturgeon genome supported by molecular markers. The application of these data will facilitate genomic studies in other recent polyploid sturgeon species.
Genes
2073-4425
10.3390/genes11111375
urn:nbn:de:bvb:20-opus-219371
2020-12-18T16:37:19+00:00
sword
swordwue
attachment; filename=deposit.zip
0daeb2ad2196c036cb2c77957349ec15
Genes (2020) 11:11, 1375. https://doi.org/10.3390/genes11111375
false
true
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Larisa S. Biltueva
Dmitry Yu. Prokopov
Svetlana A. Romanenko
Elena A. Interesova
Manfred Schartl
Vladimir A. Trifonov
eng
uncontrolled
Acipenser baerii
eng
uncontrolled
sturgeon karyotype
eng
uncontrolled
whole-genome duplication
eng
uncontrolled
paralogs
eng
uncontrolled
polyploidy
eng
uncontrolled
acipenserid minisatellite
eng
uncontrolled
satellite DNA
eng
uncontrolled
tandem repeats
Biowissenschaften; Biologie
open_access
Theodor-Boveri-Institut für Biowissenschaften
Import
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/21937/genes-11-01375-v2.pdf
17677
2018
eng
1430-1444
6
10
article
1
2019-02-18
--
--
A comparative view on sex differentiation and gametogenesis genes in lungfish and coelacanths
Gonadal sex differentiation and reproduction are the keys to the perpetuation of favorable gene combinations and positively selected traits. In vertebrates, several gonad development features that differentiate tetrapods and fishes are likely to be, at least in part, related to the water-to-land transition. The collection of information from basal sarcopterygians, coelacanths, and lungfishes, is crucial to improve our understanding of the molecular evolution of pathways involved in reproductive functions, since these organisms are generally regarded as “living fossils” and as the direct ancestors of tetrapods. Here, we report for the first time the characterization of >50 genes related to sex differentiation and gametogenesis in Latimeria menadoensis and Protopterus annectens. Although the expression profiles of most genes is consistent with the intermediate position of basal sarcopterygians between actinopterygian fish and tetrapods, their phylogenetic placement and presence/absence patterns often reveal a closer affinity to the tetrapod orthologs. On the other hand, particular genes, for example, the male gonad factor gsdf (Gonadal Soma-Derived Factor), provide examples of ancestral traits shared with actinopterygians, which disappeared in the tetrapod lineage.
Genome Biology and Evolution
10.1093/gbe/evy101
urn:nbn:de:bvb:20-opus-176774
Genome Biology and Evolution 2018, 10(6):1430-1444. DOI: 10.1093/gbe/evy101
false
true
CC BY-NC: Creative-Commons-Lizenz: Namensnennung, Nicht kommerziell 4.0 International
Maria Assunta Biscotti
Mateus Contar Adolfi
Marco Barucca
Mariko Forconi
Alberto Pallavicini
Marco Gerdol
Adriana Canapa
Manfred Schartl
eng
uncontrolled
sex differentiation
eng
uncontrolled
Latimeria menadoensis
eng
uncontrolled
Protopterus annectens
eng
uncontrolled
evolution
eng
uncontrolled
testis
eng
uncontrolled
gametogenesis
eng
uncontrolled
ovary
Physiologie und verwandte Themen
open_access
Theodor-Boveri-Institut für Biowissenschaften
Förderzeitraum 2018
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/17677/Biscotti_Genome_Biology_and_Evolution.pdf
22732
2020
eng
10
article
1
2021-02-25
--
--
The transcriptome of the newt Cynops orientalis provides new insights into evolution and function of sexual gene networks in sarcopterygians
Amphibians evolved in the Devonian period about 400 Mya and represent a transition step in tetrapod evolution. Among amphibians, high-throughput sequencing data are very limited for Caudata, due to their largest genome sizes among terrestrial vertebrates. In this paper we present the transcriptome from the fire bellied newt Cynops orientalis. Data here presented display a high level of completeness, comparable to the fully sequenced genomes available from other amphibians. Moreover, this work focused on genes involved in gametogenesis and sexual development. Surprisingly, the gsdf gene was identified for the first time in a tetrapod species, so far known only from bony fish and basal sarcopterygians. Our analysis failed to isolate fgf24 and foxl3, supporting the possible loss of both genes in the common ancestor of Rhipidistians. In Cynops, the expression analysis of genes described to be sex-related in vertebrates singled out an expected functional role for some genes, while others displayed an unforeseen behavior, confirming the high variability of the sex-related pathway in vertebrates.
Scientific Reports
10.1038/s41598-020-62408-x
urn:nbn:de:bvb:20-opus-227326
publish
Scientific Reports (2020) 10:5445. https://doi.org/10.1038/s41598-020-62408-x
true
true
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Maria Assunta Biscotti
Federica Carducci
Marco Barucca
Marco Gerdol
Alberto Pallavicini
Manfred Schartl
Adriana Canapa
Mateus Contar Adolfi
eng
uncontrolled
developmental biology
eng
uncontrolled
evolution
Medizin und Gesundheit
open_access
Theodor-Boveri-Institut für Biowissenschaften
Förderzeitraum 2020
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/22732/Biscotti_s41598-020-62408-x.pdf
16775
2016
eng
21571
6
article
1
2018-08-30
--
--
The Lungfish Transcriptome: A Glimpse into Molecular Evolution Events at the Transition from Water to Land
Lungfish and coelacanths are the only living sarcopterygian fish. The phylogenetic relationship of lungfish to the last common ancestor of tetrapods and their close morphological similarity to their fossil ancestors make this species uniquely interesting. However their genome size, the largest among vertebrates, is hampering the generation of a whole genome sequence. To provide a partial solution to the problem, a high-coverage lungfish reference transcriptome was generated and assembled. The present findings indicate that lungfish, not coelacanths, are the closest relatives to land-adapted vertebrates. Whereas protein-coding genes evolve at a very slow rate, possibly reflecting a “living fossil” status, transposable elements appear to be active and show high diversity, suggesting a role for them in the remarkable expansion of the lungfish genome. Analyses of single genes and gene families documented changes connected to the water to land transition and demonstrated the value of the lungfish reference transcriptome for comparative studies of vertebrate evolution.
Scientific Reports
10.1038/srep21571
urn:nbn:de:bvb:20-opus-167753
Scientific Reports 6:21571 (2016). DOI: 10.1038/srep21571
false
true
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Maria Assunta Biscotti
Marco Gerdol
Adriana Canapa
Mariko Forconi
Ettore Olmo
Alberto Pallavicini
Marco Barucca
Manfred Schartl
eng
uncontrolled
lungfish
eng
uncontrolled
transcriptome
eng
uncontrolled
genome
eng
uncontrolled
sarcopterygian fish
Biowissenschaften; Biologie
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/16775/Biscotti_Scientific_Reports.pdf
14698
2016
eng
1600-1613
15
8
article
1
2017-04-07
--
--
Whole Genome Duplications Shaped the Receptor Tyrosine Kinase Repertoire of Jawed Vertebrates
The receptor tyrosine kinase (RTK) gene family, involved primarily in cell growth and differentiation, comprises proteins with a common enzymatic tyrosine kinase intracellular domain adjacent to a transmembrane region. The amino-terminal portion of RTKs is extracellular and made of different domains, the combination of which characterizes each of the 20 RTK subfamilies among mammals. We analyzed a total of 7,376 RTK sequences among 143 vertebrate species to provide here the first comprehensive census of the jawed vertebrate repertoire. We ascertained the 58 genes previously described in the human and mouse genomes and established their phylogenetic relationships. We also identified five additional RTKs amounting to a total of 63 genes in jawed vertebrates. We found that the vertebrate RTK gene family has been shaped by the two successive rounds of whole genome duplications (WGD) called 1R and 2R (1R/2R) that occurred at the base of the vertebrates. In addition, the Vegfr and Ephrin receptor subfamilies were expanded by single gene duplications. In teleost fish, 23 additional RTK genes have been retained after another expansion through the fish-specific third round (3R) of WGD. Several lineage-specific gene losses were observed. For instance, birds have lost three RTKs, and different genes are missing in several fish sublineages. The RTK gene family presents an unusual high gene retention rate from the vertebrate WGDs (58.75% after 1R/2R, 64.4% after 3R), resulting in an expansion that might be correlated with the evolution of complexity of vertebrate cellular communication and intracellular signaling.
Genome Biology Evolution
10.1093/gbe/evw103
urn:nbn:de:bvb:20-opus-146988
Genome Biology Evolution 8(5):1600–1613. doi:10.1093/gbe/evw103
Frédéric G. Brunet
Jean-Nicolas Volff
Manfred Schartl
eng
uncontrolled
receptor tyrosine kinase
eng
uncontrolled
vertebrates
eng
uncontrolled
deuterostomes
eng
uncontrolled
whole genome duplications
Medizin und Gesundheit
open_access
Theodor-Boveri-Institut für Biowissenschaften
Förderzeitraum 2016
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/14698/093_Brunet_evw103.pdf
6976
1993
eng
article
1
2013-08-23
--
--
Effect of growth hormone on the growth rate of the gilthead seabream (Sparus aurata), and use of different constructs for the production of transgenic fish
When bovine or human growth hormones (GH) were injected into 6 months old (about 10 g) gilthead seabream, the growth rate of the fish, as measured by changes in their weight, increased by only about 15% compared with the saline-injected control. No effect or even slight inhibition of the growth rate was obtained when chicken or porcine GHs were injected. In a preliminary experiment, it was found that injection ofthe native GH increased the growth rate ofthe fish by about 20% after treatment for only 2 weeks. An expression vector, using the pRE1 plasmid and transformation into MZl cells, produced the gilthead seabream GH, providing a supply for further experiments on the effect of the homologaus GH on growth. Two reporter genes, ß-galactosidase (lacZ) and melanoma oncogene of Xiphophorus (mrk YY), were microinjected into fertilized eggs of S. aurata. Expression of these two genes could be demonstrated in 2-day-old embryos, the lacZ gene by staining of its enzymatic product, and the mrk YY gene by its phenotypic expression.
8053
urn:nbn:de:bvb:20-opus-69765
In: Aquaculture, 1993, 111, S. 189-197
Deutsches Urheberrecht
Benzion Cavari
Bruria Funkenstein
Thomas T. Chen
Lucia Irene Gonzalez-Villasenor
Manfred Schartl
deu
swd
Goldbrasse
deu
swd
Somatotropin
deu
swd
Wachstum
deu
swd
Transgene Tiere
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/6976/Schartl_6976.pdf
5321
1993
eng
article
1
2011-12-07
--
--
All-fish gene constructs for growth hormone gene transfer in fish
In order to develop all-fish expression vectors for microinjection into fertilized fish eggs, we have prepared the following cunstructs: rainbow trout metallothionein a/b and the gilthead seabream growth hormone cDNA (ptMTa-gbsGHcDNA, ptMTb-gsbGHcDNA), carp ß-actin gilthead seabream GH cDNA (pcAßgsbGHcDNA). The inducible metallothionein promoters a and b were cloned from rainbow trout, and the constitutive promoter ß-actin was isolated from carp. The metallothionein promoters were cloned by using the PCR technique. The tMTa contains 430 bp, while the tMTb contains 260 bp (Hong et al. 1992). These two promoters were introduced to pGEM-3Z containing the GH cDNA of Sparus aurata to form ptMTa-gsbGH and ptMTb-gsbGH, respectively. The carp cytoplasmic ß-actin gene was chosen as a source for isolating strong constitutive regulatory sequences. One of these regulatory sequences in pUC118 was Iigated to GH cDNA of S. aurata to form the pcAß-gsbGHcDNA. Expression of the constructs containing the metallothionein promoters was tested in fish cell culture and was found tobe induced effectively by zinc. The ptMTa gsb-GH cDNA construct was microinjected into fertilized carp eggs, and integration in the genome of carp was detected in the DNA isolated from fins at the age of two months.
urn:nbn:de:bvb:20-opus-61612
6161
In: Fish Physiology and Biochemistry (1993) , 11, 345-352
Deutsches Urheberrecht
Benzion Cavari
Yunhan Hong
Bruria Funkenstein
Boaz Moav
Manfred Schartl
deu
swd
Physiologische Chemie
eng
uncontrolled
growth hormone gene
eng
uncontrolled
all-fish genes
eng
uncontrolled
transgenic fish
eng
uncontrolled
cellline transfection
eng
uncontrolled
Sparus aurata
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/5321/Schartl09.pdf
5375
1990
eng
article
1
2011-12-12
--
--
Oncofetal antigen in Xiphophorus detected by monoclonal antibodies directed against melanoma-associated antigens
Monoclonal antlbodies (MAbs) directed against Xiphophorus melanoma cells were deve(oped and tested by lndirect immunofluorescence and Immunoperoxidase staining for reactivity with a panel of I 5 allogeneic tissues and 12 allogeneic cell llnes. The reactivity of such MAbs was restricted to melanoma cells from tumor biopsies and melanoma-derived cell lines. ln addition, all embryonie cells of all histiotypes from developmental stages later than mld·organogenesis and from corresponding short term in vitro cultures reacted with these MAbs. ln contrast, normal tissues and organs from adult fish dlsplayed no reactivity, thus implying that the melanoma-associated antigens detected by the MAbs described are oncofetal antigens.
urn:nbn:de:bvb:20-opus-61784
6178
In: International journal of cancer (1990 ) , 45, 136-142
Deutsches Urheberrecht
Gerd Clauss
Christoph Winkler
Jürgen Lohmeyer
Fritz Anders
Manfred Schartl
deu
swd
Physiologische Chemie
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/5375/Schartl36.pdf
20251
2019
eng
42
10
article
1
2020-04-01
--
--
Sex and the TEs: transposable elements in sexual development and function in animals
Transposable elements are endogenous DNA sequences able to integrate into and multiply within genomes. They constitute a major source of genetic innovations, as they can not only rearrange genomes but also spread ready-to-use regulatory sequences able to modify host gene expression, and even can give birth to new host genes. As their evolutionary success depends on their vertical transmission, transposable elements are intrinsically linked to reproduction. In organisms with sexual reproduction, this implies that transposable elements have to manifest their transpositional activity in germ cells or their progenitors. The control of sexual development and function can be very versatile, and several studies have demonstrated the implication of transposable elements in the evolution of sex. In this review, we report the functional and evolutionary relationships between transposable elements and sexual reproduction in animals. In particular, we highlight how transposable elements can influence expression of sexual development genes, and how, reciprocally, they are tightly controlled in gonads. We also review how transposable elements contribute to the organization, expression and evolution of sexual development genes and sex chromosomes. This underscores the intricate co-evolution between host functions and transposable elements, which regularly shift from a parasitic to a domesticated status useful to the host.
Mobile DNA
10.1186/s13100-019-0185-0
urn:nbn:de:bvb:20-opus-202510
Mobile DNA (2019) 10:42. https://doi.org/10.1186/s13100-019-0185-0
false
true
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Corentin Dechaud
Jean-Nicolas Volff
Manfred Schartl
Magali Naville
eng
uncontrolled
Transposable element
eng
uncontrolled
Sex determination
eng
uncontrolled
Sexual development and function
eng
uncontrolled
Germline
eng
uncontrolled
piRNA
eng
uncontrolled
Sex chromosome
Biowissenschaften; Biologie
open_access
Theodor-Boveri-Institut für Biowissenschaften
Förderzeitraum 2019
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/20251/Dechaud_MobileDNA_2019.pdf
32512
2022
eng
443-457
4
30
article
1
--
--
--
Achiasmatic meiosis in the unisexual Amazon molly, Poecilia formosa
Unisexual reproduction, which generates clonal offspring, is an alternative strategy to sexual breeding and occurs even in vertebrates. A wide range of non-sexual reproductive modes have been described, and one of the least understood questions is how such pathways emerged and how they mechanistically proceed. The Amazon molly, Poecilia formosa, needs sperm from males of related species to trigger the parthenogenetic development of diploid eggs. However, the mechanism, of how the unreduced female gametes are produced, remains unclear. Cytological analyses revealed that the chromosomes of primary oocytes initiate pachytene but do not proceed to bivalent formation and meiotic crossovers. Comparing ovary transcriptomes of P. formosa and its sexual parental species revealed expression levels of meiosis-specific genes deviating from P. mexicana but not from P. latipinna. Furthermore, several meiosis genes show biased expression towards one of the two alleles from the parental genomes. We infer from our data that in the Amazon molly diploid oocytes are generated by apomixis due to a failure in the synapsis of homologous chromosomes. The fact that this failure is not reflected in the differential expression of known meiosis genes suggests the underlying molecular mechanism may be dysregulation on the protein level or misexpression of a so far unknown meiosis gene, and/or hybrid dysgenesis because of compromised interaction of proteins from diverged genomes.
Chromosome Research
10.1007/s10577-022-09708-2
urn:nbn:de:bvb:20-opus-325128
@articleDedukh.2022, author = Dedukh, Dmitrij and Da Cruz, Irene and Kneitz, Susanne and Marta, Anatolie and Ormanns, Jenny and Tichopád, Tomáš and Lu, Yuan and Alsheimer, Manfred and Janko, Karel and Schartl, Manfred, year = 2022, title = Achiasmatic meiosis in the unisexual Amazon molly, Poecilia formosa, pages = 443–457, volume = 30, number = 4, journal = Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, doi = 10.1007/s10577-022-09708-2
md5:81bd9c1a32d50a8b85c7fde8a47bf4de
2023-08-12T11:06:37+00:00
/tmp/phpREw4zV
bibtex
64d767bd11f318.48881201
Chromosome Research (2022) 30:4, 443-457. DOI: 10.1007/s10577-022-09708-2
false
true
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Dmitrij Dedukh
Irene Da Cruz
Susanne Kneitz
Anatolie Marta
Jenny Ormanns
Tomáš Tichopád
Yuan Lu
Manfred Alsheimer
Karel Janko
Manfred Schartl
eng
uncontrolled
meiosis
eng
uncontrolled
parthenogenesis
eng
uncontrolled
synaptonemal complex
eng
uncontrolled
recombination
eng
uncontrolled
crossing-over
eng
uncontrolled
achiasmatic
eng
uncontrolled
transcriptome
eng
uncontrolled
oogenesis
Medizin und Gesundheit
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/32512/s10577-022-09708-2.pdf
5373
1990
eng
article
1
2011-12-12
--
--
Taql and Mspl RFLPs are detected by the human 2,3-biphosphoglycerate mutase (BPGM) cDNA
No abstract available
urn:nbn:de:bvb:20-opus-61763
6176
In: Nucleic Acids Research (1990 ) , 18, 1928
Deutsches Urheberrecht
Nicholas C. Dracopoli
David M. Feltquate
Brigitta Sam
Manfred Schartl
deu
swd
Physiologische Chemie
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/5373/Schartl33.pdf
20133
2019
eng
5293
9
article
1
2020-03-12
--
--
The genome of the arapaima (Arapaima gigas) provides insights into gigantism, fast growth and chromosomal sex determination system
We have sequenced the genome of the largest freshwater fish species of the world, the arapaima. Analysis of gene family dynamics and signatures of positive selection identified genes involved in the specific adaptations and unique features of this iconic species, in particular it’s large size and fast growth. Genome sequences from both sexes combined with RAD-tag analyses from other males and females led to the isolation of male-specific scaffolds and supports an XY sex determination system in arapaima. Whole transcriptome sequencing showed that the product of the gland-like secretory organ on the head surface of males and females may not only provide nutritional fluid for sex-unbiased parental care, but that the organ itself has a more specific function in males, which engage more in parental care.
Scientific Reports
10.1038/s41598-019-41457-x
urn:nbn:de:bvb:20-opus-201333
Scientific Reports (2019) 9:5293. https://doi.org/10.1038/s41598-019-41457-x
false
true
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Kang Du
Sven Wuertz
Mateus Adolfi
Susanne Kneitz
Matthias Stöck
Marcos Oliveira
Rafael Nóbrega
Jenny Ormanns
Werner Kloas
Romain Feron
Christophe Klopp
Hugues Parrinello
Laurent Journot
Shunping He
John Postlethwait
Axel Meyer
Yann Guiguen
Manfred Schartl
eng
uncontrolled
Genome
deu
uncontrolled
Genomics
Medizin und Gesundheit
open_access
Theodor-Boveri-Institut für Biowissenschaften
Förderzeitraum 2019
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/20133/Du_ScientificReports_2019.pdf.pdf
7265
1991
eng
bookpart
1
2013-09-03
--
--
Oligonucleotide fingerprinting using simple repeat motifs: a convenient, ubiquitously applicable method to detect hypervariability for multiple purposes
A panel of simple repetitive oligonucleotide probes has been designed and tested for multilocus DNA fingerprinting in some 200 fungal, plant and animal species as well as man. To date at least one of the probes has been found to be informative in each species. The human genome, however, has been the major target of many fingerprintins studies. Using the probe (CAC)5 or (GTG)5, individualization of all humans is possible except for monozygotic twins. Paternity analyses are now perfonned on a routine basis by the use of multilocus fingerprints, inctuding also cases of deficiency, i.e. where one of the parents is not available for analysis. In forensie science stain analysis is feasible in all tissue remains containing nuc)eated cells. Depending on the degree of DNA degradation a variety of oligonucleotides are informative, and they have been proven useful in actual case work. Advantages in comparison to other methods including enzymatic DNA amplification techniques (PCR) are evident. Fingerprint patterns of tumors may be changed due to the gain or loss of chromosomes and/or intrachromosomal deletion and amplification events. Locus-specific probes were isolated from the human (CAC)5/( GTG)5 fingerprint with a varying degree of informativeness (monomorphic versus truly hypervariable markers). The feasibility of three different approaches. for the isolation of hypervariable mono-locus probes was evaluated. Finally, one particular mixed simple (gt)n(ga)m repeat locus in the second intron of the HLA-DRB genes has been scrutinized to allow comparison of the extent of exon-encoded (protein-) polymorphisms versus intronie bypervariability of simple repeats: adjacent to a single gene sequence (e.g. HLA-DRB1*0401) many different length alleles were found. Group-specific structures of basic repeats were identified within the evolutionarily related DRB alleles. As a further application it is suggested here that due to the ubiquitous interspersion of their targets, short probes for simple repeat sequences are especially useful tools for ordering genomic cosmid, yeast artificial chromosome and phage banks.
8066
urn:nbn:de:bvb:20-opus-86371
In: DNA Fingerprinting: Approaches and Applications / ed. Terry Burke. - Basel [u.a.]: Birkhäuser, 1991. - S. 50-69. - (Experientia : Supplementum ; 58). - ISBN 3-7643-2562-3
Deutsches Urheberrecht
J. T. Epplen
H. Ammer
C. Epplen
C. Kammerbauer
R. Mitreiter
L. Roewer
W. Schwaiger
V. Steimle
H. Zischler
E. Albert
A. Andreas
B. Beyermann
W. Meyer
J. Buitkamp
I. Nanda
P. Nürnberg
S. D. J. Pena
H. Pöche
W. Sprecher
Manfred Schartl
K. Weising
A. Yassouridis
deu
swd
DNS
deu
swd
Fingerprint-Verfahren
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/7265/Schartl_7265.pdf
5314
1994
eng
article
1
2011-12-01
--
--
Male polymorphism in Limia perugiae (Pisces: Poeciliidae)
The male-polymorphic poeciliid fish, Limia perugiae, a small teleostean endemic to the southeast of the Caribbean island Hispafiola, consists of three male size morphs with uniform females. Large males differentiate at a size va:rying between 25 and 38 mm; intermediate males, between 21 and 25 mm. Under competition, !arge males exhibit an elaborate courtship display, whereas small males show only a sneak-chase behavior. Intermediate males adapt their tactics to the respective competitors. However, all malemorphs can switch from courtship display to sneak-chase behavior. In large mating groups with four males of different size and five or six virgin females, large dominant a-males as weil as small subordinate \(\delta\)-males did not produce any offspring. Unexpectedly, all progeny were sired exclusively by the intemediate subordinate ß- and \(\gamma\)-males. Breeding experiments with the three male morphs can best be explained by a model of Y -linked genes for small and !arge size which are both suspended by the activity of an autosomal recessive repressor responsible for the development of intermediate males. The dominant allele of the recessive repressor, in either its homoorits heterozygous state, activates the Y-chromosomal genes for !arge or small size, respectively. Accordingly, intermediate males may produce male offspring of all size classes, depending on the presence of either the Y-linked gene or the autosomal repressor.
urn:nbn:de:bvb:20-opus-61573
6157
Behavior Genetics (1994) , 24, 95-101
Deutsches Urheberrecht
Claudia Erbelding-Denk
Johannes H. Schroder
Manfred Schartl
Indrajit Nanda
Michael Schmid
Jörg T. Epplen
deu
swd
Physiologische Chemie
eng
uncontrolled
Poeciliid fish
eng
uncontrolled
male size polymorphism
eng
uncontrolled
reproductive success
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/5314/Schartl04.pdf
7269
1987
eng
article
1
2013-09-03
--
--
Karyotype and isozyme patterns of five species of Aulonocara REGAN, 1922
No abstract available.
8070
urn:nbn:de:bvb:20-opus-86774
In: Courier Forschungsinstitut Senckenberg, 1987, 94, S. 55-61
Deutsches Urheberrecht
Wolfgang Foerster
Manfred Schartl
deu
swd
Aulonocara
deu
swd
Karyotyp
deu
swd
Isoenzym
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/7269/Schartl_7269.pdf
28523
2020
eng
24
21
article
1
--
2020-12-15
--
Evolution of microRNA biogenesis genes in the sterlet (Acipenser ruthenus) and other polyploid vertebrates
MicroRNAs play a crucial role in eukaryotic gene regulation. For a long time, only little was known about microRNA-based gene regulatory mechanisms in polyploid animal genomes due to difficulties of polyploid genome assembly. However, in recent years, several polyploid genomes of fish, amphibian, and even invertebrate species have been sequenced and assembled. Here we investigated several key microRNA-associated genes in the recently sequenced sterlet (Acipenser ruthenus) genome, whose lineage has undergone a whole genome duplication around 180 MYA. We show that two paralogs of drosha, dgcr8, xpo1, and xpo5 as well as most ago genes have been retained after the acipenserid-specific whole genome duplication, while ago1 and ago3 genes have lost one paralog. While most diploid vertebrates possess only a single copy of dicer1, we strikingly found four paralogs of this gene in the sterlet genome, derived from a tandem segmental duplication that occurred prior to the last whole genome duplication. ago1,3,4 and exportins1,5 look to be prone to additional segment duplications producing up to four-five paralog copies in ray-finned fishes. We demonstrate for the first time exon microsatellite amplification in the acipenserid drosha2 gene, resulting in a highly variable protein product, which may indicate sub- or neofunctionalization. Paralogous copies of most microRNA metabolism genes exhibit different expression profiles in various tissues and remain functional despite the rediploidization process. Subfunctionalization of microRNA processing gene paralogs may be beneficial for different pathways of microRNA metabolism. Genetic variability of microRNA processing genes may represent a substrate for natural selection, and, by increasing genetic plasticity, could facilitate adaptations to changing environments.
International Journal of Molecular Sciences
1422-0067
10.3390/ijms21249562
urn:nbn:de:bvb:20-opus-285230
2022-09-05T21:38:29+00:00
sword
swordwue
attachment; filename=deposit.zip
f5777def8e8de7ca427cb28f265f74df
International Journal of Molecular Sciences (2020) 21:24, 9562. https://doi.org/10.3390/ijms21249562
false
true
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Mikhail V. Fofanov
Dmitry Yu. Prokopov
Heiner Kuhl
Manfred Schartl
Vladimir A. Trifonov
eng
uncontrolled
sturgeon
eng
uncontrolled
whole genome duplication
eng
uncontrolled
microRNA
eng
uncontrolled
gene duplications
Biowissenschaften; Biologie
open_access
Theodor-Boveri-Institut für Biowissenschaften
Import
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/28523/ijms-21-09562-v3.pdf
13099
2013
eng
e56006
4
8
article
1
2016-03-31
--
--
Characterization of Sex Determination and Sex Differentiation Genes in Latimeria
Genes involved in sex determination and differentiation have been identified in mice, humans, chickens, reptiles, amphibians and teleost fishes. However, little is known of their functional conservation, and it is unclear whether there is a common set of genes shared by all vertebrates. Coelacanths, basal Sarcopterygians and unique "living fossils", could help establish an inventory of the ancestral genes involved in these important developmental processes and provide insights into their components. In this study 33 genes from the genome of Latimeria chalumnae and from the liver and testis transcriptomes of Latimeria menadoensis, implicated in sex determination and differentiation, were identified and characterized and their expression levels measured. Interesting findings were obtained for GSDF, previously identified only in teleosts and now characterized for the first time in the sarcopterygian lineage; FGF9, which is not found in teleosts; and DMRT1, whose expression in adult gonads has recently been related to maintenance of sexual identity. The gene repertoire and testis-specific gene expression documented in coelacanths demonstrate a greater similarity to modern fishes and point to unexpected changes in the gene regulatory network governing sexual development.
PLoS ONE
10.1371/journal.pone.0056006
urn:nbn:de:bvb:20-opus-130995
PLoS ONE 8(4): e56006. doi:10.1371/journal.pone.0056006
Mariko Forconi
Adriana Canapa
Marco Barucca
Maria A. Biscotti
Teresa Capriglione
Francesco Buonocore
Anna M. Fausto
Daisy M. Makapedua
Alberto Pallavicini
Marco Gerdol
Gianluca De Moro
Giuseppe Scapigliati
Ettore Olmo
Manfred Schartl
eng
uncontrolled
medaka fish
eng
uncontrolled
mullerian hormone AMH
eng
uncontrolled
DM-domain gene
eng
uncontrolled
oryzias latipes
eng
uncontrolled
monodelphis domestica
eng
uncontrolled
oreochromis niloticus
eng
uncontrolled
dimorphic expression
eng
uncontrolled
molecular mechanisms
eng
uncontrolled
genomic organization
eng
uncontrolled
regulatory regions
Medizin und Gesundheit
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/13099/086_Forconi_Plos_One.pdf
5374
1990
eng
article
1
2011-12-12
--
--
Transient expression directed by homologous and heterologous promoter and enhancer sequences in fish cells
ln order to construct fish specific expression vectors for studies on gene regulation in vitro and in vivo a variety of heterologous enhancers and promoters from mammals and from viruses of higher vertebrate cells were tested for expression of the bacterial chloramphenicol acetyl transferase reporter gene in three teleost fish cell lines. Several viral enhancers were found to be constitutively active at high Ieveis. The human metallothionein promoter showed inducible expression in the presence of heavy metal Ions. A fish sequence was isolated that can be used as a homologous constitutively active promoter for expression of foreign genes. Using the human growth hormone gene with an active promoter in fish cells for transient expression insufficient splicing and Iack of translation were observed, pointing to limitations in the use of heterologous genes in gene transfer experiments. On the contrary, some heterologous promoters and enhancers functioned in fish c as weil as in their cell type of origin, indicating t at corresponding transcription factors are sufficient conserved between fish and human over a period of 900 million years of Independent evolution.
urn:nbn:de:bvb:20-opus-61774
6177
In: Nucleic Acids Research (1990) 18, 3299-3305
Deutsches Urheberrecht
Hildegard Friedenreich
Manfred Schartl
deu
swd
Physiologische Chemie
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/5374/Schartl34.pdf
11600
2014
eng
e100250
6
9
article
1
2015-07-16
--
--
Novel Method for Analysis of Allele Specific Expression in Triploid Oryzias latipes Reveals Consistent Pattern of Allele Exclusion
Assessing allele-specific gene expression (ASE) on a large scale continues to be a technically challenging problem. Certain biological phenomena, such as X chromosome inactivation and parental imprinting, affect ASE most drastically by completely shutting down the expression of a whole set of alleles. Other more subtle effects on ASE are likely to be much more complex and dependent on the genetic environment and are perhaps more important to understand since they may be responsible for a significant amount of biological diversity. Tools to assess ASE in a diploid biological system are becoming more reliable. Non-diploid systems are, however, not uncommon. In humans full or partial polyploid states are regularly found in both healthy (meiotic cells, polynucleated cell types) and diseased tissues (trisomies, non-disjunction events, cancerous tissues). In this work we have studied ASE in the medaka fish model system. We have developed a method for determining ASE in polyploid organisms from RNAseq data and we have implemented this method in a software tool set. As a biological model system we have used nuclear transplantation to experimentally produce artificial triploid medaka composed of three different haplomes. We measured ASE in RNA isolated from the livers of two adult, triploid medaka fish that showed a high degree of similarity. The majority of genes examined (82%) shared expression more or less evenly among the three alleles in both triploids. The rest of the genes (18%) displayed a wide range of ASE levels. Interestingly the majority of genes (78%) displayed generally consistent ASE levels in both triploid individuals. A large contingent of these genes had the same allele entirely suppressed in both triploids. When viewed in a chromosomal context, it is revealed that these genes are from large sections of 4 chromosomes and may be indicative of some broad scale suppression of gene expression.
PLOS ONE
10.1371/journal.pone.0100250
1932-6203
24945156
urn:nbn:de:bvb:20-opus-116000
PLoS ONE 9(6): e100250. doi:10.1371/journal.pone.0100250
Tzintzuni I. Garcia
Isa Matos
Yingjia Shen
Vagmita Pabuwal
Maria Manuela Coelho
Yuko Wakamatsu
Manfred Schartl
Ronald B. Walter
eng
uncontrolled
RNA-SEQ data
eng
uncontrolled
copy-number alteration
eng
uncontrolled
squalius alburnoides
eng
uncontrolled
gene expression
eng
uncontrolled
medaka
eng
uncontrolled
variant detection
eng
uncontrolled
transplantation
eng
uncontrolled
genome
eng
uncontrolled
generation
eng
uncontrolled
evolution
Medizin und Gesundheit
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/11600/070_Garcia_PLOS_ONE.pdf
5954
1994
eng
conferenceobject
1
2012-06-28
--
--
The conservation of neurotrophic factors during vertebrate evolution
No abstract available
urn:nbn:de:bvb:20-opus-61964
6196
In: Comparative Biochemistry and Physiology / Part C: Pharmacology, Toxicology and Endocrinology (1994) 108, 1-10.
Deutsches Urheberrecht
R. Gotz
Manfred Schartl
deu
swd
Physiologische Chemie
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/5954/Schartl66.pdf
5286
1994
eng
article
1
2011-11-25
--
--
Neurotrophin-6 is a new member of the nerve growth factor family
DURING vertebrale development, many neurons depend for survival and differentiation on their target cells\(^{1-3}\). The best documented mediator of such a retrograde trophic action is the neurotrophin nerve growth factor (NGF)\(^1\). NGF and the other known members of tbe neurotrophin family, brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT -3) and neurotrophin-4/5 (NT -4/5) are conserved as distinct genes over large evolutionary distances\(^{4 -6}\). Here we report the cloning of neurotrophin-6 (NT -6), a new member of this family from the teleost fish Xiphophorus. NT -6 distinguishes itself from the other known neurotrophins in that it is not found as a soluble protein in the medium of producing cells. The addition of heparin (but not chondroitin) effects the release of NT -6 from cell surface and extracellular matrix molecules. Recombinant purified NT -6 has a spectrum of actions similar to NGF on chick sympathetic and sensory neurons, albeit with a lower potency. NT -6 is expressed in tbe embryonie valvulla cerebelli; expression persists in some adult tissues. The interaction of NT-6 with heparin-binding molecuJes may modulate its action in the nervous system .
urn:nbn:de:bvb:20-opus-61544
6154
Nature (1994) , 372, 266-269
Deutsches Urheberrecht
Rudolf Götz
Reinhard Köster
Christoph Winkler
Friedrich Raulf
Friedrich Lottspeich
Manfred Schartl
Hans Thoenen
deu
swd
Physiologische Chemie
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/5286/Schartl01.pdf
7194
1991
eng
article
1
2013-08-22
--
--
Conservation of structure and expression of the c-yes and fyn genes in lower vertebrates
The src-gene family in mammals and birds consists of 9 closely related protein tyrosine kinases. We have cloned the c-yes and fyn bomologues of the src-family from the teleost fish Xiphophorus helleri. Both genes show a high degree of sequence conservation and exhibit all structural motifs diagnostic for functional src-like protein tyrosine kinases. Sequence comparisons revealed three domains (exon 2, exons 3--6, exons 7-12) which evolve at different rates. Both genes exhibit an identical expression pattern, with preferential expression in neural tissues. No transcripts of c-yes were found in liver wbich is contrary to the situation in higher vertebrales. In malignant melanoma, elevated Ieveis of c-yes andfyn were detected indicating a possible function during secondary steps of tumor progression for src-related tyrosine kinases.
8034
urn:nbn:de:bvb:20-opus-86723
In: Oncogene, 1991, 6, S. 361-369
Deutsches Urheberrecht
Gerhard Hannig
Sabine Ottilie
Manfred Schartl
deu
swd
Konservierung
deu
swd
Gen
deu
swd
Niedere Wirbeltiere
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/7194/Schartl_7194.pdf
12064
2014
eng
5040-53
13
5
article
1
2015-10-21
--
--
The MAPK pathway as an apoptosis enhancer in melanoma
Inhibition of RAF/MEK/ERK signaling is beneficial for many patients with BRAFV600E–mutated melanoma. However, primary and secondary resistances restrict long-lasting therapy success. Combination therapies are therefore urgently needed. Here, we evaluate the cellular effect of combining a MEK inhibitor with a genotoxic apoptosis inducer. Strikingly, we observed that an activated MAPK pathway promotes in several melanoma cell lines the pro-apoptotic response to genotoxic stress, and MEK inhibition reduces intrinsic apoptosis. This goes along with MEK inhibitor induced increased RAS and P-AKT levels. The protective effect of the MEK inhibitor depends on PI3K signaling, which prevents the induction of pro-apoptotic PUMA that mediates apoptosis after DNA damage. We could show that the MEK inhibitor dependent feedback loop is enabled by several factors, including EGF receptor and members of the SPRED family. The simultaneous knockdown of SPRED1 and SPRED2 mimicked the effects of MEK inhibitor such as PUMA repression and protection from apoptosis. Our data demonstrate that MEK inhibition of BRAFV600E-positive melanoma cells can protect from genotoxic stress, thereby achieving the opposite of the intended anti-tumorigenic effect of the combination of MEK inhibitor with inducers of intrinsic apoptosis.
Oncotarget
1949-2553
urn:nbn:de:bvb:20-opus-120649
Oncotarget, Vol. 5, No. 13, 5040-53
Johannes M. Haydn
Anita Hufnagel
Johannes Grimm
Katja Maurus
Manfred Schartl
Svenja Meierjohann
eng
uncontrolled
PI3K
eng
uncontrolled
melanoma
eng
uncontrolled
RAS
eng
uncontrolled
chemotherapy resistance
eng
uncontrolled
crosstalk
Pharmakologie, Therapeutik
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/12064/110_Hydn_Oncotarget.pdf
26061
2021
eng
10
16
article
1
2022-03-16
--
--
Differential expression of transposable elements in the medaka melanoma model
Malignant melanoma incidence is rising worldwide. Its treatment in an advanced state is difficult, and the prognosis of this severe disease is still very poor. One major source of these difficulties is the high rate of metastasis and increased genomic instability leading to a high mutation rate and the development of resistance against therapeutic approaches. Here we investigate as one source of genomic instability the contribution of activation of transposable elements (TEs) within the tumor. We used the well-established medaka melanoma model and RNA-sequencing to investigate the differential expression of TEs in wildtype and transgenic fish carrying melanoma. We constructed a medaka-specific TE sequence library and identified TE sequences that were specifically upregulated in tumors. Validation by qRT- PCR confirmed a specific upregulation of a LINE and an LTR element in malignant melanomas of transgenic fish.
PLoS One
10.1371/journal.pone.0251713
urn:nbn:de:bvb:20-opus-260615
publish
PLoS One (2021) 16:10, e0251713. https://doi.org/10.1371/journal.pone.0251713
false
true
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Frederik Helmprobst
Susanne Kneitz
Barbara Klotz
Magali Naville
Corentin Dechaud
Jean-Nicolas Volff
Manfred Schartl
eng
uncontrolled
melanoma
eng
uncontrolled
genomics
eng
uncontrolled
transposable elements
eng
uncontrolled
cancer genomics
eng
uncontrolled
malignant tumors
eng
uncontrolled
gene prediction
eng
uncontrolled
human genomics
eng
uncontrolled
retrotransposons
Medizin und Gesundheit
open_access
Theodor-Boveri-Institut für Biowissenschaften
Förderzeitraum 2021
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/26061/journal.pone.0251713.pdf
13226
2013
eng
2328-2346
10
30
article
1
2016-04-15
--
--
Divergent Expression Regulation of Gonad Development Genes in Medaka Shows Incomplete Conservation of the Downstream Regulatory Network of Vertebrate Sex Determination
Genetic control of male or female gonad development displays between different groups of organisms a remarkable diversity of “master sex-determining genes” at the top of the genetic hierarchies, whereas downstream components surprisingly appear to be evolutionarily more conserved. Without much further studies, conservation of sequence has been equalized to conservation of function. We have used the medaka fish to investigate the generality of this paradigm. In medaka, the master male sex-determining gene is dmrt1bY, a highly conserved downstream regulator of sex determination in vertebrates. To understand its function in orchestrating the complex gene regulatory network, we have identified targets genes and regulated pathways of Dmrt1bY. Monitoring gene expression and interactions by transgenic fluorescent reporter fish lines, in vivo tissue-chromatin immunoprecipitation and in vitro gene regulation assays revealed concordance but also major discrepancies between mammals and medaka, notably amongst spatial, temporal expression patterns and regulations of the canonical Hedgehog and R-spondin/Wnt/Follistatin signaling pathways. Examination of Foxl2 protein distribution in the medaka ovary defined a new subpopulation of theca cells, where ovarian-type aromatase transcriptional regulation appears to be independent of Foxl2. In summary, these data show that the regulation of the downstream regulatory network of sex determination is less conserved than previously thought.
Molecular Biology and Evolution
10.1093/molbev/mst130
urn:nbn:de:bvb:20-opus-132262
Molecular Biology and Evolution 30(10):2328–2346 doi:10.1093/molbev/mst130
222719
Amaury Herpin
Mateus C. Adolfi
Barbara Nicol
Maria Hinzmann
Cornelia Schmidt
Johanna Klughammer
Mareen Engel
Minoru Tanaka
Yann Guiguen
Manfred Schartl
eng
uncontrolled
gonadal development
eng
uncontrolled
divergent expression regulation
eng
uncontrolled
gene regulatory network evolution
eng
uncontrolled
adaptive evolution
Medizin und Gesundheit
open_access
Theodor-Boveri-Institut für Biowissenschaften
OpenAIRE
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/13226/156_Herpin_Molecular_Biology_and Evolution.pdf
5643
2010
eng
article
1
2012-03-02
--
--
Transcriptional Rewiring of the Sex Determining dmrt1 Gene Duplicate by Transposable Elements
Control and coordination of eukaryotic gene expression rely on transcriptional and posttranscriptional regulatory networks. Evolutionary innovations and adaptations often require rapid changes of such networks. It has long been hypothesized that transposable elements (TE) might contribute to the rewiring of regulatory interactions. More recently it emerged that TEs might bring in ready-to-use transcription factor binding sites to create alterations to the promoters by which they were captured. A process where the gene regulatory architecture is of remarkable plasticity is sex determination. While the more downstream components of the sex determination cascades are evolutionary conserved, the master regulators can switch between groups of organisms even on the interspecies level or between populations. In the medaka fish (Oryzias latipes) a duplicated copy of dmrt1, designated dmrt1bY or DMY, on the Y chromosome was shown to be the master regulator of male development, similar to Sry in mammals. We found that the dmrt1bY gene has acquired a new feedback downregulation of its expression. Additionally, the autosomal dmrt1a gene is also able to regulate transcription of its duplicated paralog by binding to a unique target Dmrt1 site nested within the dmrt1bY proximal promoter region. We could trace back this novel regulatory element to a highly conserved sequence within a new type of TE that inserted into the upstream region of dmrt1bY shortly after the duplication event. Our data provide functional evidence for a role of TEs in transcriptional network rewiring for sub- and/or neo-functionalization of duplicated genes. In the particular case of dmrt1bY, this contributed to create new hierarchies of sex-determining genes.
urn:nbn:de:bvb:20-opus-68437
6843
PLOS GENETICS (2010) 6, 2, DOI: 10.1371/journal.pgen.1000844
Amaury Herpin
Ingo Braasch
Michael Kraeussling
Cornelia Schmidt
Eva C. Thoma
Shuhei Nakamura
Minoru Tanaka
Manfred Schartl
deu
swd
Gen
eng
uncontrolled
dmrt1
eng
uncontrolled
sex-determining gene
Biowissenschaften; Biologie
open_access
Institut für Virologie und Immunbiologie
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/5643/Herpin_journal.pgen.1000844.pdf
22581
2021
eng
1
43
article
1
--
--
--
Skipping sex: A nonrecombinant genomic assemblage of complementary reproductive modules
The unusual occurrence and developmental diversity of asexual eukaryotes remain a puzzle. De novo formation of a functioning asexual genome requires a unique assembly of sets of genes or gene states to disrupt cellular mechanisms of meiosis and gametogenesis, and to affect discrete components of sexuality and produce clonal or hemiclonal offspring. We highlight two usually overlooked but essential conditions to understand the molecular nature of clonal organisms, that is, a nonrecombinant genomic assemblage retaining modifiers of the sexual program, and a complementation between altered reproductive components. These subtle conditions are the basis for physiologically viable and genetically balanced transitions between generations. Genomic and developmental evidence from asexual animals and plants indicates the lack of complementation of molecular changes in the sexual reproductive program is likely the main cause of asexuals' rarity, and can provide an explanatory frame for the developmental diversity and lability of developmental patterns in some asexuals as well as for the discordant time to extinction estimations.
BioEssays
10.1002/bies.202000111
urn:nbn:de:bvb:20-opus-225818
2021-02-22T14:30:39+00:00
sword
swordwue
attachment; filename=deposit.zip
dd3ce5f23f4fe3c2a2dea10b0ebe7bec
BioEssays 2021, 43(1):2000111. DOI: 10.1002/bies.202000111
false
true
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Diego Hojsgaard
Manfred Schartl
eng
uncontrolled
amphimixis
eng
uncontrolled
apomixis
eng
uncontrolled
automixis
eng
uncontrolled
gynogenesis
eng
uncontrolled
hybridogenesis
eng
uncontrolled
parthenogenesis
Biowissenschaften; Biologie
open_access
Theodor-Boveri-Institut für Biowissenschaften
Import
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/22581/BIES_BIES202000111.pdf
5336
1993
eng
article
1
2011-12-07
--
--
Sequence of the growth hormone (GH) gene from the silver carp (Hypophthalmichthys molitrix) and evolution of GH genes in vertebrates
The silver carp (Hypophthalmichthys molitrix) growth hormone (GH) genewas isolated and sequenced following amplification from genomic DNA by the polymerase chain reaction. The gene spans a region of approx. 2.5 kb nucleotides (nt) and consists of five exons. The sequence predicts a polypeptide of 210 amino acids (aa) including a putative signal peptide of 22 hydrophobic aa residues. The arrangement of exons and introns is identical to the GH genes of common carp, grass carp, and very similar to mammals and birds, but quite different from that for the GH genes of tilapia and salmonids. The silver carp GH gene shares a high homology at the nt and aa Ievels with those of grass carp (95.3% nt, 99.5% aa) and of common carp (81% nt, 95.7% aa).
urn:nbn:de:bvb:20-opus-61620
6162
In: Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression (1993) , 1174, 285-288
Deutsches Urheberrecht
Yunhan Hong
Manfred Schartl
deu
swd
Physiologische Chemie
eng
uncontrolled
Recombinant DNA ; Growth hormone gene ; PCR; Silver carp ; Fish
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/5336/Schartl10.pdf
5341
1992
eng
article
1
2011-12-07
--
--
Structure of the rainbow trout metallothionein A gene
To investigate the regulation of metallothionein-encoding genes in fish, we have isolated and sequenced the rainbow trout metallothionein-A-encoding gene (tMT-A) by polymerase chain reaction. This gene spans about 1.1 kb, consists of three exons and two introns, and has an A+ T-rieb 5' -region which contains a TATAAA signal, and two metal responsive elements (MREs). The transcription start point is centered around an A residue 81 nt upstream of the ATG codon.
urn:nbn:de:bvb:20-opus-61679
6167
In: Gene (1992) , 120, 277-279
Deutsches Urheberrecht
Yunhan Hong
Manfred Schartl
deu
swd
Physiologische Chemie
eng
uncontrolled
Recombinant DNA ; polymerase chain reaction ; metallothionein gene ; rainbow trout ; fish
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/5341/Schartl15.pdf
5340
1993
eng
article
1
2011-12-07
--
--
Development of a heavy metal-inducible fish-specific expression vector for gene transfer in vitro and in vivo
The promoter of the rainbow trout metallothionein B gene ( tMTb) was isolated from genomic DNA by the polymerase chain reaction (PCR ), fused to the bacterial chloramphenicol acetyltransferase (CAT) genein an expression vector, and functionally analyzed in one human cellline and four fish celllines. This promoter exhibited an extremely low basal expression in all celllines and was zincand cadmium-inducible except in the fish melanoma cell line where the promoter was completely inactive. The metal-induced expression patterns were cellline-specific. In general the fish promoter was more species- and cell type-specific than its human counterpart. In a transient assay it was functional in developing embryos of the medaka ( Oryzias /atipes). These properties make this promoter suitable for inducible, tissue-specific expression of transgenes and for in vivo studies of gene function and regulation.
urn:nbn:de:bvb:20-opus-61666
6166
In: Aquaculture (1993) , 111, 215-226
Deutsches Urheberrecht
Yunhan Hong
Christoph Winkler
Gottfried Brem
Manfred Schartl
deu
swd
Physiologische Chemie
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/5340/Schartl14.pdf
16495
2016
eng
7207-7220
20
6
article
1
2018-07-19
--
--
Evolution of the elaborate male intromittent organ of Xiphophorus fishes
Internally fertilizing animals show a remarkable diversity in male genital morphology that is associated with sexual selection, and these traits are thought to be evolving particularly rapidly. Male fish in some internally fertilizing species have “gonopodia,” highly modified anal fins that are putatively important for sexual selection. However, our understanding of the evolution of genital diversity remains incomplete. Contrary to the prediction that male genital traits evolve more rapidly than other traits, here we show that gonopodial traits and other nongonopodial traits exhibit similar evolutionary rates of trait change and also follow similar evolutionary models in an iconic genus of poeciliid fish (Xiphophorus spp.). Furthermore, we find that both mating and nonmating natural selection mechanisms are unlikely to be driving the diverse Xiphophorus gonopodial morphology. Putative holdfast features of the male genital organ do not appear to be influenced by water flow, a candidate selective force in aquatic habitats. Additionally, interspecific divergence in gonopodial morphology is not significantly higher between sympatric species, than between allopatric species, suggesting that male genitals have not undergone reproductive character displacement. Slower rates of evolution in gonopodial traits compared with a subset of putatively sexually selected nongenital traits suggest that different selection mechanisms may be acting on the different trait types. Further investigations of this elaborate trait are imperative to determine whether it is ultimately an important driver of speciation.
Ecology and Evolution
10.1002/ece3.2396
urn:nbn:de:bvb:20-opus-164956
Ecology and Evolution 2016; 6(20):7207-7220
false
true
Julia C. Jones
Carmelo Fruciano
Anja Keller
Manfred Schartl
Axel Meyer
eng
uncontrolled
Male intromittent organ
eng
uncontrolled
reproductive character displacement
eng
uncontrolled
sexual selection
eng
uncontrolled
species diversification
eng
uncontrolled
Xiphophorus fish
Biowissenschaften; Biologie
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/16495/037_Jones_ECOLOGY AND EVOLUTION.pdf
14413
2015
eng
848-864
4
5
article
1
2017-02-08
--
--
Transcriptomics of two evolutionary novelties: how to make a sperm-transfer organ out of an anal fin and a sexually selected "sword" out of a caudal fin
Swords are exaggerated male ornaments of swordtail fishes that have been of great interest to evolutionary biologists ever since Darwin described them in the Descent of Man (1871). They are a novel sexually selected trait derived from modified ventral caudal fin rays and are only found in the genus Xiphophorus. Another phylogenetically more widespread and older male trait is the gonopodium, an intromittent organ found in all poeciliid fishes, that is derived from a modified anal fin. Despite many evolutionary and behavioral studies on both traits, little is known so far about the molecular mechanisms underlying their development. By investigating transcriptomic changes (utilizing a RNA-Seq approach) in response to testosterone treatment in the swordtail fish, Xiphophorus hellerii, we aimed to better understand the architecture of the gene regulatory networks underpinning the development of these two evolutionary novelties. Large numbers of genes with tissue-specific expression patterns were identified. Among the sword genes those involved in embryonic organ development, sexual character development and coloration were highly expressed, while in the gonopodium rather more morphogenesis-related genes were found. Interestingly, many genes and genetic pathways are shared between both developing novel traits derived from median fins: the sword and the gonopodium. Our analyses show that a larger set of gene networks was co-opted during the development and evolution of the older gonopodium than in the younger, and morphologically less complex trait, the sword. We provide a catalog of candidate genes for future efforts to dissect the development of those sexually selected exaggerated male traits in swordtails.
Ecology and Evolution
10.1002/ece3.1390
urn:nbn:de:bvb:20-opus-144139
Ecology and Evolution 5:4, 848-864 (2015). DOI: 10.1002/ece3.1390
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Ji Hyoun Kang
Tereza Manousaki
Paolo Franchini
Susanne Kneitz
Manfred Schartl
Axel Meyer
eng
uncontrolled
mouse testis differentiation
eng
uncontrolled
fishes Xiphophorus
eng
uncontrolled
beetle horns
eng
uncontrolled
gonopodium
eng
uncontrolled
RNA-Seq
eng
uncontrolled
swordtails
eng
uncontrolled
Xiphophorus
eng
uncontrolled
key innovation
eng
uncontrolled
male-specific traits
eng
uncontrolled
Co-option
eng
uncontrolled
genus Xiphophorus
eng
uncontrolled
hybrid origin
eng
uncontrolled
Drosophila melanogaster
eng
uncontrolled
expression analysis
eng
uncontrolled
cell proliferation
eng
uncontrolled
preexisting bias
eng
uncontrolled
sex combs
Biowissenschaften; Biologie
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/14413/059_Kang_Ecology_and_Evolution.pdf
12185
2013
eng
25
13
article
1
2015-11-11
--
--
Comprehensive phylogenetic analysis of all species of swordtails and platies (Pisces: Genus Xiphophorus) uncovers a hybrid origin of a swordtail fish, Xiphophorus monticolus, and demonstrates that the sexually selected sword originated in the ancestral lineage of the genus, but was lost again secondarily
Background: Males in some species of the genus Xiphophorus, small freshwater fishes from Meso-America, have an extended caudal fin, or sword - hence their common name "swordtails". Longer swords are preferred by females from both sworded and - surprisingly also, non-sworded (platyfish) species that belong to the same genus. Swordtails have been studied widely as models in research on sexual selection. Specifically, the pre-existing bias hypothesis was interpreted to best explain the observed bias of females in presumed ancestral lineages of swordless species that show a preference for assumed derived males with swords over their conspecific swordless males. However, many of the phylogenetic relationships within this genus still remained unresolved. Here we construct a comprehensive molecular phylogeny of all 26 known Xiphophorus species, including the four recently described species (X. kallmani, X. mayae, X. mixei and X. monticolus). We use two mitochondrial and six new nuclear markers in an effort to increase the understanding of the evolutionary relationships among the species in this genus. Based on the phylogeny, the evolutionary history and character state evolution of the sword was reconstructed and found to have originated in the common ancestral lineage of the genus Xiphophorus and that it was lost again secondarily.
Results: We estimated the evolutionary relationships among all known species of the genus Xiphophorus based on the largest set of DNA markers so far. The phylogeny indicates that one of the newly described swordtail species, Xiphophorus monticolus, is likely to have arisen through hybridization since it is placed with the southern platyfish in the mitochondrial phylogeny, but with the southern swordtails in the nuclear phylogeny. Such discordance between these two types of markers is a strong indication for a hybrid origin. Additionally, by using a maximum likelihood approach the possession of the sexually selected sword trait is shown to be the most likely ancestral state for the genus Xiphophorus. Further, we provide a well supported estimation of the phylogenetic relationships between the previously unresolved northern swordtail groups.
Conclusions: This comprehensive molecular phylogeny of the entire genus Xiphophorus provides evidence that a second swordtail species, X. monticolus, arose through hybridization. Previously, we demonstrated that X. clemenciae, another southern swordtail species, arose via hybridization. These findings highlight the potential key role of hybridization in the evolution of this genus and suggest the need for further investigations into how hybridization contributes to speciation more generally.
BMC Evolutionary Biology
10.1186/1471-2148-13-25
1471-2148
urn:nbn:de:bvb:20-opus-121853
BMC Evolutionary Biology 2013, 13:25. doi:10.1186/1471-2148-13-25
Ji Hyoun Kang
Manfred Schartl
Ronald B. Walter
Axel Meyer
eng
uncontrolled
parten-offspring conflict
eng
uncontrolled
introgressive hybridization
eng
uncontrolled
mitochondrial DNA
eng
uncontrolled
molecular phylogeny
eng
uncontrolled
likelihood approach
eng
uncontrolled
tree selection
eng
uncontrolled
preexisting bias
eng
uncontrolled
adaptive radiation
eng
uncontrolled
evolution
eng
uncontrolled
poeciliidae
Einzelne physiologische Systeme bei Tieren
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/12185/005_Kang_BMC_Evolutionary_Biology.pdf
14602
2016
eng
357
17
article
1
2017-03-22
--
--
Germ cell and tumor associated piRNAs in the medaka and \(Xiphophorus\) melanoma models
Background
A growing number of studies report an abnormal expression of Piwi-interacting RNAs (piRNAs) and the piRNA processing enzyme Piwi in many cancers. Whether this finding is an epiphenomenon of the chaotic molecular biology of the fast dividing, neoplastically transformed cells or is functionally relevant to tumorigenesisis is difficult to discern at present. To better understand the role of piRNAs in cancer development small laboratory fish models can make a valuable contribution. However, little is known about piRNAs in somatic and neoplastic tissues of fish.
Results
To identify piRNA clusters that might be involved in melanoma pathogenesis, we use several transgenic lines of medaka, and platyfish/swordtail hybrids, which develop various types of melanoma. In these tumors Piwi, is expressed at different levels, depending on tumor type. To quantify piRNA levels, whole piRNA populations of testes and melanomas of different histotypes were sequenced. Because no reference piRNA cluster set for medaka or Xiphophorus was yet available we developed a software pipeline to detect piRNA clusters in our samples and clusters were selected that were enriched in one or more samples. We found several loci to be overexpressed or down-regulated in different melanoma subtypes as compared to hyperpigmented skin. Furthermore, cluster analysis revealed a clear distinction between testes, low-grade and high-grade malignant melanoma in medaka.
Conclusions
Our data imply that dysregulation of piRNA expression may be associated with development of melanoma. Our results also reinforce the importance of fish as a suitable model system to study the role of piRNAs in tumorigenesis.
BMC Genomics
10.1186/s12864-016-2697-z
urn:nbn:de:bvb:20-opus-146028
BMC Genomics (2016) 17:357 DOI 10.1186/s12864-016-2697-z
Susanne Kneitz
Rasmi R. Mishra
Domitille Chalopin
John Postlethwait
Wesley C. Warren
Ronald B. Walther
Manfred Schartl
eng
uncontrolled
small RNA-sequencing
eng
uncontrolled
melanoma
eng
uncontrolled
piRNA
eng
uncontrolled
fish model
Menschliche Anatomie, Zytologie, Histologie
open_access
Theodor-Boveri-Institut für Biowissenschaften
Förderzeitraum 2016
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/14602/026_Kneitz_art_10.1186_s12864-016-2697-z.pdf
17658
2018
eng
233
5
9
article
1
2019-02-13
--
--
The colorful sex chromosomes of teleost fish
Teleost fish provide some of the most intriguing examples of sexually dimorphic coloration, which is often advantageous for only one of the sexes. Mapping studies demonstrated that the genetic loci underlying such color patterns are frequently in tight linkage to the sex-determining locus of a species, ensuring sex-specific expression of the corresponding trait. Several genes affecting color synthesis and pigment cell development have been previously described, but the color loci on the sex chromosomes have mostly remained elusive as yet. Here, we summarize the current knowledge about the genetics of such color loci in teleosts, mainly from studies on poeciliids and cichlids. Further studies on these color loci will certainly provide important insights into the evolution of sex chromosomes.
Genes
10.3390/genes9050233
urn:nbn:de:bvb:20-opus-176587
Genes 2018, 9(5), 233. DOI: 10.3390/genes9050233
false
true
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Verena A. Kottler
Manfred Schartl
eng
uncontrolled
teleost fish
eng
uncontrolled
sex chromosomes
eng
uncontrolled
coloration
eng
uncontrolled
pigment pattern
eng
uncontrolled
sexual conflict
eng
uncontrolled
sexually antagonistic genes
Wechselwarme Wirbeltiere; Pisces (Fische)
open_access
Theodor-Boveri-Institut für Biowissenschaften
Förderzeitraum 2018
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/17658/Kottler_Genes.pdf
5826
2011
eng
article
1
2012-05-04
--
--
Highly Asynchronous and Asymmetric Cleavage Divisions Accompany Early Transcriptional Activity in Pre-Blastula Medaka Embryos
In the initial phase of development of fish embryos, a prominent and critical event is the midblastula transition (MBT). Before MBT cell cycle is rapid, highly synchronous and zygotic gene transcription is turned off. Only during MBT the cell cycle desynchronizes and transcription is activated. Multiple mechanisms, primarily the nucleocytoplasmic ratio, are supposed to control MBT activation. Unexpectedly, we find in the small teleost fish medaka (Oryzias latipes) that at very early stages, well before midblastula, cell division becomes asynchronous and cell volumes diverge. Furthermore, zygotic transcription is extensively activated already after the 64-cell stage. Thus, at least in medaka, the transition from maternal to zygotic transcription is uncoupled from the midblastula stage and not solely controlled by the nucleocytoplasmic ratio.
urn:nbn:de:bvb:20-opus-68906
6890
PLoS One (2011) 6:7, doi:10.1371/journal.pone.0021741
Michael Kraeussling
Toni Ulrich Wagner
Manfred Schartl
deu
swd
Fische
deu
swd
Embryo
Biowissenschaften; Biologie
open_access
Theodor-Boveri-Institut für Biowissenschaften
Förderzeitraum 2011
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/5826/Kraeussling_journal.pone.0021741.pdf
5616
2010
eng
article
1
2012-02-24
--
--
Lineage-specific co-evolution of the Egf receptor/ligand signaling system
Background: The epidermal growth factor receptor (Egfr) with its numerous ligands has fundamental roles in development, cell differentiation and physiology. Dysfunction of the receptor-ligand system contributes to many human malignancies. Consistent with such various tasks, the Egfr gene family has expanded during vertebrate evolution as a consequence of several rounds of whole genome duplication. Of particular interest is the effect of the fish-specific whole genome duplication (FSGD) on the ligand-receptor system, as it has supplied this largest group of vertebrates with additional opportunities for sub- and/or neofunctionalization in this signaling system. Results: We identified the predicted components of the Egf receptor-ligand signaling system in teleost fishes (medaka, platyfish, stickleback, pufferfishes and zebrafish). We found two duplicated egfr genes, egfra and egfrb, in all available teleost genomes. Surprisingly only one copy for each of the seven Egfr ligands could be identified in most fishes, with zebrafish hbegf being the only exception. Special focus was put on medaka, for which we more closely investigated all Egf receptors and Egfr ligands. The different expression patterns of egfra, egfrb and their ligands in medaka tissues and embryo stages suggest differences in role and function. Preferential co-expression of different subsets of Egfr ligands corroborates the possible subfunctionalization and specialization of the two receptors in adult tissues. Bioinformatic analyses of the ligand-receptor interface between Egfr and its ligands show a very weak evolutionary conservation within this region. Using in vitro analyses of medaka Egfra, we could show that this receptor is only activated by medaka ligands, but not by human EGF. Altogether, our data suggest a lineage-specific Egfr/Egfr ligand co-evolution. Conclusions: Our data indicate that medaka Egfr signaling occurs via its two copies, Egfra and Egfrb, each of them being preferentially coexpressed with different subsets of Egfr ligands. This fish-specific occurrence of Egf receptor specialization offers unique opportunities to study the functions of different Egf receptor-ligand combinations and their biological outputs in vertebrates. Furthermore, our results strongly support the use of homologous ligands in future studies, as sufficient cross-specificity is very unlikely for this ligand/receptor system.
urn:nbn:de:bvb:20-opus-67922
6792
BMC EVOLUTIONARY BIOLOGY (2010) 10, DOI: 10.1186/1471-2148-10-27
Juliette A. G. C. Laisney
Ingo Braasch
Ronald B. Walter
Svenja Meierjohann
Manfred Schartl
deu
swd
Epidermaler Wachstumsfaktor-Rezeptor
eng
uncontrolled
epidermal growth factor receptor
eng
uncontrolled
Egfr
eng
uncontrolled
teleost fishes
Biowissenschaften; Biologie
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/5616/Schartl_1471_2148_10_27.pdf
14400
2015
eng
e0118214
2
10
article
1
2017-02-07
--
--
A transcriptome derived female-specific marker from the invasive Western mosquitofish (Gambusia affinis)
Sex-specific markers are a prerequisite for understanding reproductive biology, genetic factors involved in sex differences, mechanisms of sex determination, and ultimately the evolution of sex chromosomes. The Western mosquitofish, Gambusia affinis, may be considered a model species for sex-chromosome evolution, as it displays female heterogamety (ZW/ZZ), and is also ecologically interesting as a worldwide invasive species. Here, de novo RNA-sequencing on the gonads of sexually mature G. affinis was used to identify contigs that were highly transcribed in females but not in males (i.e., transcripts with ovary-specific expression). Subsequently, 129 primer pairs spanning 79 contigs were tested by PCR to identify sex-specific transcripts. Of those primer pairs, one female-specific DNA marker was identified, Sanger sequenced and subsequently validated in 115 fish. Sequence analyses revealed a high similarity between the identified sex-specific marker and the 3' UTR of the aminomethyl transferase (amt) gene of the closely related platyfish (Xiphophorus maculatus). This is the first time that RNA-seq has been used to successfully characterize a sex-specific marker in a fish species in the absence of a genome map. Additionally, the identified sex-specific marker represents one of only a handful of such markers in fishes.
PLoS ONE
10.1371/journal.pone.0118214
urn:nbn:de:bvb:20-opus-144004
PLoS ONE 10(2): e0118214 (2015). DOI: 10.1371/journal.pone.0118214
253511
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Dunja K. Lamatsch
Sofia Adolfsson
Alistair M. Senior
Guntram Christiansen
Maria Pichler
Yuichi Ozaki
Linnea Smeds
Manfred Schartl
Shinichi Nakagawa
eng
uncontrolled
sex chromosome evolution
eng
uncontrolled
linkage map
eng
uncontrolled
determination locus
eng
uncontrolled
poeciliid fishes
eng
uncontrolled
heterogamety
eng
uncontrolled
Cynoglossus semilaevis
eng
uncontrolled
determining genes
eng
uncontrolled
Y chromosome
eng
uncontrolled
sequence alignment
Physiologie und verwandte Themen
open_access
Theodor-Boveri-Institut für Biowissenschaften
OpenAIRE
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/14400/049_Lamatsch_PLoS_ONE.PDF
17084
2017
eng
7
6
article
1
2018-10-31
--
--
The draft genome of blunt snout bream (Megalobrama amblycephala) reveals the development of intermuscular bone and adaptation to herbivorous diet
The blunt snout bream Megalobrama amblycephala is the economically most important cyprinid fish species. As an herbivore, it can be grown by eco-friendly and resource-conserving aquaculture. However, the large number of intermuscular bones in the trunk musculature is adverse to fish meat processing and consumption. As a first towards optimizing this aquatic livestock, we present a 1.116-Gb draft genome of M. amblycephala, with 779.54 Mb anchored on 24 linkage groups. Integrating spatiotemporal transcriptome analyses, we show that intermuscular bone is formed in the more basal teleosts by intramembranous ossification and may be involved in muscle contractibility and coordinating cellular events. Comparative analysis revealed that olfactory receptor genes, especially of the beta type, underwent an extensive expansion in herbivorous cyprinids, whereas the gene for the umami receptor T1R1 was specifically lost in M. amblycephala. The composition of gut microflora, which contributes to the herbivorous adaptation of M. amblycephala, was found to be similar to that of other herbivores. As a valuable resource for the improvement of M. amblycephala livestock, the draft genome sequence offers new insights into the development of intermuscular bone and herbivorous adaptation.
GigaScience
10.1093/gigascience/gix039
urn:nbn:de:bvb:20-opus-170844
GigaScience 2017, Volume 6, Issue 7. DOI: 10.1093/gigascience/gix039
false
true
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Han Liu
Chunhai Chen
Zexia Gao
Jiumeng Min
Yongming Gu
Jianbo Jian
Xiewu Jiang
Huimin Cai
Ingo Ebersberger
Meng Xu
Xinhui Zhang
Jianwei Chen
Wei Luo
Boxiang Chen
Junhui Chen
Hong Liu
Jiang Li
Ruifang Lai
Mingzhou Bai
Jin Wei
Shaokui Yi
Huanling Wang
Xiaojuan Cao
Xiaoyun Zhou
Yuhua Zhao
Kaijian Wei
Ruibin Yang
Bingnan Liu
Shancen Zhao
Xiaodong Fang
Manfred Schartl
Xueqiao Qian
Weimin Wang
eng
uncontrolled
Megalobrama amblycephala
eng
uncontrolled
whole genome
eng
uncontrolled
herbivorous diet
eng
uncontrolled
intermuscular bone
eng
uncontrolled
transcriptome
eng
uncontrolled
gut microflora
Medizin und Gesundheit
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/17084/069_Liu_GIGASCIENCE.pdf
20147
2019
eng
213
10
article
1
2020-03-13
--
--
Analysis of the role of the Mc4r system in development, growth, and puberty of medaka
In mammals the melanocortin 4 receptor (Mc4r) signaling system has been mainly associated with the regulation of appetite and energy homeostasis. In fish of the genus Xiphophorus (platyfish and swordtails) puberty onset is genetically determined by a single locus, which encodes the mc4r. Wild populations of Xiphophorus are polymorphic for early and late-maturing individuals. Copy number variation of different mc4r alleles is responsible for the difference in puberty onset. To answer whether this is a special adaptation of the Mc4r signaling system in the lineage of Xiphophorus or a more widely conserved mechanism in teleosts, we studied the role of Mc4r in reproductive biology of medaka (Oryzias latipes), a close relative to Xiphophorus and a well-established model to study gonadal development. To understand the potential role of Mc4r in medaka, we characterized the major features of the Mc4r signaling system (mc4r, mrap2, pomc, agrp1). In medaka, all these genes are expressed before hatching. In adults, they are mainly expressed in the brain. The transcript of the receptor accessory protein mrap2 co-localizes with mc4r in the hypothalamus in adult brains indicating a conserved function of modulating Mc4r signaling. Comparing growth and puberty between wild-type and mc4r knockout medaka revealed that absence of Mc4r does not change puberty timing but significantly delays hatching. Embryonic development of knockout animals is retarded compared to wild-types. In conclusion, the Mc4r system in medaka is involved in regulation of growth rather than puberty.
Frontiers in Endocrinology
10.3389/fendo.2019.00213
urn:nbn:de:bvb:20-opus-201472
Frontiers in Endocrinology 2019, 10:213. doi: 10.3389/fendo.2019.00213
false
true
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Ruiqi Liu
Masato Kinoshita
Mateus C. Adolfi
Manfred Schartl
eng
uncontrolled
medaka
eng
uncontrolled
Mc4r
eng
uncontrolled
knockout
eng
uncontrolled
puberty
eng
uncontrolled
growth
Biowissenschaften; Biologie
Medizin und Gesundheit
open_access
Theodor-Boveri-Institut für Biowissenschaften
Förderzeitraum 2019
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/20147/Schartl_fendo-10-00213.pdf
5955
1994
eng
article
1
2012-06-28
--
--
Methodik und Anwendungsgebiete des genetischen Fingerabdruckverfahrens
No abstract available
urn:nbn:de:bvb:20-opus-61978
6197
In: Biologie in unserer Zeit (1994) 24, 9-14.
Deutsches Urheberrecht
T. Lubjuhn
Manfred Schartl
J. T. Epplen
deu
swd
Physiologische Chemie
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/5955/Schartl68.pdf
6847
1991
eng
article
1
2013-11-12
--
--
Rapid identification of recombinant baculoviruses using PCR
no abstract available
urn:nbn:de:bvb:20-opus-80328
8032
In: Biotechniques (1991) 11, S. 177-8.
Deutsches Urheberrecht
B. Malitschek
Manfred Schartl
deu
swd
PCR
Biowissenschaften; Biologie
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/6847/Schartl27.pdf
5287
1994
eng
article
1
2011-11-25
--
--
Autocrine stimulation of the Xmrk receptor tyrosine kinase in Xiphophorus melanoma cells and identification of a source for the physiological ligand
The melanoma·inducing gene of Xiphophorus fish encodes the Xmrk receptor tyrosine kinase. U sing a highly specific antiserum p~oduced against the recombinant receptor expressed with a baculovirus, it is shown that Xmrk is the most abundant phosphotyrosine protein in fish melanoma and thus highly activated in the tumors. Studies on a melanoma cellline revealed that these cells produce an activity that considerably stimulates receptor autophosphorylation. The stimulating activity induces receptor down-regulation and can be depleted from the melanoma cellsupernatant by the immobilized recombinant receptor protein. The fish melanoma cells can thus be considered autocrine tumor cells providing a source for future purification and characterization of the Xmrk ligand.
urn:nbn:de:bvb:20-opus-61551
6155
In: Journal of Biological Chemistry (1994) , 269, 14, 10423-10430
Deutsches Urheberrecht
Barbara Malitschek
Joachim Wittbrodt
Petra Fischer
Reiner Lammers
Axel Ullrich
Manfred Schartl
deu
swd
Physiologische Chemie
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/5287/Schartl02.pdf
19033
2019
eng
404
7
article
1
--
2019-10-24
--
Draft genome assembly and annotation of the Gila Topminnow Poeciliopsis occidentalis
No abstract available.
Frontiers in Ecology and Evolution
2296-701X
10.3389/fevo.2019.00404
urn:nbn:de:bvb:20-opus-190339
Frontiers in Ecology and Evolution, 2019, 7:404. doi: 10.3389/fevo.2019.00404
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Mariana Mateos
Du Kang
Christophe Klopp
Hugues Parrinello
Mateo García-Olazábal
Molly Schumer
Nathaniel K. Jue
Yann Guiguen
Manfred Schartl
eng
uncontrolled
genome assembly
eng
uncontrolled
genome annotation
eng
uncontrolled
transposable elements
eng
uncontrolled
topminnow
eng
uncontrolled
mitochondrial genome
Medizin und Gesundheit
open_access
Theodor-Boveri-Institut für Biowissenschaften
Import
Förderzeitraum 2019
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/19033/fevo-07-00404.pdf
20091
2019
eng
3688
9
article
1
2020-03-09
--
--
Allele-specific expression variation at different ploidy levels in Squalius alburnoides
Allopolyploid plants are long known to be subject to a homoeolog expression bias of varying degree. The same phenomenon was only much later suspected to occur also in animals based on studies of single selected genes in an allopolyploid vertebrate, the Iberian fish Squalius alburnoides. Consequently, this species became a good model for understanding the evolution of gene expression regulation in polyploid vertebrates. Here, we analyzed for the first time genome-wide allele-specific expression data from diploid and triploid hybrids of S. alburnoides and compared homoeolog expression profiles of adult livers and of juveniles. Co-expression of alleles from both parental genomic types was observed for the majority of genes, but with marked homoeolog expression bias, suggesting homoeolog specific reshaping of expression level patterns in hybrids. Complete silencing of one allele was also observed irrespective of ploidy level, but not transcriptome wide as previously speculated. Instead, it was found only in a restricted number of genes, particularly ones with functions related to mitochondria and ribosomes. This leads us to hypothesize that allelic silencing may be a way to overcome intergenomic gene expression interaction conflicts, and that homoeolog expression bias may be an important mechanism in the achievement of sustainable genomic interactions, mandatory to the success of allopolyploid systems, as in S. alburnoides.
Scientific Reports
10.1038/s41598-019-40210-8
urn:nbn:de:bvb:20-opus-200910
Scientific Reports (2019) 9:3688. https://doi.org/10.1038/s41598-019-40210-8
false
true
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Isa Matos
Miguel P. Machado
Manfred Schartl
Maria Manuela Coelho
eng
uncontrolled
Gene expression analysis
eng
uncontrolled
Transcription
eng
uncontrolled
Transcriptomic
Biowissenschaften; Biologie
open_access
Theodor-Boveri-Institut für Biowissenschaften
Förderzeitraum 2019
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/20091/Matos_ScientificReports_2019.pdf.pdf
14287
2011
eng
1-10
101
12
article
1
2017-01-11
--
--
Ploidy mosaicism and allele-specific gene expression differences in the allopolyploid \(Squalius\) \(alburnoides\)
Background
Squalius alburnoides is an Iberian cyprinid fish resulting from an interspecific hybridisation between Squalius pyrenaicus females (P genome) and males of an unknown Anaecypris hispanica- like species (A genome). S. alburnoides is an allopolyploid hybridogenetic complex, which makes it a likely candidate for ploidy mosaicism occurrence, and is also an interesting model to address questions about gene expression regulation and genomic interactions. Indeed, it was previously suggested that in S. alburnoides triploids (PAA composition) silencing of one of the three alleles (mainly of the P allele) occurs. However, not a whole haplome is inactivated but a more or less random inactivation of alleles varying between individuals and even between organs of the same fish was seen.
In this work we intended to correlate expression differences between individuals and/or between organs to the occurrence of mosaicism, evaluating if mosaics could explain previous observations and its impact on the assessment of gene expression patterns.
Results
To achieve our goal, we developed flow cytometry and cell sorting protocols for this system generating more homogenous cellular and transcriptional samples. With this set-up we detected 10% ploidy mosaicism within the S. alburnoides complex, and determined the allelic expression profiles of ubiquitously expressed genes (rpl8; gapdh and β-actin) in cells from liver and kidney of mosaic and non-mosaic individuals coming from different rivers over a wide geographic range.
Conclusions
Ploidy mosaicism occurs sporadically within the S. alburnoides complex, but in a frequency significantly higher than reported for other organisms. Moreover, we could exclude the influence of this phenomenon on the detection of variable allelic expression profiles of ubiquitously expressed genes (rpl8; gapdh and β-actin) in cells from liver and kidney of triploid individuals. Finally, we determined that the expression patterns previously detected only in a narrow geographic range is not a local restricted phenomenon but is pervasive in rivers where S. pyrenaicus is sympatric with S. alburnoides.
We discuss mechanisms that could lead to the formation of mosaic S. alburnoides and hypothesise about a relaxation of the mechanisms that impose a tight control over mitosis and ploidy control in mixoploids."
BMC Genetics
10.1186/1471-2156-12-101
urn:nbn:de:bvb:20-opus-142879
BMC Genetics 2011 12:101.
true
true
Isa Matos
Èlio Sucena
Miguel P Machado
Rui Gardner
Ângela Inácio
Manfred Schartl
Maria M Coelho
eng
uncontrolled
Squalius alburnoides
Medizin und Gesundheit
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/14287/157_Matos_BMC-Genetics.pdf
7113
1988
eng
article
1
2013-08-22
--
--
Different regulation of oncogene expression in tumor and embryonal cells of Xiphophorus
Melanoma formation in the poeciliid fish Xiphophorus is mediated primarily by a cellular oncogene, designated Tu. Elimination of Tu-specific genes releases the transforming function of Tu and leads to melanoma formation. Southern blot analyses revealed a tight linkage of a v-erb B related gene to the Tu-locus and Northern blot analyses of RNA of solid melanomas indicated a coordinated deregulation and for mutational activation of several oncogenes. In order to get a better insight into the regulation of oncogene expression in normal and transformed cells of Xiphophorus, we studied the expression of Xsrc, Xras, Xmyc, Xerb A, Xsis, and the v-erb B related gene in a melanoma derived cell line (PSM) and an embryonic cell line (A2) under conditions of low growth factor supply. Both celllines express the Xsrc, Xmyc, and Xras genes, while PSM cells in addition express the v-erb B related gene and A2 cells the Xsis gene. In PSM cells serum deprivation leads to an accumulation of most of the oncogene mRNAs analysed. This is most apparent for a 5.0 kb transcript of the v-erb B related gene, probably due to an increase in transcript stability. The levels of these mRNAs returned to normal within 2h after stimulation with 10% fetal calf serum. At the protein level we observed an initial decrease followed by an increase of the n-p60c-src kinase (the protein product of tbe Xsrc gene) activity in cells deprived of serum. Serum stimulation restored a normal pp60"-src kinase activity. In contrast serum deprivation of A2 cells reduced the transcript amounts of each of the oncogenes analysed. The same holds true for one beta-tubulin transcript, while the level of a second beta-tubulin transcript was unaffected. Serum stimulation led to a reactivation of Xras and Xsrc after a delay of approximately 48b. The pp60(c-src) kinase activity was found to be 6-10 times lower as compared to the PSM cells and did not differ between serum deprived and serum stimulated cells. Enzyme activities and isoenzyme patterns of several glycolytic enzymes were found to be not affected by serum deprivation and stimulation in both celllines.
8041
urn:nbn:de:bvb:20-opus-86240
In: Oncogene, 1988, 3, S. 113-122
Deutsches Urheberrecht
W. Maueler
A. Barnekow
E. Eigenbrodt
F. Raulf
H. F. Falk
A. Telling
Manfred Schartl
deu
swd
Schwertkärpfling
deu
swd
Tumorzelle
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/7113/Schartl_7113.pdf
5939
1987
eng
article
1
2012-06-25
--
--
Intermediary metabolism of normal and tumorous tissue of Xiphophorus (Teleostei: Poeciliidae)
No abstract available
urn:nbn:de:bvb:20-opus-61855
6185
In: Comparative biochemistry and physiology / B (1987) 88, 481-490.
Deutsches Urheberrecht
W. Maueler
E. Eigenbrodt
Manfred Schartl
deu
swd
Physiologische Chemie
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/5939/Schartl47.pdf
6229
2010
eng
article
1
2012-10-30
--
--
MMP13 mediates cell cycle progression in melanocytes and melanoma cells: in vitro studies of migration and proliferation
Background: Melanoma cells are usually characterized by a strong proliferative potential and efficient invasive migration. Among the multiple molecular changes that are recorded during progression of this disease, aberrant activation of receptor tyrosine kinases (RTK) is often observed. Activation of matrix metalloproteases goes along with RTK activation and usually enhances RTK-driven migration. The purpose of this study was to examine RTKdriven three-dimensional migration of melanocytes and the pro-tumorigenic role of matrix metalloproteases for melanocytes and melanoma cells. Results: Using experimental melanocyte dedifferentiation as a model for early melanomagenesis we show that an activated EGF receptor variant potentiates migration through three-dimensional fibrillar collagen. EGFR stimulation also resulted in a strong induction of matrix metalloproteases in a MAPK-dependent manner. However, neither MAPK nor MMP activity were required for migration, as the cells migrated in an entirely amoeboid mode. Instead, MMPs fulfilled a function in cell cycle regulation, as their inhibition resulted in strong growth inhibition of melanocytes. The same effect was observed in the human melanoma cell line A375 after stimulation with FCS. Using sh- and siRNA techniques, we could show that MMP13 is the protease responsible for this effect. Along with decreased proliferation, knockdown of MMP13 strongly enhanced pigmentation of melanocytes. Conclusions: Our data show for the first time that growth stimuli are mediated via MMP13 in melanocytes and melanoma, suggesting an autocrine MMP13-driven loop. Given that MMP13-specific inhibitors are already developed, these results support the evaluation of these inhibitors in the treatment of melanoma.
urn:nbn:de:bvb:20-opus-68335
6833
In: Molecular Cancer (2010) 9, DOI: 10.1186/1476-4598-9-201
Svenja Meierjohann
Anita Hufnagel
Elisabeth Wende
Markus A. Kleinschmidt
Katarina Wolf
Peter Friedl
Stefan Gaubatz
Manfred Schartl
deu
swd
Medizin
Biowissenschaften; Biologie
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/6229/Meierjohann058_1476_4598_9_201.pdf
6489
2012
eng
article
1
2013-03-19
--
--
Liver hyperplasia after tamoxifen induction of Myc in a transgenic medaka model
Myc is a global transcriptional regulator and one of the most frequently overexpressed oncoproteins in human tumors. It is well established that activation of Myc leads to enhanced cell proliferation but can also lead to increased apoptosis. The use of animal models expressing deregulated levels of Myc has helped to both elucidate its function in normal cells and give insight into how Myc initiates and maintains tumorigenesis. Analyses of the medaka (Oryzias latipes) genome uncovered the unexpected presence of two Myc gene copies in this teleost species. Comparison of these Myc versions to other vertebrate species revealed that one gene, myc17, differs by the loss of some conserved regulatory protein motifs present in all other known Myc genes. To investigate how such differences might affect the basic biological functions of Myc, we generated a tamoxifeninducible in vivo model utilizing a natural, fish-specific Myc gene. Using this model we show that, when activated, Myc17 leads to increased proliferation and to apoptosis in a dose-dependent manner, similar to human Myc. We have also shown that long-term Myc17 activation triggers liver hyperplasia in adult fish, allowing this newly established transgenic medaka model to be used to study the transition from hyperplasia to liver cancer and to identify Myc-induced tumorigenesis modifiers.
urn:nbn:de:bvb:20-opus-75316
7531
In: Disease Models & Mechanisms (2012) 5, 492-502, doi:10.1242/dmm.008730
Luciana Menescal
Cornelia Schmidt
Daniel Liedtke
Manfred Schartl
deu
swd
Biologie
Biowissenschaften; Biologie
open_access
Theodor-Boveri-Institut für Biowissenschaften
Förderzeitraum 2012
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/6489/23_dmm.008730.pdf
5288
1994
eng
article
1
2011-11-25
--
--
Recurrent origin of a sexually selected trait in Xiphophorus fishes inferred from a molecular phylogeny
DARWIN\(^1\) believed that sexual selection accounts for the evolution of exaggerated male ornaments, such as the sword-like caudal fin extensions of male fishes of the genus Xiphophorus, that appear detrimental to survival. Swordtails continue to feature prominently in empirical work and theories of sexual selection; the pre-existing bias hypothesis has been offered as an explanation for the evolution of swords in these fishes\(^{2,3}\). Based upon a largely morphological phylogeny, this hypothesis suggests that female preference to mate with sworded males arose in ancestrally swordless species, thus pre-dating the origin of the sword itself and directly driving its evolution. Here we present a molecular phylogeny (based on mitochondrial and nuclear DNA sequences) of Xiphophorus which differs from the traditional one: it indicates that the sword originated and was lost repeatedly. Our phylogeny suggests that the ancestor of the genus is more likely to have possessed a sword than not, thus questioning the applicability of the pre-existing bias hypothesis as an explanation for the cvolution of this sexually selected trait.
urn:nbn:de:bvb:20-opus-61569
6156
Nature (1994) , 368, 539-542
Deutsches Urheberrecht
Axel Meyer
Jean M. Morrissey
Manfred Schartl
deu
swd
Physiologische Chemie
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/5288/Schartl03.pdf
6973
1979
eng
article
1
2013-08-23
--
--
Eine neue Xiphophorus-Art aus Vera Cruz, Mexiko : (Pisces: Poeciliidae)
Xiphophorus andersi n. sp. from the Rio Atoyac, Vera Cruz, Mexico is described: lang head, moderately slender body, large dark black spar at the basis of the anal fin; adult male with short sword-like caudal appendage; rip of ray 5a of gonopodium without a developed claw. Xiphophorus andersi n. sp. differs by the combination of distinct characters from all the other species of the genus known so far. The new species shows features of both the so-called platyfish species group and the so-called swordtail species group.
8050
urn:nbn:de:bvb:20-opus-87124
In: Senckenbergiana / Senckenbergiana biologica, 1979, 60, 3/4, S. 147-151
Deutsches Urheberrecht
Manfred K. Meyer
Manfred Schartl
deu
swd
Schwertkärpfling
deu
swd
Veracruz <Stadt>
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/6973/Schartl_6973.pdf
7098
1984
eng
article
1
2013-08-23
--
--
Pseudotropheus (Maylandia) hajomaylandi n. sp., a new taxon from Lake Malawi
Pseudotropheus (Maylandia) hajomaylandi n. sp. un nouveau taxon du Lac Malawi
Pseudotropheus hajomaylandi (loc. typ. Isle of Chisumulu, Lake Malawi) is described as a new species. It is compared with Ps. aurora, Ps. greshakei, Ps. livingstonii, Ps. lombardoi, and Ps. zebra. All these taxa, including Ps. hajomaylandi and Ps. heteropictus, are classified in the subgenus Maylandia.
8046
urn:nbn:de:bvb:20-opus-70989
In: Revue française d'aquariologie, 1984, 11, S. 51-56
Deutsches Urheberrecht
Manfred K. Meyer
Manfred Schartl
deu
swd
Buntbarsche
deu
swd
Njassasee
Tiere (Zoologie)
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/7098/Schartl_7098.pdf
7112
1988
eng
article
1
2013-08-22
--
--
Expression of proto-oncogenes in embryonic, adult, and transformed tissue of Xiphophorus (Teleostei: Poeciliidae)
In Xiphophorus the causative, primary cellular oncogene for melanoma formation has been assigned by classical genetics to a sex-chromosomal locus, designated Tu. Activation of Tu was proposed to be the result of the elimination of Tu-specific regulatory genes which normally suppress the transforming function in the nontumorous state. In order to understand the role which known proto-oncogenes migbt play in this process, we have analysed the expression of src, erb A, erb B, ras, abl, sis and mil related genes from Xiphophorus during embryogenesis, in non-tumorous organs and in melanoma cells. For src, ras, erb B and sis a differential expression during embryogenesis and/or in normal organs was detected, with preferential expression of src in neural tissues, a high abundance of sis transcripts in an embryonal epitheloid cellline and of erbB transcripts in the head nephros. In melanoma cells ras, src and a v-erb B related gene were found to be expressed. The src gene most likely is more involved in secondary processes during tumor progression, while the expression of the v-erb B related gene might be transformation-specific because recently such a sequence was found to map to the close vicinity of the Tu-locus.
8040
urn:nbn:de:bvb:20-opus-86233
In: Oncogene, 1988, 2, S. 421-430
Deutsches Urheberrecht
Winfried Mäueler
Friedrich Raulf
Manfred Schartl
deu
swd
Schwertkärpfling
deu
swd
Protoonkogen
deu
swd
Gewebe
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/7112/Schartl_7112.pdf
5316
1993
eng
article
1
2011-12-01
--
--
Different expression patterns of oncogenes and proto-oncogenes in hereditary and carcinogen-induced tumors of Xiphophorus
Hereditary melanoma in Xiphophorus hybrids canying the melanoma·induclng Tu-Sd locus is caused by transcriptional activation of the Xmrk gene that resides at the Tu·Sd locus and encodes a novel member of receptor tyrosine kinases (RTK). ln this study, a total of 17 hereditary melanomas from various hybrid genotypes harbouring 7 different Tu alleles were also found to aver-express the correspondlng Xmrlc alleles. The Ievei of over-expression correlated with the degree of malignancy of the melanoma. ln addition, Xsrc expression was high ln many malignant melanomas. Expression pattems and Ieveis of the Xiphophorus EGF-receptor gene (Xerb B), the c-myc (Xmyc), and the PDGF (Xsls) gene(s) were not intriguing. Transcription of the ras gene(s) may be correlated to secondary events of melanoma progression. Expression pattems of Xfms, the Xiphophorus CSF-1 receptor homologue, can be explained by different contents of infiltrating macrophages in the tumors. ln carcinogen-induced tumors includlng one melanoma no significant expression of the Xmrk oncogene could be detected. Xsrc expression, however, was strikingly high. This indicates that activation of oncogenes other than Xmrk ls instrumental in tumorigenesls of neoplasia of non-hereditary origin.
urn:nbn:de:bvb:20-opus-61592
6159
In: International Journal of Cancer (1993) , 55, 288-296
Deutsches Urheberrecht
Winfried Mäueler
Angelika Schartl
Manfred Schartl
deu
swd
Physiologische Chemie
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/5316/Schartl07.pdf
5315
1994
eng
article
1
2011-12-01
--
--
Sex chromosome polymorphism and heterogametic males revealed by two cloned DNA probes in the ZW/ZZ fish Leporinus elongatus
In order to study the divergence of teleost sex chromosomes, subtractive cloning was carried out between genomic DNA ofmales and females ofthe rainbow trout (XX/XY) and of Leporinus elongatus (ZW /ZZ). Inserts cloned in a plasmid vector were individually tested on Southern blots of DNA of males and females for sex specificity. No sex-specific insert was obtained from trout, but two out of ten inserts cloned from L. elongatus showed sex-specific patterns in this species: one corresponds to a sequence present on both Z and W chromosomes, while the other is W specific. Sequences of these two inserts show neither clear homology with other known sequences, nor an open reading frame. They cross-hybridize with the genomic DNA of Leporinusfriderici, but without sex-specific patterns. Twenty-four L. elongatus adults were sexed by gonadal observation, chromosomed examination and Southern hybridization with one or the other insert. Ten males and 11 females had chromosomes and hybridization patterns typical of their sex. One ZW female was recognized as a male with the W-specific probe. This was also the case for two unusual ZW males, one having a male hybridization pattern with the other probe. These three atypical individuals may result from single genetic exchanges between four regions of the Z and the W, giving rise to three atypical W chromosomes. Finding males with such atypical heterochromosomes in a female heterogametic species may indicate that a gradual transition occurs between the heterogametic systems.
urn:nbn:de:bvb:20-opus-61583
6158
In: Chromosoma (1994) , 103, 31-9
Deutsches Urheberrecht
Ichiro Nakayama
Fausto Foresti
Rita Tewari
Manfred Schartl
Daniel Chourrout
deu
swd
Physiologische Chemie
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/5315/Schartl06.pdf
5339
1993
eng
article
1
2011-12-07
--
--
Primitive sex chromosomes in poeciliid fishes harbor simple repetitive DNA sequences
The demonstration ofthe chromosomal mode ofsex determinationvia genetic experiments as well as the absence of heteromorphic sex chromosomes affirm poeciliid fishes as a unique group among vertebrates that are endowed with the mostprimitive form of sex chromosornes. In many different taxa the evolutionary process involved in the differentiation ofadvanced sex chromosomes is outlined through sex specifically organized repetitive sequences. In this investigation hydridization of synthetic probes specific to genomic simple repeat motifs uncovers a sex-specific hybridization pattern in certain viviparaus fishes ofthe family Poeciliidae. The hybridization pattern together with specific staining ofthe constitutive heterochromatin by C-banding reveals heterogamety in males (Poecilia reticulata) as weil as in females (P. sphenops). In P. velifera, however, C-banding alone fails to unravel the heterogametic status. The female specific W-chromosome can be detected by simple repetitive sequence probes. Therefore, the principal significance of heterochromatization as a means of generating differentiated sex chromosomes is evident.
urn:nbn:de:bvb:20-opus-61659
6165
In: Journal of Experimental Zoology (1993) , 265, 301-308
Deutsches Urheberrecht
Indrajit Nanda
Manfred Schartl
Jörg T. Epplen
Wolfgang Feichtinger
Michael Schmid
deu
swd
Physiologische Chemie
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/5339/Schartl13.pdf
5366
1992
eng
article
1
2011-12-09
--
--
Early stages of sex chromosome differentiation in fish as analysed by simple repetitive DNA sequences
Animal sex chromosome evolution has started on different occasions with a homologous pair of autosomes leading to morphologically differentiated gonosomes. In contrast to other vertebrate classes, among fishes cytologically dernonstrahle sex chromosomes are rare. In reptiles, certain motifs of simple tandemly repeated DNA sequences like (gata)\(_n\)/(gaca)\(_m\) are associated with the constitutive heterochromatin of sex chromosomes. In this study a panel of simple repetitive sequence probes was hybridized to restriction enzyme digested genomic DNA of poeciliid fishes. Apparent male heterogamety previously established by genetic experiments in Poecilia reticulata (guppy) was correlated with male-specific hybridization using the (GACA)\(_4\) probe. The (GATA)\(_4\) oligonucleotide identifies certain male guppies by a Y chromosomal polymorphism in the outbred population. In cantrast none of the genetically defined heterogametic situations in Xiphophorus could be verified consistently using the collection of simple repetitive sequence probes. Only individuals from particular populations produced sex-specific patterns of hybridization with (GATA)\(_4\). Additional poeciliid species (P. sphenops, P. velifera) harbour different sex-specifically organized simple repeat motifs. The observed sex-specific hybridization patterns were substantiated by banding analyses of the karyotypes and by in situ hybridization using the (GACA)\(_4\) probe.
urn:nbn:de:bvb:20-opus-61715
6171
Chromosoma (1992) , 101, 301-310
Deutsches Urheberrecht
Indrajit Nanda
Manfred Schartl
Wolfgang Feichtinger
Jörg T. Epplen
Michael Schmid
deu
swd
Physiologische Chemie
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/5366/Schartl22.pdf
26724
2022
eng
7
11
article
1
--
2022-03-25
--
Evolution of the degenerated Y-chromosome of the swamp guppy, Micropoecilia picta
The conspicuous colour sexual dimorphism of guppies has made them paradigmatic study objects for sex-linked traits and sex chromosome evolution. Both the X- and Y-chromosomes of the common guppy (Poecilia reticulata) are genetically active and homomorphic, with a large homologous part and a small sex specific region. This feature is considered to emulate the initial stage of sex chromosome evolution. A similar situation has been documented in the related Endler’s and Oropuche guppies (P. wingei, P. obscura) indicating a common origin of the Y in this group. A recent molecular study in the swamp guppy (Micropoecilia. picta) reported a low SNP density on the Y, indicating Y-chromosome deterioration. We performed a series of cytological studies on M. picta to show that the Y-chromosome is quite small compared to the X and has accumulated a high content of heterochromatin. Furthermore, the Y-chromosome stands out in displaying CpG clusters around the centromeric region. These cytological findings evidently illustrate that the Y-chromosome in M. picta is indeed highly degenerated. Immunostaining for SYCP3 and MLH1 in pachytene meiocytes revealed that a substantial part of the Y remains associated with the X. A specific MLH1 hotspot site was persistently marked at the distal end of the associated XY structure. These results unveil a landmark of a recombining pseudoautosomal region on the otherwise strongly degenerated Y chromosome of M. picta. Hormone treatments of females revealed that, unexpectedly, no sexually antagonistic color gene is Y-linked in M. picta. All these differences to the Poecilia group of guppies indicate that the trajectories associated with the evolution of sex chromosomes are not in parallel.
Cells
2073-4409
10.3390/cells11071118
urn:nbn:de:bvb:20-opus-267242
2022-04-13T07:28:21+00:00
sword
swordwue
attachment; filename=deposit.zip
2a20f9dc7b49d2b61e7c5fe7d433f8d1
Cells (2022) 11:7, 1118. https://doi.org/10.3390/cells11071118
false
true
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Indrajit Nanda
Susanne Schories
Ivan Simeonov
Mateus Contar Adolfi
Kang Du
Claus Steinlein
Manfred Alsheimer
Thomas Haaf
Manfred Schartl
eng
uncontrolled
sex chromosomes
eng
uncontrolled
heterochromatin
eng
uncontrolled
Y chromosome degeneration
eng
uncontrolled
meiosis
eng
uncontrolled
synaptonemal complex
eng
uncontrolled
recombination
eng
uncontrolled
5-methylcytosine
eng
uncontrolled
testosterone
eng
uncontrolled
sexual antagonistic genes
eng
uncontrolled
sex linked pigmentation pattern
Biowissenschaften; Biologie
open_access
Institut für Humangenetik
Theodor-Boveri-Institut für Biowissenschaften
Import
Förderzeitraum 2022
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/26724/cells-11-01118-v2.pdf
6845
1992
eng
article
1
2013-11-11
--
--
Multiple src-related kinase genes, srk1-4, in the fresh water sponge Spongilla lacustris
In one of the simplest metazoan organisms, the sponge Spongilla lacustris, at least four different src-related kin ase genes (srkl-4) are expressed, aD of which show a high degree of similarity to the c-src genes of vertebrates. Whereas srk2 and srk3 are c1early unrelated at the nucleic acid level, srkl and srk4 share identical sequences in the 5' parts of their cDNAs. The cloning of several primer extension clones and genomic polymerase chain re action experiments confirmed the hypo thesis of an alternative splicing of tandemly arranged carboxyterminal parts of srkl and srk4. The genomic sequence encoding both proteins was found to be interrupted at the splice point by an intron which is located in the same position as one of the introns in the chicken src gene, which is the only gene conserved in invertebrates and vertebrates. All four srk genes are expressed in adult sponges as mRNA transcripts of about 2.2 kb. Tyrosine kin ase activity of a src-related kin ase could be detected in adult sponges but not in their resting form (gemmulae), and may reflect the activity of the srk protein products. Spongilla lacustris is the simplest organism from which a pro tein tyrosine kinase gene has been isolated. The presence of at least four such genes in the evolutionary ancient and primitive phylum Porifera suggests that tyrosine kinase genes arose concomitantly with or shortly after the appearance of multicellular organisms and that their activity may be involved in aggregation and cell-cell recognition.
urn:nbn:de:bvb:20-opus-80282
8028
In: Oncogene (1992) 7, 1625-30
Deutsches Urheberrecht
S. Ottilie
F. Raulf
A. Barnekow
G. Hannig
Manfred Schartl
deu
swd
Spongilla lacustris
Medizin und Gesundheit
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/6845/Schartl17.pdf
6937
1985
eng
conferenceobject
1
2013-08-22
--
--
Pigment pattern formation during embryogenesis in Xiphophorus
No abstract available.
8043
urn:nbn:de:bvb:20-opus-69370
In: Biological, Molecular and Clinical Aspects of Pigmentation : proceedings of the XIIth Internat. Pigment Cell Conference, Gießen, Federal Republic of Germany Pigment cell nineteen hundred and eighty-five / ed. by Joseph T. Bagnara. - [Tokyo]: Univ. of Tokyo Pr., 1985. - S. 291-302. - ISBN 4-13-068114-1
Deutsches Urheberrecht
R. U. Peter
Manfred Schartl
F. Anders
H.-R. Duncker
deu
swd
Schwertkärpfling
deu
swd
Embryonalentwicklung
deu
swd
Pigmentmuster
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/6937/Schartl_6937.pdf
5928
1989
eng
article
1
2012-06-19
--
--
Evolution of the neuron-specific alternative splicing product of the c-src proto-oncogene
The observation of a slower migrating form of pp6oc-src in neural tissue of chicken and mouse has recently been shown to be due to an alternative transcript form of tbe c-src gene (Martinez et al.: Science 237:411-415, 1987; Levy et al.: Mol Cell Bio17:4142- 4145, 1987). An insertion of 18 basepairs between exons 3 and 4, presumed to be due to alternative splicing of a mini-exon, gives rise to six amino acid residues not found in the non-neuronal (termed flbroblastic) form of pp60\(^{c-src}\). Wehave addressed the question of the evolutionary origin of the c-src neuronal insert · and its functional signiflcance regarding neural-speciflc expression of the c-src gene. To this end we have investigated whether the c-src gene of a lower verlebrate (the teleost fish Xiphophorus) gives rise to a neural-specific transcript in an analogous manner. We could show that the fish c-src gene does encode for a "fibroblastic" and a "neuronal" form of transcript and that the neuronal transcript does indeed arise by way of alternative splicing of a mini-exon. The miniexon is also 18 basepairs long and we could demoostrate directly that this exon lies within the intron separating exons 3 and 4. For comparative purposes we have examined whether the fish c-yes gene, the member of the src gene family most closely related to c-src, also encodes a neural tissue-specific transcript. No evidence for a second transcript form in brain was obtained. This result suggests that the mini-exon arose within the c-src gene lineage sometime between the srclyes gene duplication event and the divergence of the evolutionary lineage giving rise to the teleost fish. Published genomic sequence of src-related genes in Drosophila and our own results with Hydra demoostrate no intron in these species at the analogous location, consistent with first appearance of this mini-exon sometime between 550 and 400 million years ago.
urn:nbn:de:bvb:20-opus-61796
6179
Journal of Neuroscience Research (1989) 24, 81-8.
Deutsches Urheberrecht
F. Raulf
S. M. Robertson
Manfred Schartl
deu
swd
Physiologische Chemie
eng
uncontrolled
Xiphophorus
eng
uncontrolled
teleost flsh
eng
uncontrolled
polymerase
eng
uncontrolled
chain reaction
eng
uncontrolled
RT -PCR
eng
uncontrolled
mini-exon
eng
uncontrolled
pp6oc-src
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/5928/Schartl37.pdf
7111
1989
eng
article
1
2013-08-22
--
--
Localization of cellular src mRNA during development and in the differentiated bipolar neurons of the adult neural retina in Xiphophorus
The expression of the c-src gene in embryonie and adult tissue of the teleost fish Xiphophorus helleri was analyzed by in-situ hybridization. The highly conserved fish c-src gene was found to be expressed at high levels in midterm embryos, where c-src mRNA was localized in developing neurons of the sensory layer of the differentiating retina and in the developing brain. In adult tissues the expression of c-src was found to persist in certain cell types of the brain and the neural retina, especially in the bipolar cells of the inner nuclear layer, which are postmitotic, fully differentiated mature neurons. Thus c-src in Xiphophorus appears to be a developmentally regulated proto-oncogene which is important for neuronal differentiation during organogenesis, but whose persistence of expression in certain terminally differentiated neurons strongly suggests a particular maintenance function for c-src in these cells as well.
8039
urn:nbn:de:bvb:20-opus-86703
In: Oncogene research, 1989, 5, S. 39-47
Deutsches Urheberrecht
Friedrich Raulf
Winfried Mäueler
Scott M. Robertson
Manfred Schartl
deu
swd
Schwertkärpfling
deu
swd
Messenger-RNS
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/7111/Schartl_7111.pdf
5945
1984
eng
article
1
2012-06-26
--
--
A Transmission Electron Microscopical and Freeze-Etch Study of Malignant-Melanoma in Fish
No abstract available
urn:nbn:de:bvb:20-opus-61916
6191
In: Yale Journal of Biology and Medicine (1984) 57, 413-413.
Deutsches Urheberrecht
R. Riehl
Manfred Schartl
deu
swd
Physiologische Chemie
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/5945/Schartl57.pdf
5946
1984
eng
article
1
2012-06-26
--
--
Comparative studies on the ultrastructure of malignant melanoma in fish and human by freeze-etching and transmission electron microscopy
Malignant melanomas (MM) in the fish Xiphophorus and in humans were studied both by transmission electron microscopy (TEM) and freeze-etching (FE). In both fish and human melanomas the cells show interdigitations of the,plasma membranes. The nuclei are large and lobulated and have many nuclear pores. Melanosomes are abundant and melanosome complexes ("compound melanosomes") occur regularly. Pinocytotic vesicles could be demonstrated in fish and human melanomas showing iocal differences in frequency and distribution patterns in the tumor. lntercellular junctions are lacking in MM cells from fish and humans. The FE technique showed considerable advantages in demonstrating membrane-surface peculiarities such as nuclear pores or pinocytotic vesicles. The FE replicas of fish melanomas are like those of humans. These findings may support the hypothesis that melanoma in fish and humans reflect the same biological phenomenon.
urn:nbn:de:bvb:20-opus-61920
6192
In: Journal of cancer research and clinical oncology (1984) 107, 21-31.
Deutsches Urheberrecht
R. Riehl
Manfred Schartl
G. Kollinger
deu
swd
Physiologische Chemie
eng
uncontrolled
Malignant melanoma
eng
uncontrolled
Fish
eng
uncontrolled
Human
eng
uncontrolled
Freeze-etching
eng
uncontrolled
Transmission electron microscopy
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/5946/Schartl58.pdf
7097
1985
eng
conferenceobject
1
2013-08-23
--
--
An ultrastructural study of melanoma in Xiphophorus
Melanotic melanoma (MM) of Xiphophorus (Teleostei: Poeciliidae) was studied by conventional preparations and freeze-etch preparations for electron microscopy. MM of Xiphophorus exhibits tightly packed pigment cells with prominent dendritic processes and interdigitations of their plasma membranes. The most impressive feature of MM cells is the occurrence of Iarge lobulated nuclei with numerous nuclear pores and some nuclear pockets. Abundant spheroidal or ellipsoidal melanosomes (diameter 200-650 nm) and vesicular structures are distributed throughout the cellular dendrites, whereas the perinucJear cytoplasm is free of melanosomes.
A further characteristic feature of melanoma cells in fish is the occurrence of melanosome complexes (i.e., "compound melanosomes"). These melanosome complexes consist of a few to numerous melanosomes, which are enveloped by a separate rnembrane. Pinocytotic vesicles couJd be demonstrated with distinct differences in frequency and distribution patterns, indicating differences in the metabolic activities of the cells in the same melanoma. Intercellular junctions are lacking in the MM cells.
The conventional TEM technique showed clear advantages in the demonstration of intemal architecture of organelles, whereas FE bad considerable potential in respect to the visualization of membrane surface specializations.
8045
urn:nbn:de:bvb:20-opus-70978
In: Biological, Molecular and Clinical Aspects of Pigmentation : proceedings of the XIIth Internat. Pigment Cell Conference, Gießen, Federal Republic of Germany Pigment cell nineteen hundred and eighty-five / ed. by Joseph T. Bagnara. - [Tokyo]: Univ. of Tokyo Pr., 1985. - S. 457-464. - ISBN 4-13-068114-1
Deutsches Urheberrecht
Rüdiger Riehl
Manfred Schartl
Fritz Anders
deu
swd
Schwertkärpfling
deu
swd
Krebs <Medizin>
deu
swd
Ultrastruktur
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/7097/Schartl_7097.pdf
5953
1994
eng
article
1
2012-06-28
--
--
Evolutionary origin and molecular biology of the melanoma-inducing oncogene of Xiphophorus
No abstract available
urn:nbn:de:bvb:20-opus-61954
6195
In: Pigment Cell Res (1994) 7, 428-32.
Deutsches Urheberrecht
A. Schartl
N. Dimitrijevic
Manfred Schartl
deu
swd
Physiologische Chemie
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/5953/Schartl65.pdf
6990
1982
eng
article
1
2013-08-23
--
--
Promotion and regression of neoplasia by testosterone-promoted cell differentiation in Xiphophorus and Girardinus
No abstract available.
8048
urn:nbn:de:bvb:20-opus-86684
In: Carcinogenesis, 1982, 7, S. 427-434
Deutsches Urheberrecht
A. Schartl
Manfred Schartl
F. Anders
deu
swd
Schwertkärpfling
deu
swd
Lebendgebärende Zahnkarpfen
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/6990/Schartl_6990.pdf
6972
1981
eng
conferenceobject
1
2013-08-23
--
--
Phenotypic conversion of malignant melanoma to benign melanoma and vice versa in Xiphophorus
No abstract available.
8049
urn:nbn:de:bvb:20-opus-86662
In: Phenotypic expression in pigment cells : pigment cell 1981 ; proceedings of the XIth International Pigment Cell Conference, Sendai, Japan, 1980 / ed. by Makoto Seiji. - [Tokyo] : Univ. of Tokyo Press, [1981]. - S. 507-514. - ISBN 0-86008-293-8
Deutsches Urheberrecht
A. Schartl
Manfred Schartl
F. Anders
deu
swd
Schwertkärpfling
deu
swd
Krebs <Medizin>
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/6972/Schartl_6972.pdf
7267
1990
eng
article
1
2013-09-03
--
--
Genes and cancer: Molecular biology of the melanoma oncogene of Xiphophorus
No abstract available.
8068
urn:nbn:de:bvb:20-opus-72670
In: Biotech Forum Europe, 1990, 7, S. 461-466
Deutsches Urheberrecht
Angelika Schartl
Manfred Schartl
deu
swd
Schwertkärpfling
deu
swd
Krebs <Medizin>
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/7267/Schartl_7267.pdf
5938
1988
eng
article
1
2012-06-25
--
--
A sex chromosomal restriction-fragment-length marker linked to melanoma-determining Tu loci in Xiphophorus
No abstract available
urn:nbn:de:bvb:20-opus-61842
6184
In: Genetics (1988 ) 119, 679-85.
Deutsches Urheberrecht
Manfred Schartl
deu
swd
Physiologische Chemie
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/5938/Schartl46.pdf
5372
1990
eng
article
1
2011-12-12
--
--
Homology of melanoma-inducing loci in the genus Xiphophorus
Several species of the genus Xiphophorus are polymorphic for specific pigment patterns. Same of these give rise to malignant melanoma following the appropriate crossings. For one of these pattern Iod from the platyfish Xiphophorus maculatus the melanoma-inducing gene has been doned and found to encode a novel receptor tyrosine kinase, designated Xmrk. Using molecular probes from this gene in Southern blot analyses on single fish DNA preparations from 600 specimens of different populations of various species of the genus Xiphophorus and their hybrids, either with or without melanomapredisposing pattern, it was shown that all individuals contain the Xmrk gene as a proto-oncogene. It is located on the sex chromosome. All fish that carry a melanoma-predisposing locus which has been identified by Mendelian genetics contain an additional copy of Xmrk, closely linked to a specific melanophore pattern locus on the sex chromosome. The melanoma-inducing loci of the different species and populations are homologous. The additional copy of Xmrk obviously arose by a geneduplication event, thereby acquiring the oncogenic potential. The homology of the melanomainducing Iod points to a similar mechanism of tumor suppression in all feral fish populations of the different species of the genus Xiphophorus.
urn:nbn:de:bvb:20-opus-61757
6175
In: Genetics (1990 ) 126, 1083-1091
Deutsches Urheberrecht
Manfred Schartl
deu
swd
Physiologische Chemie
eng
uncontrolled
Xiphophorus
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/5372/Schartl32.pdf
11991
2014
eng
2
7
article
1
2015-10-07
--
--
Beyond the zebrafish: diverse fish species for modeling human disease
In recent years, zebrafish, and to a lesser extent medaka, have become widely used small animal models for human diseases. These organisms have convincingly demonstrated the usefulness of fish for improving our understanding of the molecular and cellular mechanisms leading to pathological conditions, and for the development of new diagnostic and therapeutic tools. Despite the usefulness of zebrafish and medaka in the investigation of a wide spectrum of traits, there is evidence to suggest that other fish species could be better suited for more targeted questions. With the emergence of new, improved sequencing technologies that enable genomic resources to be generated with increasing efficiency and speed, the potential of non-mainstream fish species as disease models can now be explored. A key feature of these fish species is that the pathological condition that they model is often related to specific evolutionary adaptations. By exploring these adaptations, new disease-causing and disease-modifier genes might be identified; thus, diverse fish species could be exploited to better understand the complexity of disease processes. In addition, non-mainstream fish models could allow us to study the impact of environmental factors, as well as genetic variation, on complex disease phenotypes. This Review will discuss the opportunities that such fish models offer for current and future biomedical research.
Disease Models & Mechanisms
10.1242/dmm.012245
1754-8411
urn:nbn:de:bvb:20-opus-119919
Disease Models & Mechanisms (2014) 7, 181-192 doi:10.1242/dmm.012245
Manfred Schartl
eng
uncontrolled
evolutionary mutant model
eng
uncontrolled
natural variation
eng
uncontrolled
cancer
eng
uncontrolled
fish model
Physiologie und verwandte Themen
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/11991/066_Schartl_Disease_Models_&_Mechanisms.pdf
5978
1992
eng
article
1
2012-07-02
--
--
Molecular cloning, structural characterization, and analysis of transcription of the melanoma oncogene of xiphophorus
No abstract available
urn:nbn:de:bvb:20-opus-61989
6198
In: Pigment Cell Research (1992 ) Suppl 2, 173-80.
Deutsches Urheberrecht
Manfred Schartl
Dieter Adam
deu
swd
Physiologische Chemie
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/5978/Schartl73.pdf
5943
1984
eng
article
1
2012-06-26
--
--
Differential expression of the cellular src gene during vertebrate development
No abstract available
urn:nbn:de:bvb:20-opus-61893
6189
In: Developmental biology (1984) 105, 415-22.
Deutsches Urheberrecht
Manfred Schartl
A. Barnekow
deu
swd
Physiologische Chemie
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/5943/Schartl55.pdf
5944
1984
eng
article
1
2012-06-26
--
--
Cellular src gene product detected in the freshwater sponge Spongilla lacustris
No abstract available
urn:nbn:de:bvb:20-opus-61904
6190
In: Molecular and Cellular Biology (1984) 4, 1179-81.
Deutsches Urheberrecht
Manfred Schartl
A. Barnekow
deu
swd
Physiologische Chemie
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/5944/Schartl56.pdf
5951
1982
eng
article
1
2012-06-28
--
--
Correlations of inheritance and expression between a tumor gene and the cellular homolog of the Rous sarcoma virus-transforming gene in Xiphophorus
No abstract available
urn:nbn:de:bvb:20-opus-61937
6193
In: Cancer Research (1982) 42, 4222-7.
Deutsches Urheberrecht
Manfred Schartl
A. Barnekow
H. Bauer
F. Anders
deu
swd
Physiologische Chemie
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/5951/Schartl61.pdf
7099
1982
eng
article
1
2013-08-23
--
--
The expression in eukaryotes of a tyrosine kinase which is reactive with pp60v-src antibodies
All specimens of Eumetazoa and Parazoa, ranging from mammals, birds, teleosts, sharks, lampreys, amphioxus, insects, down to sponges showed the pp60c-src associated kinase activity, indicating that c-src, which is the cellular homologue of the oncogene v-src of Rous sarcoma virus (RSV) is probably present in all multicellular animals. Protozoa and plants did not show pp60c-src: kinase activity.
The degree of c-src expression depends on the taxonomic rank of the Eumetazoa tested, and is organ-specific with nervaus tissues displaying the highest kinase activities. In the central nervous system of mammals and birds we found a high c-src expression, and in that of the lampreys, amphioxus, and insects the lowest. Unexpectedly, total extracts of sponges showed an amount of pp60c-src kinase activity similar to that of brain cell extracts of mammals and birds. These findings suggest that pp60c-src is a phylogenetic old protein that might have evolved together with the multicellular organisation of Metazoa, and that might be of importance in proliferation and differentiation of nontransformed cells.
8047
urn:nbn:de:bvb:20-opus-86208
In: Differentiation, 1982, 23, S. 109-14
Deutsches Urheberrecht
Manfred Schartl
Angelika Barnekow
deu
swd
Protein-Tyrosin-Kinasen
deu
swd
Eukaryoten
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/7099/Schartl_7099.pdf
6977
1993
eng
bookpart
1
2013-08-23
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High mating success of low rank males in Limia perugiae (Pisces: Poeciliidae) as determined by DNA-fingerprinting
Hierarchical structures among male individuals in a population are frequently reflected in differences in aggressive and reproductive behaviour and access to the females. In general social dominance requires large investments which in turn may have to be compensated for by high reproductive success. However, this hypothesis has so far only been sufficiently tested in small mating groups due to the difficulties of determining paternity by classical methods using non-molecular markers. DNA fingerprinting overcomes these problems offering the possibility to determine genetic relationships and mating patterns within larger groups. Using this approach we have recently shown (Schartl et al., 1993) that in the poeciliid fish Limia perugiae in small mating groups the dominant male has 100% mating success, while in larger groups its contribution to the offspring unexpectedly drops to zero. The reproductive failure under such social conditions is explained by the inability of the ex-male to protect all the females simultaneously against mating attempts of his numerous subordinate competitors.
8054
urn:nbn:de:bvb:20-opus-87132
In: DNA Fingerprinting : state of sciences / Sergio D. Pena (Hrsg.). - Basel [u.a.]: Birkhäuser, 1993. - S. 363-370. - ISBN 3-7643-2906-8
Deutsches Urheberrecht
Manfred Schartl
C. Erbelding-Denk
S. Hölter
I. Nanda
M. Schmid
J. H. Schröder
J. T. Epplen
deu
swd
DNS
deu
swd
Fingerprint-Verfahren
deu
swd
Lebendgebärende Zahnkarpfen
Chemie und zugeordnete Wissenschaften
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/6977/Schartl_6977.pdf