6200
2010
eng
article
1
2012-10-11
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Disturbed expression of the T-cell receptor/CD3 complex and associated signaling molecules in CD30(+) T-cell lymphoproliferations
Background CD30+ T-cell lymphoproliferations comprise a spectrum of clinically heterogeneous entities, including systemic anaplastic large cell lymphomas (ALK- and ALK+) and primary cutaneous CD30+ T-cell lymphoproliferative disorders. While all these entities are characterized by proliferation of highly atypical, anaplastic CD30+ T cells, the expression of T-cell specific antigens in the tumor cells is not consistently detectable. Design and Methods We evaluated biopsies from 19 patients with primary cutaneous CD30+ lymphoproliferative disorders, 38 with ALK- and 33 with ALK+ systemic anaplastic large cell lymphoma. The biopsies were examined for the expression of T-cell receptoraβ/CD3 complex (CD3γ, δ, ε, ζ), transcription factors regulating T-cell receptor expression (ATF1, ATF2, TCF-1, TCF-1a/LEF-1, Ets1), and molecules of T-cell receptor-associated signaling cascades (Lck, ZAP-70, LAT, bcl-10, Carma1, NFATc1, c-Jun, c-Fos, Syk) using immunohistochemistry. Results In comparison to the pattern in 20 peripheral T-cell lymphomas, not otherwise specified, we detected a highly disturbed expression of the T-cell receptor/CD3 complex, TCF-1, TCF- 1a/LEF-1, Lck, ZAP-70, LAT, NFATc1, c-Jun, c-Fos and Syk in most of the systemic anaplastic large cell lymphomas. In addition, primary cutaneous CD30+ lymphoproliferative disorders showed such a similar expression pattern to that of systemic anaplastic large cell lymphomas, that none of the markers we investigated can reliably distinguish between these CD30+ T-cell lymphoproliferations. Conclusions Severely altered expression of the T-cell receptor/CD3 complex, T-cell receptor-associated transcription factors and signal transduction molecules is a common characteristic of systemic and cutaneous CD30+ lymphoproliferations, although the clinical behavior of these entities is very different. Since peripheral T-cell lymphomas, not otherwise specified retain the full expression program required for functioning T-cell receptor signaling, the differential expression of a subset of these markers might be of diagnostic utility in distinguishing peripheral T-cell lymphomas, not otherwise specified from the entire group of CD30+ lymphoproliferations.
urn:nbn:de:bvb:20-opus-68179
6817
In: Haematologica - The hematology journal (2010) 95, 10, 1697-1704, DOI: 10.3324/haematol.2009.021428
Eva Geissinger
Petra Sadler
Sabine Roth
Tina Grieb
Bernhard Puppe
Nora Mueller
Peter Reimer
Claudia S. Vetter-Kauczok
Joerg Wenzel
Irina Bonzheim
Thomas Ruediger
Hans Konrad Mueller-Hermelink
Andreas Rosenwald
deu
swd
Medizin
eng
uncontrolled
systemic and cutaneous CD30+ lymphoproliferations
eng
uncontrolled
anaplastic large cell lymphoma
eng
uncontrolled
lymphomatoid papulosis
eng
uncontrolled
ALCL
eng
uncontrolled
LyP
eng
uncontrolled
TCR
Medizin und Gesundheit
open_access
Klinik und Poliklinik für Allgemein-, Viszeral-, Gefäß- und Kinderchirurgie (Chirurgische Klinik I)
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/6200/Geissinger042_1697.full.pdf
11139
2014
eng
article
1
2015-03-20
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p53 regulation by TRP2 is not pervasive in melanoma
p53 is a central tumor suppressor protein and its inhibition is believed to be a prerequisite for cancer development. In approximately 50% of all malignancies this is achieved by inactivating mutations in the p53 gene. However, in several cancer entities, including melanoma, p53 mutations are rare. It has been recently proposed that tyrosinase related protein 2 (TRP2), a protein involved in melanin synthesis, may act as suppressor of the p53 pathway in melanoma. To scrutinize this notion we analyzed p53 and TRP2 expression by immunohistochemistry in 172 melanoma tissues and did not find any correlation. Furthermore, we applied three different TRP2 shRNAs to five melanoma cell lines and could not observe a target specific effect of the TRP2 knockdown on either p53 expression nor p53 reporter gene activity. Likewise, ectopic expression of TRP2 in a TRP2 negative melanoma cell line had no impact on p53 expression. In conclusion our data suggest that p53 repression critically controlled by TRP2 is not a general event in melanoma.
10.1371/journal.pone.0087440
urn:nbn:de:bvb:20-opus-111396
PLoS ONE 9(1): e87440. doi:10.1371/journal.pone.0087440
Sebastian Haferkamp
Sonja Hesbacher
Gerhard Weyandt
Claudia S. Vetter-Kauczok
Jürgen C. Becker
Stephanie Motschenbacher
Marion Wobser
Melissa Maier
Corinna P. Schmid
Roland Houben
eng
uncontrolled
melanomas
eng
uncontrolled
melanoma cell
eng
uncontrolled
cell staining
eng
uncontrolled
histology
eng
uncontrolled
reporter genes
eng
uncontrolled
apoptosis
eng
uncontrolled
immunohistochemistry techniques
eng
uncontrolled
tumor suppressor genes
Medizin und Gesundheit
open_access
Klinik und Poliklinik für Dermatologie, Venerologie und Allergologie
Förderzeitraum 2014
Universität Würzburg
https://opus.bibliothek.uni-wuerzburg.de/files/11139/042_Haferkamp_PLoS.pdf