2715
1991
eng
article
1
2009-01-20
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Spontaneous assembly of pore complex-containing membranes ("Annulate lamellae") in Xenopus egg extract in the absence of chromatin
No abstract available
urn:nbn:de:bvb:20-opus-32797
3279
In: The Journal of Cell Biology (1991) 112, 6, 1073-1082.
Marie-Christine Dabauvalle
Karin Loos
Hilde Merkert
Ulrich Scheer
Biowissenschaften; Biologie
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/2715/Scheer_Spontaneous.pdf
3312
1991
eng
article
1
2009-11-10
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Localization of nucleolar chromatin by immunocytochemistry and in situ hybridization at the electron microscopic level
Nucleoli are the morphological expression of the activity of a defined set of chromosomal segments bearing rRNA genes. The topological distribution and composition of the intranucleolar chromatin as well as the definition of nucleolar structures in which enzymes of the rDNA transcription machinery reside have been investigated in mammalian cells by various immunogold labelling approaches at the ultrastructural level. The precise intranucleolar location of rRNA genes has been further specified by electron microscopic in situ hybridization with a non-autoradiographic procedure. Our results indicate that the fibrillar centers are the sole nucleolar structures where rDNA, core histones, RNA polymerase I and DNA to po isomerase I are located together. Taking into account the potential value and limitations of immunoelectron microscopic techniques, we propose that transcription of the rRNA genes takes place within the confines of the fibrillar centers, probably close to the boundary regions to the surrounding dense fibrillar component.
urn:nbn:de:bvb:20-opus-39289
3928
In: Electron Microsc. Rev., 1991, 4, 85-110.
Marc Thiry
Ulrich Scheer
Guy Goessens
Biowissenschaften; Biologie
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/3312/Scheer_Localization_nucleolar.pdf