11288
2014
eng
article
1
2015-05-08
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Actinomycetes from Red Sea Sponges: Sources for Chemical and Phylogenetic Diversity
The diversity of actinomycetes associated with marine sponges collected off Fsar Reef (Saudi Arabia) was investigated in the present study. Forty-seven actinomycetes were cultivated and phylogenetically identified based on 16S rRNA gene sequencing and were assigned to 10 different actinomycete genera. Eight putatively novel species belonging to genera Kocuria, Mycobacterium, Nocardia, and Rhodococcus were identified based on sequence similarity values below 98.2% to other 16S rRNA gene sequences available in the NCBI database. PCR-based screening for biosynthetic genes including type I and type II polyketide synthases (PKS-I, PKS-II) as well as nonribosomal peptide synthetases (NRPS) showed that 20 actinomycete isolates encoded each at least one type of biosynthetic gene. The organic extracts of nine isolates displayed bioactivity against at least one of the test pathogens, which were Gram-positive and Gram-negative bacteria, fungi, human parasites, as well as in a West Nile Virus protease enzymatic assay. These results emphasize that marine sponges are a prolific resource for novel bioactive actinomycetes with potential for drug discovery.
10.3390/md12052771
urn:nbn:de:bvb:20-opus-112882
Marine Drugs 2014, 12, 2771-2789; doi:10.3390/md12052771
Usama Ramadan Abdelmohsen
Chen Yang
Hannes Horn
Dina Hajjar
Timothy Ravasi
Ute Hentschel
eng
uncontrolled
PKS I
deu
swd
Meeresschwämme
eng
uncontrolled
PKS II
eng
uncontrolled
NRPS
eng
uncontrolled
Red sea
eng
uncontrolled
sponges
eng
uncontrolled
actinomycetes
eng
uncontrolled
bioactivity
Wirbellose des Meeres und der Meeresküste
open_access
Julius-von-Sachs-Institut für Biowissenschaften
Förderzeitraum 2014
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/11288/090_Hentschel_MarineDrugs.pdf
11277
2014
eng
article
1
2015-05-07
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Draft Genome Sequence of the Antitrypanosomally Active Sponge-Associated Bacterium Actinokineospora sp. Strain EG49
The marine sponge-associated bacterium Actinokineospora sp. strain EG49 produces the antitrypanosomal angucycline-like compound actinosporin A. The draft genome of Actinokineospora sp. EG49 has a size of 7.5 megabases and a GC content of 72.8% and contains 6,629 protein-coding sequences (CDS). antiSMASH predicted 996 genes residing in 36 secondary metabolite gene clusters.
10.1128/genomeA.00160-14
urn:nbn:de:bvb:20-opus-112776
Genome Announcements 2(2):e00160-14. doi:10.1128/genomeA.00160-14
Janno Harjes
Taewoo Ryu
Usama Ramadan Abdelmohsen
Lucas Moitinho-Silva
Hannes Horn
Timothy Ravasi
Ute Hentschel
deu
swd
Strahlenpilze
Biowissenschaften; Biologie
open_access
Julius-von-Sachs-Institut für Biowissenschaften
Förderzeitraum 2014
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/11277/075_Hentschel_GenomeA.pdf