12846
2013
eng
e71644
8
8
article
1
2016-03-02
--
--
SDS Interferes with SaeS Signaling of Staphylococcus aureus Independently of SaePQ
The Staphylococcus aureus regulatory saePQRS system controls the expression of numerous virulence factors, including extracellular adherence protein (Eap), which amongst others facilitates invasion of host cells. The saePQRS operon codes for 4 proteins: the histidine kinase SaeS, the response regulator SaeR, the lipoprotein SaeP and the transmembrane protein SaeQ. S. aureus strain Newman has a single amino acid substitution in the transmembrane domain of SaeS (L18P) which results in constitutive kinase activity. SDS was shown to be one of the signals interfering with SaeS activity leading to inhibition of the sae target gene eap in strains with SaeS(L) but causing activation in strains containing SaeS(P). Here, we analyzed the possible involvement of the SaeP protein and saePQ region in SDS-mediated sae/eap expression. We found that SaePQ is not needed for SDS-mediated SaeS signaling. Furthermore, we could show that SaeS activity is closely linked to the expression of Eap and the capacity to invade host cells in a number of clinical isolates. This suggests that SaeS activity might be directly modulated by structurally non-complex environmental signals, as SDS, which possibly altering its kinase/phosphatase activity.
PLOS ONE
10.1371/journal.pone.0071644
1932-6203
urn:nbn:de:bvb:20-opus-128469
PLoS ONE 8(8): e71644. doi:10.1371/journal.pone.0071644
Phuti E. Makgotlho
Gabriella Marincola
Daniel Schäfer
Quian Liu
Taeok Bae
Tobias Geiger
Elizabeth Wasserman
Christine Wolz
Wilma Ziebuhr
Bhanu Sinha
eng
uncontrolled
host-cell invasion
eng
uncontrolled
2-component system
eng
uncontrolled
strain Newman
eng
uncontrolled
allelic replacement
eng
uncontrolled
genome sequence
eng
uncontrolled
locus
eng
uncontrolled
gene
eng
uncontrolled
activation
eng
uncontrolled
expression
eng
uncontrolled
infection
Menschliche Anatomie, Zytologie, Histologie
open_access
Institut für Molekulare Infektionsbiologie
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/12846/076_Makgotlho_Plos_One.pdf
12337
2012
eng
43
2
article
1
2015-12-17
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Intracellular staphylococcus aureus: Live-in and let die
Staphylococcus aureus uses a plethora of virulence factors to accommodate a diversity of niches in its human host. Aside from the classical manifestations of S. aureus-induced diseases, the pathogen also invades and survives within mammalian host cells. The survival strategies of the pathogen are as diverse as strains or host cell types used. S. aureus is able to replicate in the phagosome or freely in the cytoplasm of its host cells. It escapes the phagosome of professional and non-professional phagocytes, subverts autophagy, induces cell death mechanisms such as apoptosis and pyronecrosis, and even can induce anti-apoptotic programs in phagocytes. The focus of this review is to present a guide to recent research outlining the variety of intracellular fates of S. aureus.
Frontiers in Cellular and Infection Microbiology
10.3389/fcimb.2012.00043
urn:nbn:de:bvb:20-opus-123374
Frontiers Cellular Infection Microbiology 2:43. doi:10.3389/fcimb.2012.00043
Martin Fraunholz
Bhanu Sinha
eng
uncontrolled
staphylococcus aureus
eng
uncontrolled
bacterial persistence
eng
uncontrolled
host cell death
eng
uncontrolled
autophagy
eng
uncontrolled
phagocytosis
eng
uncontrolled
phagosomalescape
Mikroorganismen, Pilze, Algen
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/12337/fcimb-02-00043(1).pdf
12497
2015
eng
25
10
article
1
2016-01-22
--
--
Different duration strategies of perioperative antibiotic prophylaxis in adult patients undergoing cardiac surgery: an observational study
Background
All international guidelines recommend perioperative antibiotic prophylaxis (PAB) should be routinely administered to patients undergoing cardiac surgery. However, the duration of PAB is heterogeneous and controversial.
Methods
Between 01.01.2011 and 31.12.2011, 1096 consecutive cardiac surgery patients were assigned to one of two groups receiving PAB with a second-generation cephalosporin for either 56 h (group I) or 32 h (group II). Patients’ characteristics, intraoperative data, and the in-hospital follow-up were analysed. Primary endpoint was the incidence of surgical site infection (deep and superficial sternal wound-, and vein harvesting site infection; DSWI/SSWI/VHSI). Secondary endpoints were the incidence of respiratory-, and urinary tract infection, as well as the mortality rate.
Results
615/1096 patients (56,1%) were enrolled (group I: n = 283 versus group II: n = 332). There were no significant differences with regard to patient characteristics, comorbidities, and procedure-related variables. No statistically significant differences were demonstrated concerning primary and secondary endpoints. The incidence of DSWI/SSWI/VHSI were 4/283 (1,4%), 5/283 (1,7%), and 1/283 (0,3%) in group I versus 6/332 (1,8%), 9/332 (2,7%), and 3/332 (0,9%) in group II (p = 0,76/0,59/0,63). In univariate analyses female gender, age, peripheral arterial obstructive disease, operating-time, ICU-duration, transfusion, and respiratory insufficiency were determinants for nosocomial infections (all ≤ 0,05). Subgroup analyses of these high-risk patients did not show any differences between the two regimes (all ≥ 0,05).
Conclusions
Reducing the duration of PAB from 56 h to 32 h in adult cardiac surgery patients was not associated with an increase of nosocomial infection rate, but contributes to reduce antibiotic resistance and health care costs.
Journal of Cardiothoracic Surgery
10.1186/s13019-015-0225-x
urn:nbn:de:bvb:20-opus-124977
Journal of Cardiothoracic Surgery (2015) 10:25 DOI 10.1186/s13019-015-0225-x
Khaled Hamouda
Mehmet Oezkur
Bhanu Sinha
Johannes Hain
Hannah Menkel
Marcus Leistner
Rainer Leyh
Christoph Schimmer
eng
uncontrolled
nosocomial infection
eng
uncontrolled
cardiac surgery
eng
uncontrolled
antibiotic prophylaxis
Chirurgie und verwandte medizinische Fachrichtungen
open_access
Institut für Informatik
Klinik und Poliklinik für Thorax-, Herz- u. Thorakale Gefäßchirurgie
Förderzeitraum 2015
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/12497/Schimmer_s13019-015-0225-x.pdf
22696
2019
eng
326-336
3
37
article
1
2021-02-24
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Clonal Clusters and Virulence Factors of Methicillin-Resistant \(Staphylococcus\) \(Aureus\): Evidence for Community-Acquired Methicillin-Resistant \(Staphylococcus\) \(Aureus\) Infiltration into Hospital Settings in Chennai, South India
Background and Objective: Staphylococcus aureus is one of the major pathogens of nosocomial infections as wells as community-acquired (CA) infections worldwide. So far, large-scale comprehensive molecular and epidemiological characterisation of S. aureus from very diverse settings has not been carried out in India. The objective of this study is to evaluate the molecular, epidemiological and virulence characteristics of S. aureus in both community and hospital settings in Chennai, southern India. Methods: S. aureus isolates were obtained from four different groups (a) healthy individuals from closed community settings, (b) inpatients from hospitals, (c) outpatients from hospitals, representing isolates of hospital-community interface and (d) HIV-infected patients to define isolates associated with the immunocompromised. Antibiotic susceptibility testing, multiplex polymerase chain reactions for detection of virulence and resistance determinants, molecular typing including Staphylococcal cassette chromosome mec (SCCmec) and agr typing, were carried out. Sequencing-based typing was done using spa and multilocus sequence typing (MLST) methods. Clonal complexes (CC) of hospital and CA methicillin-resistant S. aureus (MRSA) were identified and compared for virulence and resistance.
Results and Conclusion: A total of 769 isolates of S. aureus isolates were studied. The prevalence of MRSA was found to be 7.17%, 81.67%, 58.33% and 22.85% for groups a, b, c and d, respectively. Of the four SCCmec types (I, III, IV and V) detected, SCCmec V was found to be predominant. Panton-Valentine leucocidin toxin genes were detected among MRSA isolates harbouring SCCmec IV and V. A total of 78 spa types were detected, t657 being the most prevalent. 13 MLST types belonging to 9 CC were detected. CC1 (ST-772, ST-1) and CC8 (ST238, ST368 and ST1208) were found to be predominant among MRSA. CA-MRSA isolates with SCCmec IV and V were isolated from all study groups including hospitalised patients and were found to be similar by molecular tools. This shows that CA MRSA has probably infiltrated into the hospital settings.
Indian Journal of Medical Microbiology
10.4103/ijmm.IJMM_18_271
urn:nbn:de:bvb:20-opus-226963
publish
Indian Journal of Medical Microbiology, Volume 37, Issue 3, 2019, Pages 326-336. doi:10.4103/ijmm.IJMM_18_271
CC BY-NC-ND: Creative-Commons-Lizenz: Namensnennung, Nicht kommerziell, Keine Bearbeitungen 4.0 International
Nagarajan Abimannan
G. Sumathi
O. R. Krishnarajasekhar
Bhanu Sinha
Padma Krishnan
eng
uncontrolled
Community-acquired methicillin-resistant Staphylococcus aureus
eng
uncontrolled
HIV
eng
uncontrolled
hospital-acquired methicillin-resistant Staphylococcus aureus
eng
uncontrolled
innate immune evasions
eng
uncontrolled
MLST
eng
uncontrolled
microbial surface component recognising adhesive matrix molecules
eng
uncontrolled
spa typing
eng
uncontrolled
ST 772
eng
uncontrolled
Inducible Clindamycin Resistance
eng
uncontrolled
Valentine Leukocidin Genes
eng
uncontrolled
Multiplex PCR
eng
uncontrolled
Nasal Carriage
eng
uncontrolled
Colonization
eng
uncontrolled
Prevalence
eng
uncontrolled
Emergence
eng
uncontrolled
Skin
Medizin und Gesundheit
open_access
Institut für Hygiene und Mikrobiologie
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/22696/clonal_clusters_and_virulence.pdf