14092
2011
eng
e25409
10
6
article
1
2016-11-25
--
--
Magnetic Resonance Imaging of Tumors Colonized with Bacterial Ferritin-Expressing \(Escherichia\) \(coli\)
Background:
Recent studies have shown that human ferritin can be used as a reporter of gene expression for magnetic resonance imaging (MRI). Bacteria also encode three classes of ferritin-type molecules with iron accumulation properties.
Methods and Findings:
Here, we investigated whether these bacterial ferritins can also be used as MRI reporter genes and which of the bacterial ferritins is the most suitable reporter. Bacterial ferritins were overexpressed in probiotic E. coli Nissle 1917. Cultures of these bacteria were analyzed and those generating highest MRI contrast were further investigated in tumor bearing mice. Among members of three classes of bacterial ferritin tested, bacterioferritin showed the most promise as a reporter gene. Although all three proteins accumulated similar amounts of iron when overexpressed individually, bacterioferritin showed the highest contrast change. By site-directed mutagenesis we also show that the heme iron, a unique part of the bacterioferritin molecule, is not critical for MRI contrast change. Tumor-specific induction of bacterioferritin-expression in colonized tumors resulted in contrast changes within the bacteria-colonized tumors.
Conclusions:
Our data suggest that colonization and gene expression by live vectors expressing bacterioferritin can be monitored by MRI due to contrast changes.
PLoS ONE
10.1371/journal.pone.0025409
urn:nbn:de:bvb:20-opus-140920
PLoS ONE 6(10): e25409. doi:10.1371/journal.pone.0025409
Philip J. Hill
Jochen Stritzker
Miriam Scadeng
Ulrike Geissinger
Daniel Haddad
Thomas C. Basse-Lüsebrink
Uwe Gbureck
Peter Jakob
Aladar A. Szalay
eng
uncontrolled
Blood-brain barrier
eng
uncontrolled
Gene-expression
eng
uncontrolled
Salmonella-typhimurium
eng
uncontrolled
Sugar-transport
eng
uncontrolled
Breast-tumors
eng
uncontrolled
MRI reporter
eng
uncontrolled
Iron-uptake
eng
uncontrolled
Proteins
eng
uncontrolled
Therapy
eng
uncontrolled
Mice
Krankheiten
open_access
Physikalisches Institut
Institut für Molekulare Infektionsbiologie
Rudolf-Virchow-Zentrum
Lehrstuhl für Biochemie
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/14092/085_Hill_PLOSE-ONE.PDF
6402
2011
eng
article
1
2013-02-05
--
--
Magnetic Resonance Imaging of Tumors Colonized with Bacterial Ferritin-Expressing Escherichia coli
Background: Recent studies have shown that human ferritin can be used as a reporter of gene expression for magnetic resonance imaging (MRI). Bacteria also encode three classes of ferritin-type molecules with iron accumulation properties. Methods and Findings: Here, we investigated whether these bacterial ferritins can also be used as MRI reporter genes and which of the bacterial ferritins is the most suitable reporter. Bacterial ferritins were overexpressed in probiotic E. coli Nissle 1917. Cultures of these bacteria were analyzed and those generating highest MRI contrast were further investigated in tumor bearing mice. Among members of three classes of bacterial ferritin tested, bacterioferritin showed the most promise as a reporter gene. Although all three proteins accumulated similar amounts of iron when overexpressed individually, bacterioferritin showed the highest contrast change. By site-directed mutagenesis we also show that the heme iron, a unique part of the bacterioferritin molecule, is not critical for MRI contrast change. Tumor-specific induction of bacterioferritin-expression in colonized tumors resulted in contrast changes within the bacteria-colonized tumors. Conclusions: Our data suggest that colonization and gene expression by live vectors expressing bacterioferritin can be monitored by MRI due to contrast changes
urn:nbn:de:bvb:20-opus-75789
7578
In: PLoS ONE (2011) 6(10): e25409. doi:10.1371/journal.pone.0025409
Aladar A. Szalay
Philip J. Hill
Jochen Stritzker
Miriam Scadeng
Ulrike Geissinger
Daniel Haddad
Thomas C. Basse-Lüsebrink
Uwe Gbureck
Peter Jakob
deu
swd
Escherichia coli
Biowissenschaften; Biologie
open_access
Rudolf-Virchow-Zentrum
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/6402/Szalay_journal.pone.0025409.pdf