4512
1991
eng
article
1
2010-12-14
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The transcriptional transactivator of human foamy virus maps to the bel 1 genomic region
The human foamy virus (HFV) genome possesses three open reading frames (bel I, 2, and 3) located between env and the 3' long terminal repeat. By analogy to other human retroviruses this region was selected as the most Iikely candidate to encode the viral transactivator. ResuIts presented here confirmed this and showed further that a deletion introduced only into the bell open reading frame of a plasmid derived from an infectious molecular clone of HFV abolished transactivation. In contrast, deletions in bel 2 and bel 3 had only minor effects on the ability to transactivate. The role of the bel I genomic region as a transactivator was further investigated by eukaryotic expression of a genome fragment of HFV spanning the bel I open reading frame. A construct expressing bell under control of a heterologous promoter was found to transactivate the HFV long terminal repeat in a dose-dependent fashion. Furthermore, it is shown that the U3 region of the HFV long terminal repeat is sufficient to respond to the HFV transactivator.
urn:nbn:de:bvb:20-opus-47342
4734
In: Proceedings of the National Academy of Sciences of the United States of America (1991) 3, 88, 941-945.
Deutsches Urheberrecht
Axel Rethwilm
Otto Erlwein
Gerald Baunach
Bernd Mauerer
Volker ter Meulen
deu
swd
Virologie
Medizin und Gesundheit
open_access
Institut für Virologie und Immunbiologie
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/4512/Rethwilm_transcriptional_transactivator.pdf
5128
1990
eng
article
1
2011-09-17
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Infectious DNA of the human spumaretrovirus
An infectious molecular clone (pHSRV) of the human Spumaretrovirus (HSRV) was constructed using viral DNA and cDNA clones. The infectivity of pHSRV was proven by transfection of cell cultures and subsequent infection of susceptible cultures with cell free transfection derlved virus. pHSRV derived virus produced foamy virus typical cytopathic effects in susceptible cultures. lnfected cells could be stained specifically with foamy virus antisera by means of indirect immunofluorescence. Radiolmmunoprecipltatlon revealed the presence of characteristic HSRV structural proteins in pHSRV infected cultures. By cotransfection of pHSRV and an indicator plasmid it was found that pHSRV is able to transactivate the viral L TR. Viral transcripts were found to be approximately 200 bases Ionger in pHSRV infected cultures compared to wildtype infected cultures. This difference is most likely due to an Insertion of DNA of non-viral origin ln the U3 region of the 3'L TR of the infectious clone.
urn:nbn:de:bvb:20-opus-61495
6149
In: Nucleic Acids Research (1990) 18, 4, 733-8
Deutsches Urheberrecht
Axel Rethwilm
Gerald Baunach
Kai O. Netzer
Bernd Maurer
Bettina Borisch
V.olker ter Meulen
deu
swd
Virologie
Medizin und Gesundheit
open_access
Institut für Virologie und Immunbiologie
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/5128/Rethwilm22.pdf
5115
1994
eng
article
1
2011-09-17
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Reactivity of primate sera to foamy virus Gag and Bet proteins
In order to establish criteria for the Serodiagnosis of foamy virus infections we investigated the extent to which sera from iofected individuals of human and primate origin react with structural and non-structural virus proteins in immunoblot assays. Using lysates from infected cells as the source of virus antigen, antibodies were preferentially detected against the Gag proteins and the non-structural Bet protein. Both the Gag precursor molecules of 70 and 74K apparent M\(_r\) and the cytoplasmic 60K M\(_r\) Bet protein were found to be phosphorylated, the latter being synthesized in large amounts in infected cells. Rahbit antiserum raised against recombinant human foamy virus (HFV) Gag major capsid protein cross-reacted with foamy viruses of chimpanzee, gorilla, orang-utan, rhesus monkey and Mrican green monkey origin. This was reßected by a broad cross-reactivity of the respective monkey sera to the Gag proteins of the various foamy virus isolates. Cross-reactivity of antisera against the Bet protein was restricted to viruses from man and the great apes. Recombinant Gag and Bet proteins expressed in prokaryotes or in insect cells were readily recognized by foamy virus-positive primate sera. Screening serum samples from chimpanzees with HFV Gag and Bet proteins expressed by recombinant baculoviruses revealed that 18 out of 35 (52%) were positive for Gag antibodies. Of these, 13 (72 o/o) showed antiborlies against the Bet protein, indicating that Bet antigen is of value in sero1ogical screening for foamy virus infections.
urn:nbn:de:bvb:20-opus-61366
6136
Journal of General Virology (1994) 75, 10, 2635-2644
Deutsches Urheberrecht
Heidi Hahn
Gerald Baunach
Sandra Bräutigam
Ayalew Mergia
Dieter Neumann-Haefelin
Muthiah D. Daniel
Myra O. McClure
Axel Rethwilm
deu
swd
Virologie
Medizin und Gesundheit
open_access
Institut für Virologie und Immunbiologie
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/5115/Rethwilm02.pdf
5118
1993
eng
article
1
2011-09-17
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Functional analysis of human foamy virus accessory reading frames
No abstract available
urn:nbn:de:bvb:20-opus-61398
6139
In: Journal of Virology (1993) 67, 9, 5411-5418
Deutsches Urheberrecht
Gerald Baunach
Bernd Maurer
Heidi Hahn
Manuela Kranz
Axel Rethwilm
deu
swd
Virologie
Medizin und Gesundheit
open_access
Institut für Virologie und Immunbiologie
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/5118/Rethwilm07.pdf
7279
1990
eng
conferenceobject
1
2013-08-21
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Transactivation of HIV by human spumaretrovirus
To study the activation of HIV by human spumaretrovirus (HSRV) the long terminal repeats (LTRs) of HSRV, HIVl and HIV2 were examined with respect to their ability to function as transcriptional promoters in virus infected and uninfected cells. Transient transfections using plasmids in which the L TRs of the three viruses were coupled to the bacterial chloramphenicol acetyltransferase (CA T) gene revealed (i) the level of cat gene expression directed by the HSRV LTR was markedly increased in HSRV infected cells compared to uninfected cells, (ii) cat gene expression driven by the HIV1 LTR, but not by the HIV2 LTR could be enhanced upon HSRV infection, whereas (iii) neither in HIV1 nor in HIV2 infected cells an effect on HSRV LTR driven cat geneexpression was detected.
8025
urn:nbn:de:bvb:20-opus-86436
In: Progress in aids research in the Federal Republic of Germany / ed. Marianna Schauzu. - München: MMV, Medizin-Verl., 1990. - S. 31-33. - ISBN 3-8208-1140-0
Deutsches Urheberrecht
Axel Rethwilm
Gerald Baunach
Kazuyasu Mori
Volker ter Meulen
deu
swd
HIV
Medizin und Gesundheit
open_access
Institut für Virologie und Immunbiologie
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/7279/Rethwilm_7279.pdf