14638
2016
eng
30
16
article
1
2017-03-30
--
--
3D rotational fluoroscopy for intraoperative clip control in patients with intracranial aneurysms – assessment of feasibility and image quality
Background
Mobile 3D fluoroscopes have become increasingly available in neurosurgical operating rooms. In this series, the image quality and value of intraoperative 3D fluoroscopy with intravenous contrast agent for the evaluation of aneurysm occlusion and vessel patency after clip placement was assessed in patients who underwent surgery for intracranial aneurysms.
Materials and methods
Twelve patients were included in this retrospective analysis. Prior to surgery, a 360° rotational fluoroscopy scan was performed without contrast agent followed by another scan with 50 ml of intravenous iodine contrast agent. The image files of both scans were transferred to an Apple PowerMac® workstation, subtracted and reconstructed using OsiriX® free software. The procedure was repeated after clip placement. Both image sets were compared for assessment of aneurysm occlusion and vessel patency.
Results
Image acquisition and contrast administration caused no adverse effects. Image quality was sufficient to follow the patency of the vessels distal to the clip. Metal artifacts reduce the assessability of the immediate vicinity of the clip. Precise image subtraction and post-processing can reduce metal artifacts and make the clip-site assessable and depict larger neck-remnants.
Conclusion
This technique quickly supplies images at adequate quality to evaluate distal vessel patency after aneurysm clipping. Significant aneurysm remnants may be depicted as well. As it does not require visual control of all vessels that are supposed to be evaluated intraoperatively, this technique may be complementary to other intraoperative tools like indocyanine green videoangiography and micro-Doppler, especially for the assessment of larger aneurysms. At the momentary state of this technology, it cannot replace postoperative conventional angiography. However, 3D fluoroscopy and image post-processing are young technologies. Further technical developments are likely to result in improved image quality.
BMC Medical Imaging
10.1186/s12880-016-0133-0
urn:nbn:de:bvb:20-opus-146381
BMC Medical Imaging (2016) 16:30 DOI 10.1186/s12880-016-0133-0
Thomas Westermaier
Thomas Linsenmann
György A. Homola
Mario Loehr
Christian Stetter
Nadine Willner
Ralf-Ingo Ernestus
Laszlo Soymosi
Giles H. Vince
eng
uncontrolled
aneurysm surgery
eng
uncontrolled
clip control
eng
uncontrolled
angiography
eng
uncontrolled
3D fluoroscopy
eng
uncontrolled
image quality
eng
uncontrolled
intraoperative
eng
uncontrolled
vessel patency
eng
uncontrolled
contrast
eng
uncontrolled
post-processing
Chirurgie und verwandte medizinische Fachrichtungen
open_access
Neurologische Klinik und Poliklinik
Institut für diagnostische und interventionelle Neuroradiologie (ehem. Abteilung für Neuroradiologie)
Förderzeitraum 2016
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/14638/Westermaier_10.1186_s12880-016-0133-0.pdf
17754
2018
eng
8673-8684
11
article
1
2019-02-27
--
--
Expression of activating transcription factor 5 (ATF5) is increased in astrocytomas of different WHO grades and correlates with survival of glioblastoma patients
Background:
ATF5 suppresses differentiation of neuroprogenitor cells and is overexpressed in glioblastoma (GBM). A reduction of its expression leads to apoptotic GBM cell death. Data on ATF5 expression in astrocytoma WHO grade II (low-grade astrocytoma [LGA]) are scarce and lacking on recurrent GBM.
Patients and methods:
ATF5 mRNA was extracted from frozen samples of patients’ GBM (n=79), LGA (n=40), and normal brain (NB, n=10), quantified by duplex qPCR and correlated with retrospectively collected clinical data. ATF5 protein expression was evaluated by measuring staining intensity on immunohistochemistry.
Results:
ATF5 mRNA was overexpressed in LGA (sevenfold, P<0.001) and GBM (tenfold, P<0.001) compared to NB, which was confirmed on protein level. Although ATF5 mRNA expression in GBM showed a considerable fluctuation range, groups of varying biological behavior, that is, local/multifocal growth or primary tumor/relapse and the tumor localization at diagnosis, were not significantly different. ATF5 mRNA correlated with the patients’ age (r=0.339, P=0.028) and inversely with Ki67-staining (r=-0.421, P=0.007). GBM patients were allocated to a low and a high ATF5 expression group by the median ATF5 overexpression compared to NB. Kaplan–Meier analysis and Cox regression indicated that ATF5 mRNA expression significantly correlated with short-term survival (t<12 months, median survival 18 vs 13 months, P=0.022, HR 2.827) and progression-free survival (PFS) (12 vs 6 months, P=0.024). This advantage vanished after 24 months (P=0.084).
Conclusion:
ATF5 mRNA expression could be identified as an additional, though not independent factor correlating with overall survival and PFS. Since its inhibition might lead to the selective death of glioma cells, it might serve as a potential ubiquitous therapeutic target in astrocytic tumors.
OncoTargets and Therapy
10.2147/OTT.S176549
urn:nbn:de:bvb:20-opus-177541
OncoTargets and Therapy 2018, 11, 8673-8684. DOI: 10.2147/OTT.S176549
false
true
Jonas Feldheim
Almuth F Kessler
Dominik Schmitt
Lara Wilczek
Thomas Linsenmann
Mathias Dahlmann
Camelia M Monoranu
Ralf-Ingo Ernestus
Carsten Hagemann
Mario Löhr
eng
uncontrolled
glioblastoma multiforme
eng
uncontrolled
recurrence
eng
uncontrolled
growth pattern
eng
uncontrolled
protein and mRNA expression
Medizin und Gesundheit
open_access
Pathologisches Institut
Neurologische Klinik und Poliklinik
Förderzeitraum 2018
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/17754/Feldheim_OncoTargets_and_Therapy.pdf
12577
2015
eng
498936
2015
article
1
2016-01-27
--
--
Intraoperative Myelography in Cervical Multilevel Stenosis Using 3D Rotational Fluoroscopy: Assessment of Feasibility and Image Quality
Background. Intraoperative myelography has been reported for decompression control in multilevel lumbar disease. Cervical myelography is technically more challenging. Modern 3D fluoroscopy may provide a new opportunity supplying multiplanar images. This study was performed to determine the feasibility and image quality of intraoperative cervical myelography using a 3D fluoroscope. Methods. The series included 9 patients with multilevel cervical stenosis. After decompression, 10 mL of water-soluble contrast agent was administered via a lumbar drainage and the operating table was tilted. Thereafter, a 3D fluoroscopy scan (O-Arm) was performed and visually evaluated. Findings. The quality of multiplanar images was sufficient to supply information about the presence of residual stenosis. After instrumentation, metal artifacts lowered image quality. In 3 cases, decompression was continued because myelography depicted residual stenosis. In one case, anterior corpectomy was not completed because myelography showed sufficient decompression after 2-level discectomy. Interpretation. Intraoperative myelography using 3D rotational fluoroscopy is useful for the control of surgical decompression in multilevel spinal stenosis providing images comparable to postmyelographic CT. The long duration of contrast delivery into the cervical spine may be solved by preoperative contrast administration. The method is susceptible to metal artifacts and, therefore, should be applied before metal implants are placed.
Radiology Research and Practice
10.1155/2015/498936
urn:nbn:de:bvb:20-opus-125779
Radiology Research and Practice Volume 2015, Article ID 498936, 7 pages. DOI: 10.1155/2015/498936
Thomas Westermaier
Stefan Koehler
Thomas Linsenmann
Michael Kinderlen
Paul Pakos
Ralf-Ingo Ernestus
Medizin und Gesundheit
open_access
Neurologische Klinik und Poliklinik
Förderzeitraum 2015
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/12577/Westermaier_498936.pdf
17765
2019
eng
7-11
16
article
1
2019-02-28
--
--
Cerebellar liponeurocytoma - molecular signature of a rare entity and the importance of an accurate diagnosis
Background:
Cerebellar liponeurocytoma is an extremely rare tumour entity of the central nervous system. It is histologically characterised by prominent neuronal/neurocytic differentiation with focal lipidisation and corresponding histologically to WHO grade II. It typically develops in adults, and usually shows a low proliferative potential. Recurrences have been reported in almost 50% of cases, and in some cases the recurrent tumour may display increased mitotic activity and proliferation index, vascular proliferations and necrosis. Thus pathological diagnosis of liponeurocytoma is challenging. This case presentation highlights the main clinical, radiographic and pathological features of a cerebellar liponeurocytoma.
Case presentation:
A 59-year-old, right-handed woman presented at our department with a short history of persistent headache, vertigo and gait disturbances. Examination at presentation revealed that the patient was awake, alert and fully oriented. The cranial nerve status was normal. Uncertainties were noted in the bilateral finger-to-nose testing with bradydiadochokinesis on both sides. Strength was full and no pronator drift was observed. Sensation was intact. No signs of pyramidal tract dysfunction were detected. Her gait appeared insecure. The patient underwent surgical resection. Afterward no further disturbances could be detected.
Conclusions:
To date >40 cases of liponeurocytoma have been reported, including cases with supratentorial location. A review of the 5 published cases of recurrent cerebellar. Liponeurocytoma revealed that the median interval between the first and second relapse was rather short, indicating uncertain malignant potential. The most recent WHO classification of brain tumours (2016) classifies the cerebellar liponeurocytoma as a separate entity and assigns the tumour to WHO grade II. Medulloblastoma is the most important differential diagnosis commonly seen in children and young adults. In contrast, cerebellar liponeurocytoma is typically diagnosed in adults. The importance of accurate diagnosis should not be underestimated especially in the view of possible further therapeutic interventions and for the determination of the patient's prognosis.
Interdisciplinary Neurosurgery
10.1016/j.inat.2018.10.017
urn:nbn:de:bvb:20-opus-177652
Interdisciplinary Neurosurgery 16 (2019) 7-11. DOI: 10.1016/j.inat.2018.10.017
false
true
CC BY-NC-ND: Creative-Commons-Lizenz: Namensnennung, Nicht kommerziell, Keine Bearbeitungen 4.0 International
Thomas Linsenmann
Camelia M. Monoranu
Balint Alkonyi
Thomas Westermaier
Carsten Hagemann
Almuth F. Kessler
Ralf-Ingo Ernestus
Mario Löhr
eng
uncontrolled
liponeurocytoma
eng
uncontrolled
neurocytoma
eng
uncontrolled
medulloblastoma
eng
uncontrolled
molecular signature
Medizin und Gesundheit
open_access
Neurochirurgische Klinik und Poliklinik
Neurologische Klinik und Poliklinik
Förderzeitraum 2018
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/17765/Linsenmann_Interdisciplinary_Neurosurgery.pdf