6365
2011
eng
article
1
2013-01-16
--
--
Chemically Induced Photoswitching of Fluorescent Probes - A General Concept for Super-Resolution Microscopy
We review fluorescent probes that can be photoswitched or photoactivated and are suited for single-molecule localization based super-resolution microscopy. We exploit the underlying photochemical mechanisms that allow photoswitching of many synthetic organic fluorophores in the presence of reducing agents, and study the impact of these on the photoswitching properties of various photoactivatable or photoconvertible fluorescent proteins. We have identified mEos2 as a fluorescent protein that exhibits reversible photoswitching under various imaging buffer conditions and present strategies to characterize reversible photoswitching. Finally, we discuss opportunities to combine fluorescent proteins with organic fluorophores for dual-color photoswitching microscopy.
urn:nbn:de:bvb:20-opus-74896
7489
In: Molecules (2011) 16, 3106-3118; doi:10.3390/molecules16043106
Ulrike Endesfelder
Sebastian Malkusch
Benjamin Flottmann
Justine Mondry
Piotr Liguzinski
Peter J. Verveer
Mike Heilemann
deu
swd
Super-Resolution Microscopy
eng
uncontrolled
photoswitchable organic fluorophores
eng
uncontrolled
fluorescent proteins
eng
uncontrolled
super-resolution
eng
uncontrolled
PALM
eng
uncontrolled
dSTORM
Biowissenschaften; Biologie
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/6365/Heilemann_molecules_16_03106_v2.pdf
13408
2011
eng
3106-3118
4
16
article
1
2016-05-27
--
--
Chemically Induced Photoswitching of Fluorescent Probes - A General Concept for Super-Resolution Microscopy
We review fluorescent probes that can be photoswitched or photoactivated and are suited for single-molecule localization based super-resolution microscopy. We exploit the underlying photochemical mechanisms that allow photoswitching of many synthetic organic fluorophores in the presence of reducing agents, and study the impact of these on the photoswitching properties of various photoactivatable or photoconvertible fluorescent proteins. We have identified mEos2 as a fluorescent protein that exhibits reversible photoswitching under various imaging buffer conditions and present strategies to characterize reversible photoswitching. Finally, we discuss opportunities to combine fluorescent proteins with organic fluorophores for dual-color photoswitching microscopy.
Molecules
10.3390/molecules16043106
urn:nbn:de:bvb:20-opus-134080
Molecules 2011, 16, 3106-3118; doi:10.3390/molecules16043106
Ulrike Endesfelder
Sebastian Malkusch
Benjamin Flottmann
Justine Mondry
Piotr Liguzinski
Peter J. Verveer
Mike Heilemann
eng
uncontrolled
Photoactivated localization microscopy
eng
uncontrolled
Fusion proteins
eng
uncontrolled
Molecules
eng
uncontrolled
Patterns
eng
uncontrolled
Switch
eng
uncontrolled
Limit
eng
uncontrolled
Time
eng
uncontrolled
photoswitchable organic fluorophores
eng
uncontrolled
fluorescent proteins
eng
uncontrolled
super-resolution
eng
uncontrolled
PALM
eng
uncontrolled
dSTORM
Biowissenschaften; Biologie
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/13408/024_Endesfelder_molecules.pdf