14407
2015
eng
8
13
article
1
2017-02-08
--
--
The proteome profiles of the olfactory bulb of juvenile, adult and aged rats - an ontogenetic study
Background:
In this study, we searched for proteins that change their expression in the olfactory bulb (oB) of rats during ontogenesis. Up to now, protein expression differences in the developing animal are not fully understood. Our investigation focused on the question whether specific proteins exist which are only expressed during different development stages. This might lead to a better characterization of the microenvironment and to a better determination of factors and candidates that influence the differentiation of neuronal progenitor cells.
Results:
After analyzing the samples by two-dimensional polyacrylamide gel electrophoresis (2DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), it could be shown that the number of expressed proteins differs depending on the developmental stages. Especially members of the functional classes, like proteins of biosynthesis, regulatory proteins and structural proteins, show the highest differential expression in the stages of development analyzed.
Conclusion:
In this study, quantitative changes in the expression of proteins in the oB at different developmental stages (postnatal days (P) 7, 90 and 637) could be observed. Furthermore, the expression of many proteins was found at specific developmental stages. It was possible to identify these proteins which are involved in processes like support of cell migration and differentiation.
Proteome Science
10.1186/s12953-014-0058-x
urn:nbn:de:bvb:20-opus-144073
Proteome Science (2015) 13:8. DOI: 10.1186/s12953-014-0058-x
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Michael Wille
Antje Schümann
Michael Kreutzer
Michael O Glocker
Andreas Wree
Grit Mutzbauer
Oliver Schmitt
eng
uncontrolled
axonally transported proteins
eng
uncontrolled
hippocampal stem cells
eng
uncontrolled
cerebral cortex
eng
uncontrolled
regional development
eng
uncontrolled
development
eng
uncontrolled
brain
eng
uncontrolled
olfactory bulb
eng
uncontrolled
proteomics
eng
uncontrolled
rat
eng
uncontrolled
growth-associated protein
eng
uncontrolled
messenger-RNA transport
eng
uncontrolled
goldfish optic nerve
eng
uncontrolled
postnatal development
eng
uncontrolled
subventricular zone
eng
uncontrolled
neuronal differentiation
Medizin und Gesundheit
open_access
Pathologisches Institut
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/14407/053_Wille_Proteome_Science.pdf
15134
2015
eng
21454
21485
16
article
1
2017-07-11
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The Proteome Profiles of the Cerebellum of Juvenile, Adult and Aged Rats-An Ontogenetic Study
In this study, we searched for proteins that change their expression in the cerebellum (Ce) of rats during ontogenesis. This study focuses on the question of whether specific proteins exist which are differentially expressed with regard to postnatal stages of development. A better characterization of the microenvironment and its development may result from these study findings. A differential two-dimensional polyacrylamide gel electrophoresis (2DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis of the samples revealed that the number of proteins of the functional classes differed depending on the developmental stages. Especially members of the functional classes of biosynthesis, regulatory proteins, chaperones and structural proteins show the highest differential expression within the analyzed stages of development. Therefore, members of these functional protein groups seem to be involved in the development and differentiation of the Ce within the analyzed development stages. In this study, changes in the expression of proteins in the Ce at different postnatal developmental stages (postnatal days (P) 7, 90, and 637) could be observed. At the same time, an identification of proteins which are involved in cell migration and differentiation was possible. Especially proteins involved in processes of the biosynthesis and regulation, the dynamic organization of the cytoskeleton as well as chaperones showed a high amount of differentially expressed proteins between the analyzed dates.
International Journal of Molecular Sciences
10.3390/ijms160921454
urn:nbn:de:bvb:20-opus-151347
International Journal of Molecular Sciences 2015, 16, 21454-21485. DOI:10.3390/ijms160921454
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Michael Wille
Antje Schümann
Andreas Wree
Michael Kreutzer
Michael O. Glocker
Grit Mutzbauer
Oliver Schmitt
eng
uncontrolled
messenger RNA
eng
uncontrolled
brain
eng
uncontrolled
cerebellum
eng
uncontrolled
development
eng
uncontrolled
proteomics
eng
uncontrolled
rat
eng
uncontrolled
proteins
eng
uncontrolled
adenosine kinase
eng
uncontrolled
coated vesicles
eng
uncontrolled
phosphatase 2A
eng
uncontrolled
expression
eng
uncontrolled
neuronal differentiation
eng
uncontrolled
human brain
eng
uncontrolled
hnRNP K
eng
uncontrolled
postnatal development
eng
uncontrolled
binding
Medizin und Gesundheit
open_access
Pathologisches Institut
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/15134/001_Wille_INTERNATIONAL_JOURNAL_OF_MOLECULAR_SCIENCES.pdf