17817
2016
eng
9
12
article
1
2019-03-08
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Allatostatin A Signalling in Drosophila Regulates Feeding and Sleep and Is Modulated by PDF
Feeding and sleep are fundamental behaviours with significant interconnections and cross-modulations. The circadian system and peptidergic signals are important components of this modulation, but still little is known about the mechanisms and networks by which they interact to regulate feeding and sleep. We show that specific thermogenetic activation of peptidergic Allatostatin A (AstA)-expressing PLP neurons and enteroendocrine cells reduces feeding and promotes sleep in the fruit fly Drosophila. The effects of AstA cell activation are mediated by AstA peptides with receptors homolog to galanin receptors subserving similar and apparently conserved functions in vertebrates. We further identify the PLP neurons as a downstream target of the neuropeptide pigment-dispersing factor (PDF), an output factor of the circadian clock. PLP neurons are contacted by PDF-expressing clock neurons, and express a functional PDF receptor demonstrated by cAMP imaging. Silencing of AstA signalling and continuous input to AstA cells by tethered PDF changes the sleep/activity ratio in opposite directions but does not affect rhythmicity. Taken together, our results suggest that pleiotropic AstA signalling by a distinct neuronal and enteroendocrine AstA cell subset adapts the fly to a digestive energy-saving state which can be modulated by PDF.
PLoS Genetics
10.1371/journal.pgen.1006346
urn:nbn:de:bvb:20-opus-178170
PLoS Genetics 2016, 12(9):e1006346. DOI: 10.1371/journal.pgen.1006346
false
true
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Jiangtian Chen
Wencke Reiher
Christiane Hermann-Luibl
Azza Sellami
Paola Cognigni
Shu Kondo
Charlotte Helfrich-Förster
Jan A. Veenstra
Christian Wegener
eng
uncontrolled
neurons
eng
uncontrolled
neuroimaging
eng
uncontrolled
circadian rhythms
eng
uncontrolled
food consumption
eng
uncontrolled
sleep
eng
uncontrolled
biological locomotion
eng
uncontrolled
Drosophila melanogaster
eng
uncontrolled
signal peptides
Biowissenschaften; Biologie
open_access
Theodor-Boveri-Institut für Biowissenschaften
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/17817/Chen_Plos_Genetics.pdf
19594
2018
eng
09
article
1
--
2018-09-10
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The CCHamide1 Neuropeptide Expressed in the Anterior Dorsal Neuron 1 Conveys a Circadian Signal to the Ventral Lateral Neurons in Drosophila melanogaster
The fruit fly Drosophila melanogaster possesses approximately 150 brain clock neurons that control circadian behavioral rhythms. Even though individual clock neurons have self-sustaining oscillators, they interact and synchronize with each other through a network. However, little is known regarding the factors responsible for these network interactions. In this study, we investigated the role of CCHamide1 (CCHa1), a neuropeptide expressed in the anterior dorsal neuron 1 (DN1a), in intercellular communication of the clock neurons. We observed that CCHa1 connects the DN1a clock neurons to the ventral lateral clock neurons (LNv) via the CCHa1 receptor, which is a homolog of the gastrin-releasing peptide receptor playing a role in circadian intercellular communications in mammals. CCHa1 knockout or knockdown flies have a generally low activity level with a special reduction of morning activity. In addition, they exhibit advanced morning activity under light-dark cycles and delayed activity under constant dark conditions, which correlates with an advance/delay of PAR domain Protein 1 (PDP1) oscillations in the small-LNv (s-LNv) neurons that control morning activity. The terminals of the s-LNv neurons show rather high levels of Pigment-dispersing factor (PDF) in the evening, when PDF is low in control flies, suggesting that the knockdown of CCHa1 leads to increased PDF release; PDF signals the other clock neurons and evidently increases the amplitude of their PDP1 cycling. A previous study showed that high-amplitude PDP1 cycling increases the siesta of the flies, and indeed, CCHa1 knockout or knockdown flies exhibit a longer siesta than control flies. The DN1a neurons are known to be receptive to PDF signaling from the s-LNv neurons; thus, our results suggest that the DN1a and s-LNv clock neurons are reciprocally coupled via the neuropeptides CCHa1 and PDF, and this interaction fine-tunes the timing of activity and sleep.
Frontiers in Physiology
1664-042X
10.3389/fphys.2018.01276
urn:nbn:de:bvb:20-opus-195940
Frontiers in Physiology 2018, 9:1276. doi: 10.3389/fphys.2018.01276
true
true
CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International
Yuri Fujiwara
Christiane Hermann-Luibl
Maki Katsura
Manabu Sekiguchi
Takanori Ida
Charlotte Helfrich-Förster
Taishi Yoshii
eng
uncontrolled
circadian clock
eng
uncontrolled
circadian rhythm
eng
uncontrolled
CCHamide1
eng
uncontrolled
pacemaker neuron
eng
uncontrolled
neuropeptide
eng
uncontrolled
pigment-dispersing factor
Biowissenschaften; Biologie
open_access
Theodor-Boveri-Institut für Biowissenschaften
Import
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/19594/fphys-09-01276.pdf