15953
2017
eng
537
17
article
1
2018-03-22
--
--
Cellular pharmacodynamic effects of Pycnogenol\(^{®}\) in patients with severe osteoarthritis: a randomized controlled pilot study
Background:
The standardized maritime pine bark extract (Pycnogenol\(^{®}\)) has previously shown symptom alleviating effects in patients suffering from moderate forms of knee osteoarthritis (OA). The cellular mechanisms for this positive impact are so far unknown. The purpose of the present randomized pilot controlled study was to span the knowledge gap between the reported clinical effects of Pycnogenol\(^{®}\) and its in vivo mechanism of action in OA patients.
Methods:
Thirty three patients with severe OA scheduled for a knee arthroplasty either received 100 mg of Pycnogenol\(^{®}\) twice daily or no treatment (control group) three weeks before surgery. Cartilage, synovial fluid and serum samples were collected during surgical intervention. Relative gene expression of cartilage homeostasis markers were analyzed in the patients' chondrocytes. Inflammatory and cartilage metabolism mediators were investigated in serum and synovial fluid samples.
Results:
The oral intake of Pycnogenol\(^{®}\) downregulated the gene expression of various cartilage degradation markers in the patients' chondrocytes, the decrease of MMP3, MMP13 and the pro-inflammatory cytokine IL1B were statistically significant (p ≤ 0.05). Additionally, protein concentrations of ADAMTS-5 in serum were reduced significantly (p ≤ 0.05) after three weeks intake of the pine bark extract.
Conclusions:
This is the first report about positive cellular effects of a dietary supplement on key catabolic and inflammatory markers in patients with severe OA. The results provide a rational basis for understanding previously reported clinical effects of Pycnogenol\(^{®}\) on symptom scores of patients suffering from OA.
BMC Complementary and Alternative Medicine
10.1186/s12906-017-2044-1
urn:nbn:de:bvb:20-opus-159532
BMC Complementary and Alternative Medicine (2017) 17:537. DOI: 10.1186/s12906-017-2044-1
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Steffen Jessberger
Petra Högger
Franca Genest
Donald M. Salter
Lothar Seefried
eng
uncontrolled
maritime pine bark extract
eng
uncontrolled
qPCR
eng
uncontrolled
ADAMTS
eng
uncontrolled
cartilage
eng
uncontrolled
clinical study
eng
uncontrolled
osteoarthritis
eng
uncontrolled
Pycnogenol
eng
uncontrolled
serum
eng
uncontrolled
synovial fluid
Krankheiten
open_access
Lehrstuhl für Orthopädie
Institut für Pharmazie und Lebensmittelchemie
Förderzeitraum 2017
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/15953/Jessberger_BMC Complementary_and_Alternative_Medicine.pdf
15986
2017
eng
443
5
9
article
1
2018-03-29
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--
Distribution of constituents and metabolites of maritime pine bark extract (Pycnogenol\(^{®}\)) into serum, blood cells, and synovial fluid of patients with severe osteoarthritis: a randomized controlled trial
The present randomized controlled study aimed to investigate the in vivo distribution of constituents or metabolites of the standardized maritime pine bark extract Pycnogenol\(^{®}\). Thirty-three patients with severe osteoarthritis scheduled for a knee arthroplasty were randomized to receive either 200 mg per day Pycnogenol\(^{®}\) (P+) or no treatment (Co) over three weeks before surgery. Serum, blood cells, and synovial fluid samples were analyzed using liquid chromatography coupled to tandem mass spectrometry with electrospray ionization (LC-ESI/MS/MS). Considerable interindividual differences were observed indicating pronounced variability of the polyphenol pharmacokinetics. Notably, the highest polyphenol concentrations were not detected in serum. Catechin and taxifolin primarily resided within the blood cells while the microbial catechin metabolite δ-(3,4-dihydroxy-phenyl)-γ-valerolactone, ferulic, and caffeic acid were mainly present in synovial fluid samples. Taxifolin was detected in serum and synovial fluid exclusively in the P+ group. Likewise, no ferulic acid was found in serum samples of the Co group. Calculating ratios of analyte distribution in individual patients revealed a simultaneous presence of some polyphenols in serum, blood cells, and/or synovial fluid only in the P+ group. This is the first evidence that polyphenols distribute into the synovial fluid of patients with osteoarthritis which supports rationalizing the results of clinical efficacy studies.
Nutrients
10.3390/nu9050443
urn:nbn:de:bvb:20-opus-159862
Nutrients 2017, 9(5), 443. DOI: 10.3390/nu9050443
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Melanie Mülek
Lothar Seefried
Franca Genest
Petra Högger
eng
uncontrolled
osteoarthritis
eng
uncontrolled
randomized controlled study
eng
uncontrolled
LC-ESI/MS/MS
eng
uncontrolled
human
eng
uncontrolled
pine bark extract
eng
uncontrolled
polyphenols
Krankheiten
open_access
Institut für Pharmazie und Lebensmittelchemie
Förderzeitraum 2017
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/15986/Mülek_Nutrients.pdf