20174
2019
eng
10788–10800
9
2019
article
1
2020-03-16
--
--
Linking pollen foraging of megachilid bees to their nest bacterial microbiota
Solitary bees build their nests by modifying the interior of natural cavities, and they provision them with food by importing collected pollen. As a result, the microbiota of the solitary bee nests may be highly dependent on introduced materials. In order to investigate how the collected pollen is associated with the nest microbiota, we used metabarcoding of the ITS2 rDNA and the 16S rDNA to simultaneously characterize the pollen composition and the bacterial communities of 100 solitary bee nest chambers belonging to seven megachilid species. We found a weak correlation between bacterial and pollen alpha diversity and significant associations between the composition of pollen and that of the nest microbiota, contributing to the understanding of the link between foraging and bacteria acquisition for solitary bees. Since solitary bees cannot establish bacterial transmission routes through eusociality, this link could be essential for obtaining bacterial symbionts for this group of valuable pollinators.
Ecology and Evolution
10.1002/ece3.5599
00
urn:nbn:de:bvb:20-opus-201749
Ecology and Evolution. 2019;9:10788–10800. doi:10.1002/ece3.5599
false
true
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Anna Voulgari‐Kokota
Markus J. Ankenbrand
Gudrun Grimmer
Ingolf Steffan‐Dewenter
Alexander Keller
eng
uncontrolled
foraging patterns
eng
uncontrolled
nest microbiota
eng
uncontrolled
plant–microbe–pollinator triangle
eng
uncontrolled
pollination network
eng
uncontrolled
solitary bees
eng
uncontrolled
wild bees
Arthropoden (Gliederfüßer)
open_access
Theodor-Boveri-Institut für Biowissenschaften
Förderzeitraum 2019
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/20174/Voulgari-Kokota_ece3.5599.pdf
12573
2015
eng
20
15
article
1
2016-01-27
--
--
Increased efficiency in identifying mixed pollen samples by meta-barcoding with a dual-indexing approach
Background
Meta-barcoding of mixed pollen samples constitutes a suitable alternative to conventional pollen identification via light microscopy. Current approaches however have limitations in practicability due to low sample throughput and/or inefficient processing methods, e.g. separate steps for amplification and sample indexing.
Results
We thus developed a new primer-adapter design for high throughput sequencing with the Illumina technology that remedies these issues. It uses a dual-indexing strategy, where sample-specific combinations of forward and reverse identifiers attached to the barcode marker allow high sample throughput with a single sequencing run. It does not require further adapter ligation steps after amplification. We applied this protocol to 384 pollen samples collected by solitary bees and sequenced all samples together on a single Illumina MiSeq v2 flow cell. According to rarefaction curves, 2,000–3,000 high quality reads per sample were sufficient to assess the complete diversity of 95% of the samples. We were able to detect 650 different plant taxa in total, of which 95% were classified at the species level. Together with the laboratory protocol, we also present an update of the reference database used by the classifier software, which increases the total number of covered global plant species included in the database from 37,403 to 72,325 (93% increase).
Conclusions
This study thus offers improvements for the laboratory and bioinformatical workflow to existing approaches regarding data quantity and quality as well as processing effort and cost-effectiveness. Although only tested for pollen samples, it is furthermore applicable to other research questions requiring plant identification in mixed and challenging samples.
BMC Ecology
10.1186/s12898-015-0051-y
urn:nbn:de:bvb:20-opus-125730
D-1221-2009
BMC Ecology (2015) 15:20 DOI 10.1186/s12898-015-0051-y
Wiebke Sickel
Markus J. Ankenbrand
Gudrun Grimmer
Andrea Holzschuh
Stephan Härtel
Jonathan Lanzen
Ingolf Steffan-Dewenter
Alexander Keller
eng
uncontrolled
pollination ecology
eng
uncontrolled
next generation sequencing
eng
uncontrolled
ITS2
eng
uncontrolled
illumina MiSeq platform
eng
uncontrolled
high throughput sequencing
eng
uncontrolled
DNA barcoding
eng
uncontrolled
NGS
eng
uncontrolled
osmia
eng
uncontrolled
palynolog
Medizin und Gesundheit
open_access
Theodor-Boveri-Institut für Biowissenschaften
Förderzeitraum 2015
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/12573/Keller_s12898-015-0051-y.pdf
9730
2013
eng
article
1
--
--
--
Diverse Microbiota Identified in Whole Intact Nest Chambers of the Red Mason Bee Osmia bicornis (Linnaeus 1758)
Microbial activity is known to have profound impact on bee ecology and physiology, both by beneficial and pathogenic effects. Most information about such associations is available for colony-building organisms, and especially the honey bee. There, active manipulations through worker bees result in a restricted diversity of microbes present within the colony environment. Microbial diversity in solitary bee nests remains unstudied, although their larvae face a very different situation compared with social bees by growing up in isolated compartments. Here, we assessed the microbiota present in nests and pre-adults of Osmia bicornis, the red mason bee, by culture-independent pyrosequencing. We found high bacterial diversity not comparable with honey bee colonies. We identified a variety of bacteria potentially with positive or negative interactions for bee larvae. However, most of the other diverse bacteria present in the nests seem to originate from environmental sources through incorporated nest building material and stored pollen. This diversity of microorganisms may cause severe larval mortality and require specific physiological or symbiotic adaptations against microbial threats. They may however also profit from such a diverse environment through gain of mutualistic partners. We conclude that further studies of microbiota interaction in solitary bees will improve the understanding of fitness components and populations dynamics.
PLoS One
10.1371/journal.pone.0078296
urn:nbn:de:bvb:20-opus-97305
DNA Analytics Core Facility, Biocenter, University of Wuerzburg, Wuerzburg, Germany
In: PLoS One (2013) 8: 10, doi:10.1371/journal.pone.0078296
Alexander Keller
Gudrun Grimmer
Ingolf Steffan-Dewenter
eng
uncontrolled
bacteria
eng
uncontrolled
bacterial pathogens
eng
uncontrolled
bees
eng
uncontrolled
gut bacteria
eng
uncontrolled
honey bees
eng
uncontrolled
larvae
eng
uncontrolled
Pollen
eng
uncontrolled
Polymerase chain reaction
Tiere (Zoologie)
open_access
Theodor-Boveri-Institut für Biowissenschaften
Förderzeitraum 2013
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/9730/Keller_journal.pone.0078296.pdf
23885
2021
eng
7700
7712
12
11
article
1
--
--
--
Cryptic species and hidden ecological interactions of halictine bees along an elevational gradient
Changes of abiotic and biotic conditions along elevational gradients represent serious challenges to organisms which may promote the turnover of species, traits and biotic interaction partners. Here, we used molecular methods to study cuticular hydrocarbon (CHC) profiles, biotic interactions and phylogenetic relationships of halictid bees of the genus Lasioglossum along a 2,900 m elevational gradient at Mt. Kilimanjaro, Tanzania. We detected a strong species turnover of morphologically indistinguishable taxa with phylogenetically clustered cryptic species at high elevations, changes in CHC profiles, pollen resource diversity, and a turnover in the gut and body surface microbiome of bees. At high elevations, increased proportions of saturated compounds in CHC profiles indicate physiological adaptations to prevent desiccation. More specialized diets with higher proportions of low‐quality Asteraceae pollen imply constraints in the availability of food resources. Interactive effects of climatic conditions on gut and surface microbiomes, CHC profiles, and pollen diet suggest complex feedbacks among abiotic conditions, ecological interactions, physiological adaptations, and phylogenetic constraints as drivers of halictid bee communities at Mt. Kilimanjaro.
Ecology and Evolution
10.1002/ece3.7605
urn:nbn:de:bvb:20-opus-238853
2021-06-01T10:32:58+00:00
sword
swordwue
attachment; filename=deposit.zip
7a8d14f0ed9d1f1e685df34f4b877b5c
Ecology and Evolution 2021, 11(12):7700-7712. DOI: 10.1002/ece3.7605
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Antonia V. Mayr
Alexander Keller
Marcell K. Peters
Gudrun Grimmer
Beate Krischke
Mareen Geyer
Thomas Schmitt
Ingolf Steffan‐Dewenter
eng
uncontrolled
COI
eng
uncontrolled
cuticular chemistry
eng
uncontrolled
elevational gradient
eng
uncontrolled
Halictidae
eng
uncontrolled
microbiome metabarcoding
eng
uncontrolled
pollen metabarcoding
Biowissenschaften; Biologie
open_access
Theodor-Boveri-Institut für Biowissenschaften
Import
Förderzeitraum 2021
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/23885/Mayr_Ecology.pdf