12308
2012
eng
69-96
6
article
1
2015-12-14
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--
Transcriptome analysis in tardigrade species reveals specific molecular pathways for stress adaptations
Tardigrades have unique stress-adaptations that allow them to survive extremes of cold, heat, radiation and vacuum. To study this, encoded protein clusters and pathways from an ongoing transcriptome study on the tardigrade \(Milnesium\) \(tardigradum\) were analyzed using bioinformatics tools and compared to expressed sequence tags (ESTs) from \(Hypsibius\) \(dujardini\), revealing major pathways involved in resistance against extreme environmental conditions. ESTs are available on the Tardigrade Workbench along with software and databank updates. Our analysis reveals that RNA stability motifs for \(M.\) \(tardigradum\) are different from typical motifs known from higher animals. \(M.\) \(tardigradum\) and \(H.\) \(dujardini\) protein clusters and conserved domains imply metabolic storage pathways for glycogen, glycolipids and specific secondary metabolism as well as stress response pathways (including heat shock proteins, bmh2, and specific repair pathways). Redox-, DNA-, stress- and protein protection pathways complement specific repair capabilities to achieve the strong robustness of \(M.\) \(tardigradum\). These pathways are partly conserved in other animals and their manipulation could boost stress adaptation even in human cells. However, the unique combination of resistance and repair pathways make tardigrades and \(M.\) \(tardigradum\) in particular so highly stress resistant.
Bioinformatics and biology insights
PMC3342025
10.4137/BBI.S9150
urn:nbn:de:bvb:20-opus-123089
This is an open access article. Unrestricted non-commercial use is permitted provided the original work is properly cited.
Bioinformatics and Biology Insights 2012:6 69–96. doi: 10.4137/BBI.S9150
Frank Förster
Daniela Beisser
Markus A. Grohme
Chunguang Liang
Brahim Mali
Alexander Matthias Siegl
Julia C. Engelmann
Alexander V. Shkumatov
Elham Schokraie
Tobias Müller
Martina Schnölzer
Ralph O. Schill
Marcus Frohme
Thomas Dandekar
eng
uncontrolled
RNA
eng
uncontrolled
expressed sequence tag
eng
uncontrolled
cluster
eng
uncontrolled
protein familiy
eng
uncontrolled
adaption
eng
uncontrolled
tardigrada
eng
uncontrolled
transcriptome
Biowissenschaften; Biologie
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/12308/f_3147-BBI-Transcriptome-Analysis-in-Tardigrade-Species-Reveals-Specific-Molecula.pdf_4261.pdf
13444
2012
eng
e45682
9
7
article
1
2016-06-06
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Comparative proteome analysis of Milnesium tardigradum in early embryonic state versus adults in active and anhydrobiotic state
Tardigrades have fascinated researchers for more than 300 years because of their extraordinary capability to undergo cryptobiosis and survive extreme environmental conditions. However, the survival mechanisms of tardigrades are still poorly understood mainly due to the absence of detailed knowledge about the proteome and genome of these organisms. Our study was intended to provide a basis for the functional characterization of expressed proteins in different states of tardigrades. High-throughput, high-accuracy proteomics in combination with a newly developed tardigrade specific protein database resulted in the identification of more than 3000 proteins in three different states: early embryonic state and adult animals in active and anhydrobiotic state. This comprehensive proteome resource includes protein families such as chaperones, antioxidants, ribosomal proteins, cytoskeletal proteins, transporters, protein channels, nutrient reservoirs, and developmental proteins. A comparative analysis of protein families in the different states was performed by calculating the exponentially modified protein abundance index which classifies proteins in major and minor components. This is the first step to analyzing the proteins involved in early embryonic development, and furthermore proteins which might play an important role in the transition into the anhydrobiotic state.
PLoS One
10.1371/journal.pone.0045682
urn:nbn:de:bvb:20-opus-134447
PLoS ONE 7(9): e45682. doi:10.1371/journal.pone.0045682
Elham Schokraie
Uwe Warnken
Agnes Hotz-Wagenblatt
Markus A. Grohme
Steffen Hengherr
Frank Förster
Ralph O. Schill
Marcus Frohme
Thomas Dandekar
Martina Schnölzer
eng
uncontrolled
life-span regulation
eng
uncontrolled
genes
eng
uncontrolled
Yolk protein
eng
uncontrolled
water stress
eng
uncontrolled
expression
eng
uncontrolled
tolerance
eng
uncontrolled
richtersius coronifer
eng
uncontrolled
superoxide-dismutase
eng
uncontrolled
caenorhabditis elegans
eng
uncontrolled
arabidopsis thaliana
eng
uncontrolled
vitellogenin
Datenverarbeitung; Informatik
Biowissenschaften; Biologie
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/13444/Schokraie_PLoSOne.pdf