12116
2014
eng
755-66
2
65
article
1
2015-10-28
--
--
The Venus flytrap attracts insects by the release of volatile organic compounds
Does Dionaea muscipula, the Venus flytrap, use a particular mechanism to attract animal prey? This question was raised by Charles Darwin 140 years ago, but it remains unanswered. This study tested the hypothesis that Dionaea releases volatile organic compounds (VOCs) to allure prey insects. For this purpose, olfactory choice bioassays were performed to elucidate if Dionaea attracts Drosophila melanogaster. The VOCs emitted by the plant were further analysed by GC-MS and proton transfer reaction-mass spectrometry (PTR-MS). The bioassays documented that Drosophila was strongly attracted by the carnivorous plant. Over 60 VOCs, including terpenes, benzenoids, and aliphatics, were emitted by Dionaea, predominantly in the light. This work further tested whether attraction of animal prey is affected by the nutritional status of the plant. For this purpose, Dionaea plants were fed with insect biomass to improve plant N status. However, although such feeding altered the VOC emission pattern by reducing terpene release, the attraction of Drosophila was not affected. From these results it is concluded that Dionaea attracts insects on the basis of food smell mimicry because the scent released has strong similarity to the bouquet of fruits and plant flowers. Such a volatile blend is emitted to attract insects searching for food to visit the deadly capture organ of the Venus flytrap.
Journal of Experimental Botany
10.1093/jxb/ert455
24420576
urn:nbn:de:bvb:20-opus-121161
Journal of Experimental Botany, Vol. 65, No. 2, pp. 755–766, 2014 doi:10.1093/jxb/ert455
Jürgen Kreuzwieser
Ursel Scheerer
Jörg Kruse
Tim Burzlaff
Anne Honsel
Saleh Alfarraj
Palmen Georgiev
Jörg-Peter Schnitzler
Andrea Ghirardo
Ines Kreuzer
Rainer Hedrich
Heinz Rennenberg
eng
uncontrolled
carnivorus plants
eng
uncontrolled
dionaea muscipula
eng
uncontrolled
drosophila melanogaster
eng
uncontrolled
VOC emissions
eng
uncontrolled
nitrogen status
eng
uncontrolled
olfactory bioassay
eng
uncontrolled
plant-animal interaction
Biowissenschaften; Biologie
open_access
Julius-von-Sachs-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/12116/025_Kreuzwieser_Journal_of_Experimental_Botany.pdf
12805
2016
eng
286–295
3
26
article
1
2016-02-26
--
--
The Venus Flytrap Dionaea muscipula Counts Prey-Induced Action Potentials to Induce Sodium Uptake
Carnivorous plants, such as the Venus flytrap (Dionaea muscipula), depend on an animal diet when grown in nutrient-poor soils. When an insect visits the trap and tilts the mechanosensors on the inner surface, action potentials (APs) are fired. After a moving object elicits two APs, the trap snaps shut, encaging the victim. Panicking preys repeatedly touch the trigger hairs over the subsequent hours, leading to a hermetically closed trap, which via the gland-based endocrine system is flooded by a prey-decomposing acidic enzyme cocktail. Here, we asked the question as to how many times trigger hairs have to be stimulated (e.g., now many APs are required) for the flytrap to recognize an encaged object as potential food, thus making it worthwhile activating the glands. By applying a series of trigger-hair stimulations, we found that the touch hormone jasmonic acid (JA) signaling pathway is activated after the second stimulus, while more than three APs are required to trigger an expression of genes encoding prey-degrading hydrolases, and that this expression is proportional to the number of mechanical stimulations. A decomposing animal contains a sodium load, and we have found that these sodium ions enter the capture organ via glands. We identified a flytrap sodium channel DmHKT1 as responsible for this sodium acquisition, with the number of transcripts expressed being dependent on the number of mechano-electric stimulations. Hence, the number of APs a victim triggers while trying to break out of the trap identifies the moving prey as a struggling Na+-rich animal and nutrition for the plant.
Current Biology
10.1016/j.cub.2015.11.057
urn:nbn:de:bvb:20-opus-128054
Current Biology 26, 3, 286–295, 2016. DOI: 10.1016/j.cub.2015.11.057
250194
Jennifer Böhm
Sönke Scherzer
Elzbieta Krol
Ines Kreuzer
Katharina von Meyer
Christian Lorey
Thomas D. Mueller
Lana Shabala
Isabel Monte
Roberto Salano
Khaled A. S. Al-Rasheid
Heinz Rennenberg
Sergey Shabala
Erwin Neher
Rainer Hedrich
deu
swd
Venusfliegenfalle
deu
uncontrolled
Dionaea muscipula
Magnoliopsida (Zweikeimblättrige)
open_access
Julius-von-Sachs-Institut für Biowissenschaften
OpenAIRE
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/12805/Boehm_Current_Biology.pdf
6512
2012
deu
article
1
2013-03-27
--
--
Methods of staining and visualization of sphingolipid enriched and non-enriched plasma membrane regions of Arabidopsis thaliana with fluorescent dyes and lipid analogues
Background: Sterols and Sphingolipids form lipid clusters in the plasma membranes of cell types throughout the animal and plant kingdoms. These lipid domains provide a medium for protein signaling complexes at the plasma membrane and are also observed to be principal regions of membrane contact at the inception of infection. We visualized different specific fluorescent lipophilic stains of the both sphingolipid enriched and non-sphingolipid enriched regions in the plasma membranes of live protoplasts of Arabidopsis thaliana. Results: Lipid staining protocols for several fluorescent lipid analogues in plants are presented. The most emphasis was placed on successful protocols for the single and dual staining of sphingolipid enriched regions and exclusion of sphingolipid enriched regions on the plasma membrane of Arabidopsis thaliana protoplasts. A secondary focus was placed to ensure that these staining protocols presented still maintain cell viability. Furthermore, the protocols were successfully tested with the spectrally sensitive dye Laurdan. Conclusion: Almost all existing staining procedures of the plasma membrane with fluorescent lipid analogues are specified for animal cells and tissues. In order to develop lipid staining protocols for plants, procedures were established with critical steps for the plasma membrane staining of Arabidopsis leaf tissue and protoplasts. The success of the plasma membrane staining protocols was additionally verified by measurements of lipid dynamics by the fluorescence recovery after photobleaching technique and by the observation of new phenomena such as time dependent lipid polarization events in living protoplasts, for which a putative physiological relevance is suggested.
urn:nbn:de:bvb:20-opus-75433
7543
In: Plant Methods (2012) 8: 28, doi:10.1186/1746-4811-8-28
Jörg O. Blachutzik
Faith Demir
Ines Kreuzer
Rainer Hedrich
Gregory S. Harms
deu
swd
Arabidopsis thaliana
eng
uncontrolled
Protoplasts
eng
uncontrolled
Lipid polarization
eng
uncontrolled
Lipophilic fluorescent dyes
eng
uncontrolled
Laurdan
eng
uncontrolled
Sphingolipid
eng
uncontrolled
Liquid (dis-) ordered phase
eng
uncontrolled
Plasma membrane
eng
uncontrolled
Fluorescence mi
Pflanzen (Botanik)
open_access
Julius-von-Sachs-Institut für Biowissenschaften
Förderzeitraum 2012
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/6512/035_1746_4811_8_28.pdf
19087
2016
eng
286-295
3
26
article
1
2019-10-29
--
--
The Venus flytrap Dionaea muscipula counts prey-induced action potentials to induce sodium uptake
Carnivorous plants, such as the Venus flytrap (Dionaea muscipula), depend on an animal diet when grown in nutrient-poor soils. When an insect visits the trap and tilts the mechanosensors on the inner surface, action potentials (APs) are fired. After a moving object elicits two APs, the trap snaps shut, encaging the victim. Panicking preys repeatedly touch the trigger hairs over the subsequent hours, leading to a hermetically closed trap, which via the gland-based endocrine system is flooded by a prey-decomposing acidic enzyme cocktail. Here, we asked the question as to how many times trigger hairs have to be stimulated (e.g., now many APs are required) for the flytrap to recognize an encaged object as potential food, thus making it worthwhile activating the glands. By applying a series of trigger-hair stimulations, we found that the touch hormone jasmonic acid (JA) signaling pathway is activated after the second stimulus, while more than three APs are required to trigger an expression of genes encoding prey-degrading hydrolases, and that this expression is proportional to the number of mechanical stimulations. A decomposing animal contains a sodium load, and we have found that these sodium ions enter the capture organ via glands. We identified a flytrap sodium channel DmHKT1 as responsible for this sodium acquisition, with the number of transcripts expressed being dependent on the number of mechano-electric stimulations. Hence, the number of APs a victim triggers while trying to break out of the trap identifies the moving prey as a struggling Na\(^+\)-rich animal and nutrition for the plant.
Current Biology
10.1016/j.cub.2015.11.057
urn:nbn:de:bvb:20-opus-190870
Current Biology (2016) 26:3, S. 286-295. https://doi.org/10.1016/j.cub.2015.11.057
250194
true
true
CC BY-NC-ND: Creative-Commons-Lizenz: Namensnennung, Nicht kommerziell, Keine Bearbeitungen 4.0 International
Jennifer Böhm
Sönke Scherzer
Elzbieta Krol
Ines Kreuzer
Katharina von Meyer
Christian Lorey
Thomas D. Mueller
Lana Shabala
Isabel Monte
Roberto Solano
Khaled A. S. Al-Rasheid
Heinz Rennenberg
Sergey Shabala
Erwin Neher
Rainer Hedrich
eng
uncontrolled
jasmonic acid biosynthesis
eng
uncontrolled
gene expression
eng
uncontrolled
signal transduction
eng
uncontrolled
transporters
eng
uncontrolled
Arabidopsis
Physiologie und verwandte Themen
open_access
Julius-von-Sachs-Institut für Biowissenschaften
OpenAIRE
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/19087/Boehm_CurrentBiology_2016.pdf
18879
2016
eng
812-825
6
26
article
1
2019-10-15
--
--
Venus flytrap carnivorous lifestyle builds on herbivore defense strategies
Although the concept of botanical carnivory has been known since Darwin's time, the molecular mechanisms that allow animal feeding remain unknown, primarily due to a complete lack of genomic information. Here, we show that the transcriptomic landscape of the Dionaea trap is dramatically shifted toward signal transduction and nutrient transport upon insect feeding, with touch hormone signaling and protein secretion prevailing. At the same time, a massive induction of general defense responses is accompanied by the repression of cell death-related genes/processes. We hypothesize that the carnivory syndrome of Dionaea evolved by exaptation of ancient defense pathways, replacing cell death with nutrient acquisition.
Genome Research
10.1101/gr.202200.115
urn:nbn:de:bvb:20-opus-188799
Genome Research (2016) 26:6, 812-825. https://doi.org/10.1101/gr.202200.115
250194-Carnivorom
false
true
CC BY-NC: Creative-Commons-Lizenz: Namensnennung, Nicht kommerziell 4.0 International
Felix Bemm
Dirk Becker
Christina Larisch
Ines Kreuzer
Maria Escalante-Perez
Waltraud X. Schulze
Markus Ankenbrand
Anna-Lena Van de Weyer
Elzbieta Krol
Khaled A. Al-Rasheid
Axel Mithöfer
Andreas P. Weber
Jörg Schultz
Rainer Hedrich
eng
uncontrolled
Dionaea-muscipula ellis
eng
uncontrolled
Plant utricularia-gibba
eng
uncontrolled
Programmed cell-death
eng
uncontrolled
Genomics data sets
eng
uncontrolled
RNA-SEQ data
eng
uncontrolled
Arabidopsis-thaliana
eng
uncontrolled
Jasmonate perception
eng
uncontrolled
Action potentials
eng
uncontrolled
Stress responses
eng
uncontrolled
Wonderful plants
Biowissenschaften; Biologie
open_access
Theodor-Boveri-Institut für Biowissenschaften
OpenAIRE
Center for Computational and Theoretical Biology
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/18879/Bemm_GenomeResearch_2016.pdf
30041
2022
eng
12
article
1
--
--
--
Ether anesthetics prevents touch-induced trigger hair calcium-electrical signals excite the Venus flytrap
Plants do not have neurons but operate transmembrane ion channels and can get electrical excited by physical and chemical clues. Among them the Venus flytrap is characterized by its peculiar hapto-electric signaling. When insects collide with trigger hairs emerging the trap inner surface, the mechanical stimulus within the mechanosensory organ is translated into a calcium signal and an action potential (AP). Here we asked how the Ca\(^{2+}\) wave and AP is initiated in the trigger hair and how it is feed into systemic trap calcium-electrical networks. When Dionaea muscipula trigger hairs matures and develop hapto-electric excitability the mechanosensitive anion channel DmMSL10/FLYC1 and voltage dependent SKOR type Shaker K\(^{+}\) channel are expressed in the sheering stress sensitive podium. The podium of the trigger hair is interface to the flytrap’s prey capture and processing networks. In the excitable state touch stimulation of the trigger hair evokes a rise in the podium Ca2+ first and before the calcium signal together with an action potential travel all over the trap surface. In search for podium ion channels and pumps mediating touch induced Ca\(^{2+}\) transients, we, in mature trigger hairs firing fast Ca\(^{2+}\) signals and APs, found OSCA1.7 and GLR3.6 type Ca\(^{2+}\) channels and ACA2/10 Ca\(^{2+}\) pumps specifically expressed in the podium. Like trigger hair stimulation, glutamate application to the trap directly evoked a propagating Ca\(^{2+}\) and electrical event. Given that anesthetics affect K\(^+\) channels and glutamate receptors in the animal system we exposed flytraps to an ether atmosphere. As result propagation of touch and glutamate induced Ca\(^{2+}\) and AP long-distance signaling got suppressed, while the trap completely recovered excitability when ether was replaced by fresh air. In line with ether targeting a calcium channel addressing a Ca\(^{2+}\) activated anion channel the AP amplitude declined before the electrical signal ceased completely. Ether in the mechanosensory organ did neither prevent the touch induction of a calcium signal nor this post stimulus decay. This finding indicates that ether prevents the touch activated, glr3.6 expressing base of the trigger hair to excite the capture organ.
Scientific reports
10.1038/s41598-022-06915-z
urn:nbn:de:bvb:20-opus-300411
@articleScherzer.2022, author = Scherzer, Sönke and Huang, Shouguang and Iosip, Anda and Kreuzer, Ines and Yokawa, Ken and Al-Rasheid, Khaled A. S. and Heckmann, Manfred and Hedrich, Rainer, year = 2022, title = Ether anesthetics prevents touch-induced trigger hair calcium-electrical signals excite the Venus flytrap, pages = 2851, volume = 12, number = 1, journal = Scientific reports, doi = 10.1038/s41598-022-06915-z,
md5:d404ac54732da953260c61b64caa9578
2023-01-20T07:08:58+00:00
/tmp/phpOy2dZl
bibtex
63ca3e0a953c80.48508011
Scientific Reports (2022) 12:2851. https://doi.org/10.1038/s41598-022-06915-z
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Sönke Scherzer
Shouguang Huang
Anda Iosip
Ines Kreuzer
Ken Yokawa
Khaled A. S. Al-Rasheid
Manfred Heckmann
Rainer Hedrich
eng
uncontrolled
biophysics
eng
uncontrolled
drug discovery
eng
uncontrolled
physiology
eng
uncontrolled
plan sciences
Biowissenschaften; Biologie
open_access
Physiologisches Institut
Julius-von-Sachs-Institut für Biowissenschaften
Förderzeitraum 2022
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/30041/s41598-022-06915-z.pdf